{"title":"A Multimodal Data Fusion and Embedding Attention Mechanism-Based Method for Eggplant Disease Detection.","authors":"Xinyue Wang, Fengyi Yan, Bo Li, Boda Yu, Xingyu Zhou, Xuechun Tang, Tongyue Jia, Chunli Lv","doi":"10.3390/plants14050786","DOIUrl":"10.3390/plants14050786","url":null,"abstract":"<p><p>A novel eggplant disease detection method based on multimodal data fusion and attention mechanisms is proposed in this study, aimed at improving both the accuracy and robustness of disease detection. The method integrates image and sensor data, optimizing the fusion of multimodal features through an embedded attention mechanism, which enhances the model's ability to focus on disease-related features. Experimental results demonstrate that the proposed method excels across various evaluation metrics, achieving a precision of 0.94, recall of 0.90, accuracy of 0.92, and mAP@75 of 0.91, indicating excellent classification accuracy and object localization capability. Further experiments, through ablation studies, evaluated the impact of different attention mechanisms and loss functions on model performance, all of which showed superior performance for the proposed approach. The multimodal data fusion combined with the embedded attention mechanism effectively enhances the accuracy and robustness of the eggplant disease detection model, making it highly suitable for complex disease identification tasks and demonstrating significant potential for widespread application.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050788
Dario Azzaro, Salvatore Cambria, Manuela Porrovecchio, Pietro Minissale
{"title":"Diachronic Analysis of the Floristic Diversity of the Special Area of Conservation (SAC) \"Bosco di Santo Pietro\" (South-Eastern Sicily): A Mediterranean Biodiversity Hotspot.","authors":"Dario Azzaro, Salvatore Cambria, Manuela Porrovecchio, Pietro Minissale","doi":"10.3390/plants14050788","DOIUrl":"10.3390/plants14050788","url":null,"abstract":"<p><p>This study presents a complete and updated checklist of the vascular flora of the SAC \"Bosco di Santo Pietro\", an important natural area located in south-eastern Sicily. Through an integrated approach combining field research, analysis of historical herbariums and a literature review, 583 taxa belonging to 78 families and 339 genera were identified. A comparison with an older floristic list of the Santo Pietro Forest dating back to 1889 highlights some significant changes, such as a decrease in several hygrophilous and nemoral species probably due to climate change and habitat degradation. In particular, our diachronic analysis reveals the disappearance of 178 taxa and the persistence of 199 taxa representing 47% and 53% of the 377 taxa listed in the first inventory, respectively. From the study of the Ellenberg indicator of the two lists, lower values of L and T and higher values of M and N emerge in the older flora, testifying to the significant environmental modifications for more than a century of this Sicilian and Mediterranean hotspot.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050783
Trajče Stafilov, Katerina Bačeva Andonovska, Robert Šajn, Marija Jeftimova
{"title":"Assessing the Distribution of Potentially Toxic Elements in Bryophytes in Relation to Surface Soil Contamination in the Veles Region, North Macedonia.","authors":"Trajče Stafilov, Katerina Bačeva Andonovska, Robert Šajn, Marija Jeftimova","doi":"10.3390/plants14050783","DOIUrl":"10.3390/plants14050783","url":null,"abstract":"<p><p>This study explores the relationship between bryophyte (mosses) diversity and environmental factors in the Veles region, North Macedonia, focusing on the spatial distribution of chemical elements in the moss and surface soil samples collected from the same locations. Eighteen moss samples were analyzed alongside surface soils. Advanced spectrometric techniques were used to identify potentially toxic elements (PTEs) and their links to anthropogenic and natural sources. While metal measurements are widely reported in the literature, the novelty of this study lies in its integrative approach, combining moss biodiversity analysis with a direct comparison of element concentrations in both moss and soil. The results show significant patterns of deposition of PTEs and highlight the long-term impact of industrial activities on biodiversity and air pollution. These findings provide valuable insights into conservation strategies and environmental management in the midst of ongoing ecological change. Five groups of elements were separated using factor analysis: G1 (Al, Cr, Cu, Fe, Li, Mg, Mn, Ni and V); G2 (Ba and Na); G3 (K, P and Mo), G4 (Pb and Zn), and G5 (Ag, As and Cd), of which two groups (G1 and G2) were found to be typical geochemical associations, while G4 and G5 are anthropogenic associations due to the emission of dust from contaminated soils and the slag heap of the Pb-Zn smelting plant. Group 3 represents a mixed geochemical and anthropogenic association. It was found that Pb, Zn, Cd, and As could indeed be detected in the moss in the study area, underlining its ability to detect pollutants in the air. A comparative analysis of moss and soil samples revealed significant differences in element concentrations, with most elements being more concentrated in soil. These results underline the role of moss as a bioindicator of atmospheric deposition, detecting pollution trends rather than direct soil contamination.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050791
Lei Han, Lerong Ge, Lin Fei, Chengwei Huang, Yilin Li, Wentan Fan, Dan Zhu, Longgang Zhao
{"title":"A Comprehensive Evaluation of Soybean Germplasm Resources for Salt Tolerance During Germination.","authors":"Lei Han, Lerong Ge, Lin Fei, Chengwei Huang, Yilin Li, Wentan Fan, Dan Zhu, Longgang Zhao","doi":"10.3390/plants14050791","DOIUrl":"10.3390/plants14050791","url":null,"abstract":"<p><p>Salt stress impedes normal development, compromises plant quality, and reduces crop yield. The germination phase in soybean marks the initial stage of its growth cycle. Characterizing salt tolerance during this period can help stimulate soybean growth in natural environments and aid the rapid screening of salt-tolerant soybean varieties. Our study characterized the salt tolerance of 36 soybean germplasms in culture dishes during the germination period. Soybeans were subjected to varying concentrations (0, 60, 120, and 180 mmol/L) of NaCl solution to simulate diverse levels of salt stress, and parameters such as germination energy, germination rate, and root length were measured. Statistical techniques such as analysis of variance, membership function, cluster analysis, and quadratic regression equations were used, and the salt tolerance of these 36 soybean germplasms was determined. The critical indicators and the most effective screening concentration for assessing the germination salt tolerance of soybean were identified. Soybeans tolerated low salt concentrations; however, salt concentrations greater than 120 mmol/L significantly inhibited germination indicators. The germination rate, germination vigor, vitality index, seed germination index, total fresh weight, and total dry weight could be used to identify salt tolerance. The semi-lethal concentration of soybean was 155.4 mmol/L, and the coefficient of variation was 20.00%, indicating that it could be used as a screening concentration for evaluating salt tolerance during soybean germination. A total of 36 soybean varieties were classified into four salt tolerance levels through cluster analysis. QN-27, QN-35, and QN-36 were highly salt-resistant materials, and QN-2, QN-17, and QN-19 were salt-sensitive materials. Characterizing salt tolerance during soybean germination can facilitate the selection and breeding of salt-tolerant soybean varieties. Future research utilizing this approach can aid in the selection of soybean varieties with salinity tolerance.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Clay Amendment and Strategic Deep Tillage on Soil Water Dynamics and Plant Growth Under Controlled Environments.","authors":"Kanchana Wickramarachchi, Giacomo Betti, Gaus Azam","doi":"10.3390/plants14050799","DOIUrl":"10.3390/plants14050799","url":null,"abstract":"<p><p>Strategic deep tillage (SDT) practices, such as soil mixing following the application of soil amendments, are promising approaches to alleviate topsoil water repellence and other subsoil constraints and improve crop productivity. However, there is a lack of knowledge on the effect of SDT on soil water dynamics, especially under water-limited environments. This study evaluates the effects of clay incorporation, soil inversion and deep soil mixing on soil water infiltration, surface evaporation rates, soil water storage and subsequent impacts on the below and aboveground growth of wheat (<i>Triticum aestivum</i> L. var Scepter) in controlled environments. Results show that soil mixing significantly improved water infiltration compared to an untreated control. Clay incorporation exhibited the highest bare soil surface evaporation rates immediately and two years post-tillage, leading to substantial water losses under warm and dry ambient conditions. Despite improving soil water storage in deeper layers, high evaporation rates in clay-incorporated soils negatively impacted wheat growth, with reduced shoot biomass and root length density. Conversely, soil inversion and mixing-only treatments demonstrated balanced improvements in water infiltration, soil water use, and wheat shoot biomass. These findings underscore the trade-offs associated with SDT practices, particularly in managing soil water loss and crop productivity in water-limited environments. This study also highlights the need for the careful selection of SDT for soil amelioration strategies tailored to soil types and climatic conditions to enhance agricultural productivity and sustainability.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050795
Emilio Di Gristina, Giulio Barone, Gianniantonio Domina, Emilio Badalamenti, Maria Letizia Gargano, Giuseppe Venturella, Raimondo Pardi
{"title":"A Checklist of the Ornamental Vascular Flora of Sicily.","authors":"Emilio Di Gristina, Giulio Barone, Gianniantonio Domina, Emilio Badalamenti, Maria Letizia Gargano, Giuseppe Venturella, Raimondo Pardi","doi":"10.3390/plants14050795","DOIUrl":"10.3390/plants14050795","url":null,"abstract":"<p><p>Based on literature data and new field investigations, a checklist of the ornamental vascular flora of Sicily is presented. Trees, shrubs, herbaceous, and succulent plants growing in street trees, parks, private gardens, and historic villas of Sicily are included in the checklist. For each taxon, information on growth forms, geographical and biome origin, resident time, and status (native/alien) in Italy is provided. A total of 928 taxa (including 823 species s. str., 33 cultivars, 31 hybrids, 24 varieties, 9 subspecies, and 8 forms), belonging to 486 genera included in 138 families, were recorded. Fabaceae, Rosaceae, Asteraceae, Cactaceae, Asparagaceae, Arecaceae, and Malvaceae are the richest families in taxa. Phanerophytes are the prevalent growth form, and the main part of such flora consists of Asiatic and American taxa. Taxa from subtropical and tropical biomes showed a prevalent presence; this is a peculiarity which characterizes the Sicilian ornamental flora. <i>Delonix regia</i> emerges as a peculiar case, representing the only documented cases of open-air cultivation in both Sicily and the entire country. A significant percentage of taxa (41%) is included in the list of alien vascular flora of Italy, showing a strong predominance of casual and naturalized neophytes alien species.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050789
Eduardo Estrada Castillón, José Ángel Villarreal Quintanilla, Juan Antonio Encina Domínguez, Arturo Mora Olivo, Jaime Sánchez Salas, Gisela Muro Pérez, Eduardo Alanís Rodríguez, Renata Aidé Valdés Alameda, Nelly Sandoval Mata, Gilberto Ocampo
{"title":"The Fabaceae in Northeastern Mexico (Subfamily Papilionoideae, Tribes Amorpheae, Brongniartieae, and Dalbergieae).","authors":"Eduardo Estrada Castillón, José Ángel Villarreal Quintanilla, Juan Antonio Encina Domínguez, Arturo Mora Olivo, Jaime Sánchez Salas, Gisela Muro Pérez, Eduardo Alanís Rodríguez, Renata Aidé Valdés Alameda, Nelly Sandoval Mata, Gilberto Ocampo","doi":"10.3390/plants14050789","DOIUrl":"10.3390/plants14050789","url":null,"abstract":"<p><p>A compendium of the legumes of the subfamily Papilionoideae, tribes Amorpheae, Brongniarteae, and Dalbergieae in northeastern Mexico is presented for the first time, including changes in their botanical nomenclature within tribes and genera. Based on recently published studies, the taxonomic limits of several genera and new ones segregated such as <i>Marina</i> and <i>Ctenodon</i> are clarified and included. Based mainly on fieldwork over the past 40 years, as well as reviewing specimens in national and international herbaria, we show the total diversity of legumes of the subfamily Papilionoideae, tribes Amorpheae, Brongniarteae, and Dalbergieae. The three tribes include 16 genera and 75 species. Tribe Amorpheae comprises five genera (<i>Amorpha</i>, <i>Dalea</i>, <i>Eysenhardtia</i>, <i>Marina</i>, and <i>Psorothamnus</i>) and forty-three species; tribe Brongniartieae comprises two genera <i>Brongniartia</i> and <i>Harpalyce</i>) and eight species; and tribe Dalbergieae comprises nine genera (<i>Aeschynomene</i>, <i>Amicia</i>, <i>Arachis</i>, <i>Ctneodon</i>, <i>Dalbergia</i>, <i>Diphysa</i>, <i>Nissolia</i>, <i>Stylosanthes</i>, and <i>Zornia</i>) and twenty-four species. <i>Dalea</i> is by far the genus with the highest number of species and infraspecific categories, as well as in a number of endemisms because 17 (51%) of them are endemic to Mexico, and six of them are endemic to the northeastern part of the country. Of the 13 species of <i>Eysenhardtia</i> present in Mexico, 31% of them reach the northeast region and three of them are exclusive to this region. There are no species of the Brongniartieae and Dalbergieae tribes endemic to northeastern Mexico, but 10 of their species are endemic to Mexico.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050798
Arne Langhoff, Astrid Peschel, Christian Leppin, Sebastian Kruppert, Thomas Speck, Diethelm Johannsmann
{"title":"Rapid Solidification of Plant Latices from <i>Campanula glomerata</i> Driven by a Sudden Decrease in Hydrostatic Pressure.","authors":"Arne Langhoff, Astrid Peschel, Christian Leppin, Sebastian Kruppert, Thomas Speck, Diethelm Johannsmann","doi":"10.3390/plants14050798","DOIUrl":"10.3390/plants14050798","url":null,"abstract":"<p><p>By monitoring the solidification of droplets of plant latices with a fast quartz crystal microbalance with dissipation monitoring (QCM-D), droplets from <i>Campanula glomerata</i> were found to solidify much faster than droplets from <i>Euphorbia characias</i> and also faster than droplets from all technical latices tested. A similar conclusion was drawn from optical videos, where the plants were injured and the milky fluid was stretched (sometimes forming fibers) after the cut. Rapid solidification cannot be explained with physical drying because physical drying is transport-limited and therefore is inherently slow. It can, however, be explained with coagulation being triggered by a sudden decrease in hydrostatic pressure. A mechanism based on a pressure drop is corroborated by optical videos of both plants being injured under water. While the liquid exuded by <i>E. characias</i> keeps streaming away, the liquid exuded by <i>C. glomerata</i> quickly forms a plug even under water. Presumably, the pressure drop causes an influx of serum into the laticifers. The serum, in turn, triggers a transition from a liquid-liquid phase separated state (an LLPS state) of a resin and hardener to a single-phase state. QCM measurements, optical videos, and cryo-SEM images suggest that LLPS plays a role in the solidification of <i>C. glomerata</i>.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050790
Tianwen Wang, Xianxiang Luo, Kun Chen, Hao Zheng, Zewei Song, Lize Zhao, Junhua Gong, Fengmin Li, Ruhai Liu
{"title":"Effects of Mg-Palygorskite Modified Biochar on the Growth of <i>Sedum alfredii</i> Hance in Heavy Metal Contaminated Soil.","authors":"Tianwen Wang, Xianxiang Luo, Kun Chen, Hao Zheng, Zewei Song, Lize Zhao, Junhua Gong, Fengmin Li, Ruhai Liu","doi":"10.3390/plants14050790","DOIUrl":"10.3390/plants14050790","url":null,"abstract":"<p><p>Heavy metal contamination of soil poses a serious threat to agricultural production and human health. Biochar modified with Mg and palygorskite can reduce the content of available heavy metals in soil; however, its passivation effect is affected by the modification method, and there is a lack of research on its impact on plant growth in heavily polluted soil. In this study, four types of modified biochar were prepared using MgCl<sub>2</sub>, palygorskite, and wood as raw materials, including MBC and MPB prepared by pre-modification and BCM and BPM prepared by post-modification. <i>Sedum alfredii</i> Hance was selected as the test plant, and a pot experiment was conducted to explore the effects of unmodified and modified biochar on the growth of <i>Sedum alfredii</i> Hance in heavily polluted soil with Cu, Pb, Zn, and Cd. Compared with the original biochar, the modified biochar, especially the pre-modified biochar, significantly increased the ash content, pH, O/C ratio, surface functional group count, and mineral content. The adsorption capacity for heavy metals was also significantly enhanced, with the main adsorption mechanisms being precipitation, complexation, and ion exchange. The four types of modified biochar promoted the growth and biomass of <i>Sedum alfredii</i> Hance to varying degrees, with the promotion effect in the order of MPB > MBC > BPM > BCM, and the effect was more significant with a 3% addition. The modified biochar significantly reduced the content of available heavy metals in the rhizosphere soil, with a passivation effect in the order of MPB > MBC > BPM > BCM, and the 3% addition had the greatest effect. Further analysis via the Mantel test and structural equation modeling confirmed that modified biochar promoted the growth of <i>Sedum alfredii</i> Hance by reducing the available heavy metal content in the rhizosphere soil and increasing the NO<sub>3</sub><sup>-</sup>-N and AP contents. This study provides data support for the development of functionalized biochar for the remediation of heavy metal pollution in soil.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants-BaselPub Date : 2025-03-04DOI: 10.3390/plants14050782
Yafei Tang, Mengdan Du, Zhenggang Li, Lin Yu, Guobing Lan, Shanwen Ding, Tahir Farooq, Zifu He, Xiaoman She
{"title":"Identification and Genome Characterization of Begomovirus and Satellite Molecules Associated with Lettuce (<i>Lactuca sativa</i> L.) Leaf Curl Disease.","authors":"Yafei Tang, Mengdan Du, Zhenggang Li, Lin Yu, Guobing Lan, Shanwen Ding, Tahir Farooq, Zifu He, Xiaoman She","doi":"10.3390/plants14050782","DOIUrl":"10.3390/plants14050782","url":null,"abstract":"<p><p>Lettuce (<i>Lactuca sativa</i> L.) plants showing leaf curl and vein enation symptoms were found in Yunnan province, China. PCR detection with genus-specific primers revealed that symptomatic lettuce plants were infected with <i>Begomovirus</i>. The full-length viral component and satellite molecules were obtained by RCA, restriction enzyme digestion, PCR, cloning and DNA sequencing. A viral component (YN-2023-WJ) and three satellite molecules (YN-2023-WJ-alpha1, YN-2023-WJ-alpha2 and YN-2023-WJ-beta) were obtained from diseased lettuce plants. YN-2023-WJ exhibited the highest nt identity at 97.1% with pepper leaf curl Yunnan virus isolated from cigar plants. YN-2023-WJ-beta displayed the highest nt identity at 93.9% with tomato leaf curl China betasatellite. YN-2023-WJ-alpha1 showed the highest nt identity at 94.7% with ageratum yellow vein alphasatellite. YN-2023-WJ-alpha2 shared the highest nt identity at 75.6% with gossypium mustelinum symptomless alphasatellite and vernonia yellow vein Fujian alphasatellite. Based on the threshold for the classification of <i>Begomovirus</i>, <i>Betasatellite</i> and <i>Alphasatellite</i>, YN-2023-WJ was designated as a new isolate of PepLCYnV, YN-2023-WJ-beta as a new isolate of ToLCCNB and YN-2023-WJ-alpha1 as a new member of AYVA, whereas YN-2023-WJ-alpha2 was identified as a new geminialphasatellite species, for which the name pepper leaf curl Yunnan alphasatellite (PepLCYnA) is proposed. To the best of our knowledge, this is the first report of <i>L. sativa</i> L. infection by PepLCYnV associated with ToLCCNB, AYVA and PepLCYnA, and <i>L. sativa</i> L. is a new host plant of <i>Begomovirus</i>.</p>","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}