Food Science and Biotechnology最新文献_第3页

Multifunctional probiotic properties of Heyndrickxia coagulans RS24: from in vitro characterization to in vivo colitis amelioration Heyndrickxia凝固菌RS24的多功能益生菌特性：从体外表征到体内结肠炎改善

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-03-04 DOI: 10.1007/s10068-026-02110-2

Hyun-Ho Lee, Ye-Jin Kim, Beom-Sik Choi, Youngju Song, Dae-Ok Kim, Hee Kang, Se-Won Jang, Joo-Hyun Oh, Chan-Sung Park, Yong-Wook Lee, Hee-Do Kim, Cheon-Seok Park

{"title":"Multifunctional probiotic properties of Heyndrickxia coagulans RS24: from in vitro characterization to in vivo colitis amelioration","authors":"Hyun-Ho Lee, Ye-Jin Kim, Beom-Sik Choi, Youngju Song, Dae-Ok Kim, Hee Kang, Se-Won Jang, Joo-Hyun Oh, Chan-Sung Park, Yong-Wook Lee, Hee-Do Kim, Cheon-Seok Park","doi":"10.1007/s10068-026-02110-2","DOIUrl":"10.1007/s10068-026-02110-2","url":null,"abstract":"<div><p><i>Heyndrickxia coagulans</i> RS24, isolated from traditional Korean fermented foods, was identified as a promising probiotic candidate through comprehensive in vitro and in vivo evaluations. RS24 demonstrated stability under simulated gastrointestinal conditions and compliance with safety standards for probiotics. Functionally, RS24 showed moderate antioxidant activity, significant immunostimulatory effects in RAW 264.7 macrophages, and strong adhesion to intestinal epithelial cells. In a DSS-induced colitis mouse model, RS24 effectively attenuated disease severity by reducing weight loss, preserving colon length, and reducing histological inflammation. It also enhanced ex vivo Th1 (IFN-γ) and Th2 (IL-4) cytokine responses without disrupting systemic immune cell populations, indicating a balanced immunomodulatory profile. Collectively, these results demonstrate that <i>H. coagulans</i> RS24 possesses a favorable safety profile and multiple probiotic functionalities, including antioxidant, immunomodulatory, and gut-protective activities. Its strain-specific efficacy highlights RS24 as a strong candidate for development as a next-generation probiotic targeting intestinal inflammation and host immune modulation.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1247 - 1258"},"PeriodicalIF":3.1,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of simultaneous and sequential irradiation with pulsed ultraviolet, visible, and near-infrared light on microbial inactivation 脉冲紫外、可见光和近红外光同时和连续照射对微生物灭活的影响

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-03-03 DOI: 10.1007/s10068-026-02118-8

Ji-Yoon Lee, Jung-Hee Ryu, Hye-Jae Choi, Hee-Jeong Hwang, Myong-Soo Chung

{"title":"Effects of simultaneous and sequential irradiation with pulsed ultraviolet, visible, and near-infrared light on microbial inactivation","authors":"Ji-Yoon Lee, Jung-Hee Ryu, Hye-Jae Choi, Hee-Jeong Hwang, Myong-Soo Chung","doi":"10.1007/s10068-026-02118-8","DOIUrl":"10.1007/s10068-026-02118-8","url":null,"abstract":"<div><p>The bactericidal efficacy of simultaneous and sequential irradiation with ultraviolet (UV), visible (VIS), and near-infrared (NIR) light was evaluated using an intense pulsed light (IPL) device equipped with optical bandpass filters. <i>Salmonella enterica</i> serovar Enteritidis, <i>Staphylococcus aureus</i>, and <i>Saccharomyces cerevisiae</i> were subjected to three treatment modes: simultaneous UV + VIS + NIR, sequential UV followed by VIS + NIR [UV-(VIS + NIR)], and sequential VIS + NIR followed by UV [(VIS + NIR)-UV]. Simultaneous irradiation achieved ≥ 5 log reductions at lower energy doses than both sequential irradiation modes. Between sequential treatments, (VIS + NIR)-UV exhibited faster microbial reduction than UV-(VIS + NIR), highlighting the importance of irradiation sequence. Notably, (VIS + NIR)-UV induced a photoreactivation mechanism that increased the <i>S. aureus</i> population. These results demonstrate that both spectral composition and timing of exposure significantly affect microbial susceptibility to IPL. The findings provide new insights into optimizing wavelength integration and sequencing strategies for effective nonthermal microbial inactivation in food safety applications.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 6","pages":"1673 - 1681"},"PeriodicalIF":3.1,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02118-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes of nutritional, digestion and antioxidant characteristics of chickpea during cooking processes 鹰嘴豆在蒸煮过程中营养、消化和抗氧化特性的变化

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-27 DOI: 10.1007/s10068-026-02111-1

Xiaoyan Zhao, Fanghong Wang, Jinghui Feng, Meng Wang, Hongkai Liu, Yunping Zhu

{"title":"Changes of nutritional, digestion and antioxidant characteristics of chickpea during cooking processes","authors":"Xiaoyan Zhao, Fanghong Wang, Jinghui Feng, Meng Wang, Hongkai Liu, Yunping Zhu","doi":"10.1007/s10068-026-02111-1","DOIUrl":"10.1007/s10068-026-02111-1","url":null,"abstract":"<div><p>Based on the literature, cooking can alter the nutritional properties and functionality of food. So five representative cooking methods (boiling, steaming, roasting, stir-frying and deep-frying) were selected to process the chickpea. This study aimed to elucidate how different cooking influenced nutrient composition, protein structure, and digestibility in chickpea. Compared to raw, the ash and protein contents of cooked chickpea decreased, while an obvious increase in total carbohydrate and iodine blue value was observed in five cooking methods. Deep-frying kept more fat, iodine blue value, total starch, total carbohydrate and total energy. After chickpea of cooking treatment, the protein digestibility and antioxidant activity improved, the starch digestion rate decreased. FTIR spectra also showed that the cooking method could affect the secondary structures in chickpea, such as the steaming had the highest random coil conformation. Accordingly, the experimental results can provide a valuable reference for availing chickpea through appropriate cooking methods.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1159 - 1170"},"PeriodicalIF":3.1,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metal accumulation and fecal microbiota response in black soldier fly larvae: identification of Leminorella grimontii as a potential indicator species 黑兵蝇幼虫重金属积累和粪便菌群反应：格林蒙小微球作为潜在指示种的鉴定

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-25 DOI: 10.1007/s10068-026-02114-y

Junho Yu, Hana Baek, Varun Jaiswal, Miey Park, Hae-Jeung Lee

{"title":"Heavy metal accumulation and fecal microbiota response in black soldier fly larvae: identification of Leminorella grimontii as a potential indicator species","authors":"Junho Yu, Hana Baek, Varun Jaiswal, Miey Park, Hae-Jeung Lee","doi":"10.1007/s10068-026-02114-y","DOIUrl":"10.1007/s10068-026-02114-y","url":null,"abstract":"<div><p>With growing environmental concerns about marine pollution from heavy metals, black soldier fly larvae (BSFL) are gaining attention for their potential use in sustainable waste-to-feed systems. In this study, BSFL were raised on diets containing cadmium (Cd), lead (Pb), mercury (Hg), and arsenic (As) to assess their growth, metal accumulation patterns, and changes in microbiota. While Cd mainly accumulated in the larval body, Pb, Hg, and As were primarily excreted through exuviae and feces. This pattern remained consistent even when larvae were fed naturally contaminated mackerel heads. Microbiome analysis of fecal samples showed that <i>Leminorella grimontii</i> had a strong positive correlation with Hg and As concentrations, suggesting its potential as a microbial indicator species for heavy metal exposure. These findings support the use of BSFL in bioconversion systems and suggest <i>L. grimontii</i> as a new biomarker for environmental monitoring.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Experimental procedure and fecal microbiome results</p></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1329 - 1338"},"PeriodicalIF":3.1,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147560858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical compositions and bioactivity of essential oils of Citri Reticulata Pericarpium Viride from three provinces in China 中国三省绿皮柑精油化学成分及生物活性研究

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-21 DOI: 10.1007/s10068-026-02112-0

Tian-Xiao Li, Fei-Yang Xiong, Wen Cai, Ya-Ning Fang, Xiao Yang, Jia-Xin Li, Xiao-Ying Ji, Hui Xi, Xue-Wei Jia, Feng-Cheng Shi

{"title":"Chemical compositions and bioactivity of essential oils of Citri Reticulata Pericarpium Viride from three provinces in China","authors":"Tian-Xiao Li, Fei-Yang Xiong, Wen Cai, Ya-Ning Fang, Xiao Yang, Jia-Xin Li, Xiao-Ying Ji, Hui Xi, Xue-Wei Jia, Feng-Cheng Shi","doi":"10.1007/s10068-026-02112-0","DOIUrl":"10.1007/s10068-026-02112-0","url":null,"abstract":"<div><p>This study aimed to reveal the differences in chemical constituents and bioactivity of essential oils (EOs) of Citri Reticulatae Pericarpium Viride (CRPV) from different growth origins in China. EOs of CRPV from Sichuan (EO-SC), Anhui (EO-AH), and Guangdong (EO-GD) provinces were extracted using hydrodistillation. The optimized conditions for hydrodistillation were sample-solvent ratio of 1:10 and extraction time of more than 3.0 h. Olfactory sensory evaluation suggested that EO-AH possessed strong citrus, fruity, and freshness aromas, and EO-GD exhibited stronger spicy and woody aromas. GC–MS analysis indicated that the contents of volatile compounds in EO-AH and EO-GD were obviously higher than that of EO-SC. D-limonene, linalool, o-cymene, and <i>γ</i>-terpinolene, etc. were revealed as the key mutual flavor compounds for three EOs. The strong citrus, fruity, and freshness aromas of EO-AH could be associated with high ROAV values of D-limonene, linalool, and <i>γ</i>-terpinolene. EO-AH and EO-GD exhibited strong antioxidant and antibacterial activities.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1147 - 1157"},"PeriodicalIF":3.1,"publicationDate":"2026-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147560501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of rosemary and ginger extracts on DNA integrity, oxidative stress, and vascular endothelial function: a pilot randomized, double-blind, placebo-controlled trial 迷迭香和生姜提取物对DNA完整性、氧化应激和血管内皮功能的保护作用：一项随机、双盲、安慰剂对照试验

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-19 DOI: 10.1007/s10068-026-02113-z

Min Ju Park, Jina Hong, Sewon Jeong, Ji Yeon Kim

{"title":"Protective effects of rosemary and ginger extracts on DNA integrity, oxidative stress, and vascular endothelial function: a pilot randomized, double-blind, placebo-controlled trial","authors":"Min Ju Park, Jina Hong, Sewon Jeong, Ji Yeon Kim","doi":"10.1007/s10068-026-02113-z","DOIUrl":"10.1007/s10068-026-02113-z","url":null,"abstract":"<div><p>Chronic oxidative stress driven by unhealthy lifestyles contributes to cumulative cellular damage. Rosemary and ginger contain polyphenols reported to modulate cellular stress responses, including oxidative stress; however, evidence for their combined effects in humans is limited. This pilot randomized, double-blind, placebo-controlled trial investigated the effects of combined rosemary and ginger extract on oxidative stress, DNA damage, inflammation, and vascular endothelial signaling in healthy adults. Seventy-two adults were classified as Healthy or Risk, with the Risk group characterized by overweight and smoking. Participants received placebo, low-dose, or high-dose extract for 4 weeks. Supplementation did not significantly alter circulating oxidative stress or inflammatory markers. In the Risk group, oxidative DNA damage significantly decreased in both dose groups, whereas erythrocyte phosphorylated endothelial nitric oxide synthase expression increased in the high-dose group. Short-term supplementation was associated with protection against oxidative DNA damage and modulation of endothelial signaling in the risk group, warranting longer-term studies.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1307 - 1318"},"PeriodicalIF":3.1,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147560412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm formation, cell hydrophobicity, cytotoxic potential, genetic diversity, resistance to antimicrobials, and toxigenic profile properties among Bacillus cereus groups isolated from kitchen sponges in the Republic of Korea 从韩国厨房海绵中分离的蜡样芽孢杆菌群的生物膜形成、细胞疏水性、细胞毒性潜力、遗传多样性、抗菌剂耐药性和产毒谱特性

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-19 DOI: 10.1007/s10068-026-02095-y

Se-Gyeong Yoon, Sang-Woo Lee, Hae-In Lee, Hyun-Dong Cho, Jae-Hyun Yoon

{"title":"Biofilm formation, cell hydrophobicity, cytotoxic potential, genetic diversity, resistance to antimicrobials, and toxigenic profile properties among Bacillus cereus groups isolated from kitchen sponges in the Republic of Korea","authors":"Se-Gyeong Yoon, Sang-Woo Lee, Hae-In Lee, Hyun-Dong Cho, Jae-Hyun Yoon","doi":"10.1007/s10068-026-02095-y","DOIUrl":"10.1007/s10068-026-02095-y","url":null,"abstract":"<div><p>This study aimed to characterize <i>Bacillus cereus</i> isolates recovered from the kitchen sponges used in domestic environments, the Republic of Korea, with a particular focus on analyzing antibiotic susceptibility, biofilm formation, cell surface hydrophobicity (CSH), cytotoxic potential, genetic diversity, and toxigenic profile properties. <i>B</i>. <i>cereus</i> isolates displayed moderate or weak biofilm-forming capabilities, and CSH levels greatly differed among the isolates. Several isolates displayed strong cytotoxic effects despite lacking key toxin-encoding genes. All 26 <i>B</i>. <i>cereus</i> isolates were clustered into eight distinct groups and survived at ClO<sub>2</sub>, NaClO, ethanol, acetic acid, and lactic acid, while being highly sensitive to H<sub>2</sub>O<sub>2</sub>. Additionally, 7 (26.9%) and 11 (42.3%) <i>B</i>. <i>cereus</i> isolates possessed enterotoxin genes and the cereulide-encoding gene <i>ces</i>, respectively. Our findings highlight kitchen sponges as potential reservoirs of disinfectant/sanitizer-tolerant and toxigenic <i>B</i>. <i>cereus</i> strains, underscoring a cross contamination risk in domestic environments, emphasizing the need for optimized hygiene management strategies.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1339 - 1353"},"PeriodicalIF":3.1,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-026-02095-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147560406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schizonepeta tenuifolia extract improves muscle strength in aged female mice via anti-inflammatory effects 荆芥提取物通过抗炎作用提高老年雌性小鼠的肌肉力量

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-19 DOI: 10.1007/s10068-026-02106-y

Young Sook Kim, Heung Joo Yuk, Dong-Seon Kim

{"title":"Schizonepeta tenuifolia extract improves muscle strength in aged female mice via anti-inflammatory effects","authors":"Young Sook Kim, Heung Joo Yuk, Dong-Seon Kim","doi":"10.1007/s10068-026-02106-y","DOIUrl":"10.1007/s10068-026-02106-y","url":null,"abstract":"<div><p>Sarcopenia remains as incurable condition, characterized by the progressive muscle atrophy, which is particularly prevalent in the elderly and individuals with restricted mobility. Functional foods play a role in preventing muscle loss and maintaining muscle function. Here, we evaluated the first beneficial effect of <i>Schizonepeta tenuifolia</i> flower stalk ethanolic extract (DKB138) on sarcopenia in aged mice and in vitro. UPLC-Q-Tof/MS analysis identified seven components in DKB138. In C2C12 skeletal muscle cells, DKB138 inhibited hydrogen peroxide-induced cell death. In a mouse model, nine-week and 18-month-old C57BL/6J female mice were treated with DKB138 (200 mg/kg and 300 mg/kg) or OMJ (positive control, 300 mg/kg). The DKB138-treated groups exhibited significantly enhanced grip strength, reduced inflammation, and lower serum LDL levels. In conclusion, the present study demonstrated that DKB138 inhibits muscle degradation, reduces inflammation, and improves muscle strength, making it a promising functional food for combating muscle dysfunction in the elderly.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 6","pages":"1577 - 1587"},"PeriodicalIF":3.1,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave irradiation induced free radical reactions on the acetaldehyde formation in model wine solutions: process optimization and mechanistic insights 微波辐射诱导的自由基反应对模型酒溶液中乙醛的形成：过程优化和机理的见解

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-14 DOI: 10.1007/s10068-026-02107-x

Xiao-Wen Yang, Jiang-Feng Yuan, Wen-Ting Duan, Zhi-Jun Qiu, Yi-Fan He, Kun Si

{"title":"Microwave irradiation induced free radical reactions on the acetaldehyde formation in model wine solutions: process optimization and mechanistic insights","authors":"Xiao-Wen Yang, Jiang-Feng Yuan, Wen-Ting Duan, Zhi-Jun Qiu, Yi-Fan He, Kun Si","doi":"10.1007/s10068-026-02107-x","DOIUrl":"10.1007/s10068-026-02107-x","url":null,"abstract":"<div><p>This study investigated the mechanisms of acetaldehyde generation in model wine solutions under microwave irradiation. Electron paramagnetic resonance spectroscopy identified 1-hydroxyethyl radicals as a primary initiator for acetaldehyde under microwave irradiation. Response surface methodology optimized microwave parameters (600 W, 7.5 min, 34 °C), achieving the maximum acetaldehyde yield of 0.973 mg/L. Ferrous ion and ethanol enhanced radical chain propagation. With the ethanol concentration was increased from 3 to 96%, acetaldehyde formation rose from 0.29 to 2.89 mg/L. Meanwhile, as the concentration of ferrous ions increased, the acetaldehyde concentration gradually increased from 0.43 mg/L to 1.1 mg/L. In contrast, mannitol exhibited dose-dependent inhibition, when its concentration was raised from 5 to 80 g/L, acetaldehyde formation progressively decreased from 0.43 to 0.01 mg/L. These findings established microwave-induced 1-hydroxyethyl radicals as the dominant factor for accelerating acetaldehyde accumulation and pigment formation, providing a physicochemical foundation for microwave applications in wine processing.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1191 - 1203"},"PeriodicalIF":3.1,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147559223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of an isomaltooligosaccharide utilizing α-glucosidase from the resistant-starch degrading gut bacterium Ruminococcoides bili FMB-J6 利用抗淀粉降解肠道细菌瘤胃球菌FMB-J6的α-葡萄糖苷酶鉴定低聚异麦芽糖

IF 3.1 3区农林科学

Food Science and Biotechnology Pub Date : 2026-02-13 DOI: 10.1007/s10068-026-02109-9

Jae-Won Lee, Ye-Jin Kim, Dong-Ho Seo, Seong-Min Choi, Jong-Hyun Jung, Cheon-Seok Park

{"title":"Identification and characterization of an isomaltooligosaccharide utilizing α-glucosidase from the resistant-starch degrading gut bacterium Ruminococcoides bili FMB-J6","authors":"Jae-Won Lee, Ye-Jin Kim, Dong-Ho Seo, Seong-Min Choi, Jong-Hyun Jung, Cheon-Seok Park","doi":"10.1007/s10068-026-02109-9","DOIUrl":"10.1007/s10068-026-02109-9","url":null,"abstract":"<div><p><i>Ruminococcoides</i> is a genus abundant in the human colon and plays a key role in carbohydrate metabolism. These microorganisms can utilize a variety of substrates for growth in the gut, but their metabolic characteristics toward dietary sugars remain poorly characterized. In our previous study, we isolated <i>Ruminococcoides bili</i> FMB-J6 from human feces and identified a unique glucosidase with the potential to degrade prebiotic substrates. This enzyme belongs to the GH13_44 subfamily and exhibits high hydrolytic activity toward ingested sugars, including sucrose and isomaltooligosaccharides. Structural comparisons based on the predicted model revealed that this enzyme possesses a more open substrate-binding pocket. As a result, it shows elevated activity toward substrates containing both α-1,4 and α-1,6 linkages, with a preference for α-1,6-linkages. These findings highlight the potential of this enzyme as a novel α-glucosidase with broad substrate specificity, enabling the utilization of prebiotic compounds in <i>Ruminococcoides.</i></p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"35 5","pages":"1237 - 1245"},"PeriodicalIF":3.1,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147559243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0