Japanese Journal of Veterinary Research最新文献_第8页

Salmonella serovars and antimicrobial resistance in strains isolated from wild animals in captivity in Sinaloa, Mexico. 从墨西哥锡那罗亚圈养野生动物中分离出的沙门氏菌血清型和抗微生物药物耐药性。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2014-08-01

Gabriela Silva-Hidalgo, Martin López-Valenzuela, Felipe Juárez-Barranco, Edith Montiel-Vázquez, Beatriz Valenzuela-Sánchez

引用次数: 0

Contamination status and possibility of toxic effects of co-planar polychlorinated biphenyls, polycyclic aromatic hydrocarbons, and dichlorodiphenyltrichloroethane in large japanese field mouse (Apodemus speciosus) collected from Hokkaido and Aomori. 北海道和青森地区日本大田鼠共面多氯联苯、多环芳烃和二氯二苯三氯乙烷的污染现状及毒性作用可能性

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2014-08-01

Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kentaro Q Sakamoto, Shoichi Fujita, Mayumi Ishizuka

{"title":"Contamination status and possibility of toxic effects of co-planar polychlorinated biphenyls, polycyclic aromatic hydrocarbons, and dichlorodiphenyltrichloroethane in large japanese field mouse (Apodemus speciosus) collected from Hokkaido and Aomori.","authors":"Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kentaro Q Sakamoto, Shoichi Fujita, Mayumi Ishizuka","doi":"","DOIUrl":"","url":null,"abstract":"Contamination levels of coplanar polychlorinated biphenyls (Co-PCBs), polycyclic aromatic hydrocarbons (PAHs), and dichlorodiphenyltrichloroethane (DDTs) were measured in the entire body of the large Japanese field mouse (Apodemus speciosus) collected from Hokkaido (Ishikari and Rankoshi) and Aomori prefecture (Takko) in Japan. Higher concentrations of PCBs including Co-PCBs, were observed in the mice collected from Ishikari than those from Rankoshi. The concentration of PAHs in the soil from Ishikari was also higher than that in the other sampling sites. The findings suggest that Ishikari is the most polluted area, probably because of human activities, depending on the population distribution. However, the observed contaminant levels were extremely lower compared to those in previous studies. The ratio of testis weight to body weight (TW/BW) was the lowest in the mice collected from Ishikari, which is the area contaminated with PAHs and p,p'-dichlorodiphenyldichloroethylene (DDE). However, the serum testosterone levels of mice from the Ishikari area were higher than those from the non-contaminated other areas although no significant differences. Previous studies have shown that a low-level exposure to dioxin related compounds (DRCs) disturbances in sexual function, resulting in the production of testosterone. This study showed that POPs exposure is one of the possibility of the high testosterone concentration in the mice of the Ishikari area in addition to a cause of biological and environmental factors such as habitat density, age, temperatures and/or food riches.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32719388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin. 东方芽孢杆菌23kda肝质膜蛋白与肝素的相互作用。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2014-05-01

Hitoshi Takemae, Tatsuki Sugi, Kyousuke Kobayashi, Fumi Murakoshi, Frances C Recuenco, Akiko Ishiwa, Atsuko Inomata, Taisuke Horimoto, Naoaki Yokoyama, Kentaro Kato, Kentaro Kato

引用次数: 0

Measurement of M-mode echocardiographic parameters in healthy adult Van cats. 健康成年Van猫m型超声心动图参数的测定。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2014-05-01

Abdullah Kayar, Cumali Ozkan, Onur Iskefli, Abdullah Kaya, Suleyman Kozat, Yakup Akgul, Remzi Gonul, Mehmet Erman Or

{"title":"Measurement of M-mode echocardiographic parameters in healthy adult Van cats.","authors":"Abdullah Kayar, Cumali Ozkan, Onur Iskefli, Abdullah Kaya, Suleyman Kozat, Yakup Akgul, Remzi Gonul, Mehmet Erman Or","doi":"","DOIUrl":"","url":null,"abstract":"Cardiomyopathies are the most common type of cardiac diseases in cats. Although some normal echocardiographic values for cats have been published, there are variations based on breeds and gender. The objective of this study is to determine normal reference values for M-mode echocardiographic parameters in nonsedated healthy adult Van cats and to compare those values with data reported for nonsedated healthy cats of other breeds. A total of 40 clinically healthy Van cats of both sexes belonging to the Van Cat Research and Application Center of Yuzuncu Yil University were used. Body weight (BW) and 16 M-mode echocardiographic variables were measured in 40 healthy Van cats. The effect of gender and age on each echocardiographic parameter was analyzed and the relationship between BW and each parameter investigated. There was a significant relationship between gender and left atrial dimension during ventricular systole (LAD) and aortic root dimension at end-diastole (AOD) as well as between BW and interventricular septal thickness at end-diastole (IVSd) and end-systole (IVSs), left ventricular internal dimension at end-diastole (LVIDd), left ventricular posterior wall thickness at end-diastole (LVPWd), LAD, AOD, the left ventricular end diastolic volume (EDV) and the stroke volume (SV). A relationship between age and the SV parameter alone was also established. This present study is the first work on cardiac reference values for Van cats highlighting the differences in some M-mode echocardiographic parameters of healthy adult Van cats and other cat breeds, which should be considered when interpreting echocardiographic findings, in order to draw the correct conclusions regarding cardiac health.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32467646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and antimicrobial resistance of Salmonella isolated from poultry slaughterhouses in Korea. 韩国家禽屠宰场分离沙门氏菌的流行和耐药性。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-11-01

Dessie Hirut Kidie, Dong Hwa Bae, Young Ju Lee

{"title":"Prevalence and antimicrobial resistance of Salmonella isolated from poultry slaughterhouses in Korea.","authors":"Dessie Hirut Kidie, Dong Hwa Bae, Young Ju Lee","doi":"","DOIUrl":"","url":null,"abstract":"We determined the antimicrobial resistance of Salmonella serovars from a total of 154 (44 chilling waters and 110 carcasses) samples collected from 22 poultry slaughterhouses. Standard culture techniques, Kauffmann-White slide agglutination and disc diffusion tests were used to isolate, and identify the serovars and to assess the antimicrobial activity, respectively. A total of 88 isolates belonging to 34 Salmonella serovars from 67 (43.5%) positive samples were identified. Among the samples examined, 68.2% (15/22), 22.7% (5/22), and 42.7% (47/110) from the first chilling waters, the last chilling waters, and carcasses were found contaminated with Salmonella, respectively. The prevalent serovars were S. Enteritidis (12.5%) followed by S. Montevideo and S. Senftenberg (8.0%). Rare Salmonella serovars such as S. Aba, S. Malmoe, S. Westhampton, S. Takoradi, and S. Baiboukoum in chicken slaughterhouses and S. Newbrunswick, S. Huddinge S. Glostrup, S. Dujugu, S. Goettingen and S. II in duck slaughterhouses were also detected. Among the serovars, 52.3% (46/88) and 21.6% (19/88) were resistant to one antibiotic and more than two antibiotics, respectively. High antimicrobial resistance rates against sulfamethoxazole (39.8%) followed by tetracycline (22.7%), nalidixic acid (21.6%), ampicillin and amoxicillin-clavulanic acid (8.0%), and chloramphenicol (4.5%) were observed. These results suggest more stringent hygienic measures should be taken to reduce the incidence of pathogen contamination in the food chain.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32012517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and characterization of cathepsin B from tsetse (Glossina morsitans morsitans). 舌蝇组织蛋白酶B的表达与特性研究。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-11-01

Ngasaman Ruttayaporn, Mo Zhou, Keisuke Suganuma, Shino Yamasaki, Shin-ichiro Kawazu, Noboru Inoue

{"title":"Expression and characterization of cathepsin B from tsetse (Glossina morsitans morsitans).","authors":"Ngasaman Ruttayaporn, Mo Zhou, Keisuke Suganuma, Shino Yamasaki, Shin-ichiro Kawazu, Noboru Inoue","doi":"","DOIUrl":"","url":null,"abstract":"Digestive enzymes in tsetse fly midgut are thought to modulate the development of African trypanosome which is a causative agent of trypanosomosis in human and animal. Cathepsin B is induced after the first blood meal ingestion and being higher in trypanosome infected flies. A DNA fragment encoding pro-cathepsin B (930 bp) (Accession No. AF329480_1) was cloned and expressed in E. coli and P. pastoris protein expression systems. An active recombinant cathepsin B (rGmcathB) produced by P. pastoris was migrating from 35 to 45 kDa under reducing condition, rGmcathB exhibited the highest proteolytic activity at pH 4.0 with wide range temperature 25-30 degrees C, also degraded bovine hemoglobin and serum albumin. rGmcathB exhibited hydrolysis preference for Z-Arg-Arg-MCA (K(cat)/K(M) 7.58 mM(-1)sec(-1)) and bovine hemoglobin (K(cat)/K(M) 3.77 x 10(3) mM(-1)sec(-1)). The proteolytic activity of rGmcathB was inhibited by specific cysteine protease inhibitor (E-64) confirmed belonging to papain-like cysteine protease family. These results indicated that rGmcathB shows the activity of cathepsin B and have high affinity with blood protein referring a role in blood meal digestion. In this study, the recombinant protein expressed by E. coli expression system was not enzymatically active as shown in the recombinant protein expressed by P. pastoris expression system. This finding implies that P. pastoris expression system is more suitable for expressing enzymatically active recombinant proteases than E. coli expression system.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2013-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32012518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate. 牛卵母细胞单株培养的体外成熟系统。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-11-01

Masashi Nagano, Sung-Sik Kang, Keisuke Koyama, Weiping Huang, Yojiro Yanagawa, Yoshiyuki Takahashi

引用次数: 0

Limited inhibitory effects of non-steroidal antiinflammatory drugs on in vitro osteogenic differentiation in canine cells. 非甾体类抗炎药对犬细胞体外成骨分化的抑制作用有限。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-08-01

Namgil Oh, Takafumi Sunaga, Hiroki Yamazaki, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura

{"title":"Limited inhibitory effects of non-steroidal antiinflammatory drugs on in vitro osteogenic differentiation in canine cells.","authors":"Namgil Oh, Takafumi Sunaga, Hiroki Yamazaki, Kenji Hosoya, Satoshi Takagi, Masahiro Okumura","doi":"","DOIUrl":"","url":null,"abstract":"Cyclooxygenase (COX)-2 participates essentially in bone healing, demonstrated by COX-2 knockout mice that showed delayed fracture repair. Considerable controversy still exists on inhibitory effects of COX-2 inhibitors on bone healing in clinical cases. To assess stage-dependent effects of short-term treatment of COX-2 inhibitors on osteogenic differentiation, a canine POS osteosarcoma cell line which spontaneously differentiates into osteoblastic cell was exposed to COX-2 inhibitors such as carprofen and meloxicam for 72 hours during three different stages of osteoblast differentiation, including day 0 to 3 (pre-osteoblastic stage), day 4 to 7 (transitional stage) and day 8 to 11 (mature osteoblastic stage). As osteogenic markers, expression of alkaline phosphatase (ALP) was estimated by analysis of mRNA expression, enzymatic activity and ALP staining, and expression of osteocalcin was estimated by analysis of mRNA expression after the drug treatments. Calcified matrix formation was finally observed by von Kossa staining on day 14. Expressions of ALP showed no significant suppression by carprofen and meloxicam during all three stages. However, expressions of osteocalcin mRNA and non-calcified nodule formations were delayed by carprofen and meloxicam during transitional stage. Nevertheless, fully calcified nodule formation was observed in all experimental groups during post-medication period. These results indicate that short-term treatment of carprofen and meloxicam would reversibly suppress the differentiation of osteoblasts.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31751898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibodies to H5 subtype avian influenza virus and Japanese encephalitis virus in northern pintails (Anas acuta) sampled in Japan. 日本北部尖尾鱼H5亚型禽流感病毒和日本脑炎病毒抗体样本

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-08-01

Andrew M Ramey, Erica Spackman, Jung-Yong Yeh, Go Fujita, Kan Konishi, Kiyoshi Uchida, John A Reed, Benjamin R Wilcox, Justin D Brown, David E Stallknecht

引用次数: 0

C-X-C chemokine receptor type 4 and cytokine expressions in cows of a dairy herd with high prevalence of calves persistently infected with bovine viral diarrhea virus. 牛病毒性腹泻病毒持续感染高发奶牛群中C-X-C趋化因子受体4型和细胞因子的表达

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2013-08-01

Mahmoud Atef Youssef Helal, Yuki Hoshino, Satoshi Takagi, Motoshi Tajima

{"title":"C-X-C chemokine receptor type 4 and cytokine expressions in cows of a dairy herd with high prevalence of calves persistently infected with bovine viral diarrhea virus.","authors":"Mahmoud Atef Youssef Helal, Yuki Hoshino, Satoshi Takagi, Motoshi Tajima","doi":"","DOIUrl":"","url":null,"abstract":"Animals persistently infected (PI) with bovine viral diarrhea virus (BVDV) play an important role in the spread of BVDV. Alteration of maternal C-X-C chemokine receptor type 4 (CXCR4) expression has been suspected as closely concerned with the production of PI calves. It is not clear what the influence of CXCR4 response to the prevalence of PI calves. We have previously reported a dairy herd with high prevalence of PI calves within a short period having a single origin of infection. CXCR4 and cytokine expressions in cows of this herd were investigated. There were no significant differences in CXCR4 and cytokine expressions between the dams of PI calves and the dams of non PI calves in the herd. In the comparison among the herds, CXCR4 expressions in the PI producing herds were significantly lower than the BVDV-free herd. Moreover, CXCR4 expressions in the high prevalence herd and the low prevalence herd were similar. These findings among herds corresponded with the previously reported experimental production of persistent infection with BVDV in cows. Based on the cytokine profile of these herds, IL-10 was significantly higher in the high prevalence herd and the BVDV-free herd. The combination of low expression of CXCR4 and high expression of IL-10 might be closely concerned with some bias for the production of PI calves.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31751899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0