Nanobiomedicine最新文献_第3页

Nanobiosensors in diagnostics. 纳米生物传感器在诊断中的应用。

Nanobiomedicine Pub Date : 2016-11-24 eCollection Date: 2016-01-01 DOI: 10.1177/1849543516663574

Alejandro Chamorro-Garcia, Arben Merkoçi

引用次数: 56

Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.). 新型脂质给药系统的开发、体外和体内评价(P. Beauv.)。

Nanobiomedicine Pub Date : 2016-11-22 eCollection Date: 2016-01-01 DOI: 10.1177/1849543516673445

Chukwuebuka Umeyor, Emmanuel Anaka, Franklin Kenechukwu, Chinazom Agbo, Anthony Attama

{"title":"Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.).","authors":"Chukwuebuka Umeyor, Emmanuel Anaka, Franklin Kenechukwu, Chinazom Agbo, Anthony Attama","doi":"10.1177/1849543516673445","DOIUrl":"https://doi.org/10.1177/1849543516673445","url":null,"abstract":"Newbouldia laevis (P. Beauv.) is a tropical rainforest plant used in traditional folk medicine for the treatment of malaria, cough, joint pains, stomach ache, oedema and inflammation. The main thrust of this research work was to study the analgesic/anti-nociceptive properties of N. laevis-loaded solid lipid microdispersions. N. laevis leaves were extracted using ethanol, and the extract was formulated into solid lipid microdispersions using lipid matrix comprising a rational blend of Precirol® ATO 5 and Softisan® 154. Characterization of the solid lipid microdispersions include determination of morphology, particle size, pH, thermal property, encapsulation efficiency percentage and analgesic/anti-nociceptive property. The results obtained showed that the particles were spherical with sizes ranging from 40 µm to 125 µm. The solid lipid microdispersions maintained a stable pH within the acidic region of 5-6 with insignificant variations (p > 0.05) over a period of 90 days. Thermal analysis showed that N. laevis was entrapped in the lipid matrix used for the formulations. Solid lipid microdispersions recorded a maximum encapsulation efficiency up to 88.1%. N. laevis-loaded solid lipid microdispersions also produced good analgesic/anti-nociceptive property comparable with the standard diclofenac potassium. N. laevis-loaded solid lipid microdispersions showed good analgesic/anti-nociceptive effect and could be used in the treatment and management of pain.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"1849543516673445"},"PeriodicalIF":0.0,"publicationDate":"2016-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849543516673445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Modulation of the contractility of micropatterned myocardial cells with nanoscale forces using atomic force microscopy. 使用原子力显微镜的纳米级力对微图案心肌细胞收缩性的调节。

Nanobiomedicine Pub Date : 2016-11-16 eCollection Date: 2016-01-01 DOI: 10.1177/1849543516675348

Neerajha Nagarajan, Varun Vyas, Bryan D Huey, Pinar Zorlutuna

{"title":"Modulation of the contractility of micropatterned myocardial cells with nanoscale forces using atomic force microscopy.","authors":"Neerajha Nagarajan, Varun Vyas, Bryan D Huey, Pinar Zorlutuna","doi":"10.1177/1849543516675348","DOIUrl":"https://doi.org/10.1177/1849543516675348","url":null,"abstract":"The ability to modulate cardiomyocyte contractility is important for bioengineering applications ranging from heart disease treatments to biorobotics. In this study, we examined the changes in contraction frequency of neonatal rat cardiomyocytes upon single-cell-level nanoscale mechanical stimulation using atomic force microscopy. To measure the response of same density of cells, they were micropatterned into micropatches of fixed geometry. To examine the effect of the substrate stiffness on the behavior of cells, they were cultured on a stiffer and a softer surface, glass and poly (dimethylsiloxane), respectively. Upon periodic cyclic stimulation of 300 nN at 5 Hz, a significant reduction in the rate of synchronous contraction of the cell patches on poly(dimethylsiloxane) substrates was observed with respect to their spontaneous beat rate, while the cell patches on glass substrates maintained or increased their contraction rate after the stimulation. On the other hand, single cells mostly maintained their contraction rate and could only withstand a lower magnitude of forces compared to micropatterned cell patches. This study reveals that the contraction behavior of cardiomyocytes can be modulated mechanically through cyclic nanomechanical stimulation, and the degree and mode of this modulation depend on the cell connectivity and substrate mechanical properties.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"1849543516675348"},"PeriodicalIF":0.0,"publicationDate":"2016-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849543516675348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36257404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Cross-presentation of lactoferrin encapsulated into chitosan-based nanoparticles. 壳聚糖基纳米颗粒中乳铁蛋白的交叉呈现。

Nanobiomedicine Pub Date : 2016-10-12 eCollection Date: 2016-01-01 DOI: 10.1177/1849543516667355

Tatiana Lyalina, Anastasia Zubareva, Valery Varlamov, Elena Svirshchevskaya

{"title":"Cross-presentation of lactoferrin encapsulated into chitosan-based nanoparticles.","authors":"Tatiana Lyalina, Anastasia Zubareva, Valery Varlamov, Elena Svirshchevskaya","doi":"10.1177/1849543516667355","DOIUrl":"https://doi.org/10.1177/1849543516667355","url":null,"abstract":"Induction of CD8+ cytotoxic T-cell response is essential for the protection from intracellular pathogens. It requires major histocompatibility complex class I processing of newly synthesized proteins transported from the cytosolic pathway. Presentation of mature soluble proteins occurs via a cross-presentation (CP) pathway that is much less efficient in the activation of cytotoxic response. Encapsulation of proteins into polymeric nanoparticles (NPs) can modulate the efficacy of antigen CP. In this article, a model antigen lactoferrin (L) was encapsulated into polysaccharide NPs with different physicochemical properties (size, charge, and hydrophobicity) and used as an immunogen. CD8+ or CD4+ associated IgG2a or IgG1 subclasses of L-specific antibodies, respectively, served as a measure of CD8+ versus CD4+ T-cell activation. Among five types of NPs produced, only succinylchitosan-galactomannan (LSG) and succinylchitosan-PEG-chitosan (LSPC) NPs induced a significant IgG2a response. IgG1 production was comparable in all but hydrophobic succinyl-dodecyl-chitosan (LSD) NPs, where it was only marginal. Confocal studies demonstrated that galactomannan-equipped LSG-NPs induced vacuolar type of CP, while positively charged LSPC-NPs were transported mostly via the cytosolic CP pathway.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"1849543516667355"},"PeriodicalIF":0.0,"publicationDate":"2016-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849543516667355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Microbubble-liposome conjugate: Payload evaluation of potential theranostic vehicle. 微泡-脂质体缀合物:潜在治疗载体的有效载荷评估。

Nanobiomedicine Pub Date : 2016-10-05 eCollection Date: 2016-01-01 DOI: 10.1177/1849543516670806

Ritu Malik, Ketan Pancholi, Andreas Melzer

引用次数: 6

Global Health Innovation Technology Models. 全球卫生创新技术模式。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62921

Kimberly Harding

{"title":"Global Health Innovation Technology Models.","authors":"Kimberly Harding","doi":"10.5772/62921","DOIUrl":"https://doi.org/10.5772/62921","url":null,"abstract":"Chronic technology and business process disparities between High Income, Low Middle Income and Low Income (HIC, LMIC, LIC) research collaborators directly prevent the growth of sustainable Global Health innovation for infectious and rare diseases. There is a need for an Open Source-Open Science Architecture Framework to bridge this divide. We are proposing such a framework for consideration by the Global Health community, by utilizing a hybrid approach of integrating agnostic Open Source technology and healthcare interoperability standards and Total Quality Management principles. We will validate this architecture framework through our programme called Project Orchid. Project Orchid is a conceptual Clinical Intelligence Exchange and Virtual Innovation platform utilizing this approach to support clinical innovation efforts for multi-national collaboration that can be locally sustainable for LIC and LMIC research cohorts. The goal is to enable LIC and LMIC research organizations to accelerate their clinical trial process maturity in the field of drug discovery, population health innovation initiatives and public domain knowledge networks. When sponsored, this concept will be tested by 12 confirmed clinical research and public health organizations in six countries. The potential impact of this platform is reduced drug discovery and public health innovation lag time and improved clinical trial interventions, due to reliable clinical intelligence and bio-surveillance across all phases of the clinical innovation process.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells. 金纳米颗粒撞击核仁和MCF7乳腺癌细胞的应激反应。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62337

Mohamed Kodiha, Hicham Mahboubi, Dusica Maysinger, Ursula Stochaj

{"title":"Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells.","authors":"Mohamed Kodiha, Hicham Mahboubi, Dusica Maysinger, Ursula Stochaj","doi":"10.5772/62337","DOIUrl":"10.5772/62337","url":null,"abstract":"Cancer cells can take up gold nanoparticles of different morphologies. These particles interact with the plasma membrane and often travel to intracellular organelles. Among organelles, the nucleus is especially susceptible to the damage that is inflicted by gold nanoparticles. Located inside the nucleus, nucleoli are specialized compartments that transcribe ribosomal RNA genes, produce ribosomes and function as cellular stress sensors. Nucleoli are particularly prone to gold nanoparticle-induced injury. As such, small spherical gold nanoparticles and gold nanoflowers interfere with the transcription of ribosomal DNA. However, the underlying mechanisms are not fully understood. In this study, we examined the effects of gold nanoparticles on nucleolar proteins that are critical to ribosome biogenesis and other cellular functions. We show that B23/nucleophosmin, a nucleolar protein that is tightly linked to cancer, is significantly affected by gold nanoparticles. Furthermore, gold nanoparticles impinge on the cellular stress response, as they reduce the abundance of the molecular chaperone hsp70 and O-GlcNAc modified proteins in the nucleus and nucleoli. Together, our studies set the stage for the development of nanomedicines that target the nucleolus to eradicate proliferating cancer cells.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Knock-down of ELMO1 in Paediatric Rhabdomyosarcoma Cells by Nanoparticle Mediated siRNA Delivery. 纳米颗粒介导的siRNA递送在小儿横纹肌肉瘤细胞中敲除ELMO1

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62690

Xinyue Huang, Helen Townley

引用次数: 7

ZnO Nanoparticles: A Promising Anticancer Agent. 氧化锌纳米粒子：一种前景广阔的抗癌剂

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/63437

Gunjan Bisht, Sagar Rayamajhi

{"title":"ZnO Nanoparticles: A Promising Anticancer Agent.","authors":"Gunjan Bisht, Sagar Rayamajhi","doi":"10.5772/63437","DOIUrl":"10.5772/63437","url":null,"abstract":"Nanoparticles, with their selective targeting capabilities and superior efficacy, are becoming increasingly important in modern cancer therapy and starting to overshadow traditional cancer therapies such as chemotherapy radiation and surgery. ZnO nanoparticles, with their unique properties such as biocompatibility, high selectivity, enhanced cytotoxicity and easy synthesis, may be a promising anticancer agent. Zinc, as one of the major trace elements of the human body and co-factor of more than 300 mammalian enzymes, plays an important role in maintaining crucial cellular processes including oxidative stress, DNA replication, DNA repair, cell cycle progression and apoptosis. Thus, it is evident that an alteration in zinc levels in cancer cells can cause a deleterious effect. Research has shown that low zinc concentration in cells leads to the initiation and progression of cancer and high zinc concentration shows toxic effects. Zinc-mediated protein activity disequilibrium and oxidative stress through reactive oxygen species (ROS) may be the probable mechanism of this cytotoxic effect. The selective localization of ZnO nanoparticles towards cancer cells due to enhanced permeability and retention (EPR) effect and electrostatic interaction and selective cytotoxicity due to increased ROS present in cancer cells show that ZnO nanoparticles can selectively target and kill cancer cells, making them a promising anticancer agent.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5998263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36256981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aminosilane Functionalization and Cytotoxicity Effects of Upconversion Nanoparticles Y₂O₃ and Gd₂O₃ Co-Doped with Yb³⁺and Er³. Yb3+和Er3共掺杂上转化纳米粒子Y2O3和Gd2O3的氨基硅烷功能化和细胞毒性作用。

Nanobiomedicine Pub Date : 2016-01-01 DOI: 10.5772/62252

Dalia Holanda Chavez, Karla Juarez-Moreno, Gustavo Alonso Hirata

{"title":"Aminosilane Functionalization and Cytotoxicity Effects of Upconversion Nanoparticles Y2O3 and Gd2O3 Co-Doped with Yb3+and Er3.","authors":"Dalia Holanda Chavez, Karla Juarez-Moreno, Gustavo Alonso Hirata","doi":"10.5772/62252","DOIUrl":"https://doi.org/10.5772/62252","url":null,"abstract":"In this study, luminescent upconversion nanoparticles (UCNPs) Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+ were prepared by the sol-gel method (SG). These NPs are able to absorb near infrared photons and upconvert them into visible radiation with a direct application in bioimaging, as an important tool to diagnose and visualize cancer cells. The UCNPs were coated with a thin silica shell and functionalized with amino groups for further folic acid conjugation to allow their interaction with folate ligands on the cell surface. Their physical properties were analysed by Transmission Electron Microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and photoluminescence (PL) measurements. The PL results revealed excellent luminescence properties on all core-shell UCNPs. Cytotoxicity experiments with concentrations of bare and aminosilane coated/functionalized UCNPs between 0.001 μg/mL to 1 μg/mL were tested on two different cell lines from human cervix carcinoma (HeLa) and human colorectal adenocarcinoma (DLD-1) with a colorimetric assay based on the reduction of MTT reagent (methy-134-thiazolyltetrazolium). The assays show that some concentrations of bare UCNPs were cytotoxic for cervical adenocarcinoma cells (HeLa); however, for human colorectal adenocarcinoma all UCNPs are non-cytotoxic. After UCNPs functionalization with silica-aminosilane (APTES/TEOS), all of the nanoparticles tested were found to be non-cytotoxic for both cell lines. The UCNPs functionalized in this work can be further conjugated with specific ligands and used as biolabels for detection of cancer cells.","PeriodicalId":56366,"journal":{"name":"Nanobiomedicine","volume":"3 ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36257594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28