{"title":"Effect of rice protein on the retrogradation of waxy rice starch gels.","authors":"Manhee Baek, Semin Jeong, Sujin Jeon, Saehun Mun","doi":"10.1007/s10068-025-01887-y","DOIUrl":"10.1007/s10068-025-01887-y","url":null,"abstract":"<p><p>This study investigated the effects of rice protein (RP) addition on the retrogradation of waxy rice starch (WRS) gels during storage. The retrogradation behavior was evaluated using a rapid visco analyser, a differential scanning calorimeter, an X-ray diffractometer, a texture analyser, and a scanning electron microscope. The incorporation of RP inhibited the swelling of WRS, leading to reductions in trough viscosities, as well as setback values. DSC analysis indicated a reduction in melting enthalpy, while XRD showed decreased peak intensity in retrograded WRS with RP addition. After 3 days of storage at 4 °C, gel hardness increased, but RP significantly mitigated this effect. SEM observations showed that a 5% RP addition produced larger pore sizes and a looser network structure, supporting the inhibition of retrogradation. These findings suggest that RP effectively retards the retrogradation of WRS, offering potential applications in enhancing the texture and shelf-life of starch-based food products.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2507-2518"},"PeriodicalIF":2.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hang Li, Mengyuan Li, Manman Zhang, Ruixue Liu, Nan Li, Yongsheng Wang, Zhihan Wang, Shihan Wang
{"title":"Waste valorization of <i>Rana chensinensis</i> ovum protein isolates versus commercially available proteins isolates from physicochemical, structural, and functional properties to stabilizing Pickering emulsions.","authors":"Hang Li, Mengyuan Li, Manman Zhang, Ruixue Liu, Nan Li, Yongsheng Wang, Zhihan Wang, Shihan Wang","doi":"10.1007/s10068-025-01870-7","DOIUrl":"10.1007/s10068-025-01870-7","url":null,"abstract":"<p><p>This study demonstrated the commercialization potential of <i>Rana chensinensis</i> ovum protein isolates (RCOPI) by comparing it with commercially available proteins from physicochemical, structural, and functional properties to stabilizing Pickering emulsions. The size of RCOPI colloids nanoparticles reached 65 nm which is much smaller than the commercially available proteins. Besides, RCOPI exhibited incredible emulsion ability with the emulsion drop around 22 μm diameters and the zeta potential approximately - 50 mV. The emulsions stabilized by RCOPI showed remarkable stability and the Lactalysis index was about 21% after 100 days storage. In the encapsulation test, scanning electron microscopy (SEM) showed the size of the microcapsule ranging from 20 to 50 μm. The encapsulated efficiency of RCOPI stabilized emulsion was as good as the compared proteins, up to 75%. Furthermore, the results of the sensory evaluation showed that RCOPI stabilized emulsions received the highest approval ratings for appearance, taste, flavor and overall acceptability.</p><p><strong>Graphic abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10068-025-01870-7.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2455-2467"},"PeriodicalIF":2.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young-Jun Jung, Seung-Min Ahn, Hyungjae Lee, Myung-Ji Seo, Jae Kwang Kim, Dae-Ok Kim
{"title":"Quantification of carotenoids in Korean fruits and vegetables using reverse-phase HPLC","authors":"Young-Jun Jung, Seung-Min Ahn, Hyungjae Lee, Myung-Ji Seo, Jae Kwang Kim, Dae-Ok Kim","doi":"10.1007/s10068-025-01861-8","DOIUrl":"10.1007/s10068-025-01861-8","url":null,"abstract":"<div><p>Carotenoids are natural pigments that have health benefits, including antioxidant capacities. The ability to assess the carotenoid content of fruits and vegetables is an essential element of nutritional and food science applications. We analyzed the contents of 10 major carotenoids (capsorubin, capsanthin, lutein, zeaxanthin, β-cryptoxanthin, [13<i>Z</i>]-β-carotene, α-carotene, β-carotene, [9<i>Z</i>]-β-carotene, and lycopene) in 30 types of fruits and 39 types of vegetables grown in Korea along with their associated processed products using reverse-phase HPLC. Carotenoids were generally more abundant in vegetables than in fruits. The most common carotenoid was β-carotene. Fruits were rich in β-cryptoxanthin, while vegetables were rich in lutein and β-carotene. Both capsorubin and capsanthin were found exclusively in red paprika, while capsanthin was also present in red sweet pepper. Lycopene was found only in grapefruit. These findings provide a comprehensive database of carotenoid contents in fruits and vegetables grown in Korea and can serve as a valuable resource for food science and processing.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 10","pages":"2321 - 2330"},"PeriodicalIF":2.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimelanogenic effect of fermented licorice water extract on murine melanoma B16F10 cells.","authors":"Jin Yeong Jung, Ho Jeong Jeong, Gi Dong Han","doi":"10.1007/s10068-025-01878-z","DOIUrl":"10.1007/s10068-025-01878-z","url":null,"abstract":"<p><p>This study investigated the skin-whitening effect of fermented licorice water extract (FLWE) on B16F10 melanocytes. Bioconversion mediated by <i>Issatchenkia orientalis</i> MFST-based fermentation altered licorice's bioactive components. FLWE significantly increased polyphenol and flavonoid content compared with non-fermented licorice water extract (LWE). FLWE more effectively inhibited melanocyte-stimulating hormone-induced melanin production and tyrosinase activity than LWE. RT-PCR and Western blotting revealed that FLWE inhibits the expression of microphthalmia-associated transcription factor (MITF), the transcription factor for TRP-1, TRP-2, and tyrosinase. On analyzing FLWE's inhibitory activity against preexisting tyrosinase within melanocytes, FLWE did not directly inhibit tyrosinase itself, suggesting that FLWE exerts its whitening effect by inhibiting MITF activation. TLC and HPLC indicated that FLWE's superior whitening effect emanates from increased levels of aglycone compounds (isoliquiritigenin, glycyrrhizic acid, licochalcone A, and glabridin) after licorice fermentation. Overall, FLWE is a potentially effective skin-whitening material without side effects (vitiligo) commonly associated with direct tyrosinase inhibition.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10068-025-01878-z.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2571-2580"},"PeriodicalIF":2.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dendrobium officinale polysaccharides: alleviating UVB-induced skin damage by anti-oxidation and inhibiting matrix metalloproteinases expression","authors":"Zhejie Xu, Wanyi Zhou, Chenchen Yang, Ni Zhang, Jingrui Li, Wenyang Tao, Jianrong Xing, Ying Yang, Yali Dang","doi":"10.1007/s10068-025-01860-9","DOIUrl":"10.1007/s10068-025-01860-9","url":null,"abstract":"<div><p>Ultraviolet B (UVB) is a major external factor causing ultraviolet damage. This study assessed the protective effects of <i>Dendrobium officinale </i>polysaccharides (DOP) from both stems and leaves on UVB-induced skin damage in HaCaT Human Keratinocytes. Notably, stem DOPs (DOP1J, DOP3J, and DOP8J) outperformed leaf DOPs (DOP1Y, DOP3Y, and DOP8Y) in mitigating UVB-induced damage, with DOP1J and DOP8J demonstrating the highest efficacy in preserving cell proliferation. The DOPs exhibited antioxidant properties by enhancing superoxide dismutase, catalase, and glutathione peroxidase activities, and reducing reactive oxygen species levels. They curtailed cellular aging by inhibiting UVB-induced protein phosphorylation of p38, JNK, and ERK1/2 within the mitogen-activated protein kinase (MAPK) signaling pathway and by suppressing the protein expression of p53 and matrix metalloproteinases (MMPs, including MMP-1 and MMP-9). The mechanism underlying DOPs’ protective effects appears to be a dual-action approach: bolstering antioxidant defenses and dampening the MAPK pathway’s activation, thereby hindering the expression of MMPs.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 10","pages":"2307 - 2320"},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seung Hyun Yoo, Jeong Gyu Kim, Yong Jun Park, Jong-Oh Kim, Yong Bae Seo, Gun-Do Kim
{"title":"A quantitative polymerase chain reaction approach for distinguishing the geographical origin of <i>Pagrus major</i> from Korea and Japan.","authors":"Seung Hyun Yoo, Jeong Gyu Kim, Yong Jun Park, Jong-Oh Kim, Yong Bae Seo, Gun-Do Kim","doi":"10.1007/s10068-025-01877-0","DOIUrl":"10.1007/s10068-025-01877-0","url":null,"abstract":"<p><p>Illegal distribution and mislabeling of imported fishery products pose challenges to country-of-origin labeling regulations in Korea. To address this issue, a quantitative PCR (qPCR)-based method was developed to distinguish <i>Pagrus major</i> from Korea and Japan. Using genotyping-by-sequencing (GBS), two single nucleotide polymorphism (SNP) markers were identified, and allele-specific primers were designed. Gaussian mixture modeling established Ct thresholds, achieving accuracy of 81.67% and 77.78% for SNP001 and SNP008, respectively. The amplification efficiency and limit of detection (LOD) were assessed using tenfold serial dilutions (10-0.001 ng/μL). Standard curves for AA and TT homotypes showed high linearity (R<sup>2</sup> > 0.994) with amplification efficiencies of 103.65% and 97.63%, respectively. This qPCR-based method provides a reliable approach for origin verification of <i>P. major</i>, aiding regulatory enforcement and ensuring seafood authenticity.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10068-025-01877-0.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2657-2666"},"PeriodicalIF":2.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sung Ho Lee, Yerim Na, Jong Hyuk Lee, Chung Kil Park
{"title":"Sugar-free breadmaking using multi enzymes reactions.","authors":"Sung Ho Lee, Yerim Na, Jong Hyuk Lee, Chung Kil Park","doi":"10.1007/s10068-025-01880-5","DOIUrl":"10.1007/s10068-025-01880-5","url":null,"abstract":"<p><p>This study evaluated the potential and effectiveness of producing sugar-free white pan bread using transglucosidase and maltotetraose amylase. Proofing times, one of the main challenges for sugar-free white pan bread, of enzyme-treated groups were similar to those of the sucrose-added control. Specific volume measurements showed no significant differences between enzyme-treated and control groups. Color analysis revealed that enzyme-treated groups exhibited lower lightness (L*) and higher yellowness (b*) than negative control. Hardness increased over a 3-day storage period for all samples, but by the third day, enzyme-treated groups had lower hardness than control, indicating delayed staling process. Sugar content analysis indicated that enzyme-treated groups had the lowest maltose levels, with total sugar levels similar to the sugar-free control. In conclusion, use of transglucosidase and maltotetraose amylase effectively overcame major challenges such as underfermentation and poor texture in sugar-free bread, making it a simple and promising solution for sugar-free bread production.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2469-2474"},"PeriodicalIF":2.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of molecular markers for distinguishing between the edible plant <i>Petasites japonicus</i> and the toxic plant <i>Farfugium japonicum</i> using quantitative real-time polymerase chain reaction.","authors":"Cheol Min Kim, Cheol Seong Jang","doi":"10.1007/s10068-025-01874-3","DOIUrl":"10.1007/s10068-025-01874-3","url":null,"abstract":"<p><p>The consumption of wild plants, raw or cooked, is a common practice across many countries. However, some wild plants have toxins harmful to humans. In this study, we developed specific primer targeting chloroplast genes to distinguish the edible plant, <i>Petasites japonicus</i>, and the toxic plant, <i>Farfugium japonicum.</i> The performance of the designed primers was evaluated through a qPCR assay, assessing their specificity, sensitivity, and practical applicability. Six primer (three each for <i>P. japonicus</i> and <i>F. japonicum</i>) demonstrated linearity with correlation coefficients above 0.99 and slopes ranging from - 3.337 to - 3.581. PCR efficiencies were calculated to range from 90.28 to 97.86%, with a detection limit of 0.001 ng of DNA, and a quantitative threshold of 0.1% (w/w). The specificity was confirmed with 13 non-target plant species, and efficiency validated using 15 commercial samples. The assay help prevent mistaking toxic wild plants and provide guidelines to identify toxic plants for public safety.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10068-025-01874-3.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2553-2562"},"PeriodicalIF":2.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young Sung Jung, Chi Heung Cho, Mingyeong Kim, Tae Young Kwon, Yu Ri Choe, Nari Seo, Hyun-Soo Kim, Hyun Joo An, Sang-Hoon Lee
{"title":"Correlation between antioxidant capacity and advanced glycation endproducts inhibition in enzyme-treated polysaccharides from <i>Sargassum fusiforme</i>.","authors":"Young Sung Jung, Chi Heung Cho, Mingyeong Kim, Tae Young Kwon, Yu Ri Choe, Nari Seo, Hyun-Soo Kim, Hyun Joo An, Sang-Hoon Lee","doi":"10.1007/s10068-025-01867-2","DOIUrl":"10.1007/s10068-025-01867-2","url":null,"abstract":"<p><p>Marine polysaccharides demonstrate strong antioxidant capacity and can effectively alleviate oxidative stress. However, their high molecular weight often makes them resistant to enzymatic breakdown in the digestive system, limiting their bioavailability, and functional effectiveness. This study explored the potential of enzyme-treated polysaccharides derived from <i>Sargassum fusiforme</i> (EPS) for antioxidant and anti-glycation applications and elucidated their potential in managing oxidative stress. Polysaccharide from <i>S. fusiforme</i> was enzyme-treated using α-amylase, cellulase, and α-amylase derived from Caco-2 cells. EPSs generated were analyzed for molecular weight distribution and residual metal ion content such as arsenic. EPSs exhibit enhanced antioxidant capacities and advanced glycation end-product (AGEs) inhibition, highlighting their potential in addressing oxidative stress and AGEs-related diseases. Correlation and principal component analysis revealed that molecular weight reduction positively influenced the antioxidant capacity and AGE-breaking activities. These findings suggest that EPSs could serve as a natural therapeutic agents in functional foods and nutraceuticals, offering a promising approach for mitigating oxidative stress-induced health issues.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10068-025-01867-2.</p>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 11","pages":"2591-2600"},"PeriodicalIF":2.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enhanced encapsulation efficiency and stability of aronia anthocyanins by encapsulation: A focus on ionic strength of wall materials","authors":"Youngbin Jang, Eunmi Koh","doi":"10.1007/s10068-025-01875-2","DOIUrl":"10.1007/s10068-025-01875-2","url":null,"abstract":"<div><p>Polysaccharides with different zeta potential values, including guar gum (GG), gum Arabic (GA), and xanthan gum (XG), were combined with maltodextrin (MD) and then used as wall materials to encapsulate anthocyanins from aronia. Based on absolute zeta potential of 10 mV, wall materials were divided into two groups: MD (− 2.80 mV) and GG (− 3.49 mV) as non‐ionic materials and GA (− 30.83 mV) and XG (− 65.87 mV) as anionic materials. The addition of aronia concentrates (pH 3.8) had no effect on zeta potential values in non‐ionic MD and MD + GG, whereas it elevated zeta potential values in anionic MD + GA and MD + XG. Non‐ionic MD and MD + GG exhibited higher encapsulation efficiency and storage stability of anthocyanins than anionic MD + GA and MD + XG. Principal component analysis showed that zeta potential values of wall materials positively correlated with encapsulation efficiency and storage stability of anthocyanins. The results indicate that non‐ionic wall materials are suitable for anthocyanins encapsulation.</p></div>","PeriodicalId":566,"journal":{"name":"Food Science and Biotechnology","volume":"34 10","pages":"2197 - 2206"},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}