The expression pattern of Wnt6, Wnt10A, and HOXA13 during regenerating tails of Gekko Japonicus

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2024-08-10 DOI:10.1016/j.gep.2024.119374

Zhen Li, Mingxuan Li, Shuai Huang, Jing Yu, Mei Liu, Yan Liu, Man Xu

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引用次数: 0

Abstract

Wnt signal is crucial to correctly regenerate tissues along the original axis in many animals. Lizards are able to regenerate their tails spontaneously, while the anterior-posterior axis information required for the successful regeneration is still elusive. In this study, we investigated the expression pattern of Wnt ligands and HOX genes during regeneration. The results of in situ hybridization revealed that Wnt6 and Wnt10A mRNA levels are higher in wound epithelium (WE) than that in blastema during regeneration. In addition, we showed that Wnt agonist positively regulated the expression of HOXA13 in cultured blastema cells, while did not show similar effect on that of HOXB13, HOXC13 and HOXD13. Finally, we found that HOXA13 showed a gradient level along the anterior-posterior axis of regenerated blastema, with higher level at the caudal end. These data proposed that Wnt6, Wnt10A and HOXA13 might play an important role in establishing distal position for regeneration.

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日本壁虎再生尾部过程中 Wnt6 和 HOXA13 的表达模式。

在许多动物中，Wnt 信号对于组织沿着原轴线正确再生至关重要。蜥蜴能够自发地再生其尾巴，但成功再生所需的前后轴信息仍然难以捉摸。本研究调查了再生过程中 Wnt 配体和 HOX 基因的表达模式。原位杂交结果显示，在再生过程中，伤口上皮（WE）的Wnt6水平高于胚泡。此外，我们还发现Wnt激动剂能正向调节HOXA13在培养的胚泡细胞中的表达，而对HOXB13、HOXC13和HOXD13的表达没有类似的影响。最后，我们发现HOXA13在再生胚泡中沿前后轴呈梯度表达，尾端表达水平较高。这些数据表明，Wnt6和HOXA13可能在建立再生的远端位置中发挥了重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation