Rice Science最新文献

{"title":"Microbial Insecticides for Rice Insect Pest Management","authors":"Xinxin ZHANG,&nbsp;Guoqing CHEN,&nbsp;Guozhong FENG","doi":"10.1016/j.rsci.2026.04.003","DOIUrl":"10.1016/j.rsci.2026.04.003","url":null,"abstract":"<div><div>Microbial pesticides are being increasingly adopted in modern agriculture as sustainable alternatives to synthetic chemical pesticides, featuring outstanding target specificity and low environmental risk. Their application plays a vital role in rice production, enabling effective suppression of key rice insect pests including stem borers, leaf folders, and planthoppers, while preserving ecological balance. The research, development, and popularization of microbial insecticides facilitate green agricultural development, with numerous commercial formulations successfully launched worldwide. This review summarizes the research progress of microbial insecticides categorized as bacterial, fungal, viral, and metabolite-based agents, focusing on their practical application in rice insect pest control. Furthermore, it discusses prominent technical and practical bottlenecks restricting their large-scale promotion and prospects future development directions consistent with sustainable plant protection strategies.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 438-448"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MDBP: An Integrated Web-Based Platform for Accelerating Mutagenesis-Directed Breeding","authors":"Yan PENG,&nbsp;Bigang MAO,&nbsp;Wenbang TANG,&nbsp;Bingran ZHAO,&nbsp;Ye SHAO","doi":"10.1016/j.rsci.2026.04.006","DOIUrl":"10.1016/j.rsci.2026.04.006","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 411-414"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of Vitamins and Minerals in Coated and Extruded Fortified Rice Kernels Stored under Real-Life Conditions over Two Years and Implications for Standards","authors":"Werner NADER,&nbsp;Deblina SARKAR,&nbsp;Melas ADOKO,&nbsp;Saskia de PEE,&nbsp;Elise IVARSEN,&nbsp;Vitalii SHKLIAR,&nbsp;Sabine Meng JENSEN,&nbsp;Johannes HEIN","doi":"10.1016/j.rsci.2026.03.007","DOIUrl":"10.1016/j.rsci.2026.03.007","url":null,"abstract":"<div><div>Rice fortification is a cost-effective intervention to address and prevent micronutrient deficiencies in contexts where rice is a staple food. The stability of such added micronutrients is of critical importance, especially when rice is stored under harsh storage conditions. Coated and extruded fortified rice kernels (FRKs) containing vitamins A, B1, B3, B6, B9, and B12, zinc, and iron, as well as bulk rice fortified with these kernels, were stored in different packaging materials over periods of 24 and 26 months, respectively. Blending, final packaging, and storage were conducted in Madhya Pradesh, in central India, which experiences a subtropical monsoon climate condition. Large variations in the analytical results were observed for the fortified rice samples, mostly due to non-homogeneous distribution of the FRKs within the bulk rice. Consequently, to reduce analytical variation, results were correlated with the concentration of vitamin B3 as an internal standard. Vitamins B3, B6, B9, and B12, zinc, and iron remained stable in both coated and extruded FRKs throughout storage, while vitamin B1 stability was higher in coated FRKs, with results consistent across packaging materials. The decay of vitamin B1 followed zero-order kinetics in extruded premix kernels stored in metallized paper bags and in rice fortified with these kernels across all packaging types, with half-lives of 20 and 34 months, respectively. Vitamin A decay followed first-order kinetics in both rice fortified with coated and extruded kernels, with half-lives of 6.8 and 15.1 months, respectively. In FRKs, vitamin A decay was best described by mixed-order kinetics, with half-lives of 6.5 months (coated) and 5.5 months (extruded). This study informed major revisions to the World Food Programme fortified rice specifications, including the removal of vitamin A due to its instability during storage, while confirming the stability of B vitamins, zinc, and iron, and highlighting the critical role of blending and analytical control practices.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 559-570"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Line Hybrid Rice in China: Fifty Years of Sustained Improvement in Yield, Quality, and Stress Resistance","authors":"Junyi GONG,&nbsp;Xiaobo ZHANG,&nbsp;Jianfu ZHANG,&nbsp;Bo ZENG,&nbsp;Xiaoqing ZHANG,&nbsp;Xia XU,&nbsp;Benyi CHENG,&nbsp;Yuxuan HOU,&nbsp;Junhui XIA,&nbsp;Jianli WU,&nbsp;Shihua YANG,&nbsp;Shihua CHENG,&nbsp;Bin HAN,&nbsp;Huaan XIE","doi":"10.1016/j.rsci.2026.04.004","DOIUrl":"10.1016/j.rsci.2026.04.004","url":null,"abstract":"<div><div>China pioneered the global commercial application of hybrid rice (<em>Oryza sativa</em> L.). Chinese scientists not only proved that heterosis can be realized in self-pollinating crops, a groundbreaking theoretical advance, but also developed commercial hybrid rice breeding into one of China’s most sophisticated agricultural technology models, making outstanding contributions to national food security. This study systematically reviews the 50-year developmental trajectory of officially approved three-line hybrid rice varieties at national and provincial levels in China, through analyzing the approval statistics of individual restorer lines and male sterile lines, the dynamic changes in the popularized planting area of major varieties, and the phenotypic variation in core agronomic traits including grain yield, grain quality, and disease/pest resistance. The paper comprehensively summarizes the remarkable progress, major achievements and practical experience accumulated over half a century of three-line hybrid rice breeding in China, and prospects future research directions for this system.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 499-513"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeding Rice to Recruit Diazotrophs: Plant Genetic Levers for Rhizosphere Nitrogen Fixation","authors":"Qingnan CHU,&nbsp;Detian LI,&nbsp;Zhihang FENG,&nbsp;Mengcen WANG,&nbsp;Zhimin SHA","doi":"10.1016/j.rsci.2026.04.005","DOIUrl":"10.1016/j.rsci.2026.04.005","url":null,"abstract":"<div><div>Synthetic nitrogen (N) fertilizers sustain modern rice yields but cause substantial environmental impacts due to low nitrogen-use efficiency. Flooded paddy soils harbor diverse diazotrophs capable of biological nitrogen fixation (BNF), yet BNF is highly variable across soils, management practices, and rice genotypes. In this review, we synthesize recent evidence on how rice can be bred to more consistently recruit and stimulate N₂-fixing microbiomes. We first summarize major diazotroph taxa and niches in paddy ecosystems, highlighting the emerging contribution of iron-reducing bacteria at root iron-plaque interfaces and the principal environmental ‘gates’ on BNF, including flooding regime, bioavailable iron (Fe) phases, pH, carbon (C) quality, and mineral-N inputs. We then integrate findings showing that rice genetic variation shapes diazotroph assembly and activity through four root-controlled levers: (i) root system architecture that positions rhizodeposition along redox gradients, (ii) exudate quantity and chemistry (notably flavonoids and low-molecular-weight organic acids) that fuel and signal to diazotrophs, (iii) aerenchyma-mediated radial O₂ loss that creates oxic-anoxic microsites, and (iv) iron plaque formation that couples Fe-C-N cycling and provides a scaffold for N₂-fixing communities. Finally, we translate these mechanisms into a breeding roadmap, proposing a BNF-supportive ideotype, candidate loci/genes from genome-wide association study (GWAS)/QTL and wild introgressions, and a validation-to-deployment pipeline combining gene editing, near-isogenic resources, marker-assisted/genomic selection, and multi-environment field testing under low-N management. We also discuss phenotyping bottlenecks, deployment constraints, and priorities for pairing BNF-supportive alleles with compatible microbiomes and agronomy practices to reduce fertilizer demand while maintaining yield.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 465-484"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and Establishment of Soil Health Evaluation Framework in Black Soil Region Based on Multifunctionality: A Case Study of Fangzheng County, Heilongjiang Province, China","authors":"Zhuoting HE,&nbsp;Yu JIANG,&nbsp;Raza ALI,&nbsp;Qingxu MA,&nbsp;Xiao LI,&nbsp;Haimin KONG,&nbsp;Yali KONG,&nbsp;Lianfeng ZHU,&nbsp;Chunquan ZHU,&nbsp;Wenhao TIAN,&nbsp;Lianghuan WU,&nbsp;Junhua ZHANG,&nbsp;Qianyu JIN,&nbsp;Chao MA,&nbsp;Xiaochuang CAO","doi":"10.1016/j.rsci.2026.03.008","DOIUrl":"10.1016/j.rsci.2026.03.008","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 420-424"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction Effect of Chelerythrine on Apoptosis of Sf9 Cells: A Preliminary Investigation Based on Cell Morphology and Activity","authors":"Qinghui WEI,&nbsp;Zhenghao SHI,&nbsp;Zhiyong LI,&nbsp;Yaqing PAN,&nbsp;Weifeng SONG,&nbsp;Chunlai LIU","doi":"10.1016/j.rsci.2026.04.012","DOIUrl":"10.1016/j.rsci.2026.04.012","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 435-437"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing Green-Leaf Black Rice Germplasm by Knockout of OsC1 Gene","authors":"Fuhao LI,&nbsp;Liyun GE,&nbsp;Peina WANG,&nbsp;Wenyu SUN,&nbsp;Nuo GONG,&nbsp;Yingchun HAN,&nbsp;Chunbo MIAO,&nbsp;Zhen CHEN,&nbsp;Yanxiu DU,&nbsp;Hongzheng SUN,&nbsp;Junzhou LI","doi":"10.1016/j.rsci.2026.03.004","DOIUrl":"10.1016/j.rsci.2026.03.004","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 415-419"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyruvate Orthophosphate Dikinase B (PPDKB) Deficiency Impairs Starch Biosynthesis and Redirects Carbon Flux to Lipid and Amino Acid Synthesis in Rice","authors":"Bowen DENG,&nbsp;Yining YING,&nbsp;Jianming PAN,&nbsp;Tongrui ZHANG,&nbsp;Feifei XU,&nbsp;Jinsong BAO","doi":"10.1016/j.rsci.2026.04.010","DOIUrl":"10.1016/j.rsci.2026.04.010","url":null,"abstract":"<div><div>The central endosperm of a chalky rice mutant <em>JM03</em>, which was isolated from a ⁶⁰Co-irradiated mutant population of the <em>indica</em> rice variety 93-11, contains numerous small, irregularly shaped starch granules with looser packing than those of the wild type (WT). <em>JM03</em> starch showed lower gelatinization onset and peak temperatures, reduced pasting viscosities, and consistently decreased dynamic rheological parameters compared with WT. Through bulked segregant analysis sequencing (BSA-seq), complementation tests, and immunoblot analysis, we identified <em>PPDKB</em> (<em>Os05g0405000</em>) as the causal gene underlying the <em>JM03</em> phenotype. Multi-omics analysis of developing endosperm at 15 d after flowering revealed that PPDKB deficiency profoundly redirected central carbon and amino acid metabolism. On the one hand, impaired phosphoenolpyruvate regeneration disrupted the gluconeogenic conversion of hexoses into starch biosynthesis precursors, consequently suppressing starch accumulation through downregulation of all starch synthesis-related genes and enzymes. The impaired starch synthesis diminished sucrose unloading capacity due to reduced expression of sucrose synthase, triggering accumulation of sucrose and other soluble sugars. On the other hand, excess pyruvate diverted metabolic flux towards acetyl-CoA production, stimulating tricarboxylic acid (TCA) cycle activity and enhancing lipid biosynthesis. Meanwhile, amino acid synthesis was enhanced due to increased levels of multiple precursors and genes/enzymes involved in this process. The reduction in starch accumulation, combined with the downregulation of key grain weight regulators, such as mitogen-activated protein kinase 6 and BAHD acyltransferase-like protein, collectively led to a significant reduction in grain weight in <em>JM03</em>. Taken together, our study reveals a functional cross-talk between starch, soluble sugars, protein, and lipids in rice endosperm during seed development in <em>JM03</em>, which provides important germplasm resources and a theoretical basis for genetic improvement of rice yield and quality.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 514-530"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chloroplast-Derived Reactive Oxygen Species Dynamics Mediate Jasmonic Acid-Induced Drought Tolerance in Rice","authors":"Jiwoong JUNG,&nbsp;Deok Hyun SEO,&nbsp;Youngdae YOON,&nbsp;Geupil JANG","doi":"10.1016/j.rsci.2026.03.005","DOIUrl":"10.1016/j.rsci.2026.03.005","url":null,"abstract":"<div><div>Beyond their central role in photosynthesis and plant productivity, chloroplasts are emerging as key regulators of plant responses and tolerance to abiotic stress. However, the molecular mechanisms linking chloroplastic reactive oxygen species (ROS) metabolism to stress adaptation remain poorly understood. Here, we show that knockout of <em>OsJAZ9</em>, a repressor of jasmonic acid (JA) signaling, enhances JA response and drought tolerance in rice by suppressing cellular ROS accumulation. Time-lapse visualization of subcellular ROS dynamics revealed that stress-induced ROS accumulation was initiated in chloroplasts and subsequently propagated to the cytoplasm. Notably, loss of <em>OsJAZ9</em> markedly suppressed chloroplastic ROS accumulation, resulting in reduced cellular ROS levels under stress conditions. Consistently, either JA treatment or overexpression of the JA-responsive chloroplastic ROS scavenger <em>OsFeSOD3</em> was sufficient to attenuate chloroplastic ROS accumulation and enhance drought tolerance in rice. Furthermore, two-year agronomic field analyses showed that <em>osjaz9</em> knockout rice exhibited improved grain yield, particularly under drought stress conditions. Together, our findings identify chloroplast-derived ROS dynamics as a pivotal molecular link between JA signaling and rice stress tolerance and highlight <em>OsJAZ9</em> as a promising molecular target for developing high-yielding, stress-tolerant rice cultivars.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"33 4","pages":"Pages 531-544"},"PeriodicalIF":7.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148718626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}