Rice Science最新文献

Submergence-Tolerant Rice Cultivar FR13A Harbors Floating Genes SNORKELs but Lacks Floating Ability

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.12.013

Daisuke Sasayama, Natsuki Hayashi, Shunsuke Oe, Hiroshi Fukayama, Tomoko Hatanaka, Tetsushi Azuma

引用次数: 0

A Meta-Analysis of 30 Years in China and Micro-District Experiments Shows Organic Fertilizer Quantification Combined with Chemical Fertilizer Reduction Enhances Rice Yield on Saline-Alkali Land

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2025.01.004

He Chen , Ruan Yunze , Jia Zhongjun

{"title":"A Meta-Analysis of 30 Years in China and Micro-District Experiments Shows Organic Fertilizer Quantification Combined with Chemical Fertilizer Reduction Enhances Rice Yield on Saline-Alkali Land","authors":"He Chen , Ruan Yunze , Jia Zhongjun","doi":"10.1016/j.rsci.2025.01.004","DOIUrl":"10.1016/j.rsci.2025.01.004","url":null,"abstract":"<div><div>To improve the yield and quality of rice grown on saline-alkali soil, a meta-analysis combined with micro-district experimental studies was conducted in China to examine the impact of humic acid- based organic fertilizer and chemical fertilizer on rice yield and quality. This study employed a two-factor fully randomized experimental design, incorporating four levels of humic acid (F0, 0.0 g/pot; F1, 4.8 g/pot; F2, 12.0 g/pot; and F3, 19.2 g/pot) and three levels of chemical fertilizer (A1, full conventional dosage; A2, 85% of conventional dosage; and A3, 70% of conventional dosage). The meta-analysis revealed that the application of organic fertilizer (at a rate of 1500‒3000 kg/hm<sup>2</sup>) combined with chemical fertilizer had a significantly positive effect on the theoretical yield, tiller number, partial factor productivity, and SPAD value of rice. Temperature, organic fertilizer application, and chemical fertilizer levels were identified as critical factors affecting rice yield. The micro-experiments demonstrated that the application of humic acid organic fertilizer with treatment F3 significantly elevated the SPAD value at the full heading and grain filling stages. Increased panicle number and seed-setting rate were the main contributors to the rise in yield, with the F3 treatment yielding the highest overall. The effective leaf area, high-efficiency leaf area, and dry matter accumulation in rice treated with F3 were all higher compared with the F0 treatment. Our findings indicated that the addition of humic acid organic fertilizer can markedly improve the partial factor productivity and agronomic efficiency of rice. In conclusion, the application of F3 organic fertilizer combined with A3 chemical fertilizer (F3A3) significantly increased the yield of saline-alkali rice, which was 6.62% higher than that of the F0A1 treatment, thereby validating the meta-analysis outcomes. We propose that the combined use of humic acid organic fertilizer and chemical fertilizer can promote the growth of rice in saline-alkali soils. Consequently, these management practices provide a means to foster the green and healthy development of rice in saline-alkali regions across China.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 259-272"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping of Red Hull Gene OsRH3 and Its Great Value in Mechanized Hybrid Rice Seed Production

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.12.005

Fang Pengpeng , Sun Pingyong , Zhang Li , Wang Pengfei , Zeng Jia , He Qiang , Shu Fu , Xing Yongzhong , Deng Huafeng , Zhang Wuhan

引用次数: 0

NARROW AND ROLLED LEAF 9, a Novel Gene Encoding 20S Proteasome β1 Subunit that Regulates Leaf Morphology and Grain Size in Rice

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.12.008

Fengyu Kang , Liang Zhou , Wenqiang Chen , Pingliang Huang, Amos Musyoki Mawia, Ju Luo, Peisong Hu

引用次数: 0

Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.11.002

He Zhenrui , Zhao Wenhua , Cheng Baoping , Yang Mei , Yang Yingqing , Zhu Yiming , Zhou Erxun

{"title":"Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens","authors":"He Zhenrui , Zhao Wenhua , Cheng Baoping , Yang Mei , Yang Yingqing , Zhu Yiming , Zhou Erxun","doi":"10.1016/j.rsci.2024.11.002","DOIUrl":"10.1016/j.rsci.2024.11.002","url":null,"abstract":"<div><div>Rice false smut (RFS), caused by the ascomycete fungus <em>Ustilaginoidea virens</em> (Cooke) Takahashi, is a notable panicle disease of rice. Mycoviruses represent a category of viruses capable of infecting various fungal groups, garnering interest for their potential application in the biological control of plant fungal diseases. We identified a novel positive-sense single-stranded RNA virus, named <em>Ustilaginoidea virens</em> mitovirus 1 (UvMV1), from an abnormal <em>U. virens</em> strain Uv488. The complete genome sequence of UvMV1 is 2 474 nt in length and contains a large open reading frame encoding RNA-dependent RNA polymerase. UvMV1 is classified as a new member of the genus <em>Unuamitovirus</em> in the family <em>Mitoviridae</em> based on phylogenetic analysis. It is effectively transmitted vertically through conidia of strain Uv488 and horizontally through hyphal fusion between vegetatively compatible individuals with an 80% transmission efficiency. We further demonstrated that UvMV1 significantly influenced conidiation, colony morphology, growth rate, secondary metabolite production, and the response to environmental stress in <em>U. virens</em>. Furthermore, qRT-PCR results aimed at detecting the expression levels of autophagy-related genes suggested that UvMV1 infection had the potential to induce autophagy in <em>U. virens</em>. Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in <em>U. virens</em>, while inhibition of autophagy results in decreased UvMV1 titers. Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated with secondary metabolism, mycelial growth, virulence, and the mitogen-activated protein kinase signaling pathway, thereby influencing both the antiviral response and the virulence of <em>U. virens</em>. The data presented collectively identified a novel mycovirus responsible for inducing growth abnormalities in <em>U. virens</em>, and elucidated the regulatory mechanisms during mycovirus-host fungus interactions, particularly the autophagy pathway. These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 243-258"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Discovery of Candidate Genes Associated with Cold Tolerance in Rice at Various Growth Stages

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.12.001

Jiajun Liu , Cuili Wang , Mingmao Sun , Xiaoding Ma , Bing Han , Xiaohong Guo , Longzhi Han , Di Cui

引用次数: 0

Progress on Physiological Mechanisms of Rice Spikelet Degeneration at Different Panicle Positions Caused by Abiotic Stress

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.09.002

Wang Jingqing , Wang Yaliang , Chen Yulin, Chen Huizhe, Xiang Jing, Zhang Yikai, Wang Zhigang, Zhang Yuping

{"title":"Progress on Physiological Mechanisms of Rice Spikelet Degeneration at Different Panicle Positions Caused by Abiotic Stress","authors":"Wang Jingqing , Wang Yaliang , Chen Yulin, Chen Huizhe, Xiang Jing, Zhang Yikai, Wang Zhigang, Zhang Yuping","doi":"10.1016/j.rsci.2024.09.002","DOIUrl":"10.1016/j.rsci.2024.09.002","url":null,"abstract":"<div><div>Rice yield is heavily reliant on the number of spikelets per panicle, a factor determined by the processes of spikelet differentiation and degeneration. In rice cultivars with large panicles, spikelet degeneration negates the advantages of large panicle and constrains yield potential. Environmental stress-induced metabolic disorders in plants aggravate spikelet degeneration, with the sensitive period for this process commencing approximately 15‒20 d before panicle heading. Notable positional variations occur within the panicle, with significantly higher spikelet degeneration rates at the basal than at the upper positions. An imbalance of carbon and nitrogen metabolism represents the primary physiological basis for aggravated spikelet degeneration under abiotic stress. Impaired carbon and nitrogen metabolism leads to disordered energy metabolism and disrupted respiratory electron transport, which accelerates the apoptosis of young spikelets through excessive reactive oxygen species accumulation. Sucrose serves as the main carbohydrate source for spikelet development, demonstrating an apical dominance pattern that favors spikelet formation. However, under abiotic stress, the inhibition of sucrose decomposition, rather than sucrose transport impairment, predominantly contributes to aggravated spikelet degeneration at the basal panicle positions. Brassinolide and auxin have a significant relationship with spikelet formation, potentially mediating apical dominance. Specifically, brassinolide enhances sucrose accumulation and utilization, thereby alleviating spikelet degeneration. At present, the mechanisms underlying rice spikelet degeneration have not been fully revealed, and the joint effects of hormones, carbohydrates, and carbon and nitrogen metabolism on this process require further investigation. To reduce the spikelet degeneration, the strategic application of water and fertilizer to establish a stable rice population can enhance the rice plants’ resilience to abiotic stress. An effective approach to reducing spikelet degeneration is to increase the dry matter occupancy of each spikelet during the panicle initiation period.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 193-202"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Approach for Screening Salinity-Tolerant Rice Germplasm by Exploring Redox-Regulated Cytological Fingerprint

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2025.03.006

Uthpal Krishna Roy, Babita Pal, Soumen Bhattacharjee

{"title":"A Novel Approach for Screening Salinity-Tolerant Rice Germplasm by Exploring Redox-Regulated Cytological Fingerprint","authors":"Uthpal Krishna Roy, Babita Pal, Soumen Bhattacharjee","doi":"10.1016/j.rsci.2025.03.006","DOIUrl":"10.1016/j.rsci.2025.03.006","url":null,"abstract":"<div><div>Although metabolic homeostasis disruption, cellular damage, and premature senescence caused by salinity stress are well-documented in the literature, there are few studies investigating cytological changes induced by salinity stress within the altered metabolic landscape of rice, and this study aims to fill that gap. The cytological characterization of root tips (in terms of mitotic index and chromosomal abnormalities such as stickiness, laggards, fragments, bridges, micronuclei, ring chromosomes, and total mitotic abnormalities) was conducted on 10 experimental rice landraces from coastal Bangladesh, grown under post-imbibitional salinity stress (PISS), while correlating these changes with their metabolic status. The results revealed a strong correlation between salinity-induced cytological changes in root cells (mitotic index and chromosomal abnormalities) and the redox interactome status of all experimental rice landraces. The landraces Kutepatnai, Talmugur, Nonakochi, and Benapol, which exhibited a higher ability to mitigate PISS-induced chromosomal abnormalities and improve mitotic index, also showed lower accumulation of oxidative stress markers (protein carbonylation, lipid peroxidation, prooxidant accumulation, oxidative stress index, reactive oxygen species (ROS)-antioxidative stress index, and efficiency of ROS processing via the Halliwell-Asada pathway) compared with more susceptible landraces (Charobalam, Jotaibalam, Kachra, and Lalmota). These findings underscore the role of redox biology in preventing chromotoxic effects under salinity stress. Hierarchical cluster analysis and principal component analysis, used to determine variations and similarities among the experimental rice landraces based on cytological attributes, redox interactome, and physiological phenotypes, classified the landraces according to their salinity tolerance and sensitivity. This study proposes a novel approach for exploring redox-regulated cytological fingerprints as a tool for identifying salinity-tolerant rice landraces.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 228-242"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Detection of Bacterial Pathogens in Rice by Recombinant Polymerase Amplification Based on Self-Heating Paper Chip

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2025.01.001

Ning Yang , Jiuju Fu , Aiying Wang , Shuhua Liu , Kangpeng Chang , Wei Song , Shoulong Tao , Hanping Mao , Xiaodong Zhang , Lijia Xu

引用次数: 0

Establishing Programmable CRISPR/Cas13b-Mediated Knockdown System in Rice

IF 5.6 2区农林科学

Rice Science Pub Date : 2025-03-01 DOI: 10.1016/j.rsci.2024.12.012

Wang Shuman , Zhang Linqi , Gao Ruiren , Wei Guangbo , Dong Weiguo , Xu Jiming , Wang Zhiye

{"title":"Establishing Programmable CRISPR/Cas13b-Mediated Knockdown System in Rice","authors":"Wang Shuman , Zhang Linqi , Gao Ruiren , Wei Guangbo , Dong Weiguo , Xu Jiming , Wang Zhiye","doi":"10.1016/j.rsci.2024.12.012","DOIUrl":"10.1016/j.rsci.2024.12.012","url":null,"abstract":"<div><div>CRISPR-Cas endonucleases mediate prokaryotic adaptive immunity by targeting foreign nucleic acids. CRISPR/Cas13b is a class 2 type VI-B ribonuclease that targets and cleaves single-stranded RNA. It exhibits higher RNA interference activity than Cas13a and Cas13c and causes fewer collateral effects than RxCas13d in mammalian cells. However, a programmable CRISPR/Cas13b-mediated RNA interference system for endogenous transcripts in rice has not yet been established. Here, we developed a CRISPR/Cas13b-mediated system to target endogenous transcripts in rice. Our CRISPR/Cas13b system could inhibit multiple endogenous mRNAs simultaneously. In addition, this system efficiently repressed endogenous long noncoding RNAs with more than 50% inhibition in stable transgenic plants. Furthermore, we found only weak collateral effects of the CRISPR/Cas13b-mediated system at the transcriptome-wide level, and no difference in the agronomic traits of stable transgenic rice in the field. We present a programmable CRISPR/Cas13b-mediated knockdown system for rice, offering a potential biotechnological tool for functional genomics and crop improvement.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"32 2","pages":"Pages 217-227"},"PeriodicalIF":5.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0