Food Science and Biotechnology最新文献_第6页

Survival and stress responses of Listeria monocytogenes and Pseudomonas aeruginosa in single- and dual-species biofilms on stainless steel under freeze–thaw conditions 冻融条件下不锈钢单、双种生物膜中单核增生李斯特菌和铜绿假单胞菌的生存和胁迫反应

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-19 DOI: 10.1007/s10068-026-02203-y

Yoon-Mi Ji, Se-Wook Oh

{"title":"Survival and stress responses of Listeria monocytogenes and Pseudomonas aeruginosa in single- and dual-species biofilms on stainless steel under freeze–thaw conditions","authors":"Yoon-Mi Ji, Se-Wook Oh","doi":"10.1007/s10068-026-02203-y","DOIUrl":"10.1007/s10068-026-02203-y","url":null,"abstract":"<div><p>This study investigated the effects of freeze–thaw treatment on survival and stress responses in single-species biofilms of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> and their dual-species biofilm, designated as LM, PA, and LP, respectively. Biofilms were formed on stainless steel and subjected to different freezing and thawing conditions. Smaller freeze–thaw temperature shifts resulted in higher viable cell counts of LM and PA, reaching 4.79 ± 0.22 and 7.67 ± 0.17 log CFU/cm<sup>2</sup>, respectively. In contrast, LM, which showed the lowest extracellular polymeric substances (EPS) production, had the lowest viable count under the largest temperature shift (4.01 ± 0.17 log CFU/cm<sup>2</sup>). This survival pattern was accompanied by greater membrane damage and membrane potential changes under larger temperature shifts. LP produced 9.7- and 2.4-fold higher EPS component levels than LM and PA, respectively, and maintained structural stability and <i>L. monocytogenes</i> viability, suggesting potential protection by interspecies interactions and the biofilm matrix.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2715 - 2725"},"PeriodicalIF":3.9,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane-based fractionation of red beetroot juice: bioactive compound enrichment and sugar removal 红甜菜根汁的膜分离：生物活性化合物的富集和糖的去除

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-19 DOI: 10.1007/s10068-026-02213-w

Aslı Arslan Kulcan

引用次数: 0

Characterization of the potential antihypertensive activity of Aster pseudoglehnii leaf extracts 紫菀叶提取物潜在降压活性的表征

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-16 DOI: 10.1007/s10068-026-02211-y

Yu Ri Kim, Joong-Hyuck Auh

{"title":"Characterization of the potential antihypertensive activity of Aster pseudoglehnii leaf extracts","authors":"Yu Ri Kim, Joong-Hyuck Auh","doi":"10.1007/s10068-026-02211-y","DOIUrl":"10.1007/s10068-026-02211-y","url":null,"abstract":"<div><p>Chronic hypertension is closely associated with oxidative stress and endothelial dysfunction, both of which contribute to cardiovascular disease development. This study evaluated the potential antihypertensive and vasoprotective effects of <i>Aster pseudoglehnii</i> leaf extract and identified its major bioactive metabolites. Dried leaves were extracted with 70% ethanol and fractionated into two subfractions (F1 and F2) using preparative liquid chromatography. Antioxidant capacity was evaluated by measuring total phenolic and flavonoid contents, DPPH radical-scavenging activity, and oxygen radical absorbance capacity, while angiotensin-converting enzyme (ACE) inhibitory activity was assessed to determine antihypertensive potential. F2 exhibited stronger antioxidant and ACE inhibitory activities than F1. In EA.hy926 endothelial cells, F2 suppressed inflammatory adhesion molecules (ICAM-1 and VCAM-1) and enhanced endothelial nitric oxide synthase (eNOS) activation through phosphorylation. Untargeted metabolomic analysis revealed enrichment of cynarine, 4,5-dicaffeoylquinic acid, kaempferol-3-<i>O</i>-rutinoside, and lysophosphatidylglycerol (16:0) in F2. These findings indicate that F2 exerts potential antihypertensive effects through coordinated antioxidant and endothelial-protective mechanisms.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2627 - 2637"},"PeriodicalIF":3.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02211-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of species-specific SYBR green-based real-time PCR assay to differentiate between Lupinus angustifolius and Lupinus albus 基于SYBR绿色的实时荧光定量PCR方法的建立以区分红豆狼疮和白狼疮

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-16 DOI: 10.1007/s10068-026-02207-8

Ayoung Cho, Ji Seok Yi, Yun Seo Jung, Ho Soo Lim, Moon Ik Chang

引用次数: 0

Detection of tea adulterants using Fourier transform near-infrared (FT-NIR) spectroscopy combined with chemometrics 傅里叶变换近红外光谱与化学计量学相结合检测茶叶掺假

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-12 DOI: 10.1007/s10068-026-02205-w

Nelum Priyadarshani Piyasena, Savani Ulpathakumbura, Nishantha Edirisinghe, Mahasen Ranatunga

{"title":"Detection of tea adulterants using Fourier transform near-infrared (FT-NIR) spectroscopy combined with chemometrics","authors":"Nelum Priyadarshani Piyasena, Savani Ulpathakumbura, Nishantha Edirisinghe, Mahasen Ranatunga","doi":"10.1007/s10068-026-02205-w","DOIUrl":"10.1007/s10068-026-02205-w","url":null,"abstract":"<div><p>Food adulteration is a major global concern due to its economic implications and potential health risks. This study aimed to develop and validate a rapid and reliable method to quantify sugar adulteration in black tea representing the major tea-growing regions of Sri Lanka, as well as to qualitatively identify maltodextrin adulteration using Fourier transform near-infrared spectroscopy (FT-NIR). Partial least squares regression models were established for sugar quantification, while high-performance liquid chromatography was used as the reference method for quantitative sugar analysis and qualitative detection of maltodextrin. The developed models demostrated strong predictive performance, with coefficients of determination of 0.89 for fructose, 0.90 for sucrose, and 0.93 for glucose, and residual predictive deviation values exceeding 2.5 for sugars. Furthermore, principal component analysis clearly differentiated maltodextrin-adulterated tea from pure samples, explaining 89% of the total variance, demonstrating the suitability of FT-NIR spectroscopy for rapid adulteration detection in black tea.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2517 - 2525"},"PeriodicalIF":3.9,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced aroma compound production by new Saccharomyces cerevisiae isolates for bread and rice hydrolysate medium fermentations 面包和大米水解培养基发酵新分离的酿酒酵母增强香气化合物生产

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-10 DOI: 10.1007/s10068-026-02201-0

Chan-Haeng Lee, Jeong-Ah Yoon, Min-Kyung Ju, Dae-Joong Kim, Myoung-Dong Kim

{"title":"Enhanced aroma compound production by new Saccharomyces cerevisiae isolates for bread and rice hydrolysate medium fermentations","authors":"Chan-Haeng Lee, Jeong-Ah Yoon, Min-Kyung Ju, Dae-Joong Kim, Myoung-Dong Kim","doi":"10.1007/s10068-026-02201-0","DOIUrl":"10.1007/s10068-026-02201-0","url":null,"abstract":"<div><p><i>Saccharomyces cerevisiae</i>, also known as brewer’s yeast, is widely used in food and beverage fermentations. In this study, newly isolated <i>S. cerevisiae</i> strains, NIYL33997 and NIYL33999, were evaluated for fermentation performance in bread and rice hydrolysate medium compared to commercial yeasts. In bread fermentation, NIYL33999 produced higher levels of phenylethyl alcohol and ethyl acetate, corresponding to 2.1- and 3.0-fold increases over Saf-instant yeast. NIYL33997 showed the highest ethyl octanoate production. During fermentation in rice hydrolysate medium, NIYL33999 produced the highest concentrations of aroma compounds. Specifically, isoamyl alcohol, phenylethyl alcohol, and ethyl acetate levels increased by approximately 1.2-, 1.9-, and 1.3-fold, respectively, compared with those produced by the Songcheon yeast. NIYL33997 exhibited similar increases with lower ester production. Principal component analysis revealed clear differentiation of aroma patterns among the strains. In conclusion, <i>S. cerevisiae</i> NIYL33997 and NIYL33999 exhibit distinct aroma profiles, with NIYL33999 demonstrating potential as a starter for fermented foods.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2581 - 2592"},"PeriodicalIF":3.9,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivity assessment and metabolic profiling of Phellinus gilvus. A pathway to novel therapeutics 茶树的生物活性评价及代谢谱分析。通往新疗法的途径

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-08 DOI: 10.1007/s10068-026-02199-5

Mehmoona Sharif, Umme Habiba Saeeda, Aneela Nawaz, Muhammad Farman, Malik Badshah, Aamer Ali Shah, Muniba Jadoon, Lolakhon Ettibaeva, Samiullah Khan

{"title":"Bioactivity assessment and metabolic profiling of Phellinus gilvus. A pathway to novel therapeutics","authors":"Mehmoona Sharif, Umme Habiba Saeeda, Aneela Nawaz, Muhammad Farman, Malik Badshah, Aamer Ali Shah, Muniba Jadoon, Lolakhon Ettibaeva, Samiullah Khan","doi":"10.1007/s10068-026-02199-5","DOIUrl":"10.1007/s10068-026-02199-5","url":null,"abstract":"<div><p><i>Phellinus gilvus</i> has potent antioxidant and antimicrobial activities, but the exact bioactive components are unknown. The study investigated <i>P. gilvus</i> and unveiled six novel compounds as its principal antimicrobial agents. Hexane fraction exhibited antibacterial activity against <i>S. aureus</i> (19 ± 0.57 mm) and <i>B. subtilis</i> (17 ± 0.57 mm) at a minimum inhibitory concentration of 31.25 µg/mL. The ethyl acetate fraction showed antifungal activity with inhibitory zones of 21 ± 1.52 mm against <i>C. albicans</i>, 15 ± 1.00 mm against <i>A. niger,</i> and 17 ± 1.00 mm against <i>A. flavus</i>. Most extracts possessed antioxidant potential with a maximum percent of 78 ± 1.00% by the ethyl acetate fraction. Cytotoxic and hemolytic activity was within an acceptable range and confirmed the safety profile for the fractions. The metabolic profile revealed polysaccharides, quinones, and triterpenes, emphasizing the potential of <i>P. gilvus</i> as a promising source for developing novel antifungal and antibacterial agents.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2613 - 2626"},"PeriodicalIF":3.9,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148613283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the impact of microalgae powder on physicochemical and functional properties of hard candies 微藻粉对硬糖理化及功能特性的影响

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-03 DOI: 10.1007/s10068-026-02174-0

Shafia Maryam, Berkay Berk, Hilmi Eriklioglu, Baris Ege Gulenc, Kosain Kousar, Mecit Halil Oztop, Muhammad Qasim Hayat, Sarper Dogdu, Mehmet Ali Marangoz, Hussnain Ahmed Janjua

{"title":"Evaluating the impact of microalgae powder on physicochemical and functional properties of hard candies","authors":"Shafia Maryam, Berkay Berk, Hilmi Eriklioglu, Baris Ege Gulenc, Kosain Kousar, Mecit Halil Oztop, Muhammad Qasim Hayat, Sarper Dogdu, Mehmet Ali Marangoz, Hussnain Ahmed Janjua","doi":"10.1007/s10068-026-02174-0","DOIUrl":"10.1007/s10068-026-02174-0","url":null,"abstract":"<div><p>This study investigated the feasibility of incorporating microalgae powders into model hard candies and evaluated their effects on physicochemical, structural, and functional properties. Microalgae species including <i>Chlorella vulgaris</i>, <i>Spirulina platensis</i>, <i>Dictyosphaerium</i> sp., and <i>Pectinodesmus</i> sp. were incorporated into candy formulations. Scanning electron microscopy revealed a semi-smooth candy surface with algal particles embedded within the amorphous sugar matrix. Time-domain nuclear magnetic resonance and hardness analysis indicated reduced crystallization in algae-enriched candies. Fourier-transform infrared spectroscopy identified characteristic polysaccharide bands along with amide and protein-associated functional groups derived from algal biomass. Biochemical analyses confirmed the presence of antioxidant activity, phenolic compounds, and flavonoids. Shelf-life evaluation demonstrated the retention of structural and textural stability over three months of storage. Microalgae incorporation influenced candy color, crystalline structure, moisture content, and nutritional composition while enhancing antioxidant functionality. This study provides a basis for the development of microalgae-fortified functional confectionery products.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 8","pages":"2191 - 2205"},"PeriodicalIF":3.9,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02174-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of high-purity Aeromonas salmonicida extracellular vesicles and their inhibitory activity against Pseudomonas fluorescens biofilm formation 高纯度沙门氏气单胞菌胞外囊泡的鉴定及其对荧光假单胞菌生物膜形成的抑制活性

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-02 DOI: 10.1007/s10068-026-02188-8

Zhenzhen Ning, Wei Rao, Liangting Shao, Yang Dong, Silu Liu, Xinqi Yang, Xinglian Xu, Huhu Wang

{"title":"Characterization of high-purity Aeromonas salmonicida extracellular vesicles and their inhibitory activity against Pseudomonas fluorescens biofilm formation","authors":"Zhenzhen Ning, Wei Rao, Liangting Shao, Yang Dong, Silu Liu, Xinqi Yang, Xinglian Xu, Huhu Wang","doi":"10.1007/s10068-026-02188-8","DOIUrl":"10.1007/s10068-026-02188-8","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) have emerged as important mediators of bacterial interspecies interactions and may play critical roles in food spoilage dynamics, but co-isolated contaminants hinder their accurate characterization. To address this, we compared conventional ultracentrifugation (UC) and two combined methods for isolating A<i>eromonas salmonicida</i> EVs (ASEVs). The ultrafiltration–UC–density gradient centrifugation (FCD) method achieved a 6.9-fold purity increase over UC. Comparative proteomics revealed that contaminants mainly originated from cytoplasm, and flagellum-, pili-, and phage-associated proteins were identified as purity markers. The ASEVs proteins are primarily located in membrane structures and mainly implicated in metabolic and membrane transport pathways. Functional assays demonstrated that ASEVs inhibited planktonic growth of <i>Pseudomonas fluorescens</i> and reduced biofilm formation by 40.48%. This study establishes a theoretical foundation for elucidating the characteristics of food spoilage bacteria EVs and their roles in bacterial interspecies interactions.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 8","pages":"2283 - 2297"},"PeriodicalIF":3.9,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactivities, sources, and biosynthesis of 1-deoxynojirimycin: a bibliometric‑informed review 1-脱氧诺吉霉素的生物活性、来源和生物合成：文献计量学综述

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-05-30 DOI: 10.1007/s10068-026-02180-2

Nairu Ji, Fei Ren, Qiaolei Zhu, Bingyu Zhao, Yunping Zhu

{"title":"Bioactivities, sources, and biosynthesis of 1-deoxynojirimycin: a bibliometric‑informed review","authors":"Nairu Ji, Fei Ren, Qiaolei Zhu, Bingyu Zhao, Yunping Zhu","doi":"10.1007/s10068-026-02180-2","DOIUrl":"10.1007/s10068-026-02180-2","url":null,"abstract":"<div><p>1-Deoxynojirimycin (DNJ) is a potent α-glucosidase inhibitor with considerable potential for functional foods. However, its application is constrained by low natural abundance and limited sources. Through a visualized bibliometric analysis of DNJ research (1994–2023) using Citespace and VOSviewer, this review reveals a distinct evolutionary trajectory: early efforts concentered on source discovery and bioactivity validation whereas the current frontier has shifted toward biosynthesis and yield enhancement, particularly via microbial engineering. Building on these insights, the multifaceted bioactivities of DNJ were systematically discussed. A critical emphasis on comparing biosynthetic pathways in plants and microbes is confirmed by evaluating the DNJ sources and extraction methods. Microbial fermentation is identified as the most scalable platform for sustainable production. In addition, the applications in DNJ-fortified foods are discussed. Future perspectives highlight that integrating metabolic engineering with fermentation optimization is key to overcoming the production bottlenecks, guiding the next phase of DNJ research and development.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 11","pages":"3091 - 3111"},"PeriodicalIF":3.9,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148859787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0