Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology最新文献

{"title":"Differential fatty acids utilization across life stages in a Vespa species.","authors":"Sofia Bouchebti, Eran Levin","doi":"10.1007/s00360-024-01589-7","DOIUrl":"10.1007/s00360-024-01589-7","url":null,"abstract":"<p><p>Dietary fatty acids (FAs) are essential macronutrients affecting animal fitness, growth, and development. While the degree of saturation of FAs usually determines the level of absorption and allocation within the body, the utilization of dietary FAs across the life stages of individuals remains unknown. We used three different 13 C labeled FAs, with a different saturation level (linoleic acid (18:2), oleic acid (18:1), and palmitic acid (16:0)), to investigate the absorption and allocation of dietary FAs across the life stages of the Oriental hornet. Our results show that only larvae utilized all tested FAs as metabolic fuel, with palmitic acid being oxidized at the highest rate. Oleic and palmitic acids were predominantly incorporated into larval tissues, while oleic acid dominated pupal tissues. In contrast, linoleic and oleic acids were predominantly incorporated into adult tissues. These findings highlight a life stage-dependent shift in certain FAs utilization, with palmitic acid mostly utilized in early stages and linoleic acid in adulthood, while oleic acid remained consistently utilized across all life stages. This study emphasizes the importance of considering FA saturation and life stage dynamics in understanding FA utilization patterns.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"23-29"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apneic uptake of atmospheric O₂ by deeply hypothermic nestlings of the white-footed mouse (Peromyscus leucopus): circulation and lungs.","authors":"Richard W Hill, Jacob J Manteuffel, Bradley A White","doi":"10.1007/s00360-024-01585-x","DOIUrl":"10.1007/s00360-024-01585-x","url":null,"abstract":"<p><p>Nestling white-footed mice (Peromyscus leucopus) are born in the earliest days of spring in cold climates. If the nestlings are by accident exposed to ambient temperatures near freezing (0-7 °C) at early ages (2-10 days old), they may experience body temperatures (T_bs) equally low. During such hypothermia, although their heart keeps beating, they become apneic (cease inhaling and exhaling). However, they have an exceptional ability (e.g., compared to Mus musculus) to tolerate these conditions for at least several hours, after which they revive if rewarmed by parents. This paper addresses the physiology of the apneic period. We show that apneic, hypothermic nestlings undergo physiologically important exchanges of gases with the atmosphere. These gas exchanges do not occur across the skin. Instead they occur via the trachea and lungs even though the animals are apneic. Most significantly, when hypothermic neonates are in apnea in ordinary air, they take up O₂ steadily from the atmosphere throughout the apneic period, and the evidence available indicates that this O₂ uptake is essential for the nestlings' survival. At T_bs of 2-7 °C, the nestlings' rate of O₂ consumption varies quasi-exponentially with T_b and averages 0.04 mL O₂ g^- 1 h^- 1, closely similar to the rate expressed by adult mammalian hibernators in hibernation at similar T_bs. Morphometric analysis indicates that, at all focal ages, O₂ transport along the full length of the trachea can take place by diffusion at rates adequate to meet the measured rates of metabolic O₂ consumption.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"123-139"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of intestinal amino acid and oleic acid absorption and their interaction in the Pacific spiny dogfish (Squalus suckleyi).","authors":"Alyssa M Weinrauch, Tamzin A Blewett, W Gary Anderson","doi":"10.1007/s00360-024-01601-0","DOIUrl":"10.1007/s00360-024-01601-0","url":null,"abstract":"<p><p>Elasmobranchs are commonly carnivores and are important in energy transfer across marine ecosystems. Despite this, relatively few studies have examined the physiological underpinnings of nutrient acquisition in these animals. Here, we investigated the mechanisms of uptake at the spiral valve intestine for two representative amino acids (_L-alanine, _L-leucine) and one representative fatty acid (oleic acid), each common to the diet of a carnivore, the Pacific spiny dogfish (Squalus suckleyi). Transport was saturable for all three nutrients, depending upon transport calculation metric (i.e., mucosal disappearance, serosal appearance, or tissue accumulation). Over 0-10 mM range of amino acids the concentration at which ½ maximal transport occurred (K_m; a measure of transporter affinity) was 11.9 and 11.2 mM for tissue accumulation of alanine and leucine, respectively. Oleic acid transport was measured at lower concentrations (0-200 µM) and tissue accumulation did not reach saturation. Putative amino acid transport systems were delineated upon confirmation of sodium dependence and competitive inhibition with threonine, glycine, and lysine. The interplay of nutrient combinations on the modulation of nutrient acquisition rates, which better mimics the complex composition of both a meal and the internal osmolytes, was next investigated. Here, the application of serosal oleic acid led to diminished mucosal disappearance of leucine. Feeding did not significantly alter transport rates, perhaps indicative of maximal transport of these energy sources whenever the substrate is available given their importance both as metabolic fuels and precursors to the osmolyte urea.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"53-65"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acute cooling and bradycardia on central venous pressure and cardiac function in Nile tilapia (Oreochromis niloticus).","authors":"E S Porter, A K Gamperl","doi":"10.1007/s00360-024-01600-1","DOIUrl":"https://doi.org/10.1007/s00360-024-01600-1","url":null,"abstract":"<p><p>We developed and validated a surgical technique to measure central venous pressure (CVP) in Nile tilapia, and investigated the effects of an acute temperature decrease (from 30 vs. 24 °C) and changes in heart rate (f_H) using zatebradine hydrocholoride, which decreases intrinsic f_H, on this species' cardiac function. As predicted, f_H and cardiac output ( <math><mover><mi>Q</mi> <mo>˙</mo></mover> </math> ) were ~ 40% lower in the acutely cooled fish, and both groups had very comparable (i.e., within 10%) values for stroke volume (V_S)_. The CVP of fish acutely exposed to 24 °C was consistently ~ 0.04 kPa higher than in those measured at 30 °C across all concentrations of zatebradine (i.e., CVP increased from 0.04 to 0.11 kPa vs. - 0.01-0.07 kPa for 24 vs. 30 °C tilapia, respectively, as f_H was reduced). However, this did not result in an increase in V_S due to a right-shifted relationship between CVP and V_S for the 24 °C fish. These data suggest that the V_S of tilapia is less sensitive to changes/increases in CVP when temperature is acutely lowered, and that regardless of increases in preload (CVP), <math><mover><mi>Q</mi> <mo>˙</mo></mover> </math> is primarily modulated by f_H in this species.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative mechanisms for O₂ storage and metabolism in two Florida diving birds: the anhinga (Anhinga anhinga) and the double-crested cormorant (Nannopterum auritum).","authors":"Jeff White, Elizabeth R Schell, Neal J Dawson, Kevin G McCracken","doi":"10.1007/s00360-024-01593-x","DOIUrl":"https://doi.org/10.1007/s00360-024-01593-x","url":null,"abstract":"<p><p>Air-breathing vertebrates face many physiological challenges while breath-hold diving. In particular, they must endure intermittent periods of declining oxygen (O₂) stores, as well as the need to rapidly replenish depleted O₂ at the surface prior to their next dive. While many species show adaptive increases in the O₂ storage capacity of the blood or muscles, others increase the oxidative capacity of the muscles through changes in mitochondrial arrangement, abundance, or remodeling of key metabolic pathways. Here, we assess the diving phenotypes of two sympatric diving birds: the anhinga (Anhinga anhinga) and the double-crested cormorant (Nannopterum auritum). In each, we measured blood- and muscle-O₂ storage capacity, as well as phenotypic characteristics such as muscle fiber composition, capillarity, and mitochondrial arrangement and abundance in the primary flight (pectoralis) and swimming (gastrocnemius) muscles. Finally, we compared the maximal activities of 10 key enzymes in the pectoralis, gastrocnemius, and left ventricle of the heart to assess tissue level oxidative capacity and fuel use. Our results indicate that both species utilize enhanced muscle-O₂ stores over blood-O₂. This is most apparent in the large difference in available myoglobin in the gastrocnemius between the two species. Oxidative capacity varied significantly between the flight and swimming muscles and between the two species. However, both species showed lower oxidative capacity than expected compared to other diving birds. In particular, the anhinga exhibits a unique diving phenotype with a slightly higher reliance on glycolysis and lower aerobic ATP generation than double-crested cormorants.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whether hypoxia tolerance improved after short-term fasting is closely related to phylogeny but not to foraging mode in freshwater fish species.","authors":"Ke-Ren Huang, Qian-Ying Liu, Yong-Fei Zhang, Yu-Lian Luo, Cheng Fu, Xu Pang, Shi-Jian Fu","doi":"10.1007/s00360-024-01588-8","DOIUrl":"10.1007/s00360-024-01588-8","url":null,"abstract":"<p><p>The combined stresses of fasting and hypoxia are common events during the life history of freshwater fish species. Hypoxia tolerance is vital for survival in aquatic environments, which requires organisms to down-regulate their maintenance energetic expenditure while simultaneously preserving physiological features such as oxygen supply capacity under conditions of food deprivation. Generally, infrequent-feeding species who commonly experience food shortages might evolve more adaptive strategies to cope with food deprivation than frequent-feeding species. Thus, the present study aimed to test whether the response of hypoxia tolerance in fish to short-term fasting (2 weeks) varied with different foraging modes. Fasting resulted in similar decreases in maintenance energetic expenditure and similar decreases in P_crit and P_loe between fishes with different foraging modes, whereas it resulted in decreased oxygen supply capacity only in frequent-feeding fishes. Furthermore, independent of foraging mode, fasting decreased P_crit and P_loe in all Cypriniformes and Siluriformes species but not in Perciformes species. The mechanism for decreased P_crit and P_loe in Cypriniformes and Siluriformes species is at least partially due to the downregulated metabolic demand and/or the maintenance of a high oxygen supply capacity while fasting. The present study found that the effect of fasting on hypoxia tolerance depends upon phylogeny in freshwater fish species. The information acquired in the present study is highly valuable in aquaculture industries and can be used for species conservation in the field.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"843-853"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic effects of physical exercise on zebrafish (Danio rerio) fed a high-fat diet.","authors":"Moises Silvestre de Azevedo Martins, William Franco Carneiro, Kianne Silva Monteiro, Stefania Priscilla de Souza, André Rodrigues da Cunha Barreto Vianna, Luis David Solis Murgas","doi":"10.1007/s00360-024-01577-x","DOIUrl":"10.1007/s00360-024-01577-x","url":null,"abstract":"<p><p>The present study aimed to establish zebrafish as an experimental model for investigations into obesity and physical exercise, as well as to assess the effects of these factors on metabolism. The experiment spanned twelve weeks, comprising a feeding trial during which the last four weeks incorporated a physical exercise protocol. This protocol involved placing fifteen animals in a five-liter aquarium, where they were subjected to swimming at an approximate speed of 0.08 m/s for 30 min daily. Throughout the experiment, histological analyses of visceral, subcutaneous, and hepatic adipose tissues were conducted, along with biochemical analyses of total cholesterol and its fractions, triglycerides, glucose, lactate, and alanine aminotransferase (ALT) levels. Additionally, oxidative stress markers, such as reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and catalase activity and the formation of thiobarbituric acid-reactive substances, were investigated. The results revealed that the group fed a high-fat diet exhibited an increase in ROS production and SOD activity. In contrast, the group administered the high-fat diet and subjected to physical exercise demonstrated a notable reduction in visceral adipocyte area, hepatic steatosis levels, ALT levels, and SOD activity. These findings indicate that physical exercise has a positive effect on obesity and oxidative stress in zebrafish, providing promising evidence for future investigations in this field.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"793-804"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic rate and saliva cortisol concentrations in socially housed adolescent guinea pigs.","authors":"Matthias Nemeth, Susanna Fritscher, Klara Füreder, Bernard Wallner, Eva Millesi","doi":"10.1007/s00360-024-01576-y","DOIUrl":"10.1007/s00360-024-01576-y","url":null,"abstract":"<p><p>An individual's energetic demands and hence metabolic rate can strongly change during adolescence, a phase characterized by profound morphological, physiological, and endocrine changes. Glucocorticoid hormones (e.g. cortisol) are released in response to hypothalamic-pituitary-adrenal-axis activity, modulate several metabolic processes, and can also be linked to increased metabolic rate. In domestic guinea pigs (Cavia aperea f. porcellus) housed in same-sex groups, cortisol concentrations increase during adolescence in males but remain stable in females, which was suggested to be related to different energetic demands by age. We therefore measured metabolic rate through oxygen (O₂) consumption over 2.5 h in male and female guinea pigs housed in same-sex groups during adolescence at ages of 60, 120, and 180 days, which was paralleled by analyses of saliva cortisol concentrations before and after the measurement. The statistical analyses involved whole body metabolic rate (ml O₂/h), body mass-corrected metabolic rate (ml O₂/h/kg), and body mass-independent metabolic rate (ml O₂/h statistically corrected for body mass). We found increasing cortisol concentrations with age in males only, but none of the three metabolic rate analyses revealed a sex difference by age. On the individual level, repeatability across ages was found in metabolic rate as well as in body mass and cortisol concentrations after the measurement, but not in \"basal\" cortisol concentrations. Our results suggest no sex-specific changes in metabolic rate and hence equal energetic demands in male and female guinea pigs during adolescence. Moreover, metabolic rate clearly represents a highly stable physiological trait already early in a guinea pig's life irrespective of rather fluctuating cortisol concentrations.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"925-933"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress across multiple tissues in house sparrows (Passer domesticus) acclimated to warm, stable cold, and unpredictable cold thermal treatments.","authors":"Ana Gabriela Jiménez, Chelsi Marolf, David L Swanson","doi":"10.1007/s00360-024-01572-2","DOIUrl":"10.1007/s00360-024-01572-2","url":null,"abstract":"<p><p>With climate change increasing not just mean temperatures but the frequency of cold snaps and heat waves, animals occupying thermally variable areas may be faced with thermal conditions for which they are not prepared. Studies of physiological adaptations of temperate resident birds to such thermal variability are largely lacking in the literature. To address this gap, we acclimated winter-phenotype house sparrows (Passer domesticus) to stable warm, stable cold, and fluctuating cold temperatures. We then measured several metrics of the oxidative stress (OS) system, including enzymatic and non-enzymatic antioxidants and lipid oxidative damage, in brain (post-mitotic), kidney (mitotic), liver (mitotic) and pectoralis muscle (post-mitotic). We predicted that high metabolic flexibility could be linked to increases in reactive oxygen damage. Alternatively, if variation in ROS production is not associated with metabolic flexibility, then we predict no antioxidant compensation with thermal variation. Our data suggest that ROS production is not associated with metabolic flexibility, as we found no differences across thermal treatment groups. However, we did find differences across tissues. Brain catalase activity demonstrated the lowest values compared with kidney, liver and muscle. In contrast, brain glutathione peroxidase (GPx) activities were higher than those in kidney and liver. Muscle GPx activities were intermediate to brain and kidney/liver. Lipid peroxidation damage was lowest in the kidney and highest in muscle tissue.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"899-907"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of dissolved organic carbon and model compounds (DOC analogues) on diffusive water flux, oxygen consumption, nitrogenous waste excretion rates and gill transepithelial potential in Pacific sanddab (Citharichthys sordidus) at two salinities.","authors":"Carolyn Morris, Camila Martins, Samantha Zulian, D Scott Smith, Colin J Brauner, Chris M Wood","doi":"10.1007/s00360-024-01580-2","DOIUrl":"10.1007/s00360-024-01580-2","url":null,"abstract":"<p><p>Many flatfish species are partially euryhaline, such as the Pacific sanddab which spawn and feed in highly dynamic estuaries ranging from seawater to near freshwater. With the rapid increase in saltwater invasion of freshwater habitats, it is very likely that in these estuaries, flatfish will be exposed to increasing levels of dissolved organic carbon (DOC) of freshwater origin at a range of salinities. As salinity fluctuations often coincide with changes in DOC concentration, two natural freshwater DOCs [Luther Marsh (LM, allochthonous) and Lake Ontario (LO, autochthonous) were investigated at salinities of 30 and 7.5 ppt. Optical characterization of the two natural DOC sources indicate salinity-dependent differences in their physicochemistry. LO and LM DOCs, as well as three model compounds [tannic acid (TA), sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA)] representing key chemical moieties of DOC, were used to evaluate physiological effects on sanddabs. In the absence of added DOC, an acute decrease in salinity resulted in an increase in diffusive water flux (a proxy for transcellular water permeability), ammonia excretion and a change in TEP from positive (inside) to negative (inside). The effects of DOC (10 mg C L^-1) were salinity and source-dependent, with generally more pronounced effects at 30 than 7.5 ppt, and greater potency of LM relative to LO. Both LM DOC and SDS increased diffusive water flux at 30 ppt but only SDS had an effect at 7.5 ppt. TA decreased ammonia excretion at 7.5 ppt. LO DOC decreased urea-N excretion at both salinities whereas the stimulatory effect of BSA occurred only at 30 ppt. Likewise, the effects of LM DOC and BSA to reduce TEP were present at 30 ppt but not 7.5 ppt. None of the treatments affected oxygen consumption rates. Our results demonstrate that DOCs and salinity interact to alter key physiological processes in marine flatfish, reflecting changes in both gill function and the physicochemistry of DOCs between 30 and 7.5 ppt.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"805-825"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}