Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology最新文献

{"title":"Control of breathing during hibernation in golden-mantled ground squirrels.","authors":"Michael B Harris, William K Milsom","doi":"10.1007/s00360-024-01598-6","DOIUrl":"https://doi.org/10.1007/s00360-024-01598-6","url":null,"abstract":"<p><p>During hibernation, golden-mantled ground squirrels (Spermophilus lateralis) breathe in distinct multi-breath episodes separated by prolonged periods of apnea. We hypothesized that vagal afferent feedback from pulmonary stretch receptors, and N-methyl-D-aspartate (NMDA) type glutamate receptor-mediated processes in the pons are instrumental in the production of this breathing pattern and analyzed the effects of disrupting vagal feedback, and blockade of NMDA type receptors by the non-competitive antagonist (+)-5-methyl-10,11-dihydro-5 H-dibenzo[a, d]-cyclohepten-5,10-imine maleate (MK-801), on breathing pattern and ventilatory responses to hypoxia and hypercapnia in ground squirrels during natural hibernation. The hibernating squirrels breathed episodically and exhibited a robust ventilatory response to hypercapnia but not hypoxia. The breathing episode (not the individual breath) was the major regulated variable in the breathing pattern. Vagal blockade did not alter breathing on a breath-by-breath basis but did decrease the breaths per episode decreasing overall ventilation and abolished the hypercapnic ventilatory response. MK-801 increased the number of breaths per episode and the frequency of breathing during episodes but did not alter overall ventilation nor the hypercapnic ventilatory response. Combined treatment with MK-801 and vagal blockade abolished episodic breathing but also initiated arousal from hibernation. The data suggest that in golden-mantled ground squirrels hibernating at 5°C body temperature, vagal feedback and NMDA receptor mediated processes still modulate breathing. Whether they are responsible for clustering breaths into episodes is suggested but remains equivocal.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional and physiological effects of high-fat diets in finfish: effects on growth, immunity, lipid metabolism, and intestinal health: a review.","authors":"Hesham Eed Desouky, Nouran Mahmoud Sayed, Kenneth Prudence Abasubong, Ziping Zhang","doi":"10.1007/s00360-025-01626-z","DOIUrl":"https://doi.org/10.1007/s00360-025-01626-z","url":null,"abstract":"<p><p>High-fat diets (HFDs) are increasingly being studied in aquaculture because of their complex species-specific effects on fish physiology. While moderate fat levels can lower feed costs, supply essential fatty acids, and promote growth in some carnivorous and fast-growing species, excessive fat intake is linked to negative outcomes, such as impaired lipid metabolism, hepatic steatosis, immune suppression, and reduced growth. Although some studies have reported improved growth in zebrafish and other tolerant species, most finfish exhibited growth inhibition, metabolic dysfunction, and greater disease susceptibility under prolonged HFD exposure. Mechanistically, HFDs disrupt lipid homeostasis by downregulating lipolytic genes (e.g., cpt1a, pparα, and atgl) and upregulating lipogenic genes (e.g., srebp-1, fas, and acc), resulting in hepatic lipid accumulation. These shifts are associated with mitochondrial dysfunction, reduced fatty acid β-oxidation, oxidative stress, and activation of ER stress pathways such as ire1/xbp1. HFDs also stimulate inflammatory pathways through tlrs, nf-κb, and cytokines (il-6, tnf-α, and il-1β), contributing to immunometabolic imbalances. Additionally, HFDs negatively affect intestinal health by altering morphology, weakening barrier function, and disrupting microbiota composition, leading to poor nutrient absorption and increased infection risk. This review provides current evidence of HFD-induced changes in growth, immunity, lipid metabolism, mitochondrial function, and gut health in finfish. This emphasizes the importance of species-specific dietary fat optimization to improve feed efficiency, safeguard fish health, and ensure sustainable aquaculture practices.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144676666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial proteomic adaptations to daily torpor in the Djungarian hamster (Phodopus sungorus).","authors":"Anna Kovacs, Rob H Henning, Hjalmar Permentier, Justina C Wolters, Annika Herwig, Hjalmar R Bouma","doi":"10.1007/s00360-025-01625-0","DOIUrl":"https://doi.org/10.1007/s00360-025-01625-0","url":null,"abstract":"<p><p>Hibernation is an adaptive strategy that conserves energy in response to environmental challenges. While mitochondrial proteomic adaptations are well-documented in deep hibernators, the proteomic changes underlying daily torpor remain less clear. We investigated mitochondrial proteomic adaptations in the liver of a daily hibernator, the Djungarian hamster (Phodopus sungorus), across different hibernation phases. Hamsters were maintained under long-day (summer) or short-day photoperiods (winter), to induce torpor. Livers from summer, torpor, and interbout euthermia phases were analyzed by liquid chromatography-mass spectrometry with labelled standards of mitochondrial energy metabolism proteins, resulting in accurate quantitative proteomics. Differential protein regulation was assessed using empirical Bayes models with false discovery rate correction. Increased abundance of fatty acid oxidation enzymes during hibernation indicates a seasonal metabolic shift toward lipid utilization, similar to deep hibernators. Additionally, torpor featured elevated complex II subunits and tricarboxylic acid cycle enzymes representing evolutionary adaptations specific to daily torpor, likely to cater higher energy demands necessary to maintain torpid body temperature above 15 °C in near-freezing ambient temperatures. This represents evolutionary adaptations specific to daily torpor. Increased levels of the mitochondrial uncoupling-related solute carrier family 25 member 5 (SLC25A5) may be responsible for both thermogenesis and limiting production of reactive oxygen species. Furthermore, the selective upregulation of SOD2 during torpor underscores its critical role in mitigating reactive oxygen species accumulation during metabolic transitions. In summary, daily torpor exhibits unique mitochondrial proteomic adaptations that distinguish it from deep torpor, which may be necessary to enable torpor at body temperatures well above the ambient temperature.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematology of East Pacific green turtles (Chelonia mydas) in the Southern Gulf of California, Mexico: reference intervals of healthy turtles and intra-population comparisons.","authors":"Helena Fernández-Sanz, Jeffrey A Seminoff, Agnese Mancini, Jesús Salvador Lucero Romero, Delia Karen Oceguera Camacho, Eduardo Reséndiz","doi":"10.1007/s00360-025-01620-5","DOIUrl":"https://doi.org/10.1007/s00360-025-01620-5","url":null,"abstract":"<p><p>East Pacific green turtles (Chelonia mydas) inhabit tropical and subtropical waters along the western coast of the Americas. This population uses the Gulf of California, Mexico, as a primary area for feeding and refuge, where they face various stressors. This study aimed to establish hematological reference intervals for healthy green turtles in this area (n = 326), as well as evaluate seasonal variations in blood parameters and compare values between healthy turtles and individuals affected by fibropapillomatosis (n = 25). Reference intervals for hematological analytes were estimated following the American Society for Veterinary Clinical Pathology guidelines. Seasons significantly influenced hemoglobin, hematocrit and heterophils, which were lower during the cold season, while monocytes and eosinophils were significantly higher. These variations were attributed to physiological effects of cooler water temperatures, shorter photoperiods, reduced food intake, and immune response to seasonal stressors during the colder months. Turtles with visible fibropapillomatosis tumors were mildly affected, with tumor sizes not exceeding 5-cm diameter (tumor score 1). While significant alterations were observed in some hematological analytes (i.e., lower hemoglobin and hematocrit, and higher WBC, lymphocytes and heterophil: lymphocyte ratio), these values remained within the reference intervals estimated for healthy turtles. Additionally, no abnormalities commonly associated with severe fibropapillomatosis were observed, suggesting minimal structural or functional damage at tumor score 1 stage. This baseline information will help evaluate intra-population trends, assess potential impacts from future ecosystem changes, and develop effective conservation strategies for this threatened population.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass scaling of standard metabolic rate within and among individuals in Western mosquitofish (Gambusia affinis).","authors":"Shiqi Gao, Yanhong He, Bairu Du, Xinyi Li, Yangyang Jing, Yiping Luo","doi":"10.1007/s00360-025-01628-x","DOIUrl":"https://doi.org/10.1007/s00360-025-01628-x","url":null,"abstract":"<p><p>This study investigates the scaling exponent (b) of standard metabolic rate (SMR) in western mosquitofish, focusing on both among- and within-individual levels while exploring the influences of growth and sex on within-individual b values. For group-reared fish, the intraspecific b value was determined by analyzing the relationship between SMR and body mass across individuals of varying sizes and ages. For individually-reared fish, the SMR of each fish was measured five times between 60 and 150 days of age, and both among- and within-individual b values were calculated. The among- and within-individual b values of the individually-reared fish across ages did not differ significantly from the intraspecific b value of the group-reared fish. The among-individual b values showed a nonsignificant V-shaped change with increasing age in the individually-reared fish, suggesting that the ontogenetic decrease of b value related to growth slowdown can be reversed post-maturation. Although there was no significant difference in within-individual b values between females and males, greater variability was observed among females. Furthermore, the relationship between within-individual b values and specific growth rate displayed sexual dimorphism, with females exhibiting a stronger positive correlation, suggesting higher energy demands associated with both somatic and gonadal development compared to males.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of age-related inflammation and oxidative stress in two lemur species.","authors":"Elaine E Guevara, Nicholas M Grebe, Richard R Lawler, Anne Crowley, Savannah Lo, Elise N Paietta, Janet L Huebner, Virginia B Kraus, Christine M Drea","doi":"10.1007/s00360-025-01619-y","DOIUrl":"https://doi.org/10.1007/s00360-025-01619-y","url":null,"abstract":"<p><p>Oxidative damage and inflammation are mechanisms proposed to contribute to physiological senescence. Variation in oxidative damage and inflammation may reflect differential allocation of resources to reproduction and survival, contributing to differences in species-typical longevity and resulting from distinct, evolved life-history strategies. To investigate the link between molecular processes and physiological senescence, we compared urinary biomarkers of oxidative stress (8-isoprostane and 8-OHdG) and inflammation (neopterin) in a cross-sectional sample of two species that differ in life-history schedules: the relatively fast-paced ring-tailed lemur (Lemur catta; n = 41; ages = 1-32 years) and slow-paced Coquerel's sifaka (Propithecus coquereli; n = 49; ages = 1-27 years). Consistent with a faster life-history pace, ring-tailed lemurs showed significantly higher average levels of DNA damage than did sifakas (8-OHdG: ring-tailed lemur mean: 18.6 ± 10.3 ng/mg Cr, sifaka mean 8.0 ± 9.0 ng/mg Cr, p = 0.001). Species differences in lipid damage and inflammatory biomarkers were not significant (8-isoprostane: ring-tailed lemur mean: 0.5 ± 0.3 ng/mg Cr, sifaka mean: 0.3 ± 0.2 ng/mg Cr, p = 0.11), although sifakas tended to show greater inflammation (neopterin: ring-tailed lemur mean: 0.01 ± 0.02 ng/mg Cr, sifaka mean: 0.02 ± 0.02 ng/mg Cr; p = 0.14), which may reflect health challenges faced by this species in captivity. Contrary to our predictions, neither species showed age-related change in either marker of oxidative stress. Thus, although lemurs appear not to experience an increase in the rate of oxidative damage incurred with age, we cannot exclude the possibility that accumulated damage contributes to aging. Neither lemur species exhibited age-related change in inflammation; if anything, contrary to our prediction, ring-tailed lemurs showed marginal declines in inflammation with age. This finding, consistent with a few recent studies of other non-human primates, suggests that lemurs avoid the phenomenon of \"inflammaging\" widely observed in humans.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disaccharidases and alanyl aminopeptidase activities in intestine of a marine estuarine-dependent fish: differential responses to dopamine and histamine in juveniles of Mugil liza (Actiopterygii: Mugilidae).","authors":"Camila Albanesi, Mariano González-Castro, Alejandra López-Mañanes","doi":"10.1007/s00360-025-01622-3","DOIUrl":"https://doi.org/10.1007/s00360-025-01622-3","url":null,"abstract":"","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation under constant light or dark: a challenge to the pineal.","authors":"Alisa P Becin, Milica Trkulja, Aleksandra Bradasevic, Ivana Jokanovic, Dijana Z Travicic, Marija L J Medar, Silvana A Andric, Tatjana S Kostic","doi":"10.1007/s00360-025-01615-2","DOIUrl":"10.1007/s00360-025-01615-2","url":null,"abstract":"<p><p>The pineal gland synchronizes the body's circadian rhythms by producing melatonin in response to changes in the light-dark cycle. Our study evaluated how prolonged exposure to constant lighting (LL) or darkness (DD) affects pineal physiology by monitoring the transcription of genes critical for rhythmic endocrine activity. To achieve this, rats were exposed to LL, DD, or LD (control) from P21 to P90. LL and DD impacted the rats' physiology, as evidenced by the shift from a bimodal voluntary activity pattern to a free-running one. Serum melatonin and the transcription of genes encoding key enzymes involved in melatonin synthesis and adrenergic receptors changed their characteristic diurnal pattern observed in the LD group. The transcription of clock genes important for pineal rhythmicity was disturbed under both LL and DD: LL attenuated or flattened the expression of core clock genes, while DD showed a weaker effect and shifted the peak of expression. In LL, altered expression of clock genes was associated with increased transcription of regulators of mitochondrial biogenesis, and markers of mitophagy and mitochondrial dynamics, resulting in elevated ATP production. Oppositely, in DD conditions, ATP decreased. Principal Component Analysis (PCA) revealed distinct clusters comprising clock and mitochondria-related genes, indicating a close association between the circadian clock and mitochondrial function. These findings suggest that long-term exposure to LL environment poses more significant challenges to the pineal gland than DD. This might be associated with the heightened blood corticosterone levels observed in LL conditions, indicating potential chronobiological stress.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"379-392"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life in the slow lane: low rates of ubiquitin-dependent proteolysis in the heterothermic and heterometabolic tenrec, Tenrec ecaudatus.","authors":"Hector Aviles, Anna-Lena Vigil, Christy Strong, Frank van Breukelen","doi":"10.1007/s00360-025-01624-1","DOIUrl":"10.1007/s00360-025-01624-1","url":null,"abstract":"<p><p>Classical mammalian hibernators, such as the golden-mantled ground squirrel, exploit cold temperatures typical of torpor (~ 4 °C) as a robust cue for an imprecisely-coordinated depression of homeostatic processes such as protein synthesis and degradation. As a result, torpid metabolic rates may be 1/100th of active rates in ground squirrels. Tenrecs have profound thermal and metabolic plasticity; active tenrecs housed at low ambient temperatures may have body temperatures and resting metabolic rates similar to torpid tenrecs or more similar to that of the basal metabolic rate of comparably sized mammals. Importantly, tenrecs may be more or less active or torpid at both high and low body temperatures. Thus, temperature is likely an inappropriate cue for the regulation of homeostatic processes. Here, we demonstrate tenrecs have low but highly variable (~ tenfold) ubiquitylated protein concentrations, maintain robust ubiquitylation rates in the cold, have depressed proteolytic activities in the cold, and do not experience a marked depression of proteolysis in the torpid state. These data suggest an inability to regulate protein degradation when torpid or when body temperatures are reduced. We suggest that in ancestral-like mammals, a suitable approach was 'Life in the Slow Lane', wherein rates of processes like protein degradation (and presumably protein synthesis) were simply slow regardless of body temperature or torpor status. Low rates of processes are congruent with observed low metabolic rates and would help mitigate homeostatic mismatches incurred by a lack of coordination that might otherwise be deleterious.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"405-413"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The growth performance in different seasons of Homatula variegata (Dabry de Thiersant, 1874) in Northern Guizhou, China.","authors":"Gui Bang Xiao, Qi Wei Guo, Wei Xi Deng, Qi Qi Xiao, Lin Zhou, Hao Ran Zhang, Jia Lin, Ding Zhi Fang","doi":"10.1007/s00360-025-01621-4","DOIUrl":"10.1007/s00360-025-01621-4","url":null,"abstract":"<p><p>To provide knowledge on growth performance and data that are helpful for resource assessment of Homatula variegata (Dabry de Thiersant, 1874) living in northern Guizhou, China, the current study focused on the length-weight relationship (LWR), the growth pattern, the condition factor (CF), as well as the relationship between muscle metabolomics and growth in H. variegata. A total of 135 H. variegata were collected during 19 months using our patented hookless fishing tackles. The lengths (from the snout tip to the base of the caudal fin) and weights were measured, and the LWR and CF was calculated. Furthermore, the muscle tissues from fin tissues of 8 fish in winter were used for the analyses of metabolomics. The lengths and weights of 135 individuals varied by seasons, and there were opposite changes of the parameter a and the parameter b among different seasons. The parameters of LWR indicated that the growth pattern of H. variegata was the negative allometric growth. Furthermore, the CF of H. variegata was consistent with the seasonal variations of the lengths. Our results displayed that H. variegata exhibited better growth during spring and winter in northern Guizhou. Moreover, the positive correlation between triglycerides (TG) metabolites and CF found in the metabolomics analysis of muscle tissue in winter revealed the potential importance of lipid-rich food for the well-being and growth performance of H. variegata. Taken together, our findings provided basic information on the growth patterns and the relationship between metabolic characteristics and CF of H. variegata.</p>","PeriodicalId":56033,"journal":{"name":"Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology","volume":" ","pages":"293-303"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}