KIF3C Promotes the Malignant Progression of Lung Cancer Cells A549

Abstract

Objective: To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway. Methods: CCK-8 and transwell assays were used to detect the changes in cell proliferation and cell migration ability after being transfected with siKIF3C, as well as the protein expression levels of PI3K, p-PI3K, AKT, and p-AKT following the downregulation of KIF3C by Western blot. Results: The CCK-8 assay showed that the proliferation/viability of lung cancer cells A549 significantly reduced after being transfected with siKIF3C gene (P < 0.05); the migration ability of lung cancer cells A549 was significantly reduced after transfected with siKIF3C gene (P < 0.05); the levels of p-PI3K and p-AKT proteins were downregulated after KIF3C protein knockdown (P < 0.05); however, the detection of PI3K and AKT protein levels was not statistically significant. Conclusion: KIF3C may promote the proliferation and migration ability of lung cancer cells A549 through PI3K/AKT signaling pathway.