Food Science and Biotechnology最新文献_第7页

Establishment and characterization of hTERT-immortalized porcine muscle stem cells, and their prospective uses

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-11 DOI: 10.1007/s10068-024-01785-9

Witsanu Srila, Amorn Pangjantuk, Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa

{"title":"Establishment and characterization of hTERT-immortalized porcine muscle stem cells, and their prospective uses","authors":"Witsanu Srila, Amorn Pangjantuk, Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa","doi":"10.1007/s10068-024-01785-9","DOIUrl":"10.1007/s10068-024-01785-9","url":null,"abstract":"<div><p>Myogenic differentiation ability made porcine muscle satellite cells (MSCs) promising candidates for cultured meat production. While in vitro, porcine primary cells lose differentiation capacity, have short lifespans, and change phenotype. For immortal porcine MSCs, human telomerase reverse transcriptase (hTERT) gene was overexpressed in parental cells to restore telomerase activity and lengthen cell longevity. After selection, G418-resistant cells were expanded and passed by different generations. The hTERT-immortalized MSCs presented spindle-like shape, telomere extension, and indefinite proliferation. In comparison to parental cells, immortal cells grew more rapidly and doubled faster. Immortal MSCs expressed muscle-specific protein and gene markers, were self-renewing stem cells, and could develop into myofibers in vitro. In culture plates with more than 100 generations, immortal MSCs formed tumors, but not lower passaged cells. Today, we showed that hTERT can immortalize primary porcine MSCs and preserve their stem cell characteristics. For research and cultured meat technologies, immortality may be valuable.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 and Processing Breakthroughs","pages":"1597 - 1604"},"PeriodicalIF":2.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-targeted metabolomics revealed that the release mechanism of Tween-80 interferes with prodigiosin synthesis and release by Serratia marcescens SDSPY-136

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-11 DOI: 10.1007/s10068-024-01750-6

Junqing Wang, Tingting Zhang, Yang Liu, Shanshan Wang, Shuhua Liu, Yanlei Han, Hui Xu

{"title":"Non-targeted metabolomics revealed that the release mechanism of Tween-80 interferes with prodigiosin synthesis and release by Serratia marcescens SDSPY-136","authors":"Junqing Wang, Tingting Zhang, Yang Liu, Shanshan Wang, Shuhua Liu, Yanlei Han, Hui Xu","doi":"10.1007/s10068-024-01750-6","DOIUrl":"10.1007/s10068-024-01750-6","url":null,"abstract":"<div><p><i>Serratia marcescens</i> produce prodigiosin as a high-value intracellular secondary metabolite. However, the effect of Tween-80 on prodigiosin release remains unclear. This study aimed to elucidate the physiological characteristics and metabolic mechanisms of <i>S. marcescens</i> in the presence of Tween-80. The results showed that Tween-80 significantly affected prodigiosin's synthesis and extracellular release. Significant changes in cell morphology and zeta (ζ) potential were observed during cell growth. In addition, 245 differential metabolites were identified by non-targeted metabolomics analysis, indicating that Tween-80 affects the metabolism of fatty acids, amino acids, extra-long-chain fatty acids, and their derivatives. Enrichment pathway analysis revealed significant enrichment of lipid metabolism, amino acid metabolism, and other pathways. Finally, in conjunction with the metabolomics results, a prodigiosin yield was achieved at 6.5 g/L in 5 L bioreactors using fed-batch fermentation. The results of this study have significant implications for the regulation of prodigiosin synthesis and metabolism.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 and Processing","pages":"1171 - 1182"},"PeriodicalIF":2.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible fetal bovine serum-reduced boost cultivation of DF-1 by supplementation with plant peptones and yeast extracts

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-09 DOI: 10.1007/s10068-024-01774-y

Juah Chung, Bumjin Joo, Yu Kyong Woo, Jihyun Kim, Young-oh Lee, Seokwon Lim, Choongjin Ban

{"title":"Possible fetal bovine serum-reduced boost cultivation of DF-1 by supplementation with plant peptones and yeast extracts","authors":"Juah Chung, Bumjin Joo, Yu Kyong Woo, Jihyun Kim, Young-oh Lee, Seokwon Lim, Choongjin Ban","doi":"10.1007/s10068-024-01774-y","DOIUrl":"10.1007/s10068-024-01774-y","url":null,"abstract":"<div><p>To reduce the fetal bovine serum (FBS) and enhance the DF-1 proliferation, three plant peptones and three yeast extracts were incorporated into culture medium. The concentration of FBS in the medium varied from 0 to 10%, and the enhanced cell viability was evaluated within the predetermined range for supplementation. In each specific concentration range, the viability was enhanced by the supplementation compared to the control group. In particular, the medium containing 6.25 mg/mL wheat peptone and 1.25% FBS demonstrated a significant increase in viability. This beneficial effect can be attributed to the incorporation of suitable free amino acids (FAAs), peptides, carbohydrates (excluding free sugars), or trace elements through supplementation. Regarding FAAs, the enhanced cell proliferation by glutamate, glutamine, alanine, and non-essential FAAs was contingent upon the specific type of supplementation. The proliferation was suppressed by serine, histidine, threonine, arginine, tyrosine, valine, methionine, phenylalanine, isoleucine, leucine, essential FAAs, and particularly lysine.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 2","pages":"391 - 402"},"PeriodicalIF":2.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm formation, slime production, and antibiotic susceptibility properties of the Bacillus cereus group isolated from fresh vegetables in the Republic of Korea

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-09 DOI: 10.1007/s10068-024-01771-1

Jae-Hyun Yoon, Hana Song, Sun-Young Lee

{"title":"Biofilm formation, slime production, and antibiotic susceptibility properties of the Bacillus cereus group isolated from fresh vegetables in the Republic of Korea","authors":"Jae-Hyun Yoon, Hana Song, Sun-Young Lee","doi":"10.1007/s10068-024-01771-1","DOIUrl":"10.1007/s10068-024-01771-1","url":null,"abstract":"<div><p>This study aimed to characterize the ability of the <i>Bacillus cereus</i> group isolated from conventionally, organically or pesticide-freely grown vegetables to form biofilms with regard to cell surface hydrophobicity (CSH), slime production, and antibiotic susceptibility. Cellular properties (biofilm formation, CSH, and slime production) were measured using an in vitro microplate assay with crystal violet staining, adhesion to hydrocarbons assay, and Congo red broth method, respectively. Consequently, 16, 16, and 16 <i>B</i>. <i>cereus</i> strains were isolated from conventionally, organically, and pesticide-freely grown vegetables, respectively, and 16 (33%) <i>B</i>. <i>cereus</i> isolates were highly biofilm-positive producers. CSH values dramatically varied, ranging from 19 to 74%, among the <i>B</i>. <i>cereus</i> isolates. Additionally, 9, 8, and 8 <i>B</i>. <i>cereus</i> strains isolated from conventionally, organically, and pesticide-freely grown vegetables, respectively, were identified to be slime-positive producers. According to the disc diffusion method, 19 and 41 <i>B</i>. <i>cereus</i> isolates were highly resistant to ampicillin and penicillin, respectively.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 6","pages":"1525 - 1531"},"PeriodicalIF":2.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of blanching condition for removing potassium from spinach using response surface methodology

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-09 DOI: 10.1007/s10068-024-01782-y

Ji-Eun Lim, Sang-Jin Ye, Jae-Sung Shin, Hui-Yun Kim, Ji-Eun Bae, Seon-Min Oh, Moo-Yeol Baik

引用次数: 0

Green technological interventions for controlling carbon footprint in agro-food processing: a critical review

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-07 DOI: 10.1007/s10068-024-01732-8

Shambhavi Singh, Manish Tiwari, Komal Chauhan, Anupama Singh, Harinder Singh Oberoi, Neetu Kumra Taneja, Garima Singh

{"title":"Green technological interventions for controlling carbon footprint in agro-food processing: a critical review","authors":"Shambhavi Singh, Manish Tiwari, Komal Chauhan, Anupama Singh, Harinder Singh Oberoi, Neetu Kumra Taneja, Garima Singh","doi":"10.1007/s10068-024-01732-8","DOIUrl":"10.1007/s10068-024-01732-8","url":null,"abstract":"<div><p>The major cause of climate change has been attributed to the food systems. Thus, sustainability in the agri-food processing industry is becoming increasingly crucial in terms of carbon footprint estimation. The unit operations in the food supply chain, such as processing, packaging, transportation, and consumption, emit various greenhouse gases, which increase the footprint during the food supply chain. Hence, the review article highlighted green technological interventions in the food supply chain with case studies of pre-harvesting and post-harvesting operations. Additional information about carbon footprint (CFP) labeling, packaging, storage, and transportation is discussed to minimize greenhouse gas emissions (GHGE) and enhance consumer awareness in terms of food choices based on the carbon footprint values of the product. Green technologies subject to the food supply chain positively influence sustainability. This technology will aid in the strategic decision-making process for reducing food waste and reducing carbon footprint production.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 6","pages":"1261 - 1283"},"PeriodicalIF":2.4,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction, analysis and antioxidant activity of palm peduncle polysaccharide

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-05 DOI: 10.1007/s10068-024-01762-2

Shiyang Zhou, Wenbin Wang, Jun Tan

引用次数: 0

Effects of epigallocatechin gallate on microbial communities of rainbow trout fillets during ice storage and identification of biogenic amines-producing bacteria

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-04 DOI: 10.1007/s10068-024-01763-1

Nan Zhao, Songmin Zhao, Jinrui Duan, Wensheng Qin, Yingchang Li

{"title":"Effects of epigallocatechin gallate on microbial communities of rainbow trout fillets during ice storage and identification of biogenic amines-producing bacteria","authors":"Nan Zhao, Songmin Zhao, Jinrui Duan, Wensheng Qin, Yingchang Li","doi":"10.1007/s10068-024-01763-1","DOIUrl":"10.1007/s10068-024-01763-1","url":null,"abstract":"<div><p>Biogenic amines-producing bacteria were isolated from rainbow trout fillets and their biogenic amines production ability was investigated. The effects of epigallocatechin gallate (EGCG) on the microbial communities of rainbow trout fillets were also investigated. Fifteen (15) strains of biogenic amines-producing bacteria were isolated from rainbow trout fillets. <i>Pseudomonas psychrophila</i> and <i>Shewanella baltica</i> produced putrescine (73.97 mg/mL) and histamine (48.66 mg/mL), respectively. The high-throughput sequencing results indicated <i>Proteobacteria</i> were the dominant phyla of the both control group and the EGCG treated groups. At the genus level, <i>Shewanella</i> spp. and <i>Pseudomonas</i> spp. were the dominating bacteria in the control group, while <i>Pseudomonas</i> spp. were the dominant bacteria in the EGCG treated groups. EGCG can obviously decrease bacterial diversity of rainbow trout fillets, which was mainly reflected in the relative abundance of <i>Shewanella</i> spp. This study provides microbial insights into controlling the quality of rainbow trout fillets during ice storage.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 6","pages":"1413 - 1421"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of sanitation strategies against Listeria monocytogenes on food-contact surfaces in enoki mushroom (Flammulina velutipes) processing facilities

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-04 DOI: 10.1007/s10068-024-01751-5

Kyung Min Park, Su-Bin Lee, Hyobeen Chae, Injun Hwang, Se-Ri Kim, Hyun Dong Lee, Song-Yi Choi

{"title":"Comparative evaluation of sanitation strategies against Listeria monocytogenes on food-contact surfaces in enoki mushroom (Flammulina velutipes) processing facilities","authors":"Kyung Min Park, Su-Bin Lee, Hyobeen Chae, Injun Hwang, Se-Ri Kim, Hyun Dong Lee, Song-Yi Choi","doi":"10.1007/s10068-024-01751-5","DOIUrl":"10.1007/s10068-024-01751-5","url":null,"abstract":"<div><p>We investigated strategies to reduce <i>Listeria monocytogenes</i> contamination on food contact surfaces during enoki mushroom processing. Chemical disinfectants and thermal treatments were evaluated on conveyor belts, stainless steel, plastic surfaces, and Velcro strips. Without organic matter, chemical disinfectants effectively reduced <i>L. monocytogenes</i>, with stainless steel showing the highest susceptibility. Organic matter decreased disinfectant efficacy, but sodium hypochlorite remained most effective on stainless steel. Peracetic acid was more effective on conveyor belts and plastic surfaces than on stainless steel. Combining peracetic acid with dry heating synergistically reduced <i>L. monocytogenes</i> on Velcro strips. Moist heat at 70 °C alone was insufficient, but when combined with hot air drying, it effectively reduced <i>L. monocytogenes</i> on Velcro strips. Our findings emphasize the importance of surface-specific strategies combining chemical disinfection and thermal treatment for eliminating <i>L. monocytogenes</i> in mushroom processing environments.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 6","pages":"1507 - 1516"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavor patterns of beer fermented by Lachancea thermotolerans

IF 2.4 3区农林科学

Food Science and Biotechnology Pub Date : 2024-12-04 DOI: 10.1007/s10068-024-01769-9

Jeong-Ah Yoon, Seong-Wook Cho, Se-Young Kwun, Eun-Hee Park, Myoung-Dong Kim

引用次数: 0