Food Science and Biotechnology最新文献_第5页

Screening of wild-type Saccharomyces cerevisiae strains for single-cell protein production in white grape juice 白葡萄汁生产单细胞蛋白的酿酒酵母菌野生型筛选

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-30 DOI: 10.1007/s10068-026-02215-8

İpek Ceren Yeşildağ, Remziye Yılmaz

{"title":"Screening of wild-type Saccharomyces cerevisiae strains for single-cell protein production in white grape juice","authors":"İpek Ceren Yeşildağ, Remziye Yılmaz","doi":"10.1007/s10068-026-02215-8","DOIUrl":"10.1007/s10068-026-02215-8","url":null,"abstract":"<div><p>Increasing demand for sustainable protein sources has intensified interest in single-cell protein (SCP) production. This study evaluated the fermentation performance of wild-type <i>Saccharomyces cerevisiae</i> strains isolated from grape vineyards to identify candidates for SCP bioprocesses. Following morphological characterization and pH screening, strains were cultivated in white grape juice supplemented with 1% (w/v) yeast extract for 72 h at 28 °C. K-means clustering, principal component analysis (PCA), and growth curve modelling were applied to differentiate the strains. Strains HUF16M2K10004 and HUF16M3G11088 reached wet cell weights of 21.56 ± 0.13 and 27.90 ± 0.49 g/L, dry cell weights of 6.01 ± 0.05 and 6.46 ± 0.44 g/L, water-soluble protein contents of 2.00 ± 0.15 and 1.92 ± 0.07 g/L, with growth rates of 0.15 ± 0.02 and 0.23 ± 0.02 h⁻<sup>1</sup>, comparable to those of the commercial reference strains. Both wild-type strains represent preliminary candidates for future SCP-oriented characterization studies.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2603 - 2612"},"PeriodicalIF":3.9,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02215-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth-coupled CRISPRi screening reveals YBR013C as a novel genetic target for secretion of an ovalbumin-based fusion protein in Saccharomyces cerevisiae 生长偶联CRISPRi筛选显示，YBR013C是酿酒酵母分泌卵清蛋白融合蛋白的新基因靶点

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-30 DOI: 10.1007/s10068-026-02231-8

Woohyuk Lee, Da In Yun, Sun-Ki Kim

{"title":"Growth-coupled CRISPRi screening reveals YBR013C as a novel genetic target for secretion of an ovalbumin-based fusion protein in Saccharomyces cerevisiae","authors":"Woohyuk Lee, Da In Yun, Sun-Ki Kim","doi":"10.1007/s10068-026-02231-8","DOIUrl":"10.1007/s10068-026-02231-8","url":null,"abstract":"<div><p>Ovalbumin (OVA) is an important egg white protein that is widely used in food applications owing to its functional properties. Although precision fermentation offers a sustainable alternative for OVA production, limited extracellular secretion remains a significant challenge. To address this gap, this study aimed to develop a growth-coupled screening strategy for identifying a novel gene that enhances OVA secretion in <i>Saccharomyces cerevisiae</i>. To achieve this, an OVA–β-glucosidase (GH1) fusion protein was used to link secretion efficiency to cellobiose-dependent growth. A genome-wide CRISPR interference (CRISPRi) library was introduced into an OVA–GH1–expressing strain and screened under isolated conditions. Repression of <i>YBR013C</i>, a gene of unknown function, consistently resulted in superior growth. Reintroduction of the <i>YBR013C</i>-targeting CRISPRi plasmid reproduced this phenotype and increased extracellular OVA–GH1 levels. This study identifies <i>YBR013C</i> as a novel factor associated with protein secretion and presents a generalizable screening platform for improving protein secretion in yeast.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 10","pages":"2971 - 2977"},"PeriodicalIF":3.9,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Density functional theory and machine learning approaches for deciphering the antifungal properties of aldehydes against Penicillium italicum resistant to prochloraz 密度泛函理论和机器学习方法用于解读醛类药物对抗丙氯嗪的意大利青霉的抗真菌特性

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-29 DOI: 10.1007/s10068-026-02217-6

Chunjing Wang, Xingyu Liu, Xiumei Chen, Jinxin Che, Nengguo Tao, Fei Pan

{"title":"Density functional theory and machine learning approaches for deciphering the antifungal properties of aldehydes against Penicillium italicum resistant to prochloraz","authors":"Chunjing Wang, Xingyu Liu, Xiumei Chen, Jinxin Che, Nengguo Tao, Fei Pan","doi":"10.1007/s10068-026-02217-6","DOIUrl":"10.1007/s10068-026-02217-6","url":null,"abstract":"<div><p>Experiments were conducted to evaluate the antifungal activity of chain aldehydes with potent antifungal properties against <i>Penicillium italicum</i> (<i>P. italicum</i>) resistant to prochloraz. The results showed that <i>trans,trans</i>-2,4-nonadienal and <i>trans,trans</i>-2,4-octadienal possessed the strong antifungal activity with MIC values of 0.10 μL/mL for both compounds. Density functional theory (DFT) revealed that compounds with highly delocalized electron systems exhibited significantly stronger antifungal potency. Machine learning (ML) techniques were used to build regression models for identifying the influential structure features of aldehydes, and the random forest (RF) model had lower error levels. We can conclude that the DFT coupled with ML is a reliable, fast and inexpensive tool for solving problems related to the structure properties and antifungal activity. The strategy can be extended to find safe and effective novel inhibitors of <i>P. italicum</i> resistant to prochloraz for the development of green inhibitors of postharvest diseases in fruits and vegetables.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 9","pages":"2691 - 2702"},"PeriodicalIF":3.9,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148614909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation 来自太子参的环肽异茶碱减轻神经炎症：一项整合分子动力学、炎症途径分析和体内验证的研究

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-27 DOI: 10.1007/s10068-026-02223-8

Bo Li, Xueying Shi, Erhua Chen, Xianwen Chen

{"title":"Cyclopeptide heterophyllin from Pseudostellaria heterophylla alleviates neuroinflammation: a study integrating molecular dynamics, inflammatory pathway analysis, and in vivo validation","authors":"Bo Li, Xueying Shi, Erhua Chen, Xianwen Chen","doi":"10.1007/s10068-026-02223-8","DOIUrl":"10.1007/s10068-026-02223-8","url":null,"abstract":"<div><p>This study evaluated the ameliorative effects of Heterophyllin J, a cyclic peptide derived from <i>Pseudostellaria heterophylla</i>, on lipopolysaccharide (LPS)-induced neuroinflammation in mice. Biochemical, and molecular analyses revealed that Heterophyllin J dose-dependently reduced hippocampal pro-inflammatory cytokines (IFN-γ, IL-1β, IL-6, TNF-α), suppressed oxidative stress (restoring glutathione and lowering malondialdehyde), and alleviated iron accumulation. Western blotting demonstrated Heterophyllin J downregulated NLRP3, Caspase1, and TLR4/NF-κB pathway proteins (COX-2, iNOS, Myd88) while enhancing antioxidant markers (Nrf2, HO-1, NQO1) via Keap1/Nrf2 activation. Heterophyllin J also modulated JAK/STAT signaling, restoring JAK1/2 phosphorylation and reducing STAT1/3 activation, thereby attenuating apoptosis (lower Bax, cleaved caspase-3; higher Bcl-2) and ferroptosis (upregulating GPX4, PCBP1, SLC40A1). These findings highlight Heterophyllin J’s multimodal mechanism: regulating TLR4-driven inflammation, rebalancing iron metabolism, and activating antioxidant pathways to counteract neuroinflammation. The results position Heterophyllin J as a promising therapeutic candidate for neurodegenerative diseases linked to neuroinflammatory and oxidative stress pathways.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 10","pages":"2979 - 2993"},"PeriodicalIF":3.9,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of polyphenol-rich foods on glyoxal and methylglyoxal during simulated gastrointestinal digestion 模拟胃肠消化过程中富含多酚的食物对乙二醛和甲基乙二醛的影响

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-27 DOI: 10.1007/s10068-026-02193-x

Esma Oğuz, Mustafa Yaman

引用次数: 0

Upcycling sesame meal by-product into diverse food matrices: physicochemical properties and antioxidant profiling 将芝麻粕副产品升级为各种食品基质：理化性质和抗氧化分析

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-23 DOI: 10.1007/s10068-026-02220-x

Jiwon Go, Youngjae Shin

{"title":"Upcycling sesame meal by-product into diverse food matrices: physicochemical properties and antioxidant profiling","authors":"Jiwon Go, Youngjae Shin","doi":"10.1007/s10068-026-02220-x","DOIUrl":"10.1007/s10068-026-02220-x","url":null,"abstract":"<div><p>Sesame meal, a by-product of sesame oil extraction, is rich in protein, dietary fiber, and antioxidant lignans, but remains underutilized. This study evaluated its applicability in structurally distinct food matrices, including energy bars, tofu, and acorn jelly, to explore its potential for sustainable functional food development. Physicochemical properties (color, soluble solid content, and pH), total flavonoids, total phenolics, individual polyphenols, and antioxidant activities were analyzed. The energy bar exhibited the highest soluble solid content, whereas tofu showed the highest pH. The energy bar exhibited the highest total flavonoid (41.92 mg catechin equivalents /100 g) and phenolic content (93.51 mg gallic acid equivalents /100 g), whereas acorn jelly showed the strongest radical scavenging activity. Distinct polyphenol profiles were observed among the products, indicating matrix-dependent differences in antioxidant activity. These findings confirm the feasibility of upcycling sesame meal into diverse food systems and highlight its potential as a sustainable, value-added functional ingredient.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 10","pages":"2851 - 2859"},"PeriodicalIF":3.9,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring and exposure assessment of total and inorganic arsenic in health functional foods distributed in Korea 韩国销售的保健功能食品中总砷和无机砷的监测与暴露评估

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-22 DOI: 10.1007/s10068-026-02219-4

Dahui Kim, Ku‑won Kim, Juyoung Lee, Shinai Choi, Choong-In Yun, Young-Jun Kim

引用次数: 0

Monitoring and risk assessment of biogenic amines in seafood commonly consumed in Korea 韩国常见海产品中生物胺的监测与风险评估

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-22 DOI: 10.1007/s10068-026-02218-5

WanTing Zhao, Siwoo Kim, MiKyeong Song, BoKyung Moon

引用次数: 0

Fermentation-associated compositional changes in hot-brewed Chenopodium formosanum leaf infusion under defined microbial conditions 在特定的微生物条件下，热酿台湾藜叶浸渍中发酵相关的成分变化

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-22 DOI: 10.1007/s10068-026-02221-w

Pei-Yin Liao, Wei-Yong Lin, Yun-Hsuan Hsu, Wen-Zheng Huang, Chih-Min Yang

{"title":"Fermentation-associated compositional changes in hot-brewed Chenopodium formosanum leaf infusion under defined microbial conditions","authors":"Pei-Yin Liao, Wei-Yong Lin, Yun-Hsuan Hsu, Wen-Zheng Huang, Chih-Min Yang","doi":"10.1007/s10068-026-02221-w","DOIUrl":"10.1007/s10068-026-02221-w","url":null,"abstract":"<div><p><i>Chenopodium formosanum</i> leaves are an underutilized agricultural by-product rich in phenolic-related constituents. This study evaluated fermentation dynamics and compositional changes of brewed <i>C. formosanum</i> leaf infusion (CFLI) during defined kombucha-type fermentation. An infusion prepared at 90 °C for 20 min was selected based on phenolic-related parameters and antioxidant activity. Fermentation with yeasts, acetic acid bacteria, and lactic acid bacteria showed stage-dependent microbial changes, characterized by early proliferation, an active middle transition, and later population decline. These changes were accompanied by acidification, sucrose hydrolysis, organic acid accumulation, and increases in total phenolic content, total flavonoid content, rutin, and DPPH radical-scavenging activity. In an exploratory <i>Drosophila melanogaster</i> assay, CFLI and day 6 fermented samples showed changes in climbing performance and antioxidant enzyme activities; however, these responses cannot be attributed specifically to fermentation-derived effects because no sucrose-supplemented, matrix-matched unfermented control was included. These findings support CFLI as a substrate for defined fermented beverage systems.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 10","pages":"2959 - 2970"},"PeriodicalIF":3.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02221-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DPP-4 inhibitory and insulin-signaling-modulating potential of Allomyrina dichotoma larval protein hydrolysate 异米蝇幼虫蛋白水解物的DPP-4抑制和胰岛素信号调节潜力

IF 3.9 3区农林科学

Food Science and Biotechnology Pub Date : 2026-06-22 DOI: 10.1007/s10068-026-02216-7

Jae Hee Han, Joong-Hyuck Auh

{"title":"DPP-4 inhibitory and insulin-signaling-modulating potential of Allomyrina dichotoma larval protein hydrolysate","authors":"Jae Hee Han, Joong-Hyuck Auh","doi":"10.1007/s10068-026-02216-7","DOIUrl":"10.1007/s10068-026-02216-7","url":null,"abstract":"<div><p>This study investigated the DPP-4 inhibitory and insulin signaling modulating activities of <i>Allomyrina dichotoma</i> (rhinoceros beetle) larval protein hydrolysate. Following enzymatic hydrolysis, three fractions were obtained by HPLC fractionation. Among them, fraction 3 (F3) exhibited the strongest dipeptidyl peptidase-4 (DPP-4) inhibitory activity (IC<sub>50</sub>: 0.72 mg/mL). In an insulin-resistant HepG2 cell model, F3 significantly increased glucose uptake and enhanced Akt phosphorylation at Thr308, along with upregulation of GLUT4 expression, indicating improved insulin signaling responses. Furthermore, LC–MS/MS analysis identified 22 compounds enriched in hydrophobic and aromatic amino acids within F3. Molecular docking analysis predicted that these compounds interact with key DPP-4 active-site residues, including Glu205/Glu206, Tyr547, and Tyr662. Additionally, quantitative analysis revealed substantial levels of Trp, Val-Leu, and Met-Leu, which have previously been reported as DPP-4 inhibitory compounds. Collectively, these findings suggest that <i>A. dichotoma</i> larval hydrolysate contains bioactive peptides with DPP-4-inhibitory and insulin-signaling-modulating properties, supporting its potential for glucose homeostasis.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 12","pages":"3465 - 3477"},"PeriodicalIF":3.9,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02216-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0