Japanese Journal of Veterinary Research最新文献

Inactivation of sars-cov-2 by povidone-iodine products: Implications for effective mouth rinsing and gargling 聚维酮碘产品灭活sars-cov-2:对有效漱口和漱口的影响

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2021-08-01 DOI: 10.14943/jjvr.69.3.183

H. Kariwa, H. Sawa, Shintaro Kobayashi

引用次数: 4

Can procalcitonin be used as a clinical biomarker during bacterial, viral and parasitic infections in dogs? 降钙素原能否作为犬类细菌、病毒和寄生虫感染的临床生物标志物?

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2021-02-01 DOI: 10.14943/JJVR.69.1.5

E. Matur, B. Dokuzeylül, Mukaddes Özcan, H. Çetinkaya, Murat Arslan, E. Or, Songül Erhan, Ü. Çötelioğlu

{"title":"Can procalcitonin be used as a clinical biomarker during bacterial, viral and parasitic infections in dogs?","authors":"E. Matur, B. Dokuzeylül, Mukaddes Özcan, H. Çetinkaya, Murat Arslan, E. Or, Songül Erhan, Ü. Çötelioğlu","doi":"10.14943/JJVR.69.1.5","DOIUrl":"https://doi.org/10.14943/JJVR.69.1.5","url":null,"abstract":"The aim of this study is to investigate the usability of serum procalcitonin level in dogs as a clinical biomarker for the distinction between bacterial, viral, and parasitic diseases. A total of 160 dogs were used. The animals were evaluated in four groups as control and those with bacterial, viral, and parasitic infections. Serum procalcitonin, tumor necrosis factor-alpha (TNF- α ), interleukin-1 beta (IL-1 β ), interleukin-6 (IL-6), interferon-gamma (IFN- γ ) level, and total white blood cell (WBC), and differential leukocyte count were determined. Serum procalcitonin level was found to be higher in dogs with bacterial infection compared to the control group and dogs with viral disease ( P = 0.019). It was observed that serum procalcitonin level in dogs with bacterial infection varies related to the disease agent and it was found higher in those with pyometra ( P = 0.009). Serum procalcitonin level was higher in dogs which had parasitic infections but, the difference is not significant. IL-6 level was found higher in dogs with viral and parasitic diseases than those in the control ( P = 0.006). A negative correlation was determined between procalcitonin and IFN- γ ( P = 0.001). While a positive correlation was detected between procalcitonin and WBC count, a negative correlation was determined between procalcitonin and monocyte percentage. In conclusion, serum procalcitonin level can be used as a clinical biomarker in bacterial diseases and, perhaps, in some parasitic diseases in dogs. However, further studies should be conducted to determine threshold values that take the severity of infection, its prevalence, and clinical course into account.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41285184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1) 小隐孢子虫1型病毒高灵敏检测方法的建立

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2020-08-01 DOI: 10.14943/JJVR.68.3.159

A. Shehata, H. Bando, Y. Fukuda, Mohammad Hazzaz Bin Kabir, F. Murakoshi, M. Itoh, A. Fujikura, Hiroaki Okawa, Takuto Endo, A. Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, K. Otomaru, Kei Kazama, M. Essa, Kentaro Kato

{"title":"Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1)","authors":"A. Shehata, H. Bando, Y. Fukuda, Mohammad Hazzaz Bin Kabir, F. Murakoshi, M. Itoh, A. Fujikura, Hiroaki Okawa, Takuto Endo, A. Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, K. Otomaru, Kei Kazama, M. Essa, Kentaro Kato","doi":"10.14943/JJVR.68.3.159","DOIUrl":"https://doi.org/10.14943/JJVR.68.3.159","url":null,"abstract":"Cryptosporidium is an apicomplexan zoonotic parasite that infects most mammals, including humans. Cryptosporidium parvum virus type 1 (CSpV1) is the first member within the Partitiviridae family recognized to infect protozoan hosts. Cryptosporidium tracking based on CSpV1 detection has been attempted; however, each study used different conditions for the PCR protocol, primers, and target viral sequences. Accordingly, the sensitivity of PCR-based CSpV1 detection remains unclear. In addition, oocyst purification from clinical samples can be problematic due to small number of oocysts, sample degradation and low yield efficiency of currently used purification methods. Here we show that the second half of the coding region of dsRNA2 can be detected from various types of clinical samples, without the need for oocyst purification, by using a semi-nested-PCR technique. Furthermore, we show that the short sequence targeted in this study has higher diversity than the Cryptosporidium GP60 gene. Taken together, our findings suggest that this method could be used as an important tracking marker for Cryptosporidium species.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49036318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Clinical efficacy of the combined treatment of anti-PD-L1 rat-bovine chimeric antibody with a COX-2 inhibitor in calves infected with Mycoplasma bovis 抗pd - l1大鼠-牛嵌合抗体联合COX-2抑制剂治疗牛支原体感染犊牛的临床疗效

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2020-05-01 DOI: 10.14943/JJVR.68.2.77

S. Goto, S. Konnai, Yuki Hirano, J. Kohara, T. Okagawa, N. Maekawa, Y. Sajiki, Kei Watari, Erina Minato, Atsuhi Kobayashi, S. Gondaira, H. Higuchi, M. Koiwa, M. Tajima, E. Taguchi, M. Ishida, R. Uemura, S. Yamada, M. Kaneko, Y. Kato, Keiichi Yamamoto, Mikihiro Toda, Yasuhiko Suzuki, S. Murata, K. Ohashi

引用次数: 8

NMR based serum metabolomics for monitoring newborn preterm calves'health 基于核磁共振的血清代谢组学监测新生儿早产儿的健康状况

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2020-05-01 DOI: 10.14943/JJVR.68.2.105

A. Basoglu, N. Başpınar, Cristina Licari, L. Tenori, A. Naseri

{"title":"NMR based serum metabolomics for monitoring newborn preterm calves'health","authors":"A. Basoglu, N. Başpınar, Cristina Licari, L. Tenori, A. Naseri","doi":"10.14943/JJVR.68.2.105","DOIUrl":"https://doi.org/10.14943/JJVR.68.2.105","url":null,"abstract":"It was aimed to detect the novel future biomarkers using a metabolomics approach in premature calves. Calves born previous to 270 days' pregnancy are at risk, and the earlier the calving is, the higher the risk. More trials are needed in neonatology field as it little known almost the generally metabolic status of preterm neonates. To date, this is the first NMR (nuclear magnetic resonance) based study on serum metabolomics at set intervals in premature calves. Biochemical health profiles and NMR based metabolomic analysis were performed in twenty-five premature dairy calves. The whole animals partly recovered following 72h. Clinical data were compatible with those of premature animals. Increased levels of AST and CPK may be attributed to subclinic trauma at birth. Alterations in metabolites, increases in 3-hydroxybutyrate, citrate, leucine and isoleucine at 48th and 72h; choline, formate, fatty acids and polyunsaturated fatty acids at 72h, and valine at 48h; and decreases in myo-inositol at 48h and 72h were meaningful for monitoring the recovery at a molecular level in premature calves. Metabolomics became an important tool for identification of premature calves’ clinical pathology and monitoring therapeutic picture.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48391673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on counter measures to coronavirus disease 2019 (COVID-19) pandemic, May 2020 2019冠状病毒病(COVID-19)大流行应对措施综述，2020年5月

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2020-01-01 DOI: 10.14943/JJVR.68.3.133

Taishi Kidaka, S. Lokupathirage, B. D. S. Muthusinghe, B. Lombe Pongombo, Christida E Wastika, Zhouxing Wei, Shizuka Yoshioka, M. Ishizuka, Y. Sakoda, H. Kariwa, N. Isoda

{"title":"Review on counter measures to coronavirus disease 2019 (COVID-19) pandemic, May 2020","authors":"Taishi Kidaka, S. Lokupathirage, B. D. S. Muthusinghe, B. Lombe Pongombo, Christida E Wastika, Zhouxing Wei, Shizuka Yoshioka, M. Ishizuka, Y. Sakoda, H. Kariwa, N. Isoda","doi":"10.14943/JJVR.68.3.133","DOIUrl":"https://doi.org/10.14943/JJVR.68.3.133","url":null,"abstract":"An outbreak of novel coronavirus infection occurred in China at the end of 2019, which was designated as coronavirus disease 2019 (COVID-19), and spread to regions across Asia and ultimately all over the world As of 21 May 2020, a total of more than 5 million cases with more than 350 thousand deaths were reported worldwide Evaluation of the pathogenicity of the disease and determining the efficacy of control measures are essential for rapid containment of the disease However, the world is facing difficulties in controlling COVID-19 at both of the national and global levels due to variations in pathogenicity of infection by severe acute respiratory syndrome coronavirus 2, the causal agent of COVID-19, and to diverse measures applied in each country based on their control capacities and policies In the present review, we summarize the basic information and findings related to the COVID- 19 pandemic, including pathogen agent, epidemiology, disease transmission, and clinical manifestations Diagnosis, treatment, and preventive measures applied or under development all over the world are also reviewed to provide the opportunity to establish a more effective scenario for disease containment Humanity has progressed by developing countless great technologies and immense scientific theories, however it may be a fact that we cannot conquer all risks to humanity New findings and challenges for the unprecedented pandemic at the global level, such as COVID-19, should also contribute to preparedness for unknown diseases in future, similar to the lessons learnt from severe acute respiratory syndrome and the pandemic A(H1N1)pdm09 influenza","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66736354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan 从日本猪粪便中发现免疫缺陷相关病毒样病毒的基因组

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2018-01-01 DOI: 10.14943/JJVR.66.1.53

M. Oba, Y. Katayama, S. Tsuchiaka, T. Omatsu, Yoshiteru Murata, K. Ohya, S. Makino, M. Nagai, T. Mizutani

引用次数: 2

Signal changes in standing magnetic resonance imaging of septic pedal osteitis in a thoroughbred racehorse 纯种赛马脓毒性足部骨炎的站立磁共振成像信号变化

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2017-05-01 DOI: 10.14943/JJVR.65.2.101

Fumiaki Mizobe, M. Nomura, Tomohiro Kato, Yoshinari Katayama, A. Kuwano, T. Ueno, Kazutaka Yamada, M. Spriet

引用次数: 0

Identification of novel alternative splicing variants within swine Setd8 gene and their high mRNA expression in testis. 猪Setd8基因新的选择性剪接变异的鉴定及其在睾丸中的高mRNA表达。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2017-02-01

Shuai Yu, Xiaoxu Chen, Zhongyuan Deng, Xianyong Lan, Chuanying Pan

{"title":"Identification of novel alternative splicing variants within swine Setd8 gene and their high mRNA expression in testis.","authors":"Shuai Yu, Xiaoxu Chen, Zhongyuan Deng, Xianyong Lan, Chuanying Pan","doi":"","DOIUrl":"","url":null,"abstract":"SET domain containing (lysine methyltransferase) 8 (Setd8), a histone modification enzyme, affects cell cycling, chromosome condensation, high efficient repair of DNA double strand breaks and so on. The objective of this study was to identify novel alternative splicing variants of pig Setd8 gene and its mRNA expression. Four 180-day-old male Guanzhong Black (GZB) pigs and six male Landrace piglets (including three 30-day-old and three 7-day-old pigs) were collected to study Setd8 gene. Herein, two novel variants, Setd8a and Setd8b, were found in pig. The entire sequences of Setd8a and Setd8b variants were 1,039 bp and 958 bp, respectively. qRT-PCR results showed that Setd8a and Setd8b were highly expressed in brains and testes of 180-day-old GZB pigs. Moreover, the expressions of the two Setd8 variants were significantly higher in testis than brain of GZB pig (P < 0.05). Further study on testis showed that the mRNA expression of Setd8a variant was significantly lower than Setd8b variant in 30-day-old and 7-day-old pigs (P < 0.05). The mRNA expression of Setd8a variant'was lower than Setd8b variant in GZB pigs (P > 0.05). Moreover, the expressions of the two Setd8 variants were significantly higher along with age enlargement. In conclusion, Setd8a and Setd8b were firstly identified in pigs and both were expressed in pig testis. Setd8b was the major splicing variant of pig Seid8 gene transcript product. Moreover, the expressions of Setd8 variants were time-dependent. All these findings would enrich the study of Setd8 gene in pig testis.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36123844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-neoplastic effects of topoisomerase inhibitors in canine mammary carcinoma, melanoma, and osteosarcoma cell lines. 拓扑异构酶抑制剂在犬乳腺癌、黑色素瘤和骨肉瘤细胞系中的抗肿瘤作用。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2017-02-01

Siew Mei Ong, Hiroki Yamamoto, Kohei Saeki, Yuiko Tanaka, Ryohei Yoshitake, Ryohei Nishimura, Takayuki Nakagawa

{"title":"Anti-neoplastic effects of topoisomerase inhibitors in canine mammary carcinoma, melanoma, and osteosarcoma cell lines.","authors":"Siew Mei Ong, Hiroki Yamamoto, Kohei Saeki, Yuiko Tanaka, Ryohei Yoshitake, Ryohei Nishimura, Takayuki Nakagawa","doi":"","DOIUrl":"","url":null,"abstract":"Numerous topoisomerase inhibitors with proven efficacy have been used extensively to treat various human neoplasms. However, among these, only doxorubicin has been used and studied extensively in veterinary oncology. The current study was performed to evaluate the responsiveness of canine osteosarcoma (cOSA), mammary gland tumour (cMGT), and malignant melanoma (cMM) cell lines to several topoisomerase inhibitors. In addition, the correlation between the sensitivity to treatment and multi-drug resistant (MDR) factors was investigated. cOSA cell lines exhibited higher sensitivity than cMGT and cMM cell lines to all the topoisomerase inhibitors tested in vitro; this was associated with the levels of multi-drug resistance protein 1 (MDR1) gene expression in the cOSA cell lines. Treatment of cOSA (HMPOS) and cMGT cell line (CHMp) xenograft mouse models with etoposide markedly delayed tumour progression in HMPOS xenografts, but failed to elicit lasting anti-tumour effects on CHMp xenograft mice. The present findings suggest that MDR1 represents a molecular signature for prediction of treatment efficacy of topoisomerase inhibitors, especially that of etoposide, which may be a clinically useful anti-tumour agent for cOSA; however, further study is necessary to refine the treatment protocol.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36123845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0