Japanese Journal of Veterinary Research最新文献_第5页

Effectiveness of feline body mass index (fBMI) as new diagnostic tool for obesity. 猫体质量指数(fBMI)作为肥胖症新诊断工具的有效性。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Koh Kawasumi, Eiji Iwazaki, Yuki Okada, Toshiro Arai

引用次数: 0

Single nucleotide polymorphisms of Kit gene in Chinese indigenous horses. 中国本土马Kit基因的单核苷酸多态性。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Haoyuan Han, Chunchun Mao, Ningbo Chen, Xianyong Lan, Hong Chen, Chuzhao Lei, Ruihua Dang

{"title":"Single nucleotide polymorphisms of Kit gene in Chinese indigenous horses.","authors":"Haoyuan Han, Chunchun Mao, Ningbo Chen, Xianyong Lan, Hong Chen, Chuzhao Lei, Ruihua Dang","doi":"","DOIUrl":"","url":null,"abstract":"Kit gene is a genetic determinant of horse white coat color which has been a highly valued trait in horses for at least 2,000 years. Single nucleotide polymorphisms (SNPs) in Kit are of importance due to their strong associations with melanoblast survival during embryonic development. In this study, a mutation analysis of all 21 Kit exons in 14 Chinese domestic horse breeds revealed six SNPs (g.91214T>G, g.143245T>G, g.164297C>T, g.170189C>T, g.171356C>G, and g.171471G>A), which located in 5'-UTR region, intron 6, exon 15, exon 20, intron 20, and exon 21 of the equine Kit gene, respectively. Subsequently, these six SNPs loci were genotyped in 632 Chinese horses by PCR-RFLP or direct sequencing. The six SNPs together defined 18 haplotypes, demonstrating abundant haplotype diversities in Chinese horses. All the mutant alleles and haplotypes were shared among different breeds. But fewer mutations were detected in horses from China than that from abroad, indicating that Chinese horses belong to a more ancient genetic pool. This study will provide fundamental genetic information for evaluating the genetic diversity of Kit gene in Chinese indigenous horse breeds.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34680199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological investigation of Leptospira infection and its circulation in one intensive-type water buffalo farm in the Philippines. 菲律宾一个集约型水牛养殖场钩端螺旋体感染及其传播的血清学调查。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Marvin A Villanueva, Claro N Mingala, Nina G Gloriani, Yasutake Yanagihara, Norikazu Isoda, Chie Nakajima, Yasuhiko Suzuki, Nobuo Koizumi

{"title":"Serological investigation of Leptospira infection and its circulation in one intensive-type water buffalo farm in the Philippines.","authors":"Marvin A Villanueva, Claro N Mingala, Nina G Gloriani, Yasutake Yanagihara, Norikazu Isoda, Chie Nakajima, Yasuhiko Suzuki, Nobuo Koizumi","doi":"","DOIUrl":"","url":null,"abstract":"Water buffalo is an indispensable livestock in the Philippines. Leptospirosis is a serious zoonosis that can be fatal to humans and cause reproductive problems in livestock. Leptospirosis has been reported in some countries where water buffaloes are commercially raised, highlighting the Leptospira prevalence in this farming system, but information on leptospirosis in water buffalo farms in the Philippines is limited. In this study, we collected blood samples from rats (n = 21), and water buffaloes (n = 170) from different groups and locations in one intensive-type buffalo farm in the Philippines. Serum was analyzed by microscopic agglutination test (MAT). Anti-Leptospira antibodies reacting with serogroups Canicola, Icterohaemorrhagiae and Pomona were found in sera of 30% tested rats, and 48% of water buffalo sera tested positive for at least one Leptospira strain, in which serogroups Mini, Hebdomadis, Tarassovi and Pyrogenes were predominantly agglutinated. The number of seropositive young water buffaloes (< 1 year-old) was lower than that of older seropositive ones. Furthermore, sera from younger water buffaloes were reactive with single serotypes with low MAT titers, but older animals were reactive with multiple Leptospira strains with variable MAT titers. In addition, antibodies against serogroups Icterohaemorrhagiae and Pomona were detected in both animals. Finally, Leptospira infection was found associated with age and animal grouping, highlighting the impact of management in the persistence of leptospirosis at intensive-type buffalo farm settings in the Philippines. Further investigation and appropriate control strategies are required to prevent leptospirosis from causing risks to public health and economic losses to the water buffalo farming industry.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34517164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiologic study of dogs with the displacement or deformity of the medial and lateral fabellae in Japan. 日本犬内外侧腓骨移位或畸形的流行病学研究。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Shinji Yasukawa, Kazuya Edamura, Koji Tanegashima, Hirotaka Kai, Go Higuchi, Momoyo Nagasawa, Kenji Teshima, Kazushi Asano, Tomohiro Nakayama

{"title":"Epidemiologic study of dogs with the displacement or deformity of the medial and lateral fabellae in Japan.","authors":"Shinji Yasukawa, Kazuya Edamura, Koji Tanegashima, Hirotaka Kai, Go Higuchi, Momoyo Nagasawa, Kenji Teshima, Kazushi Asano, Tomohiro Nakayama","doi":"","DOIUrl":"","url":null,"abstract":"The prevalences of displacement and deformity of the medial and lateral fabellae in dogs were investigated. This was a retrospective epidemiologic study. Radiographs of canine stifle joints (1022 limbs, 534 dogs) were obtained. The images were taken at the Nihon University Animal Medical Center and three private animal hospitals from January 2003 to July 2012. The position and morphology of the medial or lateral fabella were evaluated on the radiographs. The prevalence of displacement of the medial and lateral fabellae was 1.7% and 0.3%, respectively. The prevalence of deformity of the medial and lateral fabellae was 6.9% and 4.6%, respectively. Aplasia or hypoplasia of the fabella was detected more frequently in the medial fabella. On the other hand, a bipartite or multipartite fabella was observed more frequently in the lateral fabella. Nearly all cases of displacement or deformity of the fabella occurred in dogs weighing less than 10 kg. Abnormalities of the fabella were observed in the medial and lateral fabella. We found that abnormal fabellae were closely associated with medial patellar luxation and to a lesser extent with cranial cruciate ligament rupture. No clinical signs were associated with an abnormality of the fabella, with the exception of two dogs with traumatic avulsion of the lateral head of the gastrocnemius muscle.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34517166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of one-step real-time reverse transcriptase-PCR-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea. 基于一步实时逆转录-聚合酶链反应的快速同时检测猪腹泻四种病毒的方法的建立。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Tsuneyuki Masuda, Shinobu Tsuchiaka, Tomoko Ashiba, Hiroshi Yamasato, Kazuhiro Fukunari, Tsutomu Omatsu, Tetsuya Furuya, Junsuke Shirai, Tetsuya Mizutani, Makoto Nagai

{"title":"Development of one-step real-time reverse transcriptase-PCR-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea.","authors":"Tsuneyuki Masuda, Shinobu Tsuchiaka, Tomoko Ashiba, Hiroshi Yamasato, Kazuhiro Fukunari, Tsutomu Omatsu, Tetsuya Furuya, Junsuke Shirai, Tetsuya Mizutani, Makoto Nagai","doi":"","DOIUrl":"","url":null,"abstract":"Porcine diarrhea caused by viruses is a major problem of the pig farming industry and can result in substantial losses of revenue. Thus, diagnosing the infectious agents is important to prevent and control diseases in pigs. We developed novel one-step real-time quantitative RT-PCR (qPCR) assays that can detect four porcine diarrheal viruses simultaneously: porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and porcine group A rotavirus (PRVA). The qPCR analysis takes only 75 minutes to detect the presence of the four viruses. The limits of detection of our new assays for PEDV, TGEV, PDCoV, and PRVA were 100, 10, 10 and 10 copies per reaction, respectively. The sensitivity of qPCR was 1-1000 times higher than that of published gel-based RT-PCR. We used our qPCR method to successfully diagnose clinical samples from infected pigs, and no false positive results were obtained. In conclusion, qPCR can drastically reduce the diagnostic time to detect viruses compared to currently employed methods. We predict that the qPCR assays will become a useful tool for detecting viral infections that cause diarrhea and other complications in pigs.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34517163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local variation of soil contamination with radioactive cesium at a farm in Fukushima. 福岛农场放射性铯污染土壤的局部变化。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Itaru Sato, Masahiro Natsuhori, Jun Sasaki, Hiroshi Satoh, Takahisa Murata, Tatsuro Nakamura, Kumiko Otani, Keiji Okada

引用次数: 0

Lambda cyhalothrin toxicity induces alterations in lipogenic genes and inflammatory factors in rat liver. 氯氟氰菊酯毒性诱导大鼠肝脏脂肪生成基因和炎症因子的改变。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-02-01

Gihan G Moustafa, Mohamed M A Hussein

{"title":"Lambda cyhalothrin toxicity induces alterations in lipogenic genes and inflammatory factors in rat liver.","authors":"Gihan G Moustafa, Mohamed M A Hussein","doi":"","DOIUrl":"","url":null,"abstract":"The present study aims to elucidate the molecular basis of lambda cyhalothrin (LCT) toxicity. Thirty-two mature male albino rats were randomly classified into four equal groups. The first group was orally administered normal saline, the second group was orally administered dimethylsulfoxide (DMSO). The third group was orally administered 1/100 LD50 (6.12 mg/kg b. wt) of a commercial formulation containing 2.5% LCT (i.e., a net dose LCT corresponding to 0.15 mg/kg b. wt). The fourth group was orally administered 1/100 LD50 (0.64 mg/kg b. wt) of a pure form of LCT. The results indicated that exposure to LCT is capable of inducing an up-regulation in the mRNA expression levels of peroxisome proliferative activated receptor α and γ (PPAR α and PPAR γ), tumor necrosis factor (TNF-α), fatty acid synthase (FAS) and sterol regulatory element binding protein-1c (SREBP-1c). Additionally, our study revealed a significant increase in serum levels of ALT, AST, ALP, γGT as well as the inflammatory cytokines TNF-α and monocyte chemoattractant protein-1 (MCP-1). A significant elevation in total lipids, total cholesterol, triacylglycerol, LDL-c and leptin with a corresponding significant decrease in HDL-c was also noted. Moreover, our results depicted that LCT treatment exhibits a significant increase in hepatic MDA levels concurrent with a significant decrease in GSH levels and the activities of CAT, SOD, and GPx. An immunohistochemical investigation also revealed a strong up-regulation of hepatic FAS in the LCT treated groups. The histopathological findings were marked by evidence in support of periportal fatty changes and interstitial aggregation of round cells.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34517165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of inhibitory materials against PEDV corona virus from medicinal plants 药用植物中PEDV冠状病毒抑制物质的发现

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2016-01-01 DOI: 10.14943/JJVR.64.SUPPL.S53

Jun Li Yang, T. Ha, W. Oh

引用次数: 17

Induction of immunocastration in pre-pubertal boars immunized with recombinant gonadotropin-releasing hormone conjugated with Salmonella Typhimurium flagellin fljB. 重组促性腺激素释放激素结合鼠伤寒沙门菌鞭毛蛋白fljB免疫诱导青春期前公猪免疫去势。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-05-01

Woo-Jung Park, Byung-Joo Park, Young-Jo Song, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Young-Gyu Jang, Hyoung-Moon Kim, Jang-Hyuck Han, Cheong-Hwan Jung, In-Soo Choi

{"title":"Induction of immunocastration in pre-pubertal boars immunized with recombinant gonadotropin-releasing hormone conjugated with Salmonella Typhimurium flagellin fljB.","authors":"Woo-Jung Park, Byung-Joo Park, Young-Jo Song, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Young-Gyu Jang, Hyoung-Moon Kim, Jang-Hyuck Han, Cheong-Hwan Jung, In-Soo Choi","doi":"","DOIUrl":"","url":null,"abstract":"Immunocastration is an alternative method used to replace surgical castration commonly performed in swine farms. In boars, the main effects of immunocastration are reduction of gonadotropin-releasing hormone (GnRH) and the resulting inhibition of testicular function. The aim of this study was to evaluate immunocastration efficacy in pre-pubertal boars vaccinated with a recombinant GnRH protein conjugated with Salmonella Typhimurium flagellin fljB (STF2). A total of 35 boars were assigned to three groups: the untreated group (n = 5), the surgically castrated group (n = 5), and the immunocastrated group (n = 25). Pigs in the immunocastration group were immunized with the GnRH-STF2 vaccine at pre-pubertal ages 4 and 8 weeks. All experimental pigs were kept for 26 weeks before slaughter. Anti-GnRH antibody levels of immunocastrated pigs were significantly higher than those of untreated pigs (P < 0.001). In contrast, testosterone levels of immunocastrated pigs were significantly lower than those of untreated pigs (P < 0.001). Statistical significances were not found in the body weights and backfat thicknesses of untreated vs. immunocastrated pigs. Weights of the testes and epididymides of immunocastrated pigs were significantly lower than those of untreated pigs (P < 0.001). Testicular tissues of immunocastrated pigs were severely suppressed compared with those of untreated pigs. In conclusion, immunization with the STF2-GnRH vaccine effectively induced immunocastration in pre-pubertal boars.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34280955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Schistosoma spindale ova and associated risk factors among Malaysian cattle through coprological survey. 通过粪学调查在马来西亚牛中检测纺锤形血吸虫卵及其相关危险因素。

IF 0.4 4区农林科学

Japanese Journal of Veterinary Research Pub Date : 2015-05-01

Tiong Kai Tan, Van Lun Low, Soo Ching Lee, Chandrawathani Panchadcharam, Sun Tee Tay, Romano Ngui, Premaalatha Bathmanaban, Kai Ling Kho, Fui Xian Koh, Reuben Sunil Kumar Sharma, Tariq Jaafar, Quaza Nizamuddin Hassan Nizam, Yvonne Ai Lian Lim

{"title":"Detection of Schistosoma spindale ova and associated risk factors among Malaysian cattle through coprological survey.","authors":"Tiong Kai Tan, Van Lun Low, Soo Ching Lee, Chandrawathani Panchadcharam, Sun Tee Tay, Romano Ngui, Premaalatha Bathmanaban, Kai Ling Kho, Fui Xian Koh, Reuben Sunil Kumar Sharma, Tariq Jaafar, Quaza Nizamuddin Hassan Nizam, Yvonne Ai Lian Lim","doi":"","DOIUrl":"","url":null,"abstract":"The present study was conducted to determine the occurrence of Schistosoma spindale ova and its associated risk factors in Malaysian cattle through a coprological survey. A total of 266 rectal fecal samples were collected from six farms in Peninsular Malaysia. The overall infection rate of S. spindale was 6% (16 of 266). Schistosoma spindale infection was observed in two farms, with a prevalence of 5.4% and 51.9%, respectively. This trematode was more likely to co-occur with other gastro-intestinal parasites (i.e., Dicrocoelium spp., Paramphistomum spp., strongyle, Eimeria spp. and Entamoeba spp.). Chi-square analysis revealed that female cattle are less likely to get S. spindale infection as compared to male cattle (OR = 0.3; 95% CI = 0.08-1.06; p < 0.05), and cattle weighing lower than 200 kg, were significantly at higher risk than those higher than 200 kg (OR = 5; 95% CI = 1.07-24.79; p < 0.05) to the infection. Multivariate analysis confirmed that among the cattle in Malaysia, the age (cattle with two year old and higher: OR = 21; 95% CI = 2.48-179.44; p < 0.05) and weight (weighing 200 kg and lower: OR = 17; 95% CI = 3.38-87.19; p < 0.05) were risk factors for S. spindale infection among Malaysian cattle.","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34280954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0