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Fermented mixed grain ameliorates chronic stress-induced depression-like behavior and memory deficit 发酵混合谷物改善慢性应激诱导的抑郁样行为和记忆缺陷
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-27 DOI: 10.1007/s10068-023-01387-x
Jae Gwang Song, Bomi Lee, Do Eon Kim, Bong Kyeong Seo, Nam Su Oh, Sae Hun Kim, Hyung Wook Kim
{"title":"Fermented mixed grain ameliorates chronic stress-induced depression-like behavior and memory deficit","authors":"Jae Gwang Song,&nbsp;Bomi Lee,&nbsp;Do Eon Kim,&nbsp;Bong Kyeong Seo,&nbsp;Nam Su Oh,&nbsp;Sae Hun Kim,&nbsp;Hyung Wook Kim","doi":"10.1007/s10068-023-01387-x","DOIUrl":"10.1007/s10068-023-01387-x","url":null,"abstract":"<div><p>Fermented mixed grain (FG) has beneficial anti-cancer, antioxidant, and anti-inflammatory effects. In this study, we investigated the effects of FG on gut inflammation, brain dysfunction, and anxiety/depression-like behavior induced by unpredictable chronic mild stress (UCMS) in mice. Mice were administered mixed grain or FG for 3 weeks and were then exposed to UCMS for 4 weeks. FG administration ameliorated stress-induced anxiety/despair-like behavior. FG administration also prevented UCMS-induced memory impairment. Additionally, the mRNA levels of 5-HTR1A and IL-6 were restored to normal levels in the brains of FG-administered mice. FG administration also inhibited intestinal damage in stressed mice compared with that in the UCMS (without FG) group. These results suggest that FG can alleviate stress-induced intestinal damage, brain dysfunction, and cognitive impairment.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 4","pages":"969 - 979"},"PeriodicalIF":2.4,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41411084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rosa roxburghii Tratt juice inhibits NF-κB and increases IL-2 to alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients 刺梨汁抑制NF-κB,升高IL-2,缓解砷中毒患者外周血foxp3介导的Tregs失衡
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-24 DOI: 10.1007/s10068-023-01384-0
Qi Wang, Xiaolin Fang, Baofei Sun, Kai Zhu, Maolin Yao, Shaofeng Wei, Aihua Zhang
{"title":"Rosa roxburghii Tratt juice inhibits NF-κB and increases IL-2 to alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients","authors":"Qi Wang,&nbsp;Xiaolin Fang,&nbsp;Baofei Sun,&nbsp;Kai Zhu,&nbsp;Maolin Yao,&nbsp;Shaofeng Wei,&nbsp;Aihua Zhang","doi":"10.1007/s10068-023-01384-0","DOIUrl":"10.1007/s10068-023-01384-0","url":null,"abstract":"<div><p>Arsenic can cause immune inflammation, which is the basis of arsenic-induced damage to multiple organs and systems. Forkhead box P3 (Foxp3)-labelled CD4<sup>+</sup>CD25<sup>+</sup> regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis. Nuclear factor-κb (NF-κB) and Interleukin-2 (IL-2) are critical regulators of Foxp3. Rosa roxburghii Tratt (RRT) is an edible medicinal plant with anti-inflammation effects. In this study, a control group (<i>n</i> = 41) and an arseniasis group (<i>n</i> = 209) were recruited, and screened subjects from the arseniasis patients for RRTJ (<i>n</i> = 46) or placebo (<i>n</i> = 43) to explore the possible mechanism by which RRT alleviates immune inflammation. The results indicated that RRTJ can inhibits NF-κB and increases IL-2, and alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients. In summary, these findings suggest a novel intervention or therapeutic target for immune inflammation in arseniasis patients and provide new evidence that RRTJ inhibits immune inflammation.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 4","pages":"935 - 944"},"PeriodicalIF":2.4,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43508717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Paraprobiotics: definition, manufacturing methods, and functionality 益生菌:定义、制造方法和功能
IF 2.9 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-17 DOI: 10.1007/s10068-023-01378-y
Na‑Kyoung Lee, Young-Seo Park, Dae-Kyung Kang, Hyun-Dong Paik
{"title":"Paraprobiotics: definition, manufacturing methods, and functionality","authors":"Na‑Kyoung Lee,&nbsp;Young-Seo Park,&nbsp;Dae-Kyung Kang,&nbsp;Hyun-Dong Paik","doi":"10.1007/s10068-023-01378-y","DOIUrl":"10.1007/s10068-023-01378-y","url":null,"abstract":"<div><p>Probiotics are living microorganisms that are beneficial to the host, enhancing the immune response by promoting antibody production, regulating cytokine secretion, and stimulating T cells. However, probiotics have limitations in that they require viability control and have a short shelf life. Recently, the use of paraprobiotics has gained attention. These include dead bacterial cells, bacterial fractions, and cell lysate that have health benefits and are stable and safe for use. Paraprobiotics comprise molecules of bacterial cell wall compounds, such as peptidoglycans, teichoic acids, polysaccharides, and cell surface proteins. Paraprobiotics are manufactured by a diverse range of techniques, including thermal treatments, high pressure, ultraviolet rays, sonication, ionizing radiation, and pH modification. Their beneficial health effects include immunomodulatory, intestinal balancing, anticancer, and antimicrobial activities. Therefore, this review summarizes and discusses the manufacturing methods and bioavailability of paraprobiotics and suggests their potential health advantages.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"32 14","pages":"1981 - 1991"},"PeriodicalIF":2.9,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42672209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Optimization of vitamin B12 nano-emulsification and encapsulation using spontaneous emulsification 自发乳化法优化维生素B12纳米乳化及包封工艺
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-13 DOI: 10.1007/s10068-023-01357-3
Shabnam Karbalaei-Saleh, Shima Yousefi, Masoud Honarvar
{"title":"Optimization of vitamin B12 nano-emulsification and encapsulation using spontaneous emulsification","authors":"Shabnam Karbalaei-Saleh,&nbsp;Shima Yousefi,&nbsp;Masoud Honarvar","doi":"10.1007/s10068-023-01357-3","DOIUrl":"10.1007/s10068-023-01357-3","url":null,"abstract":"<div><p>In this study, the use of low-energy methods for nanoemulsification of vitamin B12 was investigated to protect this bioactive substance. The effects of sunflower oil concentrations (4–8%), Tween 80 (8–16%), and vitamin B12 (5–15%) on the physicochemical properties of B12 nanoemulsion were evaluated using response surface methodology (RSM). The results indicated that the quadratic model was the most fitting model for experimental data. Optimization revealed that the optimal formulation contained 6.5% sunflower oil, 9.6% Tween 80, and 13% vitamin B12, resulting in maximum efficiency, viscosity, and vitamin B12 content, as well as minimum pH, turbidity, <i>p</i>-Anisidine index, particle size, and polydispersity index (PDI). Under optimal conditions, pH, viscosity, turbidity, efficiency, vitamin B12, p-Anisidine index, PDI, and particle size were 7.24, 17.0024 cp, 2.19, 51.98%, 5.54 ppm, 0.01, 0.34, and 322 nm, respectively. This study highlights the effectiveness of spontaneous emulsification as a carrier for the encapsulation of bioactive compounds.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 2","pages":"399 - 415"},"PeriodicalIF":2.4,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43241280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oral administration of collagen peptide in SKH-1 mice suppress UVB-induced wrinkle and dehydration through MAPK and MAPKK signaling pathways, in vitro and in vivo evidence 体外和体内均有证据表明,口服SKH-1小鼠胶原肽可通过MAPK和MAPKK信号通路抑制uvb诱导的皱纹和脱水
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-03 DOI: 10.1007/s10068-023-01362-6
Cheol Hyeon Cho, Wonchul Lim, Woo-Jin Sim, Tae-Gyu Lim
{"title":"Oral administration of collagen peptide in SKH-1 mice suppress UVB-induced wrinkle and dehydration through MAPK and MAPKK signaling pathways, in vitro and in vivo evidence","authors":"Cheol Hyeon Cho,&nbsp;Wonchul Lim,&nbsp;Woo-Jin Sim,&nbsp;Tae-Gyu Lim","doi":"10.1007/s10068-023-01362-6","DOIUrl":"10.1007/s10068-023-01362-6","url":null,"abstract":"<div><p>Skin aging is induced by exposure to extrinsic factors, causing various diseases and adversely affecting aesthetics. Studies have suggested that as the quality of life improves, demand for beauty and nutritional cosmetics increases. Here, the protective effects of collagen peptide against UV-induced skin damage were evaluated in vitro and in vivo. Collagen peptide inhibited water loss and UVB irradiation-induced HA degradation in the skin of SKH-1 mice. Additionally, collagen peptide dose-dependently inhibited UVB-induced wrinkle formation, epidermal thickness, and elastase activity. These results suggest that collagen peptide regulates collagen degradation through the MAPK and MAPKK pathway. In addition, collagen peptide administration did not affect changes in weight of the liver, spleen, and kidney, or enzymatic indicators of liver damage. Taken together, oral administration of collagen peptide improved the effects of UV-induced skin aging without toxicity. Therefore, this study supports the development of collagen peptide for skin aging prevention in nutricosmetic products.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 4","pages":"955 - 967"},"PeriodicalIF":2.4,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42473240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms 细胞外基质降解酶作为食品相关微生物生物膜控制策略
IF 2.9 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-07-01 DOI: 10.1007/s10068-023-01373-3
Joo-Sung Kim, Min-Cheol Lim, Se-Min Kim, Joo-Young Lee
{"title":"Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms","authors":"Joo-Sung Kim,&nbsp;Min-Cheol Lim,&nbsp;Se-Min Kim,&nbsp;Joo-Young Lee","doi":"10.1007/s10068-023-01373-3","DOIUrl":"10.1007/s10068-023-01373-3","url":null,"abstract":"<div><p>Biofilm is one of the major problems in food industries and is difficult to be removed or prevented by conventional sanitizers. In this review, we discussed the extracellular matrix-degrading enzymes as a strategy to control biofilms of foodborne pathogenic and food-contaminating bacteria. The biofilms can be degraded by using the enzymes targeting proteins, polysaccharides, extracellular DNA, or lipids which mainly constitute the extracellular polymeric substances of biofilms. However, the efficacy of enzymes varies by the growth medium, bacterial species, strains, or counterpart microorganisms due to a high variation in the composition of extracellular polymeric substances. Several studies demonstrated that the combined treatment using conventional sanitizers or multiple enzymes can synergistically enhance the biofilm removal efficacies. In this review, the application of the immobilized enzymes on solid substrates is also discussed as a potential strategy to prevent biofilm formation on food contact surfaces.\u0000</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"32 12","pages":"1745 - 1761"},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41083511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Rottlerin suppresses lipid accumulation by inhibiting de novo lipogenesis and adipogenesis via LRP6/mTOR/SREBP1C in 3T3-L1 adipocytes Rottlerin通过抑制3T3-L1脂肪细胞中的LRP6/mTOR/SREBP1C的新生脂肪生成和脂肪生成来抑制脂质积累
IF 2.9 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-06-30 DOI: 10.1007/s10068-023-01339-5
Yejin Kim, Hyun Kyung Kim, Sumin Kang, Hayoon Kim, Gwang-woong Go
{"title":"Rottlerin suppresses lipid accumulation by inhibiting de novo lipogenesis and adipogenesis via LRP6/mTOR/SREBP1C in 3T3-L1 adipocytes","authors":"Yejin Kim,&nbsp;Hyun Kyung Kim,&nbsp;Sumin Kang,&nbsp;Hayoon Kim,&nbsp;Gwang-woong Go","doi":"10.1007/s10068-023-01339-5","DOIUrl":"10.1007/s10068-023-01339-5","url":null,"abstract":"<div><p>Rottlerin is isolated from <i>Mallotus japonicus</i>, a plant rich in polyphenols. Rottlerin is a selective PKCδ-inhibitor and is also known as an uncoupler of oxidative phosphorylation and anti-neoplastic agent. However, its anti-obesity effect is yet to be established. Therefore, this study tested whether rottlerin inhibits adipogenesis and de novo lipogenesis via the LRP6/mTOR/SREBP1C pathway in 3T3-L1 adipocytes. Rottlerin dramatically decreased lipid accumulation assessed by Oil Red O as evidence to support the cellular phenotype (<i>p</i> &lt; 0.001). Pivotal messenger RNA and protein expressions associated with de novo lipogenesis (SREBP1C, ACC1, FAS, and SCD1) and adipogenesis (PPARγ and C/EBPα) were subsequentially verified by rottlerin in a dose-dependent manner (<i>p</i> &lt; 0.05). Further investigation revealed that rottlerin reduced the AKT/mTOR pathway via diminished total protein of LRP6 (<i>p</i> &lt; 0.05). Collectively, these findings establish a causal link between rottlerin, LRP6, and the altered nutrient-sensing mTOR pathway, in which rottlerin regulates de novo lipogenesis and adipogenesis in white adipocytes.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"32 10","pages":"1445 - 1452"},"PeriodicalIF":2.9,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-023-01339-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5160425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Skin-whitening effects of Spergularia marina by suppressing MITF translocation 抑制MITF易位对白芍皮肤美白的影响
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-06-30 DOI: 10.1007/s10068-023-01376-0
Jae-Hoon Kim, Woo-Jin Sim, Jisoo Nam, Soo-Hyun Park, Ji-Hye Song, Tae Gyu Nam, Jong Hun Kim, Wonchul Lim, Tae-Gyu Lim
{"title":"Skin-whitening effects of Spergularia marina by suppressing MITF translocation","authors":"Jae-Hoon Kim,&nbsp;Woo-Jin Sim,&nbsp;Jisoo Nam,&nbsp;Soo-Hyun Park,&nbsp;Ji-Hye Song,&nbsp;Tae Gyu Nam,&nbsp;Jong Hun Kim,&nbsp;Wonchul Lim,&nbsp;Tae-Gyu Lim","doi":"10.1007/s10068-023-01376-0","DOIUrl":"10.1007/s10068-023-01376-0","url":null,"abstract":"<div><p><i>Spergularia marina</i> is a plant that grows in salty regions along the coastline and exerts radical-scavenging and anti-inflammatory effects. In this study, we investigated the skin-whitening effects of <i>S. marina</i> extract (SME) in B16F10 melanoma cells. SME was found to exert radical-scavenging effects. It suppressed α-melanocyte-stimulating hormone-induced melanogenesis and tyrosinase activity. We also assessed the melanin production signaling pathway to identify the inhibitory action mechanism of SME on melanogenesis. SME decreased the protein expression levels of tyrosinase-related protein (TRP)-1, TRP-2, and tyrosinase, which play important roles in melanogenesis. Furthermore, western blotting revealed that SME inhibited the nuclear translocation of melanocyte inducing transcription factor (MITF), which is a transcription factor for TRP-1, TRP-2, and tyrosinase, suggesting that SME exerts its skin-whitening effect by inhibiting MITF nuclear translocation. Therefore, SME may potentially be used in skin-whitening medicines and cosmetics.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 4","pages":"925 - 933"},"PeriodicalIF":2.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43191332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiplex PCR detection method of genetically modified canola event (MON94100, LBFLFK, and NS-B50027-4) combined with capillary electrophoresis 转基因油菜事件(MON94100、LBFLFK、NS-B50027-4)复合毛细管电泳检测方法
IF 2.4 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-06-30 DOI: 10.1007/s10068-023-01377-z
Seung-Man Suh, Hyun-Jae Kim, Min-Ki Shin, Seung-Jin Hong, Jae-Eun Cha, Hae-Yeong Kim
{"title":"Multiplex PCR detection method of genetically modified canola event (MON94100, LBFLFK, and NS-B50027-4) combined with capillary electrophoresis","authors":"Seung-Man Suh,&nbsp;Hyun-Jae Kim,&nbsp;Min-Ki Shin,&nbsp;Seung-Jin Hong,&nbsp;Jae-Eun Cha,&nbsp;Hae-Yeong Kim","doi":"10.1007/s10068-023-01377-z","DOIUrl":"10.1007/s10068-023-01377-z","url":null,"abstract":"<div><p>Genetically modified organisms (GMOs) have been continuously developed for their convenience and productivity. In the past three years, three new GM canola events (MON94100, LBFLFK, and NS-B50027-4) have been developed. To efficiently control these GM canola events, the detection methods were needed. Therefore, the multiplex PCR method combined with capillary electrophoresis was developed for three GM canola events. Ten GM canola, eighteen GM soybean, thirty-two GM maize, and ten non-GM crops were used to evaluate the specificity of the method. The detection limit of the multiplex PCR assay was determined to be 0.005 ng in the DNA mixture and 0.1% in the spiked sample. The aim of this study was to establish multiplex PCR coupled with capillary electrophoresis for the newly produced three GM canola events. The developed method is expected to contribute to monitor the commercially available GM canola events.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"33 3","pages":"637 - 643"},"PeriodicalIF":2.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42843263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzymatic time−temperature indicator with cysteine-loaded chitosan microspheres/silver nanoparticles 半胱氨酸负载壳聚糖微球/银纳米粒子的酶促时间-温度指示剂
IF 2.9 3区 农林科学
Food Science and Biotechnology Pub Date : 2023-06-26 DOI: 10.1007/s10068-023-01369-z
Hye Won Cho, Dong Un Shin, Sang Won Kim, Eun Seol Kim, Byeong Jae Park, Dong Hwa Kim, Yong Woon Jung, Seung Ju Lee
{"title":"Enzymatic time−temperature indicator with cysteine-loaded chitosan microspheres/silver nanoparticles","authors":"Hye Won Cho,&nbsp;Dong Un Shin,&nbsp;Sang Won Kim,&nbsp;Eun Seol Kim,&nbsp;Byeong Jae Park,&nbsp;Dong Hwa Kim,&nbsp;Yong Woon Jung,&nbsp;Seung Ju Lee","doi":"10.1007/s10068-023-01369-z","DOIUrl":"10.1007/s10068-023-01369-z","url":null,"abstract":"<div><p>A time−temperature indicator (TTI) based on acid−base reaction was developed by applying a new pH dye composed of cysteine-loaded chitosan (Cys-CS) microspheres and silver nanoparticles (AgNPs). It was hypothesized that cysteine released by the disintegration of Cys-CS microspheres at a critical pH would cause AgNPs to aggregate, leading to color change. Cys-CS microspheres were produced as water-in-oil (paraffin oil, MCT oil, soybean oil) emulsions according to the KOH addition method. An enzymatic TTI was made using glucose oxidase, glucose, and catalase. Only paraffin oil produced Cys-CS microspheres (average diameter, 335 ± 100 µm), whereas the others did not, probably due to saponification with KOH. FTIR analysis confirmed that cysteine was encapsulated in the microspheres. The microspheres disintegrated at pH 6.18 in a titration test. The TTI pH gradually decreased and showed a sudden color change at pH 6.10, which was similar to the critical pH of microsphere disintegration.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"32 13","pages":"1873 - 1881"},"PeriodicalIF":2.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10068-023-01369-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41084000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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