Tissue Engineering Part A最新文献_第6页

Primed IFN-γ-Umbilical Cord Stem Cells Ameliorate Temporomandibular Joint Osteoarthritis. IFN-γ-脐带干细胞可改善颞下颌关节骨关节炎。

IF 3.5 3区医学

Tissue Engineering Part A Pub Date : 2024-06-27 DOI: 10.1089/ten.TEA.2023.0370

Yerin Kim, Hyunjeong Kim, So-Yeon Yun, Bu-Kyu Lee

{"title":"Primed IFN-γ-Umbilical Cord Stem Cells Ameliorate Temporomandibular Joint Osteoarthritis.","authors":"Yerin Kim, Hyunjeong Kim, So-Yeon Yun, Bu-Kyu Lee","doi":"10.1089/ten.TEA.2023.0370","DOIUrl":"10.1089/ten.TEA.2023.0370","url":null,"abstract":"Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disorder affecting the temporomandibular joint (TMJ), marked by persistent inflammation and structural damage to the joint. Only symptomatic treatment is available for managing TMJOA. Human umbilical cord mesenchymal stem cells (hUC-MSCs) show potential for treating TMJOA via their immune-modulating actions in the disease area. In addition, stimulation of inflammatory cytokines such as interferon-gamma in hUC-MSCs improves the therapeutic activity of naïve stem cells. Emerging evidence indicates that macrophages play significant roles in regulating joint inflammation through diverse secreted mediators in the pathogenesis of TMJOA. This study was conducted to evaluate the effects of inflammatory cytokine-stimulated hUC-MSCs in repairing TMJOA-induced cartilage lesions and the role of macrophages in the disease. Our in vitro data showed that stimulated hUC-MSCs induce M2 polarization of macrophages and enhance the expression of anti-inflammatory molecules. These effects were subsequently validated in vivo. In a rat model of TMJOA, stimulated hUC-MSCs ameliorated inflammation and increased M2 macrophages ratio. Our results indicate that hUC-MSCs stimulated by inflammatory cytokines modulate the activation of M2 macrophages, thereby shifting the local osteoarthritis microenvironment toward a prochondrogenic state and facilitating cartilage repair in inflammatory conditions. Stimulating hUC-MSCs with inflammatory cytokines could potentially offer an effective therapeutic approach for TMJOA, with macrophages playing a pivotal role in immune modulation.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated Shear Stress Modulates Heterogenous Cellular Subpopulations to Induce Vascular Remodeling. 高剪切应力调节异源细胞亚群，诱导血管重塑。

IF 3.5 3区医学

Tissue Engineering Part A Pub Date : 2024-06-10 DOI: 10.1089/ten.TEA.2023.0362

Katharina S Fischer, Dominic Henn, Eric T Zhao, Dharshan Sivaraj, Ben Litmanovich, William W Hahn, Andrew C Hostler, Sultana M Mojadidi, Javier Gonzalez, Amelia B Knochel, Maria Gracia Mora Pinos, Jared Holley, Hudson Kussie, Maia Granoski, Jonathan P Yasmeh, Ulrich Kneser, Kellen Chen, Geoffrey C Gurtner

{"title":"Elevated Shear Stress Modulates Heterogenous Cellular Subpopulations to Induce Vascular Remodeling.","authors":"Katharina S Fischer, Dominic Henn, Eric T Zhao, Dharshan Sivaraj, Ben Litmanovich, William W Hahn, Andrew C Hostler, Sultana M Mojadidi, Javier Gonzalez, Amelia B Knochel, Maria Gracia Mora Pinos, Jared Holley, Hudson Kussie, Maia Granoski, Jonathan P Yasmeh, Ulrich Kneser, Kellen Chen, Geoffrey C Gurtner","doi":"10.1089/ten.TEA.2023.0362","DOIUrl":"10.1089/ten.TEA.2023.0362","url":null,"abstract":"Rationale: Elevated shear stress (ESS) induces vascular remodeling in veins exposed to arterial blood flow, which can lead to arteriovenous (AV) fistula failure. The molecular mechanisms driving remodeling have not been comprehensively examined with a single-cell resolution before. Objective: Using an in vivo animal mode, single-cell RNA sequencing, and histopathology, we precisely manipulate blood flow to comprehensively characterize all cell subpopulations important during vascular remodeling. Methods: AV loops were created in saphenous vessels of rats using a contralateral saphenous vein interposition graft to promote ESS. Saphenous veins with no elevated shear stress (NSS) were anastomosed as controls. Findings: ESS promoted transcriptional homogeneity, and NSS promoted considerable heterogeneity. Specifically, ESS endothelial cells (ECs) showed a more homogeneous transcriptional response promoting angiogenesis and upregulating endothelial-to-mesenchymal transition inhibiting genes (Klf2). NSS ECs upregulated antiproliferation genes such as Cav1, Cst3, and Btg1. In macrophages, ESS promoted a large homogeneous subpopulation, creating a mechanically activated, proinflammatory and thus proangiogenic myeloid phenotype, whereas NSS myeloid cells expressed the anti-inflammatory and antiangiogenetic marker Mrc1. Conclusion: ESS activates unified gene expression profiles to induce adaption of the vessel wall to hemodynamic alterations. Targeted depletion of the identified cellular subpopulations may lead to novel therapies to prevent excessive venous remodeling, intimal hyperplasia, and AV fistula failure.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Framing Bias Impacts Preferences for Innovation in Bone Tissue Engineering. 框架偏差如何影响骨组织工程的创新偏好。

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-10 DOI: 10.1089/ten.TEA.2023.0338

Markus Laubach, Stephen Whyte, Ho Fai Chan, Frank Hildebrand, Boris M Holzapfel, Ulrich Kneser, Uwe Dulleck, Dietmar W Hutmacher

{"title":"How Framing Bias Impacts Preferences for Innovation in Bone Tissue Engineering.","authors":"Markus Laubach, Stephen Whyte, Ho Fai Chan, Frank Hildebrand, Boris M Holzapfel, Ulrich Kneser, Uwe Dulleck, Dietmar W Hutmacher","doi":"10.1089/ten.TEA.2023.0338","DOIUrl":"10.1089/ten.TEA.2023.0338","url":null,"abstract":"It is currently unknown if surgeons and biomaterial scientists &or tissue engineers (BS&orTE) process and evaluate information in similar or different (un)biased ways. For the gold standard of surgery to move \"from bench to bedside,\" there must naturally be synergies between these key stakeholders' perspectives. Because only a small number of biomaterials and tissue engineering innovations have been translated into the clinic today, we hypothesized that this lack of translation is rooted in the psychology of surgeons and BS&orTE. Presently, both clinicians and researchers doubt the compatibility of surgery and research in their daily routines. This has led to the use of a metaphorical expression \"squaring of the circle,\" which implies an unsolvable challenge. As bone tissue engineering belongs to the top five research areas in tissue engineering, we choose the field of bone defect treatment options for our bias study. Our study uses an online survey instrument for data capture such as incorporating a behavioral economics cognitive framing experiment methodology. Our study sample consisted of surgeons (n = 208) and BS&orTE (n = 59). And we used a convenience sampling method, with participants (conference attendants) being approached both in person and through email between October 22, 2022, and March 13, 2023. We find no distinct positive-negative cognitive framing differences by occupation. That is, any framing bias present in this surgical decision-making setting does not appear to differ significantly between surgeon and BS&orTE specialization. When we explored within-group differences by frames, we see statistically significant (p < 0.05) results for surgeons in the positive frame ranking autologous bone graft transplantation lower than surgeons in the negative frame. Furthermore, surgeons in the positive frame rank Ilizarov bone transport method higher than surgeons in the negative frame (p < 0.05).","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspects of a Suspended Bioprinting System Affect Cell Viability and Support Bath Properties. 悬浮生物打印系统影响细胞存活率和支撑槽特性的各个方面

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2023-07-20 DOI: 10.1089/ten.TEA.2023.0097

Adam M Navara, Yilan Xu, Marissa R Perez, Antonios G Mikos

{"title":"Aspects of a Suspended Bioprinting System Affect Cell Viability and Support Bath Properties.","authors":"Adam M Navara, Yilan Xu, Marissa R Perez, Antonios G Mikos","doi":"10.1089/ten.TEA.2023.0097","DOIUrl":"10.1089/ten.TEA.2023.0097","url":null,"abstract":"Suspended hydrogel printing is a growing method for fabricating bioprinted hydrogel constructs, largely due to how it enables nonviscous hydrogel inks to be used in extrusion printing. In this work, a previously developed poly(N-isopropylacrylamide)-based thermogelling suspended bioprinting system was examined in the context of chondrocyte-laden printing. Material factors such as ink concentration and cell concentration were found to have a significant effect on printed chondrocyte viability. In addition, the heated poloxamer support bath was able to maintain chondrocyte viability for up to 6 h of residence within the bath. The relationship between the ink and support bath was also assessed by measuring the rheological properties of the bath before and after printing. Bath storage modulus and yield stress decreased during printing as nozzle size was reduced, indicating the likelihood that dilution occurs over time through osmotic exchange with the ink. Altogether this work demonstrates the promise for printing high-resolution cell-encapsulating tissue engineering constructs, while also elucidating complex relationships between the ink and bath, which must be taken into consideration when designing suspended printing systems.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9890068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal Three-Dimensional Printing for Micro-Modulation of Scaffold Stiffness Through Machine Learning. 通过机器学习对脚手架刚度进行微观调节的多模式3D打印。

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2023-10-26 DOI: 10.1089/ten.TEA.2023.0193

Wisarut Kiratitanaporn, Jiaao Guan, David B Berry, Alison Lao, Shaochen Chen

{"title":"Multimodal Three-Dimensional Printing for Micro-Modulation of Scaffold Stiffness Through Machine Learning.","authors":"Wisarut Kiratitanaporn, Jiaao Guan, David B Berry, Alison Lao, Shaochen Chen","doi":"10.1089/ten.TEA.2023.0193","DOIUrl":"10.1089/ten.TEA.2023.0193","url":null,"abstract":"The ability to precisely control a scaffold's microstructure and geometry with light-based three-dimensional (3D) printing has been widely demonstrated. However, the modulation of scaffold's mechanical properties through prescribed printing parameters is still underexplored. This study demonstrates a novel 3D-printing workflow to create a complex, elastomeric scaffold with precision-engineered stiffness control by utilizing machine learning. Various printing parameters, including the exposure time, light intensity, printing infill, laser pump current, and printing speed were modulated to print poly (glycerol sebacate) acrylate (PGSA) scaffolds with mechanical properties ranging from 49.3 ± 3.3 kPa to 2.8 ± 0.3 MPa. This enables flexibility in spatial stiffness modulation in addition to high-resolution scaffold fabrication. Then, a neural network-based machine learning model was developed and validated to optimize printing parameters to yield scaffolds with user-defined stiffness modulation for two different vat photopolymerization methods: a digital light processing (DLP)-based 3D printer was utilized to rapidly fabricate stiffness-modulated scaffolds with features on the hundreds of micron scale and a two-photon polymerization (2PP) 3D printer was utilized to print fine structures on the submicron scale. A novel 3D-printing workflow was designed to utilize both DLP-based and 2PP 3D printers to create multiscale scaffolds with precision-tuned stiffness control over both gross and fine geometric features. The described workflow can be used to fabricate scaffolds for a variety of tissue engineering applications, specifically for interfacial tissue engineering for which adjacent tissues possess heterogeneous mechanical properties (e.g., muscle-tendon).","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41167561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4D Printed Nerve Conduit with In Situ Neurogenic Guidance for Nerve Regeneration. 4D印刷神经导管与原位神经原性引导神经再生。

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2023-11-15 DOI: 10.1089/ten.TEA.2023.0194

Haitao Cui, Wei Zhu, Shida Miao, Kausik Sarkar, Lijie Grace Zhang

{"title":"4D Printed Nerve Conduit with In Situ Neurogenic Guidance for Nerve Regeneration.","authors":"Haitao Cui, Wei Zhu, Shida Miao, Kausik Sarkar, Lijie Grace Zhang","doi":"10.1089/ten.TEA.2023.0194","DOIUrl":"10.1089/ten.TEA.2023.0194","url":null,"abstract":"Nerve repair poses a significant challenge in the field of tissue regeneration. As a bioengineered therapeutic method, nerve conduits have been developed to address damaged nerve repair. However, despite their remarkable potential, it is still challenging to encompass complex physiologically microenvironmental cues (both biophysical and biochemical factors) to synergistically regulate stem cell differentiation within the implanted nerve conduits, especially in a facile manner. In this study, a neurogenic nerve conduit with self-actuated ability has been developed by in situ immobilization of neurogenic factors onto printed architectures with aligned microgrooves. One objective was to facilitate self-entubulation, ultimately enhancing nerve repairs. Our results demonstrated that the integration of topographical and in situ biological cues could accurately mimic native microenvironments, leading to a significant improvement in neural alignment and enhanced neural differentiation within the conduit. This innovative approach offers a revolutionary method for fabricating multifunctional nerve conduits, capable of modulating neural regeneration efficiently. It has the potential to accelerate the functional recovery of injured neural tissues, providing a promising avenue for advancing nerve repair therapies.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinted Human Lung Cancer-Mimics for Tissue Diagnostics Applications. 用于组织诊断应用的生物打印人类肺癌模拟物。

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2024-01-12 DOI: 10.1089/ten.TEA.2023.0149

Mian Wang, Wanlu Li, Regina Sanchez Flores, Ling Cai, Carlos Ezio Garciamendez-Mijares, Scott Gill, David Snyder, Jasmine Millabas, David Chafin, Yu Shrike Zhang, Azita Djalilvand

{"title":"Bioprinted Human Lung Cancer-Mimics for Tissue Diagnostics Applications.","authors":"Mian Wang, Wanlu Li, Regina Sanchez Flores, Ling Cai, Carlos Ezio Garciamendez-Mijares, Scott Gill, David Snyder, Jasmine Millabas, David Chafin, Yu Shrike Zhang, Azita Djalilvand","doi":"10.1089/ten.TEA.2023.0149","DOIUrl":"10.1089/ten.TEA.2023.0149","url":null,"abstract":"Developing a reproducible and secure supply of customizable control tissues that standardizes for the cell type, tissue architecture, and preanalytics of interest for usage in applications including diagnostic, prognostic, and predictive assays, is critical for improving our patient care and welfare. The conventionally adopted control tissues directly obtained from patients are not ideal because they oftentimes have different amounts of normal and neoplastic elements, differing cellularity, differing architecture, and unknown preanalytics, in addition to the limited supply availability and thus associated high costs. In this study, we demonstrated a strategy to stably produce tissue-mimics for diagnostics purposes by taking advantage of the three-dimensional (3D) bioprinting technology. Specifically, we take anaplastic lymphoma kinase-positive (Alk+) lung cancer as an example, where a micropore-forming bioink laden with tumor cells was combined with digital light processing-based bioprinting for developing native-like Alk+ lung cancer tissue-mimics with both structural and functional relevancy. It is anticipated that our proposed methodology will pave new avenues for both fields of tissue diagnostics and 3D bioprinting significantly expanding their capacities, scope, and sustainability.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71489477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial for Special Issue on "Bioprinting". 生物打印 "特刊编辑。

IF 4.1 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2024-04-26 DOI: 10.1089/ten.TEA.2024.0128

Lijie G Zhang, John Fisher

引用次数: 0

Three-Dimensional Bioprinting of Organoids: Past, Present, and Prospective. 有机体三维生物打印：过去、现在和未来。

IF 3.5 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2024-02-02 DOI: 10.1089/ten.TEA.2023.0209

Mariana Cabral, Ke Cheng, Donghui Zhu

引用次数: 0

Increasing Collagen to Bioink Drives Mesenchymal Stromal Cells-Chondrogenesis from Hyaline to Calcified Layers. 增加胶原到生物墨水驱动MSCs软骨形成从透明层到钙化层。

IF 3.5 3区医学

Tissue Engineering Part A Pub Date : 2024-06-01 Epub Date: 2023-11-30 DOI: 10.1089/ten.TEA.2023.0178

Océane Messaoudi, Christel Henrionnet, Edwin-Joffrey Courtial, Laurent Grossin, Didier Mainard, Laurent Galois, Damien Loeuille, Christophe Marquette, Pierre Gillet, Astrid Pinzano

{"title":"Increasing Collagen to Bioink Drives Mesenchymal Stromal Cells-Chondrogenesis from Hyaline to Calcified Layers.","authors":"Océane Messaoudi, Christel Henrionnet, Edwin-Joffrey Courtial, Laurent Grossin, Didier Mainard, Laurent Galois, Damien Loeuille, Christophe Marquette, Pierre Gillet, Astrid Pinzano","doi":"10.1089/ten.TEA.2023.0178","DOIUrl":"10.1089/ten.TEA.2023.0178","url":null,"abstract":"The bioextrusion of mesenchymal stromal cells (MSCs) directly seeded in a bioink enables the production of three-dimensional (3D) constructs, promoting their chondrogenic differentiation. Our study aimed to evaluate the effect of different type I collagen concentrations in the bioink on MSCs' chondrogenic differentiation. We printed 3D constructs using an alginate, gelatin, and fibrinogen-based bioink cellularized with MSCs, with four different quantities of type I collagen addition (0.0, 0.5, 1.0, and 5.0 mg per bioink syringe). We assessed the influence of the bioprinting process, the bioink composition, and the growth factor (TGF-ꞵ1) on the MSCs' survival rate. We confirmed the biocompatibility of the process and the bioinks' cytocompatibility. We evaluated the chondrogenic effects of TGF-ꞵ1 and collagen addition on the MSCs' chondrogenic properties through macroscopic observation, shrinking ratio, reverse transcription polymerase chain reaction, glycosaminoglycan synthesis, histology, and type II collagen immunohistochemistry. The bioink containing 0.5 mg of collagen produces the richest hyaline-like extracellular matrix, presenting itself as a promising tool to recreate the superficial layer of hyaline cartilage. The bioink containing 5.0 mg of collagen enhances the synthesis of a calcified matrix, making it a good candidate for mimicking the calcified cartilaginous layer. Type I collagen thus allows the dose-dependent design of specific hyaline cartilage layers.","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54232451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0