Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology最新文献_第2页

Comparative mechanisms for O₂ storage and metabolism in two Florida diving birds: the anhinga (Anhinga anhinga) and the double-crested cormorant (Nannopterum auritum). 两种佛罗里达潜水鸟类：凤头鸟（anhinga anhinga）和双冠鸬鹚（Nannopterum auritum）的氧储存和代谢比较机制。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-04-01 Epub Date: 2024-12-20 DOI: 10.1007/s00360-024-01593-x

Jeff White, Elizabeth R Schell, Neal J Dawson, Kevin G McCracken

{"title":"Comparative mechanisms for O2 storage and metabolism in two Florida diving birds: the anhinga (Anhinga anhinga) and the double-crested cormorant (Nannopterum auritum).","authors":"Jeff White, Elizabeth R Schell, Neal J Dawson, Kevin G McCracken","doi":"10.1007/s00360-024-01593-x","DOIUrl":"10.1007/s00360-024-01593-x","url":null,"abstract":"Air-breathing vertebrates face many physiological challenges while breath-hold diving. In particular, they must endure intermittent periods of declining oxygen (O2) stores, as well as the need to rapidly replenish depleted O2 at the surface prior to their next dive. While many species show adaptive increases in the O2 storage capacity of the blood or muscles, others increase the oxidative capacity of the muscles through changes in mitochondrial arrangement, abundance, or remodeling of key metabolic pathways. Here, we assess the diving phenotypes of two sympatric diving birds: the anhinga (Anhinga anhinga) and the double-crested cormorant (Nannopterum auritum). In each, we measured blood- and muscle-O2 storage capacity, as well as phenotypic characteristics such as muscle fiber composition, capillarity, and mitochondrial arrangement and abundance in the primary flight (pectoralis) and swimming (gastrocnemius) muscles. Finally, we compared the maximal activities of 10 key enzymes in the pectoralis, gastrocnemius, and left ventricle of the heart to assess tissue level oxidative capacity and fuel use. Our results indicate that both species utilize enhanced muscle-O2 stores over blood-O2. This is most apparent in the large difference in available myoglobin in the gastrocnemius between the two species. Oxidative capacity varied significantly between the flight and swimming muscles and between the two species. However, both species showed lower oxidative capacity than expected compared to other diving birds. In particular, the anhinga exhibits a unique diving phenotype with a slightly higher reliance on glycolysis and lower aerobic ATP generation than double-crested cormorants.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"191-208"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of melatonin and its agonist on blood pressure and serum endothelin-1 in continuous light and pinealectomized rats. 褪黑素及其激动剂对连续光照和去松果体大鼠血压和血清内皮素-1的影响。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-04-01 Epub Date: 2025-03-14 DOI: 10.1007/s00360-025-01610-7

Evan B Othman, Ismail M Maulood, Nazar M Shareef Mahmood

{"title":"The impact of melatonin and its agonist on blood pressure and serum endothelin-1 in continuous light and pinealectomized rats.","authors":"Evan B Othman, Ismail M Maulood, Nazar M Shareef Mahmood","doi":"10.1007/s00360-025-01610-7","DOIUrl":"10.1007/s00360-025-01610-7","url":null,"abstract":"The present study investigates the roles of melatonin (MEL) and its agonist ramelteon (RAM) on blood pressure regulation, nitric oxide (NO), and oxidative stress and plasma endothelin-1(ET-1) levels in continuous light exposure and pinealectomized conditions. This study includes two experiments. The first experiment involved control, continuous light emitting diode (LED) exposure, continuous LED + MEL administration, and continuous LED + RAM. The second experiment included control, pinealectomy, pinealectomy + MEL administration, pinealectomy + RAM administration, and pinealectomy + continuous LED exposure. The present results showed significant increase of systolic blood pressure (SBP) of continuous LED exposure group, pinealectomy, and pinealectomy with continuous LED exposure. On the contrary, MEL and RAM both decreased SBP. Additionally, the continuous LED exposure considerably increased malondialdehyde (MDA). However, MEL increased both plasma ET-1 slightly and ET-1 significantly but RAM dramatically increased ET-1. While, both of MEL and RAM decreased MDA. In the second experiment, while MDA dramatically increased after pinealectomy and pinealectomy with LED illumination, ET-1 and NO were only a little reduced. Melatonin elevated plasma ET-1 and NO significantly. While, MDA was greatly reduced by MEL but not by RAM. The results suggested that MEL and RAM could attenuate SBP mostly via increasing NO generation and oxidative stress reduction.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"215-225"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophysiological evidence for the right atrioventricular pacemaker ring in the reptile heart. 爬行动物心脏右房室起搏环的电生理学证据。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-04-01 Epub Date: 2025-03-28 DOI: 10.1007/s00360-025-01612-5

Valentina Prosheva, Vladimir Vityazev, Elena Lebedeva

{"title":"Electrophysiological evidence for the right atrioventricular pacemaker ring in the reptile heart.","authors":"Valentina Prosheva, Vladimir Vityazev, Elena Lebedeva","doi":"10.1007/s00360-025-01612-5","DOIUrl":"10.1007/s00360-025-01612-5","url":null,"abstract":"Pacemaker atrioventricular (AV) rings, continuous with the AV node, have been shown to be present in the mammalian and avian hearts. There is conspicuous lack of electrophysiological data on the cardiac pacemakers in reptiles. We aimed to characterize the AV ring in the common lizard heart for the first time using conventional microelectrode technique. Detaching the sinoatrial (SA) area unmasked pacemaking in the AV junction. In all seven studied isolated AV ring preparations, we could record action potentials (APs) with characteristic diastolic depolarization, with a slow upstroke (dV/dt max) of 3.5 ± 0.3 V s-1 and a low amplitude of 57.8 ± 1.3 mV. The cells with pacemaking potentiality were found to surround the atrial orifice of the right AV valve. We identified some commonalities between phenotype of right AV ring pacemaker APs and SA dominant pacemaker ones. Thus, the AV ring in the reptile heart demonstrates pacemaking activity and contains cells that resemble the electrophysiological characteristics of mammalian and avian pacemaker myocytes in AV rings surrounded the atrial orifices of AV valves.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"181-190"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic insights into the low-salinity tolerance of the sea louse Caligus elongatus. 海虱耐低盐度转录组学研究。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-04-01 Epub Date: 2025-02-28 DOI: 10.1007/s00360-025-01606-3

Andreas Borchel, Frank Nilsen

{"title":"Transcriptomic insights into the low-salinity tolerance of the sea louse Caligus elongatus.","authors":"Andreas Borchel, Frank Nilsen","doi":"10.1007/s00360-025-01606-3","DOIUrl":"10.1007/s00360-025-01606-3","url":null,"abstract":"Caligus elongatus is a marine copepod ectoparasite on a wide variety of fish species. It has also been observed on fish farms cultivating Atlantic salmon and reports shows that this parasite can be a problem for the industry and for the fish's welfare. Freshwater is used as one of the non-medical treatment methods against the salmon louse (Lepeophtheirus salmonis). However, the efficacy of freshwater treatment against C. elongatus is still unknown. This study aims to fill this gap by examining the salinity tolerance limits of both adult and copepodid life stages of C. elongatus. Our findings reveal that detached adult C. elongatus exhibit low tolerance to reduced salinity, with mortality occurring within hours at salinities below 20 ppt. In contrast, copepodid stages demonstrated a slightly higher tolerance, surviving at salinities as low as 15 ppt for one day. Adult lice attached to a host quickly detached from the fish as soon as the salinity was lower than 20 ppt, suggesting that freshwater delousing might be effective in this species. To further understand the genetic basis of acclimation to reduced salinities, we performed RNA-sequencing to assemble the first transcriptome of this species and identify differentially expressed genes. Several genes regulated upon low-salinity transfer were identified. These include genes involved in proline metabolism, energy metabolism, and the transport of various ions and betaine, an osmolyte. The potential roles of these genes in salinity acclimation are discussed within an evolutionary context, providing valuable insights into the survival mechanisms of C. elongatus under low-salinity conditions.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"155-171"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of acute cooling and bradycardia on central venous pressure and cardiac function in Nile tilapia (Oreochromis niloticus). 急性降温和心动过缓对尼罗罗非鱼中心静脉压和心功能的影响。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-04-01 Epub Date: 2025-01-07 DOI: 10.1007/s00360-024-01600-1

E S Porter, A K Gamperl

{"title":"Effects of acute cooling and bradycardia on central venous pressure and cardiac function in Nile tilapia (Oreochromis niloticus).","authors":"E S Porter, A K Gamperl","doi":"10.1007/s00360-024-01600-1","DOIUrl":"10.1007/s00360-024-01600-1","url":null,"abstract":"We developed and validated a surgical technique to measure central venous pressure (CVP) in Nile tilapia, and investigated the effects of an acute temperature decrease (from 30 vs. 24 °C) and changes in heart rate (fH) using zatebradine hydrocholoride, which decreases intrinsic fH, on this species' cardiac function. As predicted, fH and cardiac output ( <math><mover><mi>Q</mi> <mo>˙</mo></mover> </math> ) were ~ 40% lower in the acutely cooled fish, and both groups had very comparable (i.e., within 10%) values for stroke volume (VS). The CVP of fish acutely exposed to 24 °C was consistently ~ 0.04 kPa higher than in those measured at 30 °C across all concentrations of zatebradine (i.e., CVP increased from 0.04 to 0.11 kPa vs. - 0.01-0.07 kPa for 24 vs. 30 °C tilapia, respectively, as fH was reduced). However, this did not result in an increase in VS due to a right-shifted relationship between CVP and VS for the 24 °C fish. These data suggest that the VS of tilapia is less sensitive to changes/increases in CVP when temperature is acutely lowered, and that regardless of increases in preload (CVP), <math><mover><mi>Q</mi> <mo>˙</mo></mover> </math> is primarily modulated by fH in this species.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"173-179"},"PeriodicalIF":1.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digestive and metabolic profile of the resident population of the silverside Odontesthes argentinensis from Mar Chiquita Coastal Lagoon (Buenos Aires, Argentina). 阿根廷布宜诺斯艾利斯Mar Chiquita海岸泻湖居住的阿根廷银鱼的消化和代谢特征。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-02-01 Epub Date: 2025-01-20 DOI: 10.1007/s00360-024-01594-w

Albanesi Camila, Méndez Eugenia, González-Castro Mariano, López-Mañanes Alejandra, Michiels María Soledad

{"title":"Digestive and metabolic profile of the resident population of the silverside Odontesthes argentinensis from Mar Chiquita Coastal Lagoon (Buenos Aires, Argentina).","authors":"Albanesi Camila, Méndez Eugenia, González-Castro Mariano, López-Mañanes Alejandra, Michiels María Soledad","doi":"10.1007/s00360-024-01594-w","DOIUrl":"10.1007/s00360-024-01594-w","url":null,"abstract":"The knowledge about the occurrence and biochemical characteristics of key digestive enzymes is crucial for an enhanced understanding of the dietary ecophysiology of the species. On the other hand, integrative studies on digestive physiology and on tissue content of glycogen, glucose, lipid and protein in groups of ecological and economic importance are currently limited. In this work, we determined the occurrence and biochemical characteristics in intestine of key digestive enzymes activities as indexes of the ability to digest different dietary substrates and of functional differentiation for digestion/absorption of nutrients along with the intestinal coefficient as index of dietary habit and digestion efficiency in adults of Odonthtestes argentinensis inhabiting Mar Chiquita Coastal Lagoon (Buenos Aires, Argentina). Furthermore, to identify storage sites, glycogen, triglycerides and protein content in different tissues were also analyzed. The presence and biochemical characteristics of amylase, maltase, sucrase, lipase, trypsin and aminopeptidase-N activity in intestine, as well as the tissue content of glycogen, triglycerides and protein suggests that adults of O.argentinensis exhibit an adequate digestive battery to potentially perform complete hydrolysis of various dietary substrates and capacity for storage and/or utilization of energy reserves. Our study provides novel insights into the digestive/metabolic traits in adults of the resident silverside O. argentinensis from Mar Chiquita Coastal Lagoon.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"31-37"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In renal proximal tubular epithelial cells of the hibernator Syrian hamster, anoxia-reoxygenation-induced reactive oxygen species bursts do not trigger a DNA damage response and cellular senescence. 在冬眠的叙利亚仓鼠肾近曲小管上皮细胞中，缺氧-复氧诱导的活性氧爆发不会引发 DNA 损伤反应和细胞衰老。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI: 10.1007/s00360-025-01604-5

Georgios Pissas, Maria Divani, Maria Tziastoudi, Christina Poulianiti, Maria-Anna Polyzou-Konsta, Evangelos Lykotsetas, Ioannis Stefanidis, Theodoros Eleftheriadis

{"title":"In renal proximal tubular epithelial cells of the hibernator Syrian hamster, anoxia-reoxygenation-induced reactive oxygen species bursts do not trigger a DNA damage response and cellular senescence.","authors":"Georgios Pissas, Maria Divani, Maria Tziastoudi, Christina Poulianiti, Maria-Anna Polyzou-Konsta, Evangelos Lykotsetas, Ioannis Stefanidis, Theodoros Eleftheriadis","doi":"10.1007/s00360-025-01604-5","DOIUrl":"10.1007/s00360-025-01604-5","url":null,"abstract":"Ischemia-reperfusion (I-R) injury represents a predominant etiology of acute kidney injury (AKI), for which effective treatments remain unavailable. In contrast, hibernating mammals exhibit notable resistance to cell death induced by I-R injury. However, the impact of I-R injury on cellular senescence-an important factor in AKI-has not been extensively studied in these species. Comparative biology may offer novel therapeutic insights. Renal proximal tubular epithelial cells (RPTECs) from the native hibernator Syrian hamster or mouse RPTECs were subjected to anoxia-reoxygenation. Proteins involved in DNA damage response (DDR) and cellular senescence were assessed using western blotting, reactive oxygen species (ROS) levels and cell death were quantified colorimetrically, and IL-6 with ELISA. Anoxia-reoxygenation induced oxidative stress in both mouse and hamster RPTECs; however, cell death was observed exclusively in mouse cells. While anoxia-reoxygenation elicited a DDR and subsequent senescence in mouse RPTECs, such responses were not detected in hamster RPTECs. Thus, RPTECs from the Syrian hamster exhibited increased ROS production upon reoxygenation but did not show DDR or cellular senescence. Further research is required to elucidate the specific protective molecular mechanisms in hibernators, which could potentially lead to the development of novel therapeutic approaches for I-R injury in non-hibernating species, including humans.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"91-101"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The flavouring agent, 2-octenoic acid kills Galleria mellonella larvae by affecting the cellular and humoral elements of insect immunological system. 风味剂2-辛烯酸通过影响昆虫免疫系统的细胞和体液成分来杀死大黄蜂幼虫。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI: 10.1007/s00360-025-01603-6

Agata Kaczmarek, Mieczysława Irena Boguś

{"title":"The flavouring agent, 2-octenoic acid kills Galleria mellonella larvae by affecting the cellular and humoral elements of insect immunological system.","authors":"Agata Kaczmarek, Mieczysława Irena Boguś","doi":"10.1007/s00360-025-01603-6","DOIUrl":"10.1007/s00360-025-01603-6","url":null,"abstract":"Larvae of Galleria mellonella are well known for their parasitisation of honeybees, so developing new methods of controlling the pest population is an important issue. The present research examined the immunotoxic effects of 2-octenoic acid against wax moth larvae. The last instar larvae were used for all experimental analyses. The tested fatty acid doses LD50 and LD100 (9.66 µg/mg and 11.72 µg/mg of body mass) were applied topically to insects under in vivo conditions and the hemolymph was collected after 24 and 48 h. To check the in vitro impact of the 2-octenoic acid, the examined fatty acid was given directly to the cultured hemocytes (to a final concentration: 0.33 and 0.16 µg/µl) and incubated for 24 and 48 h. Current research using fluorescence microscopy and spectrofluorimetric measurements indicates the death of immunocompetent cells via the apoptosis pathway. Moreover, it shows the activation of caspases and an increase in the level of reactive oxygen/nitrogen damage after both in vivo and in vitro treatment of 2-octenoic acid. This points to the impact of both cellular and humoral elements on the immunological response to the toxic compound. Hence, 2-octenoic acid seems to have significant potential as an insecticide while being safe for humans and the environment. Therefore, further research into its potential is warranted.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"13-22"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is the air-breathing organ a significant route for CO₂ excretion during aquatic hypercapnia in the pirarucu, Arapaima gigas? 在水高碳酸血症期间，呼吸器官是大腹鱼二氧化碳排泄的重要途径吗？

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-02-01 Epub Date: 2024-12-20 DOI: 10.1007/s00360-024-01597-7

Chris M Wood, Bernd Pelster, Adalberto Luis Val

{"title":"Is the air-breathing organ a significant route for CO2 excretion during aquatic hypercapnia in the pirarucu, Arapaima gigas?","authors":"Chris M Wood, Bernd Pelster, Adalberto Luis Val","doi":"10.1007/s00360-024-01597-7","DOIUrl":"10.1007/s00360-024-01597-7","url":null,"abstract":"The pirarucu is one of the very few obligate air-breathing fish, employing a gigantic, highly vascularized air-breathing organ (ABO). Traditionally, the ABO is thought to serve mainly for O2 uptake (ṀO2), with the gills providing the major route for excretion of CO2 (ṀCO2) and N-waste. However, under aquatic hypercapnia, a common occurrence in its natural environment, branchial ṀCO2 to the water may become impaired. Under these conditions, does the ABO become an important route of ṀCO2 excretion to the air? We have answered this question by measuring ṀCO2 and ṀO2 in both air and water phases, as well as the pattern of air-breathing, in pirarucu under aquatic normocapnia and hypercapnia (3% CO2). Indeed, ṀCO2 to the air phase via the ABO increased 2- to 3-fold during exposure to high water PCO2, accounting for 59-71% of the total, with no change in the dominant contribution of the ABO to ṀO2 (71-75% of the total). These adjustments were quickly reversed upon restoration of aquatic normocapnia. During aquatic hypercapnia, ṀCO2 via the ABO became more effective over time, and the pattern of air-breathing changed, exhibiting increased frequency and decreased breath volume. Ammonia-N excretion (86-88% of total) dominated over urea-N excretion and tended to increase during exposure to aquatic hypercapnia. We conclude that the ability of the ABO to take on the dominant role in CO2 excretion when required may have been an important driver in the original evolution of air-breathing, as well as in the functionality of the ABO in modern air-breathing fish.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"39-51"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sleep deprivation stimulates adaptive thermogenesis by activating AMPK pathway in mice. 剥夺睡眠通过激活 AMPK 途径刺激小鼠的适应性产热。

IF 1.7 3区生物学

Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology Pub Date : 2025-02-01 Epub Date: 2024-10-30 DOI: 10.1007/s00360-024-01590-0

Tian-Shu Zheng, Xin-Ran Gao, Rui-Ping Xu, Yi-Fei Zhao, Zhi-Teng Yang, De-Hua Wang

{"title":"Sleep deprivation stimulates adaptive thermogenesis by activating AMPK pathway in mice.","authors":"Tian-Shu Zheng, Xin-Ran Gao, Rui-Ping Xu, Yi-Fei Zhao, Zhi-Teng Yang, De-Hua Wang","doi":"10.1007/s00360-024-01590-0","DOIUrl":"10.1007/s00360-024-01590-0","url":null,"abstract":"Sleep deprivation (SD) can affect the adaptive thermogenesis in laboratory rodents, but the molecular mechanism and the crosstalk with other organs remain largely unknown. In order to investigate the effects and mechanisms of SD on thermoregulation and energy metabolism, here we measured the changes of body weight, body fat mass, body temperature, resting metabolic rate (RMR), and thermogenic gene expression in brown adipose tissue (BAT), white adipose tissue (WAT), skeleton muscle and liver in C57BL/6J mice during 7-day SD with rotating rod sleep deprivation device. Results showed that compared with the control group, the body weight and body fat mass of SD mice were decreased and RMR of SD mice increased. The gene expression of Ampk, Pgc1α and Ucp1 which related to thermogenesis in BAT and WAT were significantly increased, and the expression of Ampk, Serca1, Serca2 and Ucp3 which related to thermogenesis in skeletal muscle were significantly increased in SD mice. Taken together, these data demonstrated that 7-day SD enhanced the adaptive thermogenesis in mice by activating AMPK, including the upregulation of the AMPK - PGC1α - UCP1 pathway in BAT, and the AMPK - UCP3 and SLN - SERCA pathway in skeleton muscle. Our data provide the molecular evidence for SD-stimulated adaptive thermogenesis and energy metabolism in small mammals.","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"141-153"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0