Rice Science最新文献

{"title":"Insights into Anticancer Activity of Indian Aromatic Rice Callus Suspension Culture on Colon and Lung Cancer Cell Lines by Proteomic Analysis","authors":"Anuradha Kumari, Wusirika Ramakrishna","doi":"10.1016/j.rsci.2025.04.002","DOIUrl":"10.1016/j.rsci.2025.04.002","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 303-306"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Folate Content in Japonica Rice Through Co-expression of OsADCS and OsGTPCHI Indica Alleles","authors":"Lai Changkai ,&nbsp;Hu Shikai ,&nbsp;Jiao Guiai ,&nbsp;Wang Ling ,&nbsp;Shao Gaoneng ,&nbsp;Zhao Fengli ,&nbsp;Xie Lihong ,&nbsp;Wei Xiangjin ,&nbsp;Lü Yusong ,&nbsp;Sheng Zhonghua ,&nbsp;Tang Shaoqing ,&nbsp;Hu Peisong","doi":"10.1016/j.rsci.2025.01.008","DOIUrl":"10.1016/j.rsci.2025.01.008","url":null,"abstract":"<div><div>Rice is a poor source of folate, an essential micronutrient for the body. Biofortification offers an effective way to enhance the folate content of rice and alleviate folate deficiencies in humans. In this study, we confirmed that <em>OsADCS</em> and <em>OsGTPCHI</em>, encoding the initial enzymes necessary for folate synthesis, positively regulate folate accumulation in knockout mutants of both <em>japonica</em> and <em>indica</em> rice backgrounds. The folate content in the low-folate <em>japonica</em> variety was slightly increased by the expression of the <em>indica</em> alleles driven by the endosperm-specific promoter. We further obtained co-expression lines by stacking <em>OsADCS</em> and <em>OsGTPCHI</em> genes; the folate accumulation in brown rice and polished rice reached 5.65 μg/g and 2.95 μg/g, respectively, representing 37.9-fold and 26.5-fold increases compared with the wild type. Transcriptomic analysis of rice grains from six transgenic lines showed that folate changes affected biological pathways involved in the synthesis and metabolism of rice seed storage substances, while the expression of other folate synthesis genes was weakly regulated. In addition, we identified <em>Aus</em> rice as a high-folate germplasm carrying superior haplotypes of <em>OsADCS</em> and <em>OsGTPCHI</em> through natural variation. This study provides an alternative and effective complementary strategy for rice biofortification, promoting the rational combination of metabolic engineering and conventional breeding to breed high-folate varieties.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 353-366"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Advanced Genomic Approaches to Unveil and Enhance Brown Planthopper Resistance in Rice","authors":"Lakshmi V.G. Ishwarya ,&nbsp;S. Vanisri ,&nbsp;P.S. Basavaraj ,&nbsp;M. Sreedhar ,&nbsp;Lakshmi V. Jhansi ,&nbsp;M. Muntazir ,&nbsp;C. Gireesh ,&nbsp;S.N.C.V.L. Pushpavalli","doi":"10.1016/j.rsci.2025.04.003","DOIUrl":"10.1016/j.rsci.2025.04.003","url":null,"abstract":"<div><div>Brown planthopper (BPH) is a highly destructive pest that presents a significant challenge to rice production, particularly in the Asia-Pacific region. Numerous BPH-resistant rice varieties have been successfully bred and released for commercial cultivation across diverse rice-growing ecosystems. However, resistance breakdown in several varieties carrying major resistance genes has been reported, highlighting the urgent need for the development of novel, genetically diverse, and broad-spectrum resistant varieties. To date, more than 45 resistance loci have been identified and mapped from both cultivated and wild rice species. Among these, a subset of genes (including <em>Bph1</em>, <em>Bph3</em>, <em>Bph6</em>, <em>Bph7</em>, <em>Bph9</em>, <em>Bph10</em>, <em>Bph14</em>, <em>Bph15</em>, <em>Bph18</em>, <em>Bph21</em>, <em>Bph26/2</em>, <em>bph29</em>, <em>Bph32</em>, <em>Bph37</em>, and <em>Bph30/Bph40</em>) have been positionally cloned. Most of these genes encode coiled-coil nucleotide-binding leucine-rich repeat proteins, which are central to plant immune responses, along with a few signaling molecules playing pivotal roles. In addition to these core resistance genes, various other genetic components, including miRNAs, protein kinases, and transcription factors, have been functionally characterized for their roles in mediating BPH resistance. The advent of post-genomic tools such as RNA sequencing and single-cell sequencing, along with cutting-edge genomic technologies like CRISPR/Cas gene editing, has significantly accelerated resistance breeding programs. In this context, we provide an overview of genetics, mapping, isolation, and functional characterization of BPH resistance, along with strategies for incorporating resistance using advanced genomics-assisted breeding tools. Furthermore, we present a snapshot of how the integration of genomics and novel breeding technologies holds great promise for dissecting the genetic architecture of pest resistance and accelerating crop improvement.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 339-352"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsERF7 Negatively Regulates Resistance to Sheath Blight Disease by Inhibiting Phytoalexin Biosynthesis","authors":"Xie Yuhao ,&nbsp;Xie Wenya ,&nbsp;Zhao Jianhua ,&nbsp;Xue Xiang ,&nbsp;Cao Wenlei ,&nbsp;Shi Xiaopin ,&nbsp;Wang Zhou ,&nbsp;Wang Yiwen ,&nbsp;Wang Guangda ,&nbsp;Feng Zhiming ,&nbsp;Hu Keming ,&nbsp;Chen Xijun ,&nbsp;Chen Zongxiang ,&nbsp;Zuo Shimin","doi":"10.1016/j.rsci.2024.12.014","DOIUrl":"10.1016/j.rsci.2024.12.014","url":null,"abstract":"<div><div>Ethylene response factors (ERFs) are plant transcription agents that play a pivotal role in disease resistance through the ethylene signaling pathway. However, whether and how ERFs regulate resistance to sheath blight (ShB), caused by <em>Rhizoctonia solani</em> in rice, remains largely unknown. Here, we demonstrated that <em>OsERF7</em> negatively regulates rice resistance to ShB by inhibiting phytoalexin biosynthesis. Overexpression of <em>OsERF7</em> (<em>OsERF7OE</em>) significantly decreased ShB resistance, whereas knockout of <em>OsERF7</em> (<em>oserf7</em>) enhanced it. Mechanistically, antioxidant enzyme activities are significantly reduced in <em>OsERF7OE</em> plants, but increased in <em>oserf7</em> plants. Furthermore, transcriptome analysis revealed that <em>oserf7</em> plants exhibited significant upregulation of pathogenesis-related (PR) and phytoalexin biosynthesis genes upon <em>R. solani</em> infection. Consistently, transcript levels of phytoalexin biosynthesis genes, including <em>OsKSL7</em>, <em>OsKSL8</em>, <em>OsKOL5</em>, and <em>OsCPS4</em>, were significantly elevated in <em>oserf7</em> plants, but reduced in <em>OsERF7OE</em> plants in response to <em>R. solani</em> infection. Electrophoretic mobility shift assays and dual-luciferase (LUC) reporter assays further confirmed that <em>OsERF7</em> directly binds to the promoters of <em>OsKSL8</em>, <em>OsKOL5</em>, and <em>OsCPS4</em>, thereby repressing their expression. In summary, our study revealed that <em>OsERF7</em> negatively regulated rice resistance to ShB primarily by inhibiting phytoalexin biosynthesis.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 367-379"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning of qHD8BAS, a Quantitative Trait Locus Regulating Heading Date in Rice","authors":"Zhengjiu Zhang ,&nbsp;Ying Bian ,&nbsp;Ruoju Yang ,&nbsp;Xiaobo Zhang ,&nbsp;Junyi Gong ,&nbsp;Jiongjiong Fan","doi":"10.1016/j.rsci.2024.12.009","DOIUrl":"10.1016/j.rsci.2024.12.009","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 283-286"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Changes in Root Architecture, Developmental Timing, Transcriptional and Hormonal Profiles in Rice Co-Cultivated with Azolla filiculoides","authors":"Sara Cannavò ,&nbsp;Chiara Paleni ,&nbsp;Alma Costarelli ,&nbsp;Maria Cristina Valeri ,&nbsp;Martina Cerri ,&nbsp;Antonietta Saccomanno ,&nbsp;Veronica Gregis ,&nbsp;Graziella Chini Zittelli ,&nbsp;Petre I. Dobrev ,&nbsp;Lara Reale ,&nbsp;Martin M. Kater ,&nbsp;Francesco Paolocci","doi":"10.1016/j.rsci.2025.03.004","DOIUrl":"10.1016/j.rsci.2025.03.004","url":null,"abstract":"<div><div>Strategies for increasing rice yield are needed to keep pace with the expected global population growth and sustainably address the challenges posed by climate change. In Southeast Asian countries, rice farming benefits from the use of <em>Azolla</em> spp. for nitrogen supply. By virtue of their symbiosis with the nitrogen-fixing cyanobacterium <em>Trichormus azollae</em>, <em>Azolla</em> spp. are ferns that release nitrogen into the environment upon biomass decomposition. However, whether and to what extent actively growing <em>Azolla</em> plants influence the development of co-cultivated rice seedlings remains unclear. To address this, rice (<em>Oryza sativa</em> L. var. Kitaake) seedlings were co-cultivated hydroponically with <em>Azolla filiculoides</em> for up to two months. Morphological changes in rice roots and aerial organs were assessed alongside nitric oxide assays in rice roots, root transcriptomics, and targeted hormonomics of rice roots, leaves, and growth media. Here, we showed that co-cultivation with actively growing <em>A. filiculoides</em> alters rice root architecture by inducing a nitric oxide boost and accelerates leaf and tiller differentiation and proliferation. Overall, this study provides an in-depth analysis of the morphogenetic effects of co-cultivated <em>A. filiculoides</em> on rice during early vegetative growth. It also paves the way for studies assessing whether <em>A. filiculoides</em> co-cultivation primes rice plants to better withstand abiotic and biotic stresses.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 426-444"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chak-hao, Forbidden Rice of Manipur and Its Sustainable Protection from Post-Harvest Storage Pests Using Indigenous Botanical Plant Powders","authors":"Arati Ningombam ,&nbsp;Aruna Beemrote ,&nbsp;Romila Akoijam ,&nbsp;Sushmita Thokchom ,&nbsp;C.H. Basudha ,&nbsp;C.H. Sonia ,&nbsp;C.H. Premabati ,&nbsp;N. Ajitkumar Singh ,&nbsp;L. Langlentombi Chanu ,&nbsp;Y. Prabhabati Devi ,&nbsp;H. Lembisana Devi ,&nbsp;A. Gangarani Devi","doi":"10.1016/j.rsci.2025.04.001","DOIUrl":"10.1016/j.rsci.2025.04.001","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 298-302"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo Haploid Induction via Parthenogenesis Gene ToPAR in Rice","authors":"Jie Xiong ,&nbsp;Yajie Ji ,&nbsp;Shenlin Yang ,&nbsp;Xianjin Qiu ,&nbsp;Qian Qian ,&nbsp;Kejian Wang","doi":"10.1016/j.rsci.2024.12.006","DOIUrl":"10.1016/j.rsci.2024.12.006","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 273-276"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Management Strategies for Rice Leaffolder, Cnaphalocrocis medinalis (Guenée): Progress and Prospects","authors":"Yajun Yang,&nbsp;Yanhui Lu,&nbsp;Junce Tian,&nbsp;Xusong Zheng,&nbsp;Jiawen Guo,&nbsp;Xiaowei Liu,&nbsp;Zhongxian Lü,&nbsp;Hongxing Xu","doi":"10.1016/j.rsci.2024.12.011","DOIUrl":"10.1016/j.rsci.2024.12.011","url":null,"abstract":"<div><div>The rice leaffolder, <em>Cnaphalocrocis medinalis</em> (Guenée), is a major migratory insect pest in paddy fields that damages rice by folding and feeding on leaves, causing chlorophyll loss and resulting in significant yield losses when its population density exceeds an economic threshold. Sustainable pest management requires ‘green plant protection’ solutions. Advances in science and technology have introduced numerous green methods for sustainable management of the rice leaffolder. This paper reviews recent research advancements in rice leaffolder management, such as ecological regulation, healthy cultivation, behavioral regulation, biological control, and rational insecticide application. Based on accurate monitoring and early warning systems, rice leaffolder management can incorporate comprehensive green control products, green control technologies, and control modes. This paper provides prospects for discussing the future of rice leaffolder management, achieving sustainable management of the rice leaffolder, and ensuring rice production safety.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 322-338"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LR is a Novel Gene Regulating Amylose Content in Rice Revealed by Genome-Wide Association Study","authors":"Huijuan Li ,&nbsp;Haipeng Yu ,&nbsp;Guanrong Huang ,&nbsp;Zengying Huang ,&nbsp;Lu Tang ,&nbsp;Pengfei Yang ,&nbsp;Zhengzheng Zhong ,&nbsp;Guocheng Hu ,&nbsp;Peng Zhang ,&nbsp;Hanhua Tong","doi":"10.1016/j.rsci.2024.12.010","DOIUrl":"10.1016/j.rsci.2024.12.010","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 3","pages":"Pages 277-282"},"PeriodicalIF":5.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}