Science Progress最新文献

{"title":"Comparison of the postoperative analgesic efficacy of pericapsular nerve group block (PENG) and anterior quadratus lumborum block in hip fracture surgery: A prospective randomized study.","authors":"Serpil Şehirlioğlu, Döndü G Moralar","doi":"10.1177/00368504251382032","DOIUrl":"10.1177/00368504251382032","url":null,"abstract":"<p><p>ObjectiveIn recent years, the pericapsular nerve group (PENG) block and anterior quadratus lumborum block (aQLB) have emerged as regional anesthesia techniques commonly used for pain control after hip surgery. This study compared their analgesic efficacy during the first 48 hours following surgery under spinal anesthesia.MethodsIn this prospective, randomized, single-blinded study, patients were assigned to either the PENG (<i>n</i> = 43) or the aQLB group (<i>n</i> = 30). The primary outcome of the study was the total tramadol consumption within the first 48 hours postoperatively. Secondary outcomes included time to first rescue analgesia, resting and dynamic NRS pain scores at 2, 12, 24, and 48 hours postoperatively, and the incidence of complications.ResultsIn this study, postoperative tramadol consumption within the first 48 hours was significantly lower in the PENG (96.74 ± 77.36 mg) compared to the aQLB group (196.33 ± 157.43 mg) (<i>p</i> = 0.004). Additionally, the time to first rescue analgesia was significantly longer in the PENG (9.03 ± 7.67 hours) than in the aQLB group (6.81 ± 6.70 hours) (<i>p</i> = 0.048). NRS scores were similar between the groups at all time points. Quadriceps weakness was not observed in any patient.ConclusionThe PENG block provides effective postoperative analgesia without causing motor blockade, making it advantageous for early mobilization in patients undergoing hip surgery. It can be used as a part of multimodal analgesia.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251382032"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors of thrombocytopenia in adults receiving extracorporeal membrane oxygenation support: A single center retrospective cohort study.","authors":"Hongjie Tong, Jiali Yao, Feiyan Pan, Kaixuan Wang, Jin Jinjin, Qianqian Wang, Kun Chen","doi":"10.1177/00368504251385949","DOIUrl":"10.1177/00368504251385949","url":null,"abstract":"<p><p>ObjectivesTo investigate the risk factors associated with thrombocytopenia in patients undergoing extracorporeal membrane oxygenation (ECMO) support.MethodsThis retrospective cohort study included 230 patients who received ECMO support during January 2018 to December 2024. Clinical data collected included age, gender, causes of ECMO, medical history, vital signs, laboratory test results, complications and outcomes. The patients were divided into the thrombocytopenia group and the control group based on whether the patient's platelet count was less than 150*10⁹/L. The differences in clinical data between the two groups were compared. Moreover, risk factors associated with thrombocytopenia were identified using univariate and multivariate regression analyses.ResultsIn this study, a total of 230 patients undergoing ECMO were involved. The mean age of patients was 52 ± 8 years with 119 (51.7%) males and 111 (48.3%) females. The age, sex distribution, reasons for ECMO, previous medical history, temperature, respiration rate, heart rate, mean arterial pressure, white blood cells, hemoglobin, C-reactive protein, platelets, total bilirubin, alanine aminotransferase, aspartate aminotransferase, prothrombin time, activated partial thromboplastin time, blood lactate between two groups showed no statistically significant differences (<i>P</i> > 0.05) while the APACHE II score (24(20,28) vs 21(17,24)) was higher in thrombocytopenia group (<i>P</i> < 0.05). The bleeding events were significantly higher (62.4% vs 15.7%), in-hospital survival (49.7% vs 62.9%) was lower, the duration of ICU stay (19(16,23) vs 18 (14,22)) was longer, and more platelet (30(10,40) vs 15 (10,20)) and plasma transfusions (600(400,800) vs 450 (200,600)) in thrombocytopenia group (<i>P</i> < 0.05). Logistic regression analysis showed that APACHE II score, rotational speed over the first 48 h, with sepsis, the heparin doseage before ECMO, use of continuous renal replacement therapy (CRRT) and intra-aortic balloon pump (IABP) during ECMO were risk factors associated with thrombocytopenia.ConclusionsThe incidence of thrombocytopenia in patients receiving ECMO is high and may be associated with poor prognosis. Patients with higher APACHE II score, higher rotational speed during the first 48 h, sepsis, higher heparin doseage before ECMO initiation, and the use of CRRT or IABP may at an increased risk of developing thrombocytopenia.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251385949"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and validation of a risk prediction model for intraoperative hypothermia in children undergoing thoracoscopic lobectomy.","authors":"Xiongtao Liu, Hua Lin, Yanzhen Li, Chunli Dong, Ting Wang, Xia Wang, Qiqi Yan, Ruzhong Liu, Liyan Zhao, Juan Xiao, Xiaohui Gou","doi":"10.1177/00368504251386310","DOIUrl":"https://doi.org/10.1177/00368504251386310","url":null,"abstract":"<p><p>To develop a predictive model to assess the risk of peri-operative hypothermia occurrence in children undergoing thoracoscopic lobectomy and validate its effectiveness. 172 children who underwent thoracoscopic lobectomy were randomly divided into a training set of 124 cases and a validation set of 48 cases in a 7:3 ratio. The study found a 33.1% incidence of intraoperative hypothermia (IPH) in children undergoing thoracoscopic lobectomy. Logistic regression analysis was performed using SPSS 26.0 to screen influencing factors, establish a risk prediction model, and draw the receiver-operating characteristic curve. Univariate analysis identified anesthesia-skin incision time, operating-room temperature, humidity, basal body temperature, end body temperature, and body temperature half an hour after anesthesia as influencing factors. Multivariate logistic regression revealed anesthesia-skin incision time (odds ratio (OR) = 1.595), operating-room humidity (OR = 4.094), and body temperature half an hour after anesthesia (OR = 112.595) as independent predictors. The nomogram model demonstrated an excellent discrimination with area under the curve (AUC) of 0.989 (95% confidence interval (CI): 0.976-1.000), maximum Youden index of 0.94, sensitivity of 1, specificity of 0.94, and cutoff value of 0.195. The Hosmer-Lemeshow test (<i>χ</i>₂ = 1.751, P = 0.195) and bootstrap resampling (consistency coefficient = 0.947) confirmed the model's goodness of fit, internal consistency, and stability. Validation set results are similar to those in the training set, with an AUC of 0.989 (95% CI: 0.969-1.000), a sensitivity of 1, a specificity of 0.929, and a calibration curve error of 0.032 (<0.05), indicating high predictive accuracy. These findings suggest nomogram is a robust tool for predicting IPH in the pediatric thoracoscopic lobectomy.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251386310"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green remediation: <i>Casuarina equisetifolia</i> fruit-based activated carbon for pharmaceutical removal.","authors":"Alaa M Al-Ma'abreh, Ethar M Al-Essa, Dareen A Hmedat, Gada Edris, Fida' F Odeh, Mariam Hamed","doi":"10.1177/00368504251382003","DOIUrl":"10.1177/00368504251382003","url":null,"abstract":"<p><p>This work utilized <i>Casuarina equisetifolia</i> fruit-based activated carbon (CEAC) to investigate the simultaneous removal of paracetamol (PA), caffeine (CAF), and acetylsalicylic acid (AS) from aqueous solutions. The efficacy of adsorbent removal was investigated using adsorption factors such as pH, CEAC amount, initial pharmaceutical concentration, and adsorption period. Fourier-transform infrared spectroscopy, scanning electron microscopy, and UV-visible analysis confirmed the simultaneous adsorption of the pharmaceutical mixture onto the CEAC surface. The results showed a slight effect of pH on the simultaneous adsorption efficiencies with a CEAC dose of 110 mg. The ideal initial concentration for the pharmaceutical mixture was determined to be 15 mg L^-1. The perfect contact duration for the simultaneous removal of the three pharmaceuticals was found to be 30 min. Kinetic tests demonstrated that the pharmaceutical mixture adsorbs using a pseudo-second-order process. The Freundlich isotherm model describes the equilibrium data. The maximum adsorption capabilities were PA (84.15.23 mg g^-1), CAF (79.16 mg g^-1), and AS (61.32 mg g^-1). A thermodynamic study reveals that these adsorption processes are endothermic and spontaneous. Finally, regeneration experiments confirm the reusability of CEAC.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251382003"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of cochlear implantation in patients with unilateral sensorineural hearing loss with and without bony cochlear nerve canal stenosis.","authors":"Xinmiao Fan, Wei Gu, Tengyu Yang, Fuyu Zhang, Ruzhen Gao, Yue Fan, Xiaowei Chen","doi":"10.1177/00368504251386315","DOIUrl":"https://doi.org/10.1177/00368504251386315","url":null,"abstract":"<p><p>ObjectiveTo evaluate outcomes of cochlear implantation (CI) in patients with unilateral sensorineural hearing loss (USNHL) and explore effects and stimulation current in USNHL patients with bony cochlear nerve canal (BCNC) stenosis versus normal cochlear nerve canals.MethodsA prospective study was conducted on 23 USNHL patients who underwent CI (MED-EL) from May 2019 to March 2023. Preoperative CT and MRI scans were performed to measure BCNC width. Subjects were divided into BCNC stenosis (BCNC-S) and BCNC normal (BCNC-N) groups. Hearing thresholds, speech recognition scores (SRS), sound localization ability, and auditory/musical abilities were assessed before and two years after CI. Differences were determined using Stata statistical software, with significance defined as <i>p</i> < .05.ResultsEleven patients were diagnosed with BCNC stenosis. CI significantly improved SRS under noisy conditions (<i>p</i> = .027) and sound localization ability (<i>p</i> < .05), as well as auditory and musical quality (<i>p</i> < .05). No significant differences in outcomes were found between BCNC-S and BCNC-N groups. BCNC-S group had higher stimulation current than BCNC-N group.ConclusionsCI provides similar improvements for USNHL patients with or without BCNC stenosis.SignificanceThe findings will contribute to the understanding of cochlear implant outcomes in patients with USNHL and BCNC stenosis.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251386315"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comorbidome and community-acquired pneumonia: Analysis by age, sex, and intensive care unit admission.","authors":"Juan Sebastian Hernández Puentes, Alirio Rodrigo Bastidas-Goyes, Eduardo Andres Tuta Quintero, Diana Marcela Díaz Quijano, Lina María López Núñez, Juan Diego Castro Córdoba, María Paula Vásquez Bazurto, Catalina Marenco Galvis, Juanita Fetecua Chaparro, Juan Miguel Moreno Orozco, Pablo Sobrino Montoya, Isabella Criado Quintero, Laura Valentina Medellín Ortiz, Tomás Salamanca España, Alejandra Mora Vega, Valeria Leyton Franco, Cristhian Fabian Acero Murillo","doi":"10.1177/00368504251384203","DOIUrl":"10.1177/00368504251384203","url":null,"abstract":"<p><p>ObjectiveTo evaluate the influence of age, sex, and the need for intensive care unit admission on the clinical outcomes of patients with community-acquired pneumonia (CAP), considering the impact of comorbidities such as cardiovascular diseases, chronic kidney disease, and oncological conditions on the risk of complications and mortality.MethodologyA multicenter retrospective cohort study was conducted in patients with CAP admitted to two tertiary-level institutions in Colombia between January 2010 and December 2020. A comorbidity map (comorbidome) was developed to reflect the prevalence of these conditions according to intensive care unit (ICU) stay, sex, and age.ResultsIn a cohort of 3370 patients, the most frequent comorbidities were arterial hypertension (50.1%), anemia (31.8%), and chronic pulmonary disease (27.8%). Functional dependency showed the strongest association with mortality (OR 7.17; 95% CI 5.6-9.17), with significant differences in distribution and strength of association according to age, sex, and need for ICU admission.ConclusionThis study demonstrated the high prevalence of comorbidities in patients with CAP in Colombia, highlighting arterial hypertension, anemia, and chronic pulmonary disease as the most common. The distribution of these comorbidities varied according to age and sex, although a very similar clinical profile was observed regarding the need for ICU admission.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251384203"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel diagnostic approach to differentiate iron overload from inflammation in children using transferrin saturation (TSAT) and ferritin-based indices: A cross-sectional study.","authors":"Gustavo F Gonzales, Julio Aguilar, Sandra Yucra, Cinthya Vásquez-Velásquez","doi":"10.1177/00368504251385071","DOIUrl":"https://doi.org/10.1177/00368504251385071","url":null,"abstract":"<p><p>ObjectiveTo evaluate the TSAT*SF and SF/TSAT indices in children living at different altitudes, with and without inflammation, and to examine their correlations with markers of iron homeostasis.MethodsA cross-sectional, multicenter study included 238 children aged 6 to 72 months. We assessed TSAT*SF and SF/TSAT indices in relation to serum hepcidin, total body iron (TBI), hematological and nutritional parameters, and inflammatory markers.ResultsChildren with elevated inflammatory markers resided at high altitude (HA) and exhibited increased hemoglobin (Hb), hematocrit, and a higher prevalence of erythrocytosis, alongside lower rates of anemia. Log-transformed values of SF, TBI, hepcidin, SF/TSAT ratio, and log(SF/TSAT) were significantly elevated in the inflammation group. In contrast, TSAT*SF values were unaffected by inflammation, showing no significant differences between groups. Among children with normal C-reactive protein (CRP ≤10 mg/L), TSAT*SF > 900 was associated with higher Hb, serum iron, hepcidin, TBI, and TSAT, as well as reduced height-for-age and weight-for-age Z-scores. Across the full sample, CRP positively correlated with ferritin, hepcidin, TBI, neutrophils, and unadjusted Hb, and negatively with serum iron and lymphocytes. CRP showed no correlation with TSAT*SF but was positively associated with the SF/TSAT ratio. Multivariate analysis revealed that the TSAT*SF index correlated with iron status but not inflammation, whereas the SF/TSAT index correlated with both iron status and inflammation. The SF*TSAT ratio demonstrated differential performance, showing a higher area under the ROC curve for the diagnosis of iron deficiency compared to inflammation (0.75 vs 0.98, <i>p</i> <  0.05), whereas the log-transformed SF/TSAT ratio did not exhibit this distinction.ConclusionThe TSAT*SF index effectively identifies tissue iron overload independently of inflammation. Its use may improve iron status assessment in pediatric populations exposed to HA and systemic inflammation. Elevated TSAT*SF was linked to erythrocytosis and impaired growth, underscoring its diagnostic and public health significance.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251385071"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145260138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a predictive model of intravenous calcium supplementation needs following total parathyroidectomy in patients with secondary hyperparathyroidism.","authors":"Ting Bi, Luyuan Jin, Sijia Bai, Guangming Cheng, Shuai Guo, Xiaodong Feng, Wei Zhang","doi":"10.1177/00368504251386312","DOIUrl":"10.1177/00368504251386312","url":null,"abstract":"<p><p>ObjectiveTo develop a predictive model of intravenous calcium supplementation needs in patients with secondary hyperparathyroidism (SHPT) after total parathyroidectomy (t-PTX).MethodsA retrospective analysis was conducted on data from 550 SHPT patients treated at the Department of Hepatobiliary Pancreatic Spleen Thyroid Surgery, General Hospital of Northern Theater Command, from January 2015 to December 2022. Various biochemical indices of the patients were evaluated, including sex, serum ferritin (SF), alkaline phosphatase (AKP), and free thyroxine (FT4). Statistical tests, such as <i>t</i>-tests, were performed to identify significant risk factors associated with the need for postoperative intravenous calcium supplementation.ResultsSex (<i>t</i> = -1.988, <i>p</i> = 0.049), SF (<i>t</i> = 2.155, <i>p</i> = 0.033), AKP (<i>t</i> = 4.501, <i>p</i> = 0.000), and FT4 (<i>t</i> = -3.128, <i>p</i> = 0.002) were identified as risk factors associated with the need for postoperative intravenous calcium supplementation. The developed predictive model (intravenous calcium supplementation = 1235.561-158.547*gender + 0.141*SF + 0.442*AKP-494.055*FT4; male: 0, female: 1) was effective and passed the <i>F</i> test (<i>F</i> = 22.034, <i>p</i> = 0.000).ConclusionsThe developed predictive model provides a reliable tool for estimating the amount of intravenous calcium supplementation needed by SHPT patients following t-PTX. By incorporating sex, SF, AKP, and FT4, clinicians can make informed decisions and tailor individualized calcium supplementation schemes. This evidence-based approach may contribute to improved patient outcomes and optimize postoperative care for SHPT patients undergoing t-PTX. Further validation and application of the model are warranted to enhance its clinical utility.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251386312"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of extracorporeal membrane oxygenation in severe cardiotoxic poisoning: Analysis of a cohort over 10 years.","authors":"Baptiste Compagnon, Elsa Tardif, Vincent Pey, Bertrand Marcheix, François Labaste, Jean-Marie Conil, Vincent Minville, Fanny Vardon-Bounes","doi":"10.1177/00368504251358951","DOIUrl":"10.1177/00368504251358951","url":null,"abstract":"<p><p>ObjectiveThe objectives of this study were to identify early risk factors of circulatory assistance by venoarterial extracorporeal membrane oxygenation (VA-ECMO) on admission to intensive care unit (ICU) in population with severe cardiotoxic poisoning and to compare group characteristics with and without assistance.MethodAll patients included had severe clinical cardiotoxic poisoning, defined by hemodynamic failure requiring treatment with one or more catecholamines, in order to identify factors for circulatory assistance by multivariable logistic regression using bootstrap and supervised hierarchical classifiers methods. This single-center and retrospective study was carried out over 10 years from January 2012 to February 2022. We excluded all the patients with benign poisoning.ResultsOne-hundred forty-six cases were enrolled, including 24 with VA-ECMO (16.4%) and 122 with conventional treatment (83.6%). In circulatory support group, mortality, pneumoniae rate, intensive care length of stay and duration of mechanical ventilation were higher than conventional treatment group. Predictive factors for VA-ECMO on admission were mean arterial pressure ≤ 60 mmHg, lactatemia > 4.1 mmol/L and left ventricular ejection fraction ≤ 20%. The estimation of the classification and regression tree analysis was 91.1%.ConclusionsPatients admitted for severe cardiotoxic poisoning presenting with hypotension, hyperlactatemia, and left ventricular dysfunction were more frequently treated with extracorporeal life support, consistent with clinical severity. Early identification of such high-risk profiles is essential to prioritize timely referral to expert centers for optimal management.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251358951"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications for cystic fibrosis therapy: Potentiator icenticaftor is superior to ivacaftor in improving function and maintaining stability of F508del CFTR.","authors":"Deborah M Cholon, Luba A Aleksandrov, Nancy L Quinney, Susan E Boyles, Timothy J Jensen, Andrei A Aleksandrov, Martina Gentzsch","doi":"10.1177/00368504251384892","DOIUrl":"10.1177/00368504251384892","url":null,"abstract":"<p><p>ObjectiveThe objective of this study was to compare CFTR potentiators icenticaftor versus ivacaftor (IVA) to elucidate efficacy in augmenting CFTR function while preserving CFTR protein levels.MethodsSingle-channel measurements of CFTR in the presence and absence of IVA or icenticaftor were performed with membrane vesicles from BHK-21 cells stably expressing CFTR. CFTR-expressing BHK-21 cells and non-cystic fibrosis (CF) and CF primary human bronchial epithelial cultures were treated for 48 hours with elexacaftor/tezacaftor (ELX/TEZ) plus IVA or icenticaftor and then western blot analyses were performed to assess CFTR protein maturation. Non-CF and CF primary human bronchial epithelial cultures treated with CFTR modulators were subjected to Ussing chamber analysis to evaluate CFTR functional rescue upon 48-hour treatment and acute potentiator exposure.ResultsCorrector-rescued F508del CFTR displayed increased function with icenticaftor compared to IVA by single-channel measurements. In primary F508del cultures, 48-hour treatment with ELX/TEZ plus icenticaftor led to increased CFTR function in Ussing chambers compared to treatment with ELX/TEZ plus IVA. Western blot analysis demonstrated that F508del was destabilized by IVA but not icenticaftor. Primary N1303K CFTR cultures did not exhibit enhanced rescue with icenticaftor when compared to IVA, indicating that different CFTR mutations respond differently to potentiators.ConclusionIcenticaftor is superior to IVA as a potentiator for ELX/TEZ-rescued F508del CFTR, as 48-hour treatment with icenticaftor enhanced F508del function but did not destabilize F508del. Understanding the mechanisms underlying CFTR potentiator activities may offer further benefits for people with CF who have F508del or other CFTR mutations.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 4","pages":"368504251384892"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145240457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}