Gene Expression Patterns最新文献_第2页

Transcriptional regulation of the alternative sex hormone-binding globulin promoter by KLF4. KLF4 对替代性荷尔蒙结合球蛋白启动子的转录调控。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2024-03-07 DOI: 10.1016/j.gep.2024.119357

Warren M Meyers

{"title":"Transcriptional regulation of the alternative sex hormone-binding globulin promoter by KLF4.","authors":"Warren M Meyers","doi":"10.1016/j.gep.2024.119357","DOIUrl":"https://doi.org/10.1016/j.gep.2024.119357","url":null,"abstract":"In most mammals the major site of sex hormone-binding globulin (SHBG) synthesis is the liver wherefrom it is secreted into the bloodstream and is the primary determinant of sex steroid access to target tissues. The minor site of SHBG synthesis is the testis and in lower mammals testicular SHBG has long been known to be synthesized and secreted by Sertoli cells. However, human testicular SHBG is expressed in developing germ cells from an upstream alternative promoter (altP-SHBG). Transcripts arising from this region comprise an alternative first exon (1A) with the resultant protein confined to the acrosomal compartment of the mature spermatozoa. I have dissected the regulatory components of the alternative SHBG promoter and identified motifs that are required for optimal transcriptional activity from this region. Transcriptional activity is driven by two CACCC elements that appear to be functionally redundant. The transcription factor KLF4 interacts with promoter the region spanning these elements in vivo. Knockdown of Klf4 results in decreased altP-SHBG activity, while Klf4 overexpression relieves the effects of knockdown. Based on their shared patterns of expression in vivo, I conclude that KLF4 is a transcriptional regulator of SHBG in male germ cells.","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":" ","pages":"119357"},"PeriodicalIF":1.2,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression analysis of oxidative stress-related genes in the apical, middle, and basal turns of the cochlea 耳蜗顶端、中部和基底转折处氧化应激相关基因的基因表达分析

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2024-03-01 DOI: 10.1016/j.gep.2024.119356

Yang Yang, Xin Chen, Chaoyong Tian, Bei Fan, Xiaogang An, Zhenzhen Liu, Qiong Li, Wenjuan Mi, Ying Lin, Dingjun Zha

{"title":"Gene expression analysis of oxidative stress-related genes in the apical, middle, and basal turns of the cochlea","authors":"Yang Yang, Xin Chen, Chaoyong Tian, Bei Fan, Xiaogang An, Zhenzhen Liu, Qiong Li, Wenjuan Mi, Ying Lin, Dingjun Zha","doi":"10.1016/j.gep.2024.119356","DOIUrl":"https://doi.org/10.1016/j.gep.2024.119356","url":null,"abstract":"<div>It can be observed from aminoglycoside-induced hair cell damage that the cochlea basal turn is more susceptible to trauma than the apex. Drug-induced hearing loss is closely related to oxidative damage. The basilar membrane directly exposed to these ototoxic drugs exhibits differences in damage, indicating that there is an inherent difference in the sensitivity to oxidative damage from the apex to the base of the cochlea. It has been reported that the morphology and characteristics of the cochlea vary from the apex to the base. Therefore, we investigated oxidative stress-related gene expression profiles in the apical, middle, and basal turns of the cochlea. The Oxidative Stress RT2 Profiler™ PCR Array revealed that three of the 84 genes (Mb, Mpo, and Ncf1) were upregulated in the middle turn compared to their level in the apical turn. Moreover, eight genes (Mb, Duox1, Ncf1, Ngb, Fmo2, Gpx3, Mpo, and Gstk1) were upregulated in the basal turn compared to their level in the apical turn. The qPCR verification data were similar to that of the PCR Array. We found that MPO was expressed in the rat cochlea and protected against gentamicin-induced hair cell death. This study summarized the data for the gradient of expression of oxidative stress-related genes in the cochlea and found potential candidate targets for prevention of ototoxic deafness, which may provide new insights for cochlear pathology.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"51 ","pages":"Article 119356"},"PeriodicalIF":1.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140013922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryonic expression patterns of TBL1 family in zebrafish TBL1 家族在斑马鱼胚胎期的表达模式

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2024-01-23 DOI: 10.1016/j.gep.2024.119355

Yuanqi Jia , Qiu Jiang , Shuna Sun

{"title":"Embryonic expression patterns of TBL1 family in zebrafish","authors":"Yuanqi Jia , Qiu Jiang , Shuna Sun","doi":"10.1016/j.gep.2024.119355","DOIUrl":"10.1016/j.gep.2024.119355","url":null,"abstract":"<div>Except the addition of TBL1Y in human, transducing beta like 1 (TBL1) family mainly consists of two members TBL1X and TBL1XR1, taking part in multiple intracellular signaling pathways such as Wnt/β-catenin and NF-κB in cancer progression. However, the gene expression patterns of this family during embryonic development remain largely unknown. Here we took advantage of zebrafish model to characterize the spatial and temporal expression patterns of TBL1 family genes including tbl1x, tbl1xr1a and tbl1xr1b. The in situ hybridization studies of gene expression showed robust expressions of tbl1x and tbl1xr1b as maternal transcripts except tbl1xr1a. As the embryo develops, zygotic expressions of all TBL1 family members occur and have a redundant and broad pattern including in brain, neural retina, pharyngeal arches, otic vesicles, and pectoral fins. Ubiquitous expression of all family members were ranked from the strongest to the weakest: tbl1xr1a, tbl1x, and tbl1xr1b. In addition, one tbl1xr1a transcript tbl1xr1a202 showed unique and rich expression in the developing heart and lateral line neuromasts. Overall, all members of zebrafish TBL1 family shared numerous similarities and exhibited certain distinctions in the expression patterns, indicating that they might have redundant and exclusive functions to be further explored.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"51 ","pages":"Article 119355"},"PeriodicalIF":1.2,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139558299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome complex components 1 and 2 are vital for early mammalian development 外泌体复合物组分1和2对哺乳动物的早期发育至关重要。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-11-07 DOI: 10.1016/j.gep.2023.119346

Sanjana Srinivasan, Xinjian He, Sarah Mirza, Jesse Mager

{"title":"Exosome complex components 1 and 2 are vital for early mammalian development","authors":"Sanjana Srinivasan, Xinjian He, Sarah Mirza, Jesse Mager","doi":"10.1016/j.gep.2023.119346","DOIUrl":"10.1016/j.gep.2023.119346","url":null,"abstract":"<div>Exosome Complex Components 1 and 2 (EXOSC1 and 2) are two proteins in the RNA Exosome complex whose main function is 5’ → 3’ RNA degradation and processing. The RNA exosome complex is comprised of nine subunits that form two separate components: the S1/KH cap and the PH-core. EXOSC1 and 2 are both part of the S1/KH cap and are involved in binding nascent RNA. As part of a systemic characterization of early lethal alleles produced by the Knockout Mouse Project, we have examined Exosc1 and Exosc2 homozygous null (mutant) embryos to determine developmental and molecular phenotypes of embryos lacking their functions. Our studies reveal that Exosc1 null embryos implant and form an egg cylinder but are developmentally delayed and fail to initiate gastrulation by embryonic day 7.5. In contrast, Exosc2 null embryos are lethal during peri-implantation stages, and while they do form a morphologically normal blastocyst at E3.5, they cannot be recovered at post-implantation stages. We show the absence of stage-specific developmental and altered lineage-specification in both Exosc1 and Exosc2 mutant embryos and conclude that these genes are essential for the successful progression through early mammalian development.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"51 ","pages":"Article 119346"},"PeriodicalIF":1.2,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71523485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental expression of peroxiredoxin gene family in early embryonic development of Xenopus tropicalis 热带爪蟾早期胚胎发育过程中过氧氧化还原素基因家族的发育表达。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-10-14 DOI: 10.1016/j.gep.2023.119345

Linke Zhong , Tingting Fu , Chengdong Wang , Xufeng Qi , Wai-Yee Chan , Dongqing Cai , Hui Zhao

引用次数: 1

Cell adhesion marker expression dynamics during fusion of the optic fissure 视裂融合过程中细胞粘附标记物的表达动态。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-10-14 DOI: 10.1016/j.gep.2023.119344

Holly Hardy, Joe Rainger

引用次数: 0

Expression analysis of plvap in mouse heart development, homeostasis and injury plvap在小鼠心脏发育、稳态和损伤中的表达分析。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-28 DOI: 10.1016/j.gep.2023.119343

Yu Sui , Shan Kou , Kaixin Ge , Jinjun Cao , Chen Liu , Hui Zhang

{"title":"Expression analysis of plvap in mouse heart development, homeostasis and injury","authors":"Yu Sui , Shan Kou , Kaixin Ge , Jinjun Cao , Chen Liu , Hui Zhang","doi":"10.1016/j.gep.2023.119343","DOIUrl":"10.1016/j.gep.2023.119343","url":null,"abstract":"<div>Plasmalemma vesicle associated protein (PLVAP) is commonly considered to be specifically expressed in endothelial cells in which it localized to diaphragms of caveolae, fenestrae, and transendothelial channels. PLVAP is reported to be an important regulator of heart development and a novel target to promote cardiac repair in the ischemic heart. However, the dynamics of plvap expression in heart development, homeostasis and pathology have not been comprehensively described. In this study, we analyzed the temporal and spatial expression of plvap in mouse heart under different conditions. We found that, during embryonic and neonatal stages, PLVAP was detected in endocardial endothelial cells, epicardial mesothelial cells, and a small amount of coronary vascular endothelial cells. In adult heart, PLVAP was also identified in endocardial cells and a few coronary vascular endothelial cells. However, epicardial expression of PLVAP was lost during postnatal heart development and cannot be detected in mouse heart by immunostaining since 3-week-old. We also analyzed the expression of plvap in a model of cardiac hypertrophy and failure induced by transverse aortic constriction surgery, and identified expression of PLVAP in endocardial cells and coronary vascular endothelial cells in the injured heart. This study provides new evidence to better understand the role of plvap in mouse heart development and injury.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"50 ","pages":"Article 119343"},"PeriodicalIF":1.2,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41162441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGFβ and GSK3 during pre-implantation development of goat embryos FGF、TGFβ和GSK3小分子抑制剂在山羊胚胎着床前发育过程中的多能性和胚胎谱系基因表达

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-09 DOI: 10.1016/j.gep.2023.119334

Mehdi Hajian, Shiva Rouhollahi Varnosfaderani, Farnoosh Jafarpour, Nima Tanhaei Vash, Mohammad Hossein Nasr-Esfahani

{"title":"Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGFβ and GSK3 during pre-implantation development of goat embryos","authors":"Mehdi Hajian, Shiva Rouhollahi Varnosfaderani, Farnoosh Jafarpour, Nima Tanhaei Vash, Mohammad Hossein Nasr-Esfahani","doi":"10.1016/j.gep.2023.119334","DOIUrl":"10.1016/j.gep.2023.119334","url":null,"abstract":"<div>Generating stable livestock pluripotent stem cells (PSCs) can be used for complex genome editing, cellular agriculture, gamete generation, regenerative medicine and in vitro breeding schemes. Over the past decade, significant progress has been made in characterizing pluripotency markers for livestock species. In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2–7 post-insemination in goat.Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the relative expression of NANOG, OCT4 and GATA4, while increased the expression of REX1. Among the combination of two molecules, only SB + CHIR combination significantly decreased the expression of GATA4, while the combination of the three molecules significantly decreases the expression of NANOG, SOX2 and CDX2.According to these results, the inhibition of the FGF signaling pathway, by PD may lead to blocking the hypoblast formation as observed by reduction of GATA4. OCT4 and NANOG expressions did not show signs of maintenance pluripotency. GATA4, NANOG and OCT4 in the PD group were downregulated and REX1 as EPI-marker was upregulated thus REX1 may be considered as a marker of EPI/ICM in goat.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"50 ","pages":"Article 119334"},"PeriodicalIF":1.2,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10633629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of mip in the development of lens in zebrafish mip在斑马鱼晶状体发育中的作用。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119330

Mingyan He , Guangkai Zhou , Qinghong Lin , Nan Zhou

引用次数: 0

CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration CRISPR/Cas9诱导的asap1a和asap1b共敲除突变斑马鱼表现出异常的胚胎发育和受损的中性粒细胞迁移。

IF 1.2 4区生物学

Gene Expression Patterns Pub Date : 2023-09-01 DOI: 10.1016/j.gep.2023.119331

Jia Cui , Da Wen , Liqing Wang , Chaoqun Meng , Yuhuan Wang , Zhonghua Zhao , Changxin Wu

{"title":"CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration","authors":"Jia Cui , Da Wen , Liqing Wang , Chaoqun Meng , Yuhuan Wang , Zhonghua Zhao , Changxin Wu","doi":"10.1016/j.gep.2023.119331","DOIUrl":"10.1016/j.gep.2023.119331","url":null,"abstract":"<div>ASAP1 (Arf-GAP with SH3 domain, the ankyrin repeat and the PH domain) is the GTPase activating protein of the small G protein Arf. To understand more about the physiological functions of ASAP1 in vivo, we chose to use the zebrafish as an animal model, and analyzed the characterization of asap1 using loss-of-function studies. Here, two isoforms in zebrafish, asap1a and asap1b, were found to be homologous to human ASAP1, and the gene knockout zebrafish lines for asap1a and asap1b were established using the CRISPR/Cas9 technique with different insertions and deletions of bases. Zebrafish with asap1a and asap1b co-knockout showed a significant reduction in survival and hatching rates, as well as an increase in malformation rates during the early stages of development, while the asap1a or asap1b single knockout mutants did not affect the growth and development of individual zebrafish. Exploring the gene expression compensation between asap1a and asap1b using qRT-PCR, we found that asap1b had increased expression when asap1a was knocked out, showing a clear compensatory effect against asap1a knockout; In turn, asap1a did not have detectable compensating expression after asap1b knockout. Furthermore, the co-knockout homozygous mutants displayed impaired neutrophil migration to Mycobacterium marinum infection, and showed an increased bacterial load. Together, these are the first inherited asap1a and/or asap1b mutant zebrafish lines by the CRISPR/Cas9 gene editing approach, and by serving as useful models, they can significantly contribute to better annotation and follow-up physiological studies of human ASAP1.</div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"49 ","pages":"Article 119331"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0