Chloroplast DNA markers for distinguishing seaweeds Ulva prolifera and Capsosiphon fulvescens using real-time quantitative PCR and their application in commercial products

IF 2.4 3区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Food Science and Biotechnology Pub Date : 2025-01-19 DOI:10.1007/s10068-024-01804-9

Ui Cheol Park, Sung Je Choi, Ju Hee Kim, Cheol Seong Jang

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Abstract

Ulva prolifera and Capsosiphon fulvescens, popular seaweed foods in Korea, get frequently mixed in commercial food products because of their overlapping growing locations, seasons, and morphological characteristics. In this study, molecular markers were developed to distinguish these species using a real-time PCR. Six primer pairs were designed for chloroplast genes cemA, psbB, and rbcL. R² values of all primer pairs were > 0.99, and slopes ranged between − 3.14 and − 3.45, and the efficiency was 94.89–108.39%. Blind test results for 20 samples to verify the applicability of the developed primer pairs perfectly match the blind mixture data. Of the 14 tested commercial products, four C. fulvescens products had mixing of a small quantity of U. prolifera species. The developed real-time quantitative PCR assay may aid in the quality control of commercial products of both species as well as the protection of consumer rights.

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来源期刊

Food Science and Biotechnology FOOD SCIENCE & TECHNOLOGY-

CiteScore

5.40

自引率

3.40%

发文量

174

审稿时长

2.3 months

期刊介绍： The FSB journal covers food chemistry and analysis for compositional and physiological activity changes, food hygiene and toxicology, food microbiology and biotechnology, and food engineering involved in during and after food processing through physical, chemical, and biological ways. Consumer perception and sensory evaluation on processed foods are accepted only when they are relevant to the laboratory research work. As a general rule, manuscripts dealing with analysis and efficacy of extracts from natural resources prior to the processing or without any related food processing may not be considered within the scope of the journal. The FSB journal does not deal with only local interest and a lack of significant scientific merit. The main scope of our journal is seeking for human health and wellness through constructive works and new findings in food science and biotechnology field.