Japanese Journal of Veterinary Research最新文献_第6页

Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspension and forced swimming tests. 小鼠悬尾和强制游泳试验中控制不动时间的多个基因位点的鉴定。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-05-01

Ahmed F Abou-Elnaga, Daisuke Torigoe, Mohamed M Fouda, Ragab A Darwish, Usama A Abou-Ismail, Masami Morimatsu, Takashi Agui

{"title":"Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspension and forced swimming tests.","authors":"Ahmed F Abou-Elnaga, Daisuke Torigoe, Mohamed M Fouda, Ragab A Darwish, Usama A Abou-Ismail, Masami Morimatsu, Takashi Agui","doi":"","DOIUrl":"","url":null,"abstract":"Depression is one of the most famous psychiatric disorders in humans in all over the countries and considered a complex neurobehavioral trait and difficult to identify causal genes. Tail suspension test (TST) and forced swimming test (FST) are widely used for assessing depression-like behavior and antidepressant activity in mice. A variety of antidepressant agents are known to reduce immobility time in both TST and FST. To identify genetic determinants of immobility duration in both tests, we analyzed 101 F2 mice from an intercross between C57BL/6 and DBA/2 strains. Quantitative trait locus (QTL) mapping using 106 microsatellite markers revealed three loci (two significant and one suggestive) and five suggestive loci controlling immobility time in the TST and FST, respectively. Results of QTL analysis suggest a broad description of the genetic architecture underlying depression, providing underpinnings for identifying novel molecular targets for antidepressants to clear the complex genetic mechanisms of depressive disorders.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34280953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Class 1 integrons characterization and multilocus sequence typing of Salmonella spp. from swine production chains in Chiang Mai and Lamphun provinces, Thailand. 泰国清迈和兰埔省猪生产链沙门氏菌1类整合子特征及多位点序列分型

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-05-01

Phacharaporn Boonkhot, Pakpoom Tadee, Panuwat Yamsakul, Chairoj Pocharoen, Nipa Chokesajjawatee, Prapas Patchanee

{"title":"Class 1 integrons characterization and multilocus sequence typing of Salmonella spp. from swine production chains in Chiang Mai and Lamphun provinces, Thailand.","authors":"Phacharaporn Boonkhot, Pakpoom Tadee, Panuwat Yamsakul, Chairoj Pocharoen, Nipa Chokesajjawatee, Prapas Patchanee","doi":"","DOIUrl":"","url":null,"abstract":"Pigs and pork products are well known as an important source of Salmonella, one of the major zoonotic foodborne pathogens. The emergence and spread of antimicrobial resistance is becoming a major public health concern worldwide. Integrons are genetic elements known to have a role in the acquisition and expression of genes conferring antibiotic resistance. This study focuses on the prevalence of class 1 integrons-carrying Salmonella, the genetic diversity of strains of those organisms obtained from swine production chains in Chiang Mai and Lamphun provinces, Thailand, using multilocus sequence typing (MLST) and comparison of genetic diversity of sequence types of Salmonella from this study with pulsotypes identified in previous study. In 175 Salmonella strains, the overall prevalence of class 1 integrons-carrying-Salmonella was 14%. The gene cassettes array pattern \"dfrA12-orfF-aadA2\" was the most frequently observed. Most of the antimicrobial resistance identified was not associated with related gene cassettes harbored by Salmonella. Six sequence types were generated from 30 randomly selected strains detected by MLST. Salmonella at the human-animal-environment interface was confirmed. Linkages both in the farm to slaughterhouse contamination route and the horizontal transmission of resistance genes were demonstrated. To reduce this problem, the use of antimicrobials in livestock should be controlled by veterinarians. Education and training of food handlers as well as promotion of safe methods of food consumption are important avenues for helping prevent foodborne illness.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34280956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant status, metabolic profile and immune response of lambs supplemented with tannin rich Ficus infectoria leaf meal. 添加富含单宁的榕叶粕对羔羊抗氧化状态、代谢特征和免疫反应的影响。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Avijit Dey, Narayan Dutta, Ashok Kumar Pattanaik, Kusumakar Sharma

{"title":"Antioxidant status, metabolic profile and immune response of lambs supplemented with tannin rich Ficus infectoria leaf meal.","authors":"Avijit Dey, Narayan Dutta, Ashok Kumar Pattanaik, Kusumakar Sharma","doi":"","DOIUrl":"","url":null,"abstract":"To study the effect of supplementation of tanniferous tree leaves Ficus infectoria on antioxidant status and immune response, twenty four lambs were randomly divided into four groups of six each in a completely randomized design and fed either a conventional supplement (CON) or experimental supplements (FILM-I, FILM-II and FILM-III) containing 1.0, 1.5 and 2.0% condensed tannins (CT), respectively by replacement of wheat bran of supplement CON with Ficus infectoria leaf meal (FILM). Blood biochemical profile was monitored in all lambs at 0, 45, 90, 135, 180 days of feeding. Although haemato-biochemical parameters remained similar, there was significant (p < 0.05) improvement in catalase activity, total thiol and protein thiol groups with reduction in lipid peroxidation (LPO) in lambs fed FILM diet irrespective of levels. However, intracellular status of reduced glutathione, and superoxide dismutase activity was improved (p < 0.05) only in FILM-II and FILM-III supplemented lambs. The cell-mediated immune response was significantly (p < 0.05) improved in all the lambs fed FILM supplemented diets. Improved antioxidant status and immunity in FILM supplemented lambs increased voluntary feed intake irrespective of level. However, the average daily gain for a period of 180 d showed a significant (p < 0.05) increase by the supplementation of FILM-II diet containing 1.5% CT. The present study reveals that the supplementation of Ficus infectoria leaf meal up to 21.2% in the concentrate mixture could improve the antioxidant status and immunity in lambs. However, as feed efficiency was reduced at higher levels due to presence of CT, 15.9% supplementation containing 1.5% condensed tannins in concentrate mixture is suggested to improve the health and production performance of lambs.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33200028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quadricuspid aortic valves in Syrian hamsters and their formation according to current knowledge on valvulogenesis. 叙利亚仓鼠的四尖瓣主动脉瓣及其形成。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Alejandro López-García, M Carmen Fernández, Ana Carmen Durán, Valentín Sans-Coma, Borja Fernández

{"title":"Quadricuspid aortic valves in Syrian hamsters and their formation according to current knowledge on valvulogenesis.","authors":"Alejandro López-García, M Carmen Fernández, Ana Carmen Durán, Valentín Sans-Coma, Borja Fernández","doi":"","DOIUrl":"","url":null,"abstract":"Occurrence of quadricuspid aortic valves has been reported in humans, in nine dogs and in a greater white-toothed shrew. Moreover, two cases of developing aortic valves with four anticipated leaflets have been described in Syrian hamster embryos. Currently, however, no case of quadricuspid aortic valve in adult hamsters has been recorded. The aim here is to present four adults of this rodent species, two of them with unequivocally quadricuspid aortic valves and the other two with quadricuspid-like aortic valves. The four anomalous aortic valves were detected among 4,190 Syrian hamsters examined in our laboratory, representing an incidence of 0.09%. None of the affected hamsters showed apparent signs of disease. The present findings are considered on the light of current empirical knowledge about the morphogenesis of quadricuspid and bicuspid aortic and pulmonary valves. Quadricuspid aortic valves result from the partition of one of the normal mesenchymal cushions which normally give rise to normal (tricuspid) valves, while quadricuspid-like valves might be the product of a combined mechanism of fusion and partition of the cushions at the onset of the valvulogenesis. The presence of aortic valves with four leaflets in ancient mammalian lineages such as insectivors and rodents suggest that quadricuspid aortic valves, although showing almost certainly a low incidence, may be widespread among the different groups of mammals, including domestic animals.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33200030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diagnosis of band 3 deficiency using a quenching probe (QProbe)-PCR assay in bovine embryos. 用猝灭探针(QProbe)-PCR方法诊断牛胚胎带3缺陷。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Soichi Kageyama, Hiroki Hirayama, Satoru Moriyasu, Akira Minamihashi

{"title":"Genetic diagnosis of band 3 deficiency using a quenching probe (QProbe)-PCR assay in bovine embryos.","authors":"Soichi Kageyama, Hiroki Hirayama, Satoru Moriyasu, Akira Minamihashi","doi":"","DOIUrl":"","url":null,"abstract":"The present study was conducted to develop a simple and rapid procedure to determine the genotype of band 3 deficiency in bovine embryos by a novel real-time PCR method using a fluorescent quenching-based probe (QProbe-PCR). QProbe-PCR successfully distinguished wild type and R664X mutant alleles by melting curve analysis. Minimal amounts of DNA template were required for the detection of wild type/wild type alleles, mutant/mutant alleles, and wild type/mutant alleles; their amounts were 10 pg, 25 pg, and 50 pg, respectively. When 10 blastomeres were used as a DNA sample, accuracies of genotyping by QProbe-PCR were 100% and 89% in embryos homozygous for the wild type allele and heterozygous for the wild type and mutant alleles, respectively. QProbe-PCR takes approximately 2 h for genotyping and requires lesser time than the conventional method using PCR-RFLP, which requires digestion with a restriction enzyme and electrophoresis. Our data showed that QProbe-PCR is a useful method for rapid analysis of the genetic deficiency in preimplantation embryos. Reduction in the time required for genotyping enabled the transfer of genetically selected embryos to recipient cows on the day of embryo collection. These results suggest that determination of the genotype for the genetic deficiency in embryos is useful to select animals free from the genetic disease, and it also makes it possible to produce an animal model homozygous for the mutation.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33199956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction between metabolic challenges and productivity in high yielding dairy cows. 高产奶牛代谢挑战与生产力的相互作用。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Geert Opsomer

{"title":"Interaction between metabolic challenges and productivity in high yielding dairy cows.","authors":"Geert Opsomer","doi":"","DOIUrl":"","url":null,"abstract":"The onset of lactation in modern dairy cows is characterized by a negative energy balance, due to a drastic increase in energy requirements for milk yield and a simultaneous depression in dry matter intake around parturition. Prioritization of milk yield over maternal body functions is a universal biological strategy in all lactating mammals to buffer the newborn's nutrition from fluctuations in the dam's energetic status. Consequently, in case of an energy deficiency, the dam will mobilize fat and protein reserves in order to safeguard milk yield. During decades of one-sided selection for milk yield, man has exploited the cow's potential to prioritize mammary energy supply without an equivalent progress in dry matter intake capacity. Consequently, genetic selection for milk yield has widened the gap between energy expenditure and energy intake, and has increased the cow's inclination to respond to energy deficiencies in the transition period by aggressive body tissue breakdown. Chronically elevated concentrations of non-esterified fatty acids and ketone bodies have been demonstrated to affect multiple organ systems including the immune system, the reproductive axis and the liver and are, in contrast to absolute milk yield, closely and consistently related to the final incidence of reproductive disorders.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological characteristics observed during early follicular development in perinatal MRL/MpJ mice. 围产期MRL/MpJ小鼠早期卵泡发育的形态学特征观察。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Yuma Yamashita, Teppei Nakamura, Saori Otsuka-Kanazawa, Osamu Ichii, Yasuhiro Kon

引用次数: 0

Factors contributing to immunosuppression in the dairy cow during the periparturient period. 奶牛围产期免疫抑制的影响因素。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Klaus L Ingvartsen, Kasey M Moyes

{"title":"Factors contributing to immunosuppression in the dairy cow during the periparturient period.","authors":"Klaus L Ingvartsen, Kasey M Moyes","doi":"","DOIUrl":"","url":null,"abstract":"The transition from late gestation to early lactation results in dramatic physiological changes including metabolic changes and immunosuppression in the dairy cow. As a result, cows are at a high risk for disease during this time. Evidence supporting a link between metabolic status and naturally occurring immunosuppression is growing. This review focuses on the impacts of metabolic status, and the metabolites that characterize it, on the immune response of cows during the transition period. Glucose is the preferred fuel for immune cells and its low concentration during the transition period may partly explain the naturally occurring immunosuppression at this time. To our knowledge, ketones are not utilized by immune cells and primarily have been shown to inhibit the immune response when concentration is relatively high. The effect of fatty acids on the immune system response remains unclear. Evidence suggests that the type of fatty acid can either stimulate (i.e. saturated fatty acids) or inhibit (i.e. unsaturated fatty acids) the immune response. We have suggested that an index for physiological imbalance (PI), based on circulating metabolites that characterize metabolic status, directly relates to mechanisms associated with the development of disease and is superior to calculated energy balance and therefore is a better predictor of risk of disease. The usefulness of the PI index as a predictor of risk of disease and the mechanisms associated with the links between degree of PI and immunosuppression for dairy cows during the transition period warrants further investigation.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subacute ruminal acidosis (SARA) challenge, ruminal condition and cellular immunity in cattle. 牛亚急性瘤胃酸中毒(SARA)挑战、瘤胃状况和细胞免疫。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Shigeru Sato

引用次数: 0

Genetic selection of cattle for improved immunity and health. 牛的遗传选择，以提高免疫力和健康。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-02-01

Bonnie A Mallard, Mehdi Emam, Marlene Paibomesai, Kathleen Thompson-Crispi, Lauraine Wagter-Lesperance

{"title":"Genetic selection of cattle for improved immunity and health.","authors":"Bonnie A Mallard, Mehdi Emam, Marlene Paibomesai, Kathleen Thompson-Crispi, Lauraine Wagter-Lesperance","doi":"","DOIUrl":"","url":null,"abstract":"The immune system is a sensing structure composed of tissues and molecules that are well integrated with the neuroendocrine system. This integrate system ensures non-self from self-discrimination. In this capacity the immune system provides detection and protection from a wide range of pathogens. In mammals, the immune system is regulated by several thousand genes (8-9% of the genome) which indicate its high genetic priority as a critical fitness trait providing survival of the species. Identifying and selectively breeding livestock with the inherent ability to make superior immune responses can reduce disease occurrence, improve milk quality and increase farm profitability. Healthier animals also may be expected to demonstrate improvements in other traits, including reproductive fitness. Using the University of Guelph's patented High Immune Response technology it is possible to classify animals as high, average, or low responders based on their genetic estimated breeding value for immune responsiveness. High responders have the inherent ability to produce more balanced and robust immune responses compared with average or low responders. High responders dairy cattle essentially have about one-half the disease occurrence of low responders, and can pass their superior immune response genes on to future generations thereby accumulating health benefits within the dairy herd.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0