Nanobiomedicine最新文献_第4页

Bioavailability of Orally Delivered Alpha-Tocopherol by Poly(Lactic-Co-Glycolic) Acid (PLGA) Nanoparticles and Chitosan Covered PLGA Nanoparticles in F344 Rats. 聚乳酸-共甘醇酸（PLGA）纳米颗粒和壳聚糖包裹的 PLGA 纳米颗粒口服α-生育酚在 F344 大鼠体内的生物利用率

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/63305

Lacey C Simon, Rhett W Stout, Cristina Sabliov

{"title":"Bioavailability of Orally Delivered Alpha-Tocopherol by Poly(Lactic-Co-Glycolic) Acid (PLGA) Nanoparticles and Chitosan Covered PLGA Nanoparticles in F344 Rats.","authors":"Lacey C Simon, Rhett W Stout, Cristina Sabliov","doi":"10.5772/63305","DOIUrl":"10.5772/63305","url":null,"abstract":"It is hypothesized that the bioavailability of αT (alpha-tocopherol), an antioxidant, can be improved when delivered by poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) and chitosan covered PLGA nanoparticles (PLGA-Chi NPs), and that the mucoadhesive properties of chitosan may enhance absorption of αT. PLGA and PLGA-Chi NPs were characterized by measuring entrapment efficiency, size, polydispersity, and zeta potential. Nanoparticle physical stability, chemical stability of entrapped αT, and release kinetics were also measured. Pharmacokinetic studies were conducted by administering PLGA (αT) NPs, PLGA-Chi (αT) NPs, and free αT via oral gavage in rats. The size and zeta potential of the two particle systems were 97.87 ± 2.63 nm and -36.2 ± 1.31 mV for PLGA(αT) NPs, and 134 ± 2.05 nm and 38.0 ± 2.90 mV for PLGA-Chi (αT) nanoparticles in DI water. The particle systems showed to be stable during various in vitro assays. Bioavailability of nanodelivered αT was improved compared to the free αT, by 170% and 121% for PLGA and PLGA-Chi NPs, respectively. It was concluded that while chitosan did not further improved bioavailability of αT, PLGA NPs protected the entrapped drug from the GI environment degradation and proved to be an effective delivery system for αT.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formyl-Peptide Receptor Agonists and Amorphous SiO₂-NPs Synergistically and Selectively Increase the Inflammatory Responses of Human Monocytes and PMNs. 甲酰基肽受体激动剂和无定形SiO2-NPs协同和选择性地增加人单核细胞和PMNs的炎症反应。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62251

Regina Tavano, Daniela Segat, Chiara Fedeli, Giulia Malachin, Elisa Lubian, Fabrizio Mancin, Emanuele Papini

{"title":"Formyl-Peptide Receptor Agonists and Amorphous SiO2-NPs Synergistically and Selectively Increase the Inflammatory Responses of Human Monocytes and PMNs.","authors":"Regina Tavano, Daniela Segat, Chiara Fedeli, Giulia Malachin, Elisa Lubian, Fabrizio Mancin, Emanuele Papini","doi":"10.5772/62251","DOIUrl":"https://doi.org/10.5772/62251","url":null,"abstract":"We tested whether amorphous SiO2-NPs and formylpeptide receptor (FPRs) agonists synergistically activate human monocytes and neutrophil polymorphonuclear granulocytes (PMNs). Peptide ligands specifically binding to FPR1 (f-MLP) and to FPR2 (MMK-1, WKYMVM and WKYMVm) human isoforms did not modify the association of SiO2-NPs to both cell types or their cytotoxic effects. Similarly, the extent of CD80, CD86, CD83, ICAM-1 and MHCII expression in monocytes treated with SiO2-NPs was not significantly altered by any FPRs agonist. However, FPR1 stimulation with f-MLP strongly increased the secretion of IL-1β, IL-6 and IL-8 by human monocytes, and of IL-8 by PMNs in the presence of SiO2-NPs, due to the synergic stimulation of gene transcription. FPR2 agonists also up-modulated the production of IL-1β induced by monocytes treated with SiO2-NPs. In turn, SiO2-NPs increased the chemotaxis of PMNs toward FPR1-specific ligands, but not toward FPR2-specific ones. Conversely, the chemotaxis of monocytes toward FPR2-specific peptides was inhibited by SiO2-NPs. NADPH-oxidase activation triggered by FPR1- and FPR2-specific ligands in both cell types was not altered by SiO2-NPs. Microbial and tissue danger signals sensed by FPRs selectively amplified the functional responses of monocytes and PMNS to SiO2-NPs, and should be carefully considered in the assessment of the risk associated with nanoparticle exposure.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36257595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

saltPAD: A New Analytical Tool for Monitoring Salt Iodization in Low Resource Settings. saltPAD:低资源环境下监测盐碘化的新分析工具。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62919

Nicholas M Myers, Emmerentia Elza Strydom, James Sweet, Christopher Sweet, Rebecca Spohrer, Muhammad Ali Dhansay, Marya Lieberman

引用次数: 3

Designer DNA Architectures: Applications in Nanomedicine. 设计DNA架构:在纳米医学中的应用。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/63228

Arun Richard Chandrasekaran

引用次数: 7

Turning the Page to Year 2016. 翻开2016年。

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/62174

Markus Dettenhofer, Winston Patrick Kuo

引用次数: 0

In Vitro AuNPs' Cytotoxicity and Their Effect on Wound Healing. 体外AuNPs的细胞毒性及其对伤口愈合的影响。

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/61132

Veronika Pivodová, Jana Franková, Adéla Galandáková, Jitka Ulrichová

{"title":"In Vitro AuNPs' Cytotoxicity and Their Effect on Wound Healing.","authors":"Veronika Pivodová, Jana Franková, Adéla Galandáková, Jitka Ulrichová","doi":"10.5772/61132","DOIUrl":"https://doi.org/10.5772/61132","url":null,"abstract":"Recently, due to their unique properties, gold nanoparticles (AuNPs) have been used in many biological applications. However, little is known about their toxicity when they come into contact with a biological system. Based on the proposal that AuNPs can have a positive effect on wound healing, the present study investigated the influence of negatively-charged-surface AuNPs (average diameter 25-50 nm) on the viability of normal human dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK). Moreover, we evaluated the effect of AuNPs on the secretion of proteins involved in wound healing, such as interleukin-8 and - 12 (IL-8, IL-12), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), basic fibroblast grow factor (bFGF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). The results showed that AuNPs were not toxic to NHDF and NHEK. They showed a decrease in AuNPs' production of pro-inflammatory cytokines IL-6, IL-12 and TNF-α, as well as proteins involved in angiogenesis such as VEGF and bFGF. Thus, we suggest that AuNPs could have anti-inflammatory and anti-angiogenic activity.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"2 ","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/61132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36257133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Nice Finish to 2014 and Looking Forward to 2015. 2014年圆满结束，期待2015年。

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/60139

Markus Dettenhofer, Winston Patrick Kuo

引用次数: 13

Single-Molecule Detection in Nanogap-Embedded Plasmonic Gratings. 纳米隙内嵌等离子体光栅中的单分子检测。

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/61094

Biyan Chen, Avinash Pathak, Keshab Gangopadhyay, Peter V Cornish, Shubhra Gangopadhyay

{"title":"Single-Molecule Detection in Nanogap-Embedded Plasmonic Gratings.","authors":"Biyan Chen, Avinash Pathak, Keshab Gangopadhyay, Peter V Cornish, Shubhra Gangopadhyay","doi":"10.5772/61094","DOIUrl":"https://doi.org/10.5772/61094","url":null,"abstract":"We introduce nanogap-embedded silver plasmonic gratings for single-molecule (SM) visualization using an epifluorescence microscope. This silver plasmonic platform was fabricated by a cost-effective nano-imprint lithography technique, using an HD DVD template. DNA/ RNA duplex molecules tagged with Cy3/Cy5 fluorophores were immobilized on SiO2-capped silver gratings. Light was coupled to the gratings at particular wavelengths and incident angles to form surface plasmons. The SM fluorescence intensity of the fluorophores at the nanogaps showed approximately a 100-fold mean enhancement with respect to the fluorophores observed on quartz slides using an epifluorescence microscope. This high level of enhancement was due to the concentration of surface plasmons at the nanogaps. When nanogaps imaged with epifluorescence mode were compared to quartz imaged using total internal reflection fluorescence (TIRF) microscopy, more than a 30-fold mean enhancement was obtained. Due to the SM fluorescence enhancement of plasmonic gratings and the correspondingly high emission intensity, the required laser power can be reduced, resulting in a prolonged detection time prior to photobleaching. This simple platform was able to perform SM studies with a low-cost epifluorescence apparatus, instead of the more expensive TIRF or confocal microscopes, which would enable SM analysis to take place in most scientific laboratories.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"2 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/61094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36257134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Lab-on-a-Tip (LOT): Where Nanotechnology Can Revolutionize Fibre Optics. 尖端实验室(LOT):纳米技术可以彻底改变光纤。

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/60518

Qimin Quan, Yiying Zhang

引用次数: 10

Addressing Barriers to the Development and Adoption of Rapid Diagnostic Tests in Global Health 在全球卫生领域解决发展和采用快速诊断测试的障碍

Nanobiomedicine Pub Date : 2015-01-01 DOI: 10.5772/61114

E. Miller, H. Sikes

{"title":"Addressing Barriers to the Development and Adoption of Rapid Diagnostic Tests in Global Health","authors":"E. Miller, H. Sikes","doi":"10.5772/61114","DOIUrl":"https://doi.org/10.5772/61114","url":null,"abstract":"Immunochromatographic rapid diagnostic tests (RDTs) have demonstrated significant potential for use as point-of-care diagnostic tests in resource-limited settings. Most notably, RDTs for malaria have reached an unparalleled level of technological maturity and market penetration, and are now considered an important complement to standard microscopic methods of malaria diagnosis. However, the technical development of RDTs for other infectious diseases, and their uptake within the global health community as a core diagnostic modality, has been hindered by a number of extant challenges. These range from technical and biological issues, such as the need for better affinity agents and biomarkers of disease, to social, infrastructural, regulatory and economic barriers, which have all served to slow their adoption and diminish their impact. In order for the immunochromatographic RDT format to be successfully adapted to other disease targets, to see widespread distribution, and to improve clinical outcomes for patients on a global scale, these challenges must be identified and addressed, and the global health community must be engaged in championing the broader use of RDTs.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/61114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70985664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43