中国疫苗和免疫 Pub Date : 2009-10-01

Yin-Peng Da, Lin-Yun Luo, Li-Zhi Song

引用次数: 0

[Accuracy evaluation of diagnostic test in laboratory]. [实验室诊断试验的准确性评价]。

中国疫苗和免疫 Pub Date : 2009-10-01

Shou-Jun Zhao, Shuang Zhang, Feng Wang

引用次数: 0

[Evaluations of 5 kinds of China-made enzyme immunoassay kits for hepatitis B virus surface antigen detection]. [5种国产乙型肝炎病毒表面抗原酶免疫测定试剂盒的评价]。

中国疫苗和免疫 Pub Date : 2009-10-01

Yong Zhang, Feng Wang, Jing-Chen Ma

{"title":"[Evaluations of 5 kinds of China-made enzyme immunoassay kits for hepatitis B virus surface antigen detection].","authors":"Yong Zhang, Feng Wang, Jing-Chen Ma","doi":"","DOIUrl":"","url":null,"abstract":"Unlabelled: OBJECTIVE To Screen best EIA kit that fits best for using in serological detection of HBsAg in the national investigation of hepatitis B virus infection.Methods: Top 5 kinds of kits on the best seller list of China-made HBsAg EIA detection reagents were selected and each was detected the HBsAg panel (63 positive and 90 negative) 5 times. The s/n ratio of each test was recorded and transformed by log (s/n+1). Reliability indices ICC and CV, and validity indices AUC, pAUC and Se(FPR-e) were calculated and compared between each of the kits.Results: The indices of ICC and CV of the 5 kits arranged in the same order of E, C, D, B and A, from the best to the worst, and the differences between them were significantly important (Bootstrap method, P < 0.05); The AUCs ranged from 0.965 to 0.987, and for kits E and B which had the biggest pAUCs, differs significantly from kits A and D (Bootstrap method, P < 0.05). Kit E had the highest Se(FPR-e) which was significantly bigger than kit B (Bootstrap method, P < 0.05).Conclusion: The 5 kinds of kits are all excellent and kit E is the best.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28656466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Global pertussis epidemiology and immunization strategies]. [全球百日咳流行病学和免疫战略]。

中国疫苗和免疫 Pub Date : 2009-10-01

Wen-Hong Zhang

引用次数: 0

[Evaluation of 5 kinds of China-made enzyme immunoassay kits for antibody to hepatitis B virus e antigen detection]. [5种国产乙型肝炎病毒e抗原抗体酶免疫测定试剂盒的评价]。

中国疫苗和免疫 Pub Date : 2009-10-01

Feng Wang, Jing-Chen Ma, Tao Yu

{"title":"[Evaluation of 5 kinds of China-made enzyme immunoassay kits for antibody to hepatitis B virus e antigen detection].","authors":"Feng Wang, Jing-Chen Ma, Tao Yu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The aim of this study is to select a better one from five EIA kits made in China for the serological detection of anti-HBe in a national hepatitis B survey.Methods: Top 5 kinds of kits on the best seller list of China-made anti-HBe EIA detection were selected and each was detected the anti-HBe panel (66 positive and 88 negative) 5 times. The co/s ratio of each test was recorded and transformed by log(co/s+1). ICC and CV, the reliability indices and AUC, pAUC and Se(PR=e), the validity indices were calculated and compared between every two kits.Results: The indices of ICC and CV of the 5 kinds of kits arranged in the same order of A, E, C, D and B, from the best to the worst. For ICC, there were significantly different between every two of them (Bootstrap method, P < 0.05), except for between kit C and E. The magnitude ofAUCs were from 0.969 to 0.996; the orders of pAUC and Se(FPR=e) indices was C, E, D, A, B, and C, D, E, B, A respectively; both the indices of kit C were significantly higher than others (Bootstrap method, P < 0.05).Conclusion: All the 5 kinds of kits are excellent and kit C is the best.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28655892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of 5 kinds of China-made enzyme immunoassay kits for antibody to hepatitis B virus surface antigen detection]. [5种国产乙型肝炎病毒表面抗原抗体酶免疫测定试剂盒的评价]。

中国疫苗和免疫 Pub Date : 2009-10-01

Feng Wang, Jing-Chen Ma, Wen-Ying Zhang

{"title":"[Evaluation of 5 kinds of China-made enzyme immunoassay kits for antibody to hepatitis B virus surface antigen detection].","authors":"Feng Wang, Jing-Chen Ma, Wen-Ying Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To Screen an EIA kit that fits best for using in serological detection of anti-HBs in the national investigation of hepatitis B virus infection.Methods: Top 5 kinds of kits on the best seller list of China-made anti-HBs EIA detection kits were selected and each was detected the anti-HBs panel(44 positive and 108 negative) 5 times. The s/n ratio of each test was recorded and transformed by log(s/n+1). ICC and CV, the reliability indices and AUC, pAUC and Se(FPR=e), the validity indices were calculated and compared between each kit.Results: The indices of ICC and CV of the 5 kinds of kits arranged in the same order of D, E, B, C and A, from the best to the worst and the differences between them were significantly important (Bootstrap method, P < 0.05), except for between E and B in the index of CV; the AUCs ranged from 0.980 to 0.993 and pAUCs did not differ with each other, except with kit A, the lowest one. Kit E had the highest sensitivity which was higher than kits B, C and D when the specificity was fixed at 0.97, and the later kits were higher than kit A(Bootstrap method, P < 0.05).Conclusion: All the 5 kits are excellent and have synthetically considered the reliability and validity indicators and its function, kit D is the best.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28656467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Gene identification of mumps wild virus isolated firstly in Jilin province]. 吉林省首次分离的流行性腮腺炎野生病毒基因鉴定。

中国疫苗和免疫 Pub Date : 2009-10-01

Shuang Wang, Xin Chang, Jian-Hui Zhou

引用次数: 0

[Study on the influencing factors of hepatitis B vaccine timely birth dose for infants born at hospitals]. [医院出生婴儿乙肝疫苗及时出生剂量的影响因素研究]。

中国疫苗和免疫 Pub Date : 2009-10-01

Yuan-Sheng Hu, Zheng-Rong Ding, Guo-Fei Huang

{"title":"[Study on the influencing factors of hepatitis B vaccine timely birth dose for infants born at hospitals].","authors":"Yuan-Sheng Hu, Zheng-Rong Ding, Guo-Fei Huang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the coverage of timely birth dose of HepB vaccine (HepB1) and reasons contributed to the non-timely of HepB1 for the infants delivered in the hospital of remote and poverty areas in Yunnan province, and to bring forword the special strategies to improve the coverage of HepB of infants born at those hospitals.Methods: According to the reported coverage and the estimated coverage of HepB1 vaccine, the HepB coverage of infants born at hospitals was investigated by sampling in Zhaotong and Xishuangbanna in 2007.Results: The average coverage of HepB1 of infants born at the local hospitals was 67.19%, and the HepB1 coverage of infant was higher in county hospitals than in township hospitals. Infants with premature, low birth weight and asphyxia and the shortage of HepB vaccine were the mainly reasons to the non-timely HepB1 of infants born at hospitals.Conclusion: The HepB1 coverage of infants was still keep at low level in the local hospital; and to assure enough HepB vaccine in the village hospitals and to grasp immunization contraindication correctly were the two key strategies to promote the HepB1 coverage for infants born at the local hospitals.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28655894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Hemogglutinin gene and amino acid characteristic of measles virus in Jilin province, 2001-2008]. [2001-2008年吉林省麻疹病毒血凝素基因及氨基酸特征]。

中国疫苗和免疫 Pub Date : 2009-10-01

Jian-Hui Zhou, Zhan-Sen Du, Xin Chang

{"title":"[Hemogglutinin gene and amino acid characteristic of measles virus in Jilin province, 2001-2008].","authors":"Jian-Hui Zhou, Zhan-Sen Du, Xin Chang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the genetic characteristic and amino acid mutation of hemogglutinin (H) protein of measles viruses circulated in Jilin provine in 2001-2008.Methods: 5 representative strains of measles virus circulated in Jilin provine in 2001-2008 were chosen to conduct the RNA extraction and the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for amplifying H gene and to do sequence analysis of the PCR products, and then to compare with Chinese vaccine strain S191 and 23 kinds of genotypes of measles virus representative strains from genebank for analysis of phylogenetic, nucleotide and amino acid sequence identity and variation, glycosylated domains variation.Results: 5 strains of measles virus belong to H1 genotype, H1a sub-genotype. There were 6-17 (0.3%-0.9%) nucleotides and 3-9 (0.5%-1.5%) amino acids differences among 5 strains. There were 9-17 (0.5%-0.9%) nucleotides and 3-7 (0.5%-1.2%) amino acids differences between 5 strains and China 93-4, and 89-95 (4.8%-5.1%) nucleotides and 25-30 (4.1%-4.8%) amino acids differences between 5 strains and China vaccine S191. H protein of 5 measles virus all keep 4 glycosylation sites, the fifth glycosylation site NLS238-240 was losen because of the mutation from Set to Asn. in 240th amino acids.Conclusion: 5 measles virus circulated in Jilin province in 2001-2008 lose one glycosylation site which probably affected antigenencity. The nucleotides and amino acids differences among 5 strains were not too but both the nucleotides and amino acids identity showed falling trend year after year with both China 93-4 and S191. The H gene variation of measles virus in Jilin province was still increasing and accumulating year by year.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28655896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Safety and immunogenicity of lyophilized live attenuated domestic varicella vaccine]. [国产水痘冻干减毒活疫苗的安全性和免疫原性]。

中国疫苗和免疫 Pub Date : 2009-10-01

En-Fu Chen, Zheng-Gang Jiang, Qian Li

引用次数: 0

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