Gene Expression Patterns最新文献_第2页

Cell adhesion marker expression dynamics during fusion of the optic fissure 视裂融合过程中细胞粘附标记物的表达动态。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-10-14 DOI: 10.1016/j.gep.2023.119344

Holly Hardy, Joe Rainger

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Expression analysis of plvap in mouse heart development, homeostasis and injury plvap在小鼠心脏发育、稳态和损伤中的表达分析。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-28 DOI: 10.1016/j.gep.2023.119343

Yu Sui , Shan Kou , Kaixin Ge , Jinjun Cao , Chen Liu , Hui Zhang

{"title":"Expression analysis of plvap in mouse heart development, homeostasis and injury","authors":"Yu Sui , Shan Kou , Kaixin Ge , Jinjun Cao , Chen Liu , Hui Zhang","doi":"10.1016/j.gep.2023.119343","DOIUrl":"10.1016/j.gep.2023.119343","url":null,"abstract":"<div>Plasmalemma vesicle associated protein (PLVAP) is commonly considered to be specifically expressed in endothelial cells in which it localized to diaphragms of caveolae, fenestrae, and transendothelial channels. PLVAP is reported to be an important regulator of heart development and a novel target to promote cardiac repair in the ischemic heart. However, the dynamics of plvap expression in heart development, homeostasis and pathology have not been comprehensively described. In this study, we analyzed the temporal and spatial expression of plvap in mouse heart under different conditions. We found that, during embryonic and neonatal stages, PLVAP was detected in endocardial endothelial cells, epicardial mesothelial cells, and a small amount of coronary vascular endothelial cells. In adult heart, PLVAP was also identified in endocardial cells and a few coronary vascular endothelial cells. However, epicardial expression of PLVAP was lost during postnatal heart development and cannot be detected in mouse heart by immunostaining since 3-week-old. We also analyzed the expression of plvap in a model of cardiac hypertrophy and failure induced by transverse aortic constriction surgery, and identified expression of PLVAP in endocardial cells and coronary vascular endothelial cells in the injured heart. This study provides new evidence to better understand the role of plvap in mouse heart development and injury.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41162441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGFβ and GSK3 during pre-implantation development of goat embryos FGF、TGFβ和GSK3小分子抑制剂在山羊胚胎着床前发育过程中的多能性和胚胎谱系基因表达

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-09 DOI: 10.1016/j.gep.2023.119334

Mehdi Hajian, Shiva Rouhollahi Varnosfaderani, Farnoosh Jafarpour, Nima Tanhaei Vash, Mohammad Hossein Nasr-Esfahani

{"title":"Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGFβ and GSK3 during pre-implantation development of goat embryos","authors":"Mehdi Hajian, Shiva Rouhollahi Varnosfaderani, Farnoosh Jafarpour, Nima Tanhaei Vash, Mohammad Hossein Nasr-Esfahani","doi":"10.1016/j.gep.2023.119334","DOIUrl":"10.1016/j.gep.2023.119334","url":null,"abstract":"<div>Generating stable livestock pluripotent stem cells (PSCs) can be used for complex genome editing, cellular agriculture, gamete generation, regenerative medicine and in vitro breeding schemes. Over the past decade, significant progress has been made in characterizing pluripotency markers for livestock species. In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2–7 post-insemination in goat.Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the relative expression of NANOG, OCT4 and GATA4, while increased the expression of REX1. Among the combination of two molecules, only SB + CHIR combination significantly decreased the expression of GATA4, while the combination of the three molecules significantly decreases the expression of NANOG, SOX2 and CDX2.According to these results, the inhibition of the FGF signaling pathway, by PD may lead to blocking the hypoblast formation as observed by reduction of GATA4. OCT4 and NANOG expressions did not show signs of maintenance pluripotency. GATA4, NANOG and OCT4 in the PD group were downregulated and REX1 as EPI-marker was upregulated thus REX1 may be considered as a marker of EPI/ICM in goat.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10633629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The role of mip in the development of lens in zebrafish mip在斑马鱼晶状体发育中的作用。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119330

Mingyan He , Guangkai Zhou , Qinghong Lin , Nan Zhou

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CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration CRISPR/Cas9诱导的asap1a和asap1b共敲除突变斑马鱼表现出异常的胚胎发育和受损的中性粒细胞迁移。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119331

Jia Cui , Da Wen , Liqing Wang , Chaoqun Meng , Yuhuan Wang , Zhonghua Zhao , Changxin Wu

{"title":"CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration","authors":"Jia Cui , Da Wen , Liqing Wang , Chaoqun Meng , Yuhuan Wang , Zhonghua Zhao , Changxin Wu","doi":"10.1016/j.gep.2023.119331","DOIUrl":"10.1016/j.gep.2023.119331","url":null,"abstract":"<div>ASAP1 (Arf-GAP with SH3 domain, the ankyrin repeat and the PH domain) is the GTPase activating protein of the small G protein Arf. To understand more about the physiological functions of ASAP1 in vivo, we chose to use the zebrafish as an animal model, and analyzed the characterization of asap1 using loss-of-function studies. Here, two isoforms in zebrafish, asap1a and asap1b, were found to be homologous to human ASAP1, and the gene knockout zebrafish lines for asap1a and asap1b were established using the CRISPR/Cas9 technique with different insertions and deletions of bases. Zebrafish with asap1a and asap1b co-knockout showed a significant reduction in survival and hatching rates, as well as an increase in malformation rates during the early stages of development, while the asap1a or asap1b single knockout mutants did not affect the growth and development of individual zebrafish. Exploring the gene expression compensation between asap1a and asap1b using qRT-PCR, we found that asap1b had increased expression when asap1a was knocked out, showing a clear compensatory effect against asap1a knockout; In turn, asap1a did not have detectable compensating expression after asap1b knockout. Furthermore, the co-knockout homozygous mutants displayed impaired neutrophil migration to Mycobacterium marinum infection, and showed an increased bacterial load. Together, these are the first inherited asap1a and/or asap1b mutant zebrafish lines by the CRISPR/Cas9 gene editing approach, and by serving as useful models, they can significantly contribute to better annotation and follow-up physiological studies of human ASAP1.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Editorial note 编辑注意

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119335

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Spatiotemporal expression patterns of R-spondins and their receptors, Lgrs, in the developing mouse telencephalon R-反应蛋白及其受体Lgrs在发育中的小鼠端脑中的时空表达模式。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119333

Keisuke Watanabe , Masao Horie , Manabu Hayatsu , Yoshikazu Mikami , Noboru Sato

{"title":"Spatiotemporal expression patterns of R-spondins and their receptors, Lgrs, in the developing mouse telencephalon","authors":"Keisuke Watanabe , Masao Horie , Manabu Hayatsu , Yoshikazu Mikami , Noboru Sato","doi":"10.1016/j.gep.2023.119333","DOIUrl":"10.1016/j.gep.2023.119333","url":null,"abstract":"<div>Development of the mammalian telencephalon, which is the most complex region of the central nervous system, is precisely orchestrated by many signaling molecules. Wnt signaling derived from the cortical hem, a signaling center, is crucial for telencephalic development including cortical patterning and the induction of hippocampal development. Secreted protein R-spondin (Rspo) 1–4 and their receptors, leucine-rich repeat-containing G-protein-coupled receptor (Lgr) 4–6, act as activators of Wnt signaling. Although Rspo expression in the hem during the early stages of cortical development has been reported, comparative expression analysis of Rspos and Lgr4–6 has not been performed. In this study, we examined the detailed spatiotemporal expression patterns of Rspo1–4 and Lgr4–6 in the embryonic and postnatal telencephalon to elucidate their functions. In the embryonic day (E) 10.5–14.5 telencephalon, Rspo1–3 were prominently expressed in the cortical hem. Among their receptors, Lgr4 was observed in the ventral telencephalon, and Lgr6 was highly expressed throughout the telencephalon at the same stages. This suggests that Rspo1–3 and Lgr4 initially regulate telencephalic development in restricted regions, whereas Lgr6 functions broadly. From the late embryonic stage, the expression areas of Rspo1–3 and Lgr4–6 dramatically expanded; their expression was found in the neocortex and limbic system, such as the hippocampus, amygdala, and striatum. Increased Rspo and Lgr expression from the late embryonic stages suggests broad roles of Rspo signaling in telencephalic development. Furthermore, the Lgr+ regions were located far from the Rspo+ regions, especially in the E10.5–14.5 ventral telencephalon, suggesting that Lgrs act via a Rspo-independent pathway.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10648888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Comparative expression analysis of TEADs and their splice variants in mouse embryonic stem cells” [Gene Expr Patterns 47 (2022) 119302/36516960] “TEADs及其剪接变体在小鼠胚胎干细胞中的比较表达分析”的更正[Gene Expr Patterns 47 (2022) 119302/36516960]

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119332

Yuda Cheng , Yang Xiao , Yan Ruan , Jiali Wang , Yanping Tian , Jiaxiang Xiong , Jiaqi Wang , Fengsheng Wang , Chen Zhang , Yixiao Xu , Lianlian Liu , Meng Yu , Jiangjun Wang , Binyu Zhao , Yue Zhang , Ran Yang , Yi Yang , Zhongxiang Yao , Rui Jian , Lan Xiao , Junlei Zhang

引用次数: 0

Fibroblast growth factor pathway component expression in the regenerating zebrafish fin 成纤维细胞生长因子通路组分在再生斑马鱼鳍中的表达

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-06-01 DOI: 10.1016/j.gep.2023.119307

Nicole Cudak , Alejandra Cristina López-Delgado , Sebastian Keil , Franziska Knopf

{"title":"Fibroblast growth factor pathway component expression in the regenerating zebrafish fin","authors":"Nicole Cudak , Alejandra Cristina López-Delgado , Sebastian Keil , Franziska Knopf","doi":"10.1016/j.gep.2023.119307","DOIUrl":"10.1016/j.gep.2023.119307","url":null,"abstract":"<div>Adult zebrafish regenerate their appendages (fins) after amputation including the regeneration of bone structures (fin rays). Fibroblast growth factor (Fgf) signaling, which is involved in morphogenetic processes during development, has been shown to be essential for the process of fin regeneration. Moreover, mutations in Fgf pathway component genes lead to abnormal skeletal growth in teleosts and mammals, including humans, illustrating the importance of Fgf signaling in the growth control of tissues. Here, we revisited Fgf signaling pathway component expression by RNA in situ hybridization to test for the expression of about half of the ligands and all receptors of the pathway in the regenerating zebrafish fin. Expression patterns of fgf7, fgf10b, fgf12b, fgf17b and fgfr1b have not been reported in the literature before. We summarize and discuss known and novel localization of expression and find that all five Fgf receptors (fgfr1a, fgfr1b, fgfr2, fgfr3 and fgfr4) and most of the tested ligands are expressed in specific regions of the regenerate. Our work provides a basis to study domain specific functions of Fgf signaling in the regenerating teleost appendage.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9663639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Transcription factor cellular promoter 2 is required for upstream binding protein 1 -mediated angiogenesis 转录因子细胞启动子2是上游结合蛋白1介导的血管生成所必需的

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-06-01 DOI: 10.1016/j.gep.2023.119308

Yanyan Ren , YaneYang , Qingbo Lu , Qiang Wang , Gentao Lu , Yanli Wei , Jiaqi Zhou

{"title":"Transcription factor cellular promoter 2 is required for upstream binding protein 1 -mediated angiogenesis","authors":"Yanyan Ren , YaneYang , Qingbo Lu , Qiang Wang , Gentao Lu , Yanli Wei , Jiaqi Zhou","doi":"10.1016/j.gep.2023.119308","DOIUrl":"10.1016/j.gep.2023.119308","url":null,"abstract":"<div><h3>Objective</h3>Angiogenesis is a key process of repairing tissue damage, and it is regulated by the delicate balance between anti-angiogenesis factors. In the present study, we investigate whether transcription factor cellular promoter 2 (TFCP2) is required for upstream binding protein 1 (UBP1)-mediated angiogenesis.</div><div><h3>Methods</h3>Levels of UBP1 and TFCP2 in human umbilical vein endothelial cells (HUVECs) are detected by quantitative polymerase chain reaction (q-PCR) and Western blotting (WB). Effects of UBP1 on angiogenesis and migration are detected by tube-like network formation on matrigel assay and scratch assay. The interaction between UBP1 and TFCP2 is predicted and verified by STRING and Co-immunoprecipitation (Co-IP).</div><div><h3>Results</h3>Firstly, the UBP1 expression level was up-regulated in the stimuli of vascular endothelial growth factor (VEGF) in HUVECs, and the knockdown of UBP1 inhibited angiogenesis and migration of HUVECs. Then, UBP1 interacted with TFCP2. Besides, the TFCP2 expression level was up-regulated in VEGF-stimulated HUVECs. Furthermore, knockdown of TFCP2 inhibited angiogenesis and migration in VEGF-stimulated HUVECs, and down-regulation of UBP1 enhanced the inhibition.</div><div><h3>Conclusion</h3>TFCP2 also plays a key role in UBP1 mediated angiogenesis of HUVECs stimulated by VEGF. These findings will provide a new theoretical basis for the treatment of angiogenic diseases.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0