抗癌研究最新文献

{"title":"Bioinformatics Analysis and Experimental Verification of Prognostic and Biological Significance of Autophagy-Related Long Non-Coding RNAs in Gastric Carcinoma","authors":"Jiebang Jiang","doi":"10.26689/par.v7i1.4720","DOIUrl":"https://doi.org/10.26689/par.v7i1.4720","url":null,"abstract":"Background: Long non-coding RNAs (lncRNAs) play a vital role in autophagy modulation and tumor progression. However, the key lncRNAs and their functions in gastric cancer (GC) remain largely unknown. Methods: A bioinformatic analysis of GC patients’ gene expression profiling data from the Cancer Genome Atlas database was performed to identify autophagy-related lncRNAs that are associated with predictive risk. Through Cox regression and Lasso regression analyses, the autophagy-related lncRNAs that are associated with prognosis were identified, and a novel prognostic model for GC was established. The model was then used to evaluate the clinical features and predictive risk of individuals with GC. By using two datasets, GSE 62254 (n = 300) and GSE 15459 (n = 192), from Gene Expression Omnibus, its effectiveness was verified. Gene set enrichment analysis according to hallmark and Kyoto Encyclopedia of Genes and Genomes were used to determine the possible biological roles of these lncRNAs. Furthermore, the HOXD antisense growth-associated long non-coding RNA (HAGLR) mechanism in GC was discovered through in vitro and in vivo experiments. Results: Six lncRNAs associated with autophagy in GC were identified, and a new prognostic risk model based on these lncRNAs was established. The six-lncRNA signature was significantly associated with adverse clinicopathological features and found to be an independent GC prognostic factor. The model was proven to be effective and robust by GSE62254 and GSE15459. According to gene set enrichment analysis, the six lncRNAs appeared to be tightly linked to autophagy-related and cancer-related mechanisms. HAGLR was also found to promote tumor growth by enhancing autophagy signaling in GC. Conclusion: A novel prognostic model integrating HAGLR that can effectively evaluate and predict the prognostic risk of GC patients was established. The results indicated that HAGLR promotes gastric cancer progression by enhancing autophagy and is anticipated to be a potential new target for the treatment of gastric cancer.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45772426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Biomarkers for Multiple Myeloma","authors":"Chao Yang, J. Qi, Tianhong Jia, Jingping Ran, Wenxuan Cui","doi":"10.26689/par.v7i1.4681","DOIUrl":"https://doi.org/10.26689/par.v7i1.4681","url":null,"abstract":"Multiple myeloma (MM) is the second most common malignancy in hematology. MM is characterized by the malignant proliferation of plasma cells in the bone marrow, accompanied by the secretion of monoclonal immunoglobulin, mainly occurring in the elderly. The clinical manifestations of MM include renal dysfunction, bone destruction, infection, anemia, hemorrhage, hypercalcemia, and hyperviscosity syndrome. The recent discovery of biomarkers related to the diagnosis or prognosis of MM provides an important basis for the diagnosis and treatment of MM. This paper reviews the research progress of biomarkers expressed in tissues and peripheral blood at home and abroad.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46832747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effect of Integrated Traditional Chinese and Western Medicine on Anal Pruritus After Anorectal Surgery","authors":"F. Wu, J. Sha","doi":"10.26689/par.v7i1.4705","DOIUrl":"https://doi.org/10.26689/par.v7i1.4705","url":null,"abstract":"Objective: To explore the therapeutic effect of integrated traditional Chinese and western medicine on anal pruritus after anorectal surgery. Methods: Ninety-eight patients with anal pruritus after anorectal surgery in our hospital were selected as the research subjects. They were divided into two groups, the control group (50 cases) and the study group (48 cases), according to the treatment plan. The control group was under a simple western medicine treatment plan, while the study group was under an integrated traditional Chinese and western medicine treatment plan. The overall efficacy, severity of anal pruritus, time to eliminate clinical symptoms, and anxiety and depression scores of the two groups of patients under different treatment plans were compared. Results: After two weeks of treatment, the total effective rate of the study group was 95.83%, which was significantly higher than that of the control group (82.00%, P < 0.05). After 7 and 14 days of treatment, the anal pruritus scores improved significantly in both the groups, but the study group was superior to the control group, with statistical difference (P < 0.01). The time of disappearance of skin itching and skin damage in the study group was shorter than that in the control group. After 14 days of treatment, the anxiety and depression scores of both groups were lower than those after 7 days of treatment; however, there was statistical difference between the two groups (P < 0.01). Conclusion: In the clinical treatment of anorectal postoperative diseases, such as anal pruritus, the combination of traditional Chinese and western medicine can significantly improve the symptoms of pruritus, shorten the time of disappearance of clinical symptoms, improve depression and anxiety, and create a positive clinical application value in promoting the rehabilitation of patients and improving their quality of life.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45639898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mechanism of Long Non-Coding RNA SNHG7 in Cholangiocarcinoma Cell Proliferation, Migration, and Epithelial-Mesenchymal Transition","authors":"Ji-sen Zhao, Yang Yu, Yuanlong Zhou, Yuan Wang, Sheng Chen, Shujie Cheng","doi":"10.26689/par.v6i6.4452","DOIUrl":"https://doi.org/10.26689/par.v6i6.4452","url":null,"abstract":"Objective: To investigate the mechanism of long non-coding RNA SNHG7 and its regulatory effect on the proliferation, migration, and epithelial-mesenchymal transition of cholangiocarcinoma cells. Methods: A total of 20 pairs of cholangiocarcinoma and adjacent non-tumor bile duct tissues were collected from patients with cholangiocarcinoma who underwent surgery in the Affiliated Hospital of Hebei University (Hebei, China). Cholangiocarcinoma cell lines CCLP-1, QBC939, RBE, and HCC-9810 as well as normal human biliary epithelial cell line HIBEC were purchased for cell culture. We performed cell transfection, quantitative real-time polymerase chain reaction (qRT-PCR) to detect gene expression, Cell Counting Kit-8 (CCK-8) experiment to determine cell proliferation ability, scratch test to determine cell migration ability, and Transwell test to detect cell invasion ability. Results: The expression of lncRNA SNHG7 in cholangiocarcinoma cell lines CCLP-1, QBC939, RBE, and HCCC-9810 was 3.21 ± 1.01, 3.03 ± 1.02, 2.98 ± 1.21, and 3.12 ± 1.14, respectively, while its expression in normal cell line HIBEC was 3.21 ± 1.21; the expression of lncRNA SNHG7 in CCLP-1 was the highest; compared with HIBEC, the p values were all less than 0.05, indicating that the difference was statistically significant. The expression of miR-520f-3p in CCLP-1, QBC939, RBE, and HCCC-9810 was 1.45 ± 0.75, 1.55 ± 0.71, 1.54 ± 0.73, and 1.61 ± 0.72, respectively, while its expression in normal cell line HIBEC was 3.21 ± 1.21; the expression of miR-520f-3p in CCLP-1 was the lowest, and compared with HIBEC, the p values were all less than 0.05, indicating that the difference was statistically significant. In qRT-PCR, the expression of lncRNA SNHG7 of si-NC (3.21±1.11) was significantly higher than that of si-SNHG7 (1.14±0.76), and the p value was less than 0.05, indicating that the difference was statistically significant. In the CCK-8 experiment, the proliferation ability of CCLP-1 cells of the si-NC group at 24 h, 48 h, and 72 h was 0.61±0.59, 0.75±0.68, and 1.36±0.71, respectively; the proliferation ability of CCLP-1 cells of the si-SNHG7 group at 24 h, 48 h, and 72 h was 0.51±0.64, 0.59±0.59, and 0.63±0.61, respectively; there was a significant decrease in the proliferation ability, and the p value was less than 0.05, indicating a statistically significant difference. After 24 h of scratch treatment, compared with the si-NC group, the migration ability of CCLP-1 cells of the si-SNHG20 group was reduced (t = 6.356, P = 0.026). The results of Transwell test showed that the cell invasion ability of CCLP-1 in the si-SNHG20 group was significantly reduced compared with the si-NC group (t = 7.845, P = 0.032). Conclusion: Exploring the gene expression mechanism in relation to the occurrence and development of cholangiocarcinoma is beneficial to future clinical work in terms of diagnosis, treatment, and prognosis. The knockdown of lncRNA SNHG7 can effectively inhibit the proliferation, migra","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47338423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Painless Delivery Techniques on the Psychology of Primipara in Obstetrics Clinic","authors":"Yingwei Wang, Lijuan Zhu","doi":"10.26689/par.v6i6.4536","DOIUrl":"https://doi.org/10.26689/par.v6i6.4536","url":null,"abstract":"Objective: To explore the influence of painless childbirth technique on the psychology of primipara. Methods: From July 2020 to June 2022, 108 parturients who received analgesic during delivery in Shaanxi Provincial People’s Hospital were selected as the research subjects (painless delivery group), and 92 parturients who gave birth naturally during the same period were selected as controls (natural delivery group). Psychological tests were performed on the patients. Results: The depression and anxiety scores of parturients in the natural delivery group were significantly higher than those in the painless delivery group (P < 0.05); 9.0% of the patients had coexisting anxiety and depression. Conclusion: The application of painless delivery techniques in obstetrics can improve the negative emotions experienced by primipara, improve their self-efficacy, and relieve their psychological pressure.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45431803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenoid Basal Carcinoma of the Cervix with Squamous Differentiation: A Case Report and Literature Review","authors":"Jianhua Hao, Yafei Zhao, Jinmei Li, Bi-Yun Zhou, Jirui Sun, Qiushuang Ma","doi":"10.26689/par.v6i6.4494","DOIUrl":"https://doi.org/10.26689/par.v6i6.4494","url":null,"abstract":"Adenoid basal carcinoma (ABC) of the cervix is a rare and low-incidence low-grade cervical cancer. In our practice, we encountered a case of cervical ABC with squamous differentiation and high-grade squamous intraepithelial lesion (HSIL) with gland involvement in the peripheral cervix. Reviewing relevant literature and analyzing its clinical manifestations, pathological morphology, and immunohistochemical characteristics would help deepen the understanding of this malignant tumor, so as to make a comprehensive diagnosis with differential diagnosis and prevent misdiagnosis.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44740911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Epigenetic Mechanism and Treatment of Huntington’s Disease","authors":"Yi Shen, Yu Tang","doi":"10.26689/par.v6i6.4512","DOIUrl":"https://doi.org/10.26689/par.v6i6.4512","url":null,"abstract":"Huntington’s disease (HD) is an irreversible neurodegenerative disorder that is inherited in an autosomal dominant manner. In HD, many regions of the human brain are affected, including the striatum, thalamus, and cortex. The mechanism is by the expansion of CAG repeats, which encode glutamine (Q) in the Huntingtin gene on chromosome 4p16.3. Patients with more CAG repeats tend to have a younger age of onset and a higher risk. Mutant HTT protein, translated from mtHtt, would congregate or interact with other proteins, causing damage to the human body. Patients with HD show symptoms like chorea, which is an involuntary motor disability, cognitive deterioration, and psychiatric disturbances. Except for the genetic pathology of HD, the epigenetic mechanism of this disease has made a lot of progress in recent years. This paper primarily focuses on the alternation of deoxyribonucleic acid (DNA) methylation, histone modification, and non-coding ribonucleic acids (ncRNAs) in HD as well as the advancements of epigenetic therapy and healthcare in HD.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44906427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage CCL22 Secretion Under Hypoxic Conditions Promotes the Metastasis of Triple-Negative Breast Cancer","authors":"Wenming Zhao, Bi-Yun Zhou, Jirui Sun, Haizhi Qiao, Jinmei Li, Qiushuang Ma, Xu Wang, Jinku Zhang","doi":"10.26689/par.v6i6.4493","DOIUrl":"https://doi.org/10.26689/par.v6i6.4493","url":null,"abstract":"Objective: To explore the mechanism by which macrophages secrete CCL22 to promote the metastasis of triple-negative breast cancer under hypoxic conditions. Methods: 20 ng/mL mass concentration of phorbol 12-myristate 13-acetate (PMA) cell culture medium, 4?,6-diamidino-2-phenylindole (DAPI), dimethyl sulfoxide (DMSO), trypsin digestion solution, CCL18 Kit , Interleukin (IL)-10 Kit, CCL17 Kit, CCL22 Kit, TRIzolTM Reagent Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Kit, triple-negative breast cancer cells MDA-MB-231 and BT-549, as well as other reagents were used to culture triple-negative breast cancer (TNBC) cells MDA-MB-231 and BT-549 as well as human mononuclear cells THP-1, analyze and observe the metastasis of triple-negative breast cancer cells to the lungs, the secretion of CCL22, the migration of triple-negative breast cancer, and the situation of CCR4. Results: Compared with normal tumor-associated macrophage (TAM), hypoxic TAM further promoted the migration of tumor cells. The number of tumor metastases in the lungs, induced by hypoxic TAM, was significantly higher than that of normoxic TAM. Hypoxia can significantly stimulate the expression of CCL22. CCL22 can significantly promote the migration of MDA-MB-231 and BT-549 cells. The expressions of CCR3, CCR4, and CCR5 in tumor tissues were significantly increased compared with normal tissues, in which CCR4 showed the most significant increase. Conclusion: TAM cultured under hypoxia significantly enhanced the migration ability of TNBC cells and promoted the metastasis of cancer cells to the lungs in vivo. The hypoxic condition induced TAM to secrete CCL22; the expression of CCL22 receptor, CCR4, in breast cancer tissues was significantly higher than that in normal tissues.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46897261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Rat Bladder Cancer","authors":"Jinmei Li, R. Cheng, Bi-Yun Zhou, Zhiqiang Zhang, Q. Zhai, Jinku Zhang","doi":"10.26689/par.v6i6.4495","DOIUrl":"https://doi.org/10.26689/par.v6i6.4495","url":null,"abstract":"Objective: To analyze the effect of bone marrow mesenchymal stem cell therapy on rats with bladder cancer and provide a feasible direction for the treatment of human bladder cancer. Methods: An animal model was constructed, and Model 1 was used as an example. Two groups of rats were injected with anti-upconversion nanoparticles (UCNPs) (experimental group) and 0.9% normal saline (control group), respectively. In vivo imaging was performed to determine the accuracy of the anti-UCNPs method. Results: There were 15 rats in the experimental group with obvious bladder swelling. Among them, 11 rats had cauliflower-like and partially brown bladder tumors, whereas the other four rats had hard, nodular-like protrusions, with indistinct borders and adhesions to the anterior wall of the rectum. Small papillary masses were observed in two rats, local mucosal thickening without tumor formation was observed in two rats, and bladder stones were observed in six rats. The bladder specimens of 15 rats in the control group were pink and shiny, without any tumors. Fourteen rats in the experimental group and 12 rats in the control group had bladder cancer lesions, accounting for 93.33% and 80%, respectively. The detection accuracy of the experimental group was significantly better than that of the control group. Conclusion: Multimodal nanoprobes targeting bladder cancer stem cells in vivo were used to image the orthotopic tumor and lymph node metastasis models of animals by anti-UCNPs imaging to observe the distribution, migration, and differentiation process of bladder cancer stem cells in model mice. It is clear that rare earth upconversion luminescent nanomaterials, modified by BCMab1 and CD44 monoclonal antibodies, can be used as probes for the detection of bladder cancer, the tracking of lymph node metastasis in bladder cancer, and the comprehensive evaluation of the overall efficacy of nanoprobe-targeted therapy for bladder cancer stem cells.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42084989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KIF3C Promotes the Malignant Progression of Lung Cancer Cells A549","authors":"Haiwang Liu, Ran Liu, Meiling Hao, Xing Zhao, Chunhui Li","doi":"10.26689/par.v6i6.4431","DOIUrl":"https://doi.org/10.26689/par.v6i6.4431","url":null,"abstract":"Objective: To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway. Methods: CCK-8 and transwell assays were used to detect the changes in cell proliferation and cell migration ability after being transfected with siKIF3C, as well as the protein expression levels of PI3K, p-PI3K, AKT, and p-AKT following the downregulation of KIF3C by Western blot. Results: The CCK-8 assay showed that the proliferation/viability of lung cancer cells A549 significantly reduced after being transfected with siKIF3C gene (P < 0.05); the migration ability of lung cancer cells A549 was significantly reduced after transfected with siKIF3C gene (P < 0.05); the levels of p-PI3K and p-AKT proteins were downregulated after KIF3C protein knockdown (P < 0.05); however, the detection of PI3K and AKT protein levels was not statistically significant. Conclusion: KIF3C may promote the proliferation and migration ability of lung cancer cells A549 through PI3K/AKT signaling pathway.","PeriodicalId":61025,"journal":{"name":"抗癌研究","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44910732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}