Tissue Engineering Part A最新文献

{"title":"Biosponges Embedded with GDNF Enhance Neuromuscular Recovery Following Volumetric Muscle Loss.","authors":"Jamshid Tadiwala, Connor Tobo, Kevin D Sekerak, Rebecca Sheetz, Amelia Ridolfo, Madhushika Elabada Gamage, Elif G Ertugral, Paul Jelliss, Matthew D Wood, Chandrasekhar R Kothapalli, Koyal Garg","doi":"10.1177/19373341261460266","DOIUrl":"10.1177/19373341261460266","url":null,"abstract":"<p><p>Volumetric muscle loss (VML) injuries result in an irrecoverable loss of muscle mass and function. VML injury causes loss of both contractile tissue and associated neuromuscular junctions (NMJs). Biosponge (BSG) scaffolds, composed of gelatin, collagen, and laminin-111, have improved recovery following VML. However, improvements in NMJ quantity were not observed. Glial cell line-derived neurotrophic factor (GDNF) is known to promote motor unit survival and stimulate neurite outgrowth. In this work, BSG scaffolds were electrostatically coupled with GDNF via gelatin nanoparticles (GNPs) to support myofiber regeneration and preserve NMJs post-VML in a rodent model. <i>In vitro</i> determination of release kinetics revealed an initial burst release of surface-bound GDNF with almost an equivalent amount of electrostatically bound GDNF retained within the BSG post 1 week of incubation at 37°C in phosphate-buffered saline. To create the VML injury in male Lewis rats (10-12 weeks old), ∼20% of the muscle mass was removed from the tibialis anterior (TA) muscle of both hindlimbs. Relative to BSG + GNP alone, treatment with BSG + GNP + GDNF showed a significant increase (∼25%) in peak isometric torque at 6 weeks postinjury. Qualitative and quantitative histological analysis of NMJs revealed an enhanced overlap between pre and postsynaptic structures in the BSG + GNP + GDNF group. Additionally, the incorporation of GDNF slowed BSG remodeling and degradation. Overall, these results suggest that BSG-mediated GDNF delivery is an effective strategy for mitigating NMJ loss and enhancing muscle recovery following VML.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"19373341261460266"},"PeriodicalIF":2.8,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.","authors":"Nathan J Castro, Greta Babakhanova, Ryan Donahue, Benjamin Bielajew, Jerry Hu, Kyriacos A Athanasiou","doi":"10.1177/19373341261447726","DOIUrl":"https://doi.org/10.1177/19373341261447726","url":null,"abstract":"<p><p>Because articular cartilage (AC) lacks inherent repair capacity, research has focused on translating tissue-engineered cartilage to the clinic. Toward this, rapid and nondestructive methods would be useful for determining in-process and release characteristics during the manufacture of tissue-engineered products. The current work aims to introduce a Raman-based methodology for nondestructive qualitative and quantitative characterization of tissue development using AC. First, Raman shifts associated with critical biochemical components of AC, with particular emphasis on DNA, glycosaminoglycans (long chains of sugar molecules and a key component of cartilage), total collagen, as well as pyridinoline (a marker of collagen crosslinking and maturation), were collated. Next, verification of the molecular spectroscopic biomarkers was conducted by temporally tracking tissue maturation/development of nascent and mature AC, establishing a temporal reference dataset. Finally, validation was performed by correlating the spectroscopic biomarkers with traditional photometric biochemical assays and mass spectrometry. The results presented here include a Maturity Index for quantification of tissue development/maturation. Strong correlations were found between nondestructive spectroscopic-based measurements and destructive (photometric and mass spectrometric) measurements with high linearity for both nondestructive Raman (<i>R</i>² > 0.96) and destructive biochemical (<i>R</i>² > 0.97) assays, respectively. Uses of the proposed rapid and nondestructive method include in-line quality assessment (in which the sample is not removed from the process stream) to monitor the manufacturing of tissue-engineered medical products. This study shows that Raman spectroscopy has the capacity of being a powerful tool for nondestructive quality control and assurance in traditional biomanufacturing workflows, and the approach taken here may also be utilized as a template and research tool for studies on the development of other native and engineered tissues.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"19373341261447726"},"PeriodicalIF":2.8,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation Modulates the Mechanoresponse of Bone-Homing Triple-Negative Breast Cancer Cells.","authors":"Shreya Venkatesh, Andrea Ordonez, William R Thompson, Edward B Chuong, Maureen E Lynch","doi":"10.1177/19373341261449883","DOIUrl":"https://doi.org/10.1177/19373341261449883","url":null,"abstract":"<p><p>Bone is a common site of breast cancer metastasis, which dramatically increases fracture risk. Recent <i>in vivo</i> bone metastasis studies show that mechanical loading is osteoprotective; however, little is known about how loading regulates breast cancer cell function in the unique bone mechanical environment, especially in combination with radiotherapy, one of the first-line treatments for advanced breast cancer patients. Here, we characterize the breast cancer cell response to a range of bone-mimicking fluid shears and determine how irradiation further modulates one candidate gene: <i>SERPINE1</i>. We found that irradiation, regardless of dosage, modulates <i>SERPINE1</i> expression and is sensitive to the timing of administration. Additionally, protein expression of <i>SERPINE1</i> accompanies a protumorigenic gene expression profile, which is elevated with higher-magnitude fluid shear stresses in a bone-mimicking 3D environment. Thus, we postulate that plasminogen activator inhibitor-1 (PAI-1) (encoded by <i>SERPINE1</i>) is a critical growth factor contributing to osteolytic lesion development in the bone metastatic vicious cycle of breast cancer.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"19373341261449883"},"PeriodicalIF":2.9,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148221182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Bioprinted Dual-Layered Corneal Structure Using Collagen-Based Bioinks.","authors":"Huasheng Huang, Yunong Yuan, Yuan Fang, Chris Hodge, Constantinos Pestglou, Gordon G Wallace, Gerard Sutton, Jingjing You","doi":"10.1177/19373341261424272","DOIUrl":"10.1177/19373341261424272","url":null,"abstract":"<p><p>Bioengineered corneal constructs are a promising solution to the global shortage of donor tissue. However, most current models lack anatomical curvature and appropriate extracellular matrix (ECM) composition of the native cornea, limiting their relevance for studying graft integration and stromal-endothelial interactions. In this study, we developed a bioprinted, dual-layer corneal model comprising corneal stromal cells laden in type I collagen (Col-I) and a monolayer of corneal endothelial cells supported by collagen type IV (Col-IV). The construct was printed onto a curved support to replicate the posterior curvature of the native cornea. The use of ECM-specific, human-derived collagen bioinks supported high cell viability (>90%) and the formation of a continuous endothelial layer. Histological and immunofluorescence analyses confirmed distinct layering and appropriate cellular morphology and phenotypic marker expression for both corneal stromal and endothelial cells. The construct retained its curvature, transparency, and interfacial integrity over 3 weeks in culture and demonstrated adherence when positioned over an <i>ex vivo</i> corneal tissue. This anatomically curved, multilayered <i>in vitro</i> model offers a physiologically relevant platform for exploring stromal-endothelial architecture and cell interaction in corneal tissue engineering applications.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"423-436"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147313104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Surface Modification of 3D Printed Scaffolds for Neurovascularized Nipple-Areolar Complexes.","authors":"Caitlin R O'Donnell, M Azam Ali, Jaydee D Cabral","doi":"10.1177/19373341251404450","DOIUrl":"10.1177/19373341251404450","url":null,"abstract":"<p><p>For individuals undergoing mastectomy, reconstruction of the nipple-areola complex (NAC) is a critical step in emotional and psychological recovery. However, current clinical approaches-including flap suturing, tattooing, or grafting-are limited by loss of projection, poor mechanical stability, and absence of sensation. Additive manufacturing and tissue engineering offer promising alternatives by enabling the development of hybrid scaffolds that maintain long-term projection and support the potential return of sensation. This review summarizes the state-of-the-art in NAC reconstruction and highlights how advances in additive manufacturing can address existing limitations. Emerging scaffold design strategies allow precise fabrication of biomimetic architectures that replicate the anatomical form and function of the NAC, while supporting tissue integration and mechanical durability. The use of biocompatible polymers such as poly-ε-caprolactone, combined with bioactive coatings and plasma surface modification, enhances cell attachment and vascularization. Additionally, the incorporation of stem cells, multicellular constructs, and conducting polymers is explored to enable multifunctional tissue regeneration and restore sensation through electrical stimulation. By integrating innovations in biomaterials science, regenerative medicine, and advanced fabrication technologies, the field is moving toward nipple reconstructions that are not only more life-like in appearance but also biologically responsive and sensate.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"379-392"},"PeriodicalIF":2.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145859230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocellulose Source and Morphology Impact on Properties of Composite Gelatin Hydrogel Scaffolds.","authors":"Janet V Reid, Niki Hazelton, Stefan J Hill, Marie-Joo Le Guen, Lyn M Wise","doi":"10.1177/19373341251410203","DOIUrl":"10.1177/19373341251410203","url":null,"abstract":"<p><p>Nanocellulose has emerged as a promising biomaterial for development of scaffolds for tissue engineering. Incorporation of nanocellulose into a polymer scaffold can increase its stiffness, allowing it to better mimic the mechanical properties of native extracellular matrix. Plant-derived nanocellulose is classified as either cellulose nanofibrils (CNFs) or cellulose nanocrystals (CNCs) depending on particle characteristics and extraction methods. Although both materials have been used in hydrogel composites, the impact of nanocellulose source and morphology on scaffold properties remains unclear. Here, we isolated high aspect ratio CNFs from two macroalgae species and compared them with conventional wood pulp-derived CNFs and CNCs in the preparation of composite gelatin hydrogels. All nanocellulose types increased hydrogel stiffness in a concentration-dependent manner; however, the greatest increase was achieved using brown algae CNF, where the addition of 1.25 wt.% nanocellulose resulted in a 5.2-fold increase in compression modulus relative to neat gelatin. Bioassays showed that nanocellulose improved keratinocyte adhesion and spreading on gelatin scaffolds, with a positive correlation between nanocellulose concentration and surface coverage and inverse with cell circularity. These findings demonstrate the influence of nanocellulose source and morphology on the mechanical and biological properties of composite scaffolds and highlight the potential of novel nanocellulose sources for scaffold development.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"409-422"},"PeriodicalIF":2.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145866512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keratin 3D Printing: Sustainable Biomaterials for Regenerative Medicine and Biomanufacturing.","authors":"Mina Rajabi, Xiaoxuan Deng, Jithendra Ratnayake, Maree Gould","doi":"10.1177/19373341251398721","DOIUrl":"10.1177/19373341251398721","url":null,"abstract":"<p><p>Keratin as an abundantly available natural protein from sources such as hair, wool, and feathers possesses excellent biocompatibility, biodegradability, and bioactivity that support cell growth. Recent advances in extracting, purifying, and characterizing keratin have led to the development of various keratin-based biomaterials, such as fibers, gels, films, and nanoparticles via conventional fabrication methods. However, these biomaterials are often limited by simple geometries, weak mechanical strength, and limited reproducibility. Emerging 3D printing technologies offer a promising alternative, allowing the creation of keratin-based scaffolds with precise architecture, tunable mechanical strength, and reproducible geometries. Despite keratin's abundance and biological advantages, the use of keratin in 3D printing remains relatively underexplored. This review provides a comprehensive overview of keratin's molecular structure and biochemistry, its diverse natural sources, extraction and purification methodologies, and the cross-linking mechanisms (chemical, UV, and enzymatic) used to formulate printable keratin-based inks. Furthermore, it discusses the biomedical applications of keratin-derived bioinks in tissue engineering and additive biomanufacturing, with emphasis on skin and bone regeneration. Combining keratin's biological functionality with the design flexibility of 3D printing offers a sustainable and cost-effective pathway toward next-generation biomaterials for regenerative medicine.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"365-378"},"PeriodicalIF":2.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Pot Crosslinking Enables 3D Printing of Shear-Recovery Fish Collagen VEGF-Functionalized Inks.","authors":"Alexandria R Leonard, Mathew H Cumming, M Azam Ali, Jaydee D Cabral","doi":"10.1177/19373341251405177","DOIUrl":"10.1177/19373341251405177","url":null,"abstract":"<p><p>Fish collagen is gaining attention as a sustainable biomaterial for three-dimensional (3D) scaffold fabrication in tissue engineering. In this study, a biomimetic, one-pot crosslinking strategy for native fish skin collagen was developed and compared with a conventional periodate oxidation-Schiff base approach using oxidized maltose. Both approaches increased viscosity and thermal stability while preserving native structural features. The oxidized maltose-crosslinked collagen demonstrated Schiff-base crosslinking, and the one-pot crosslinking method produced a covalent bond that was not a Schiff base. Precrosslinked collagens were processed into microgels and incorporated into calcium alginate to yield a shear-recovering, extrudable ink suitable for 3D extrusion printing. The printed scaffolds maintained structural resilience under physiological conditions, exhibited shear-recovery behavior confirmed by rheological analysis, and supported high cell viability. To enhance biofunctionality, vascular endothelial growth factor was conjugated to the 3D scaffolds, which were subsequently seeded with human bone marrow-derived mesenchymal stem cells. Immunofluorescence staining indicated endothelial lineage differentiation, suggesting that this platform may support the development of vascularized 3D tissue constructs.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"393-408"},"PeriodicalIF":2.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 and Tissue Engineering: What Have We Accomplished in the Past 5 Years?","authors":"Walid K Bibi, Avha R Mohanty, Alexander M Tatara","doi":"10.1177/19373341261449891","DOIUrl":"https://doi.org/10.1177/19373341261449891","url":null,"abstract":"<p><p>The Coronavirus Disease of 2019 (COVID-19) pandemic caused by SARS-CoV-2 resulted in widespread mortality, economic strain, and healthcare system disruption, highlighting the need for effective strategies to address viral threats. Tissue engineering and biomaterials can contribute significantly in advancing our understanding and treatment of respiratory viral infections by developing physiologically relevant <i>in vitro</i> models, controlled and targeted drug delivery systems, and effective next-generation vaccine platforms. Over the last 5 years, tissue-engineered cell-based models, including organ-on-a-chip systems, have improved our understanding of viral entry, immune response, and therapeutic efficacy in pulmonary, cardiac, neurological, and vascular tissue. Biomaterial-based delivery systems have enhanced the targeting, bioavailability, and sustained delivery of therapeutics such as antivirals, monoclonal antibodies, nucleic-acid-based vaccines, and anti-inflammatory drugs, while also reducing or eliminating system toxicity and our reliance on intravenous administration. Advancements in vaccine platforms using lipid nanoparticles, protein scaffolds, and vesicular or cell-based platforms are in different phases of development to stimulate more robust responses against emerging SARS-CoV-2 variants. Collectively, these advancements highlight the influence of tissue engineering and biomaterials in SARS-CoV-2 research and treatment. This review provides an overview of recent developments in <i>in vitro</i> modeling, drug delivery systems, and vaccine platforms, highlighting their future role in improving clinical outcomes, managing variants, and preparing for potential future pandemics, including addressing challenges in infrastructure such as limited access to high-containment biosafety facilities used to study emerging infectious pathogens.Impact StatementDuring the COVID-19 pandemic, we introduced basic tenets of virology to a tissue engineering audience and proposed different areas in which the field could contribute to developing diagnostics and therapeutics for respiratory viral infections. In this 5-year update, we highlight how tissue engineers and biomaterial scientists contributed tools to dissect virus pathophysiology and deliver therapies such as mRNA vaccines to prevent mortality during the pandemic. With the emergence of new variants and the threat of new respiratory viral pandemics, tissue engineers can continue to play important roles in virology.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"19373341261449891"},"PeriodicalIF":2.9,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148007509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jujuboside A-Loaded Exosomes Alleviate Pulmonary Fibrosis via TGF-β/Smad and Autophagy Regulation.","authors":"Jing Liu, Shuli Zhang, Yi Sun, Minghao Gu, Xuedong Liu","doi":"10.1177/19373341261449902","DOIUrl":"https://doi.org/10.1177/19373341261449902","url":null,"abstract":"<p><p>This study aimed to scrutinize the antifibrotic effects of jujuboside A-loaded exosomes (JuA-Exo) on TGF-β1-induced MRC-5 fibroblasts and explore the pathophysiological basis. Exosomes were isolated from human mesenchymal stem cells and loaded with JuA by ultrasonic dispersion. A TGF-β1-induced MRC-5 fibrosis model was established. Cell viability, migration capacity, levels of inflammatory cytokines, extracellular matrix components, and autophagy-related markers were assessed. To determine autophagy dependence, the autophagy inhibitor 3-methyladenine (3-MA) was employed. JuA-Exo significantly attenuated fibroblast activation triggered by TGF-β1, decreased the secretion of IL-6, IL-1β, and hydroxyproline, and downregulated fibrotic gene expression, including α-SMA, fibronectin, COL1A1, and COL3A1. Mechanistically, JuA-Exo was associated with modulation of the TGF-β/Smad signaling pathway and restoration of autophagy-related markers. Importantly, the defensive effects of JuA-Exo were reversed upon autophagy inhibition by 3-MA. JuA-Exo exerts antifibrotic and anti-inflammatory effects, which are associated with modulation of TGF-β/Smad signaling and autophagy-related processes; however, the mechanistic linkage between these pathways and their causal roles requires further validation. These findings highlight JuA-Exo as a potential experimental candidate, although the current evidence is limited to <i>in vitro</i> observations and does not yet support therapeutic application.Impact StatementThis study demonstrates that jujuboside A-loaded exosomes exert antifibrotic and anti-inflammatory effects <i>in vitro</i>, providing mechanistic insight and highlighting a potential nanotherapeutic approach that warrants further validation.</p>","PeriodicalId":56375,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"19373341261449902"},"PeriodicalIF":2.9,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148007641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}