Science Progress最新文献

{"title":"Comprehensive high-throughput sequencing analysis and verification of miRNAs isolated from bronchial epithelial cells undergoing radiation-induced epithelial-mesenchymal transition.","authors":"Yifan Hou, Shenghui Zhou, Ping Wang, Duo Wang, Yuhao Liu, Xingkun Ao, Xinxin Liang, Jiaojiao Zhu, Ziyan Yan, Xiaochang Liu, Lin Zhou, Huixi Chen, Yan Zeng, Yongqing Gu","doi":"10.1177/00368504251362376","DOIUrl":"10.1177/00368504251362376","url":null,"abstract":"<p><p>Radiation-induced pulmonary fibrosis (RIPF) constitutes a significant complication in radiotherapy for various cancers, often severely limiting its efficacy. Recent studies suggest that epithelial-mesenchymal transition (EMT) plays a vital role in the pathogenesis of RIPF. Elucidating the involvement of microRNAs (miRNAs) in EMT could provide valuable insights into the mechanisms underlying RIPF and potentially reveal therapeutic targets. In this study, twelve dishes of BEAS-2B cells were irradiated with 6 Gy ⁶⁰Co γ-rays, and RNA was extracted at 0, 6, and 48 h after irradiation. High-throughput sequencing analysis of miRNA samples revealed miRNA significant changes in the BEAS-2B cells after irradiation, which was verified by RT-PCR. Additionally, the upstream transcription factors (TFs) were predicted through graphene oxide-based analysis. Transcription factors regulate the expression and transcriptional levels of miRNAs, and their functions may be associated with inflammatory or oxidative stress responses. The functional roles of these TFs were characterized through gene ontology (GO) analysis. Overall, we successfully screened and identified a set of miRNAs associated with ionizing radiation-induced EMT in lung epithelial cells and performed predictive identification of their upstream TFs and downstream regulatory target proteins. These data provide a firm foundation for future studies of the mechanism of radiation-induced EMT processes and related changes in biological function.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251362376"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated computation for 2D isogeometric acoustic model with ground reflection by integrating Taylor expansion and deep neural network.","authors":"Jinfeng Gao, Hehong Ma, Dongqing Miao, Ruxian Yao, Yu Zhang","doi":"10.1177/00368504251357783","DOIUrl":"10.1177/00368504251357783","url":null,"abstract":"<p><p>This article provides a method that combines Taylor expansion and neural network technology to accelerate the solution of an isogeometric acoustic model with ground reflection. The Helmholtz equation for the acoustic problem is solved by the boundary element method (BEM), and the model structure shape is optimized by combining the isogeometric method. In addition, to mitigate the high computational cost arising from repeated evaluations at each discrete frequency point, the Hankel function is approximated via a Taylor series expansion. This approach enables the decoupling of the boundary element method equation into frequency-dependent and frequency-independent terms. After using the deep neural network (DNN) training simulation results, the acoustic results are predicted. The DNN model can effectively analyze the sound field problem. Finally, the accuracy and feasibility of the proposed algorithm are verified by a two-dimensional numerical example.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251357783"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12304598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144735618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cochlear implantation in children with Bartter syndrome: A case report.","authors":"Khalid M Badr, Syed A Raza, Asma Alahmadi, Fares Alghamdi, Alsaeid M Thabet, Saeed Alghamdi","doi":"10.1177/00368504251349390","DOIUrl":"10.1177/00368504251349390","url":null,"abstract":"<p><p>Bartter syndrome (BS) is an autosomal recessive disorder characterized by polyhydramnios, premature birth, polyuria, renal salt-wasting, hypokalemic metabolic alkalosis, normal blood pressure with increased levels of renin and aldosterone, and the presence of hearing loss. Mutations in BSND, CLCNKA, and CLCNKB cause the disorder. We present a 3-year-old girl with BS type 4 and sensorineural hearing loss who received cochlear implantation (CI) after failing a hearing screening. The patient had a successful left-side implantation but later developed a flap infection, initially treated with antibiotics and surgical repair using a vascularized flap, which failed. During the same repair procedure, the right ear was implanted successfully. A third surgery was required to remove the left implant and close the wound with a temporoparietal flap, performed by plastic surgery. This is the earliest report on managing device extrusion in BS. The literature review highlights that the outcomes of CI in patients with BS vary significantly within each syndrome group and that the presence of additional disabilities accentuates this diversity. Our case illustrates that CI can improve the quality of life for patients with BS, but a multidisciplinary team is crucial for managing these cases. We stress the need for further studies to evaluate realistic outcomes and complication rates, as this will guide future treatment strategies.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251349390"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative lipid levels and prognosis in non-small cell lung cancer: A retrospective age-stratified study.","authors":"Yv-Xuan Liu, Jing Chen, Chen Liu, Maierhaba Maitiyasen, Xinyv Hou, Jingfeng Li, Jun Yi","doi":"10.1177/00368504251352375","DOIUrl":"10.1177/00368504251352375","url":null,"abstract":"<p><p>BackgroundAlthough abnormalities in circulating lipids and lipoproteins are associated with increased cancer risk, their specific impact on lung cancer progression and prognosis is still unclear. This study retrospectively assessed the influence of preoperative lipid and lipoprotein levels on non-small cell lung cancer progression and prognosis, stratified by age.MethodsIn this retrospective study, we analyzed 849 patients to investigate the association between lipid markers and lung cancer progression, and examined postoperative prognosis in a subset of 222 patients. Data was analyzed using restricted cubic spline curves, Kaplan-Meier survival analysis, and Cox proportional hazards models.ResultsA significant nonlinear relationship was observed between total cholesterol (TC), high-density lipoprotein (HDL), ApoB, ApoAI, ApoE, and baseline tumor diameter (BSLD) (PTC = 0.025; PHDL < 0.001; PApoB = 0.037; PApoAI =0.001; PApoE < 0.001). In contrast, Lp(a) showed a significant linear relationship with BSLD (P = 0.002). The Cox regression analysis revealed that triglyceride (TG) (hazard ratio (HR) = 0.50, 95% confidence interval (CI): 0.28-0.92, P = 0.025) was significantly negatively associated with lung cancer mortality in patients under 58 years. For patients over 58 years, higher ApoB levels were linked to a reduced risk of lung cancer death (HR = 0.59, 95% CI: 0.36-0.97, P = 0.038).ConclusionThis study reveals a significant negative correlation between ApoAI and HDL levels with BSLD, while Lp(a) shows a positive correlation. In terms of long-term prognosis, high-serum ApoB are associated with a lower mortality risk in all lung cancer patients, and high-serum TG levels associated with reduced mortality risk in patients aged under 58 while high-serum TC levels associated with reduced mortality risk in patients over 58, with high Lp(a) levels indicating a greater risk of mortality in older patients.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251352375"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive model of double-J pipe scab size after radical total cystectomy based on Boruta feature selection and LASSO technique: A retrospective cross-sectional study.","authors":"Cheng Shen, Haishun Ni, Zhan Chen, Junjie You, Bing Zheng","doi":"10.1177/00368504251363901","DOIUrl":"https://doi.org/10.1177/00368504251363901","url":null,"abstract":"<p><p>ObjectiveThis study sought to develop a prediction model for double-J pipe scab following radical total cystectomy.MethodsThis retrospective study included clinical data on 175 patients who underwent routine double-J pipe replacement after radical total cystectomy. A double-J pipe scab was the nomogram's outcome. Boruta feature selection and the least absolute shrinkage selection operator (LASSO) approach were employed to predictions as efficiently as possible. Using multiple logistic regression, predictive models were created and displayed as nomograms. Nomogram performance was assessed using decision curve analysis, calibration plots, and receiver operating characteristic (ROC) curves. By computing the validation cohort's performance, the model was internally validated.ResultsThis study covered 175 patients in total. In twenty-nine individuals (16.57%), a double-J pipe scab formed. Every participant was randomly split into two groups: training (<i>n</i> = 122) and validation (<i>n</i> = 53). As predictors, this nomogram included urine leukocytes, urinary PH, daily water intake, BMI, and double-J pipe brand. Excellent identification performance is indicated by the training and verification groups' ROC curves, while the calibration curves demonstrate both groups' excellent correction outcomes.ConclusionThis study establishes a foundation for preventing and treating double-J pipe stones. It has a substantial predictive value for the occurrence of double-J pipe stones in the double-J pipe following radical total cystectomy.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251363901"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144746130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A stage IV high-grade serous ovarian cancer patient carrying <i>ERBB2</i> amplification and ERBB2 overexpression benefits from late-line pyrotinib treatment: A case report.","authors":"Baorang Zhu, Ying Liu, Jing Li, Wuwei Yang","doi":"10.1177/00368504251362310","DOIUrl":"10.1177/00368504251362310","url":null,"abstract":"<p><p>Ovarian cancer (OC), one of the most common gynecological cancers, is usually diagnosed at an advanced stage. High-grade serous ovarian cancer (HGSOC) is a histological subtype of OC that accounts for approximately 70% of all OC cases. erb-b2 receptor tyrosine kinase 2 (ERBB2) overexpression commonly occurs in HGSOC, accounting for 12.5% of stage III and IV HGSOCs. Currently, there is no established treatment strategy for OC patients with <i>ERBB2</i> amplification or ERBB2 overexpression. Whether these patients will benefit from ERBB2 inhibitors remains unclear. Herein, we report a patient with metastatic HGSOC carrying <i>ERBB2</i> amplification and ERBB2 overexpression. Following progression to platinum-based chemotherapy and subsequent antiangiogenic therapies, the patient benefited from late-line pyrotinib treatment, achieving stable disease with a progression-free survival of 9.5 months. Our study provides preliminary clinical evidence supporting pyrotinib as a feasible treatment option for metastatic HGSOC patients with <i>ERBB2</i> amplification and/or ERBB2 overexpression. Prospective trials of pyrotinib should be conducted in appropriately selected OC populations.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251362310"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balloon-assisted ExoSeal closure for inadvertent subclavian artery cannulation following central venous catheterization: A case report.","authors":"Yu Wang, Tao Liu, Xuemei Zhou, Fa-Cai Guo","doi":"10.1177/00368504251363121","DOIUrl":"https://doi.org/10.1177/00368504251363121","url":null,"abstract":"<p><p>Accidental arterial puncture is a rare but significant complication associated with central venous catheterization. This report describes a case of inadvertent subclavian artery catheterization that was successfully managed with the ExoSeal vascular closure device. A female patient in her early 30s was hospitalized for severe acute pancreatitis that necessitated cannulation of the right subclavian vein. Postprocedure, infusion difficulties suggested a need for arterial catheterization, which was confirmed by imaging. The intervention resulted in prompt hemostasis and prevented further vascular injury, resulting in complication-free recovery. This case highlights the importance of rapid identification and treatment of arterial injury during central venous catheterization, and also demonstrates the effectiveness of ExoSeal in the management of vascular complications, especially in high-risk conditions.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251363121"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of peach seedlings (<i>Prunus davidiana</i>) experiencing drought stress.","authors":"Ruijin Zhou, Baoquan Wang, Qianwen Liu, Shuda Li, Lulu Zhang","doi":"10.1177/00368504251358640","DOIUrl":"https://doi.org/10.1177/00368504251358640","url":null,"abstract":"<p><p>The frequency of drought is expected to rise in many parts of the world with increasing climate change. Despite being an economically valuable plant species, the molecular mechanisms regulating the responses of peach (<i>Prunus davidiana</i>) to drought stress and the functional genes conferring drought resistance are currently unknown. In this study, we investigated the phenotypic and physiological responses of peach seedlings to experimental conditions that included a control, a period of drought stress, and a rehydration period. We performed transcriptome sequencing and investigated differences in the transcriptome of peach seedlings exposed to different treatments. We also analyzed the functions and regulatory pathways of differentially expressed genes using GO and KEGG enrichment. The results showed that severe drought stress occurred in the peach seedlings on the sixth day of drought, and that the physiological responses of peach seedlings experiencing drought stress were significantly different from those in control conditions. We found 21348 differentially expressed genes in peach seedlings under drought stress, of which 10105 were up-regulated and 11243 were down-regulated in comparison with peach seedlings in control conditions. These differentially expressed genes were mainly involved in the biosynthesis of amino acids, metabolic pathways, antioxidant defense systems and the plant hormone signal transduction system. The results suggest that peach seedlings respond to severe drought stress by initiating antioxidant defense mechanisms to alleviate damages, activating different signal transduction pathways to transmit signals, regulating the synthesis of amino acids, and initiating metabolic mechanisms to enhance osmotic pressure. This study illuminates the mechanisms for drought resistance in peach seedlings at the molecular level. Overall, the findings provide a theoretical basis for the cloning and functional analysis of genes conferring drought resistance, and the cultivation of more drought resistant varieties of peach.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251358640"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic findings as a prognostic factor in parotid cancer.","authors":"Jeong Heon Kim, Jeong Ho Woo, Minsu Kwon, Young Ho Jung, Seung-Ho Choi, Yoon Se Lee","doi":"10.1177/00368504251356179","DOIUrl":"10.1177/00368504251356179","url":null,"abstract":"<p><p>ObjectiveRecent advancements in high-resolution ultrasonography (US) have established it as a critical tool for evaluating parotid lesions. However, the lack of standardized diagnostic criteria limits the utility of US in determining malignancy. This study investigates the potential role of US as a prognostic factor in parotid cancer.MethodsPatients diagnosed with and surgically treated for parotid cancer at our tertiary referral center from January 2016 to December 2022 were included in this retrospective cohort study. We retrospectively obtained patient data including US images and clinical factors and analyzed their correlation with various adverse features and oncological outcomes, including five-year disease-free survival (5Y DFS) and overall survival (5Y OS).ResultsA total of 126 patients were included. The 5Y DFS and 5Y OS were 81.7% ± 3.7% and 81.2% ± 4.1% respectively. Multivariate analysis revealed that age (DFS; HR 2.75 [1.76-4.29], <i>p</i> = .023, OS; HR 3.38 [2.06-5.54], <i>p</i> = .014), clinical nodal stage (DFS; HR 5.87 [3.74-9.21], <i>p</i> < .001, OS; HR 9.34 [5.48-15.91], <i>p</i> < .001) and the presence of posterior enhancement artifact on US (DFS; HR 0.33 [0.21-0.53], <i>p</i> = .019, OS; HR 0.34 [0.20-0.57], <i>p</i> = .037) were significant variables. In patients with early-stage parotid cancer who showed posterior enhancement, the extent of surgery did not affect treatment outcomes.ConclusionPosterior acoustic enhancement on ultrasonography is a favorable prognostic factor in parotid cancer. For patients with early-stage cancer who demonstrate posterior enhancement, minimizing the extent of surgery does not compromise oncologic outcomes.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251356179"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144531295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical management of giant neurofibroma in a pediatric patient: A case report.","authors":"Pidong Liu, Yanwei Sun","doi":"10.1177/00368504251357509","DOIUrl":"10.1177/00368504251357509","url":null,"abstract":"<p><p>Neurofibromas are benign peripheral nerve sheath tumors that occur in individuals with neurofibromatosis type 1. In pediatric patients, giant neurofibromas pose significant management challenges due to their large size and potential to cause functional impairments. Given their characteristic radiation resistance, indolent growth, and limited chemosensitivity, surgical intervention is frequently required to alleviate tumor-related symptoms, restore function, and mitigate complications. The two primary surgical difficulties are the high recurrence rates resulting from the infiltrative nature of giant neurofibromas and the risk of massive intraoperative hemorrhage. This case report details the surgical management of a pediatric giant neurofibroma, highlighting the multidisciplinary strategy and operative techniques employed to achieve optimal results.</p>","PeriodicalId":56061,"journal":{"name":"Science Progress","volume":"108 3","pages":"368504251357509"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}