Briefings in Functional Genomics最新文献

{"title":"Correction to: STAT3-dependent long non-coding RNA Lncenc1 contributes to mouse ES cells pluripotency via stabilizing Klf4 mRNA.","authors":"","doi":"10.1093/bfgp/elaf012","DOIUrl":"10.1093/bfgp/elaf012","url":null,"abstract":"","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":"24 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic analysis of the transcriptional landscape of melanoma reveals drug-target expression plasticity.","authors":"Brad Balderson, Mitchell Fane, Tracey J Harvey, Michael Piper, Aaron Smith, Mikael Bodén","doi":"10.1093/bfgp/elad055","DOIUrl":"10.1093/bfgp/elad055","url":null,"abstract":"<p><p>Metastatic melanoma originates from melanocytes of the skin. Melanoma metastasis results in poor treatment prognosis for patients and is associated with epigenetic and transcriptional changes that reflect the developmental program of melanocyte differentiation from neural crest stem cells. Several studies have explored melanoma transcriptional heterogeneity using microarray, bulk and single-cell RNA-sequencing technologies to derive data-driven models of the transcriptional-state change which occurs during melanoma progression. No study has systematically examined how different models of melanoma progression derived from different data types, technologies and biological conditions compare. Here, we perform a cross-sectional study to identify averaging effects of bulk-based studies that mask and distort apparent melanoma transcriptional heterogeneity; we describe new transcriptionally distinct melanoma cell states, identify differential co-expression of genes between studies and examine the effects of predicted drug susceptibilities of different cell states between studies. Importantly, we observe considerable variability in drug-target gene expression between studies, indicating potential transcriptional plasticity of melanoma to down-regulate these drug targets and thereby circumvent treatment. Overall, observed differences in gene co-expression and predicted drug susceptibility between studies suggest bulk-based transcriptional measurements do not reliably gauge heterogeneity and that melanoma transcriptional plasticity is greater than described when studies are considered in isolation.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139106948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of artificial intelligence-based brain age estimation and its applications for related diseases.","authors":"Mohamed Azzam, Ziyang Xu, Ruobing Liu, Lie Li, Kah Meng Soh, Kishore B Challagundla, Shibiao Wan, Jieqiong Wang","doi":"10.1093/bfgp/elae042","DOIUrl":"10.1093/bfgp/elae042","url":null,"abstract":"<p><p>The study of brain age has emerged over the past decade, aiming to estimate a person's age based on brain imaging scans. Ideally, predicted brain age should match chronological age in healthy individuals. However, brain structure and function change in the presence of brain-related diseases. Consequently, brain age also changes in affected individuals, making the brain age gap (BAG)-the difference between brain age and chronological age-a potential biomarker for brain health, early screening, and identifying age-related cognitive decline and disorders. With the recent successes of artificial intelligence in healthcare, it is essential to track the latest advancements and highlight promising directions. This review paper presents recent machine learning techniques used in brain age estimation (BAE) studies. Typically, BAE models involve developing a machine learning regression model to capture age-related variations in brain structure from imaging scans of healthy individuals and automatically predict brain age for new subjects. The process also involves estimating BAG as a measure of brain health. While we discuss recent clinical applications of BAE methods, we also review studies of biological age that can be integrated into BAE research. Finally, we point out the current limitations of BAE's studies.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A survey of biclustering and clustering methods in clustering different types of single-cell RNA sequencing data.","authors":"Chaowang Lan, Xiaoqi Tang, Caihua Liu","doi":"10.1093/bfgp/elaf010","DOIUrl":"10.1093/bfgp/elaf010","url":null,"abstract":"<p><p>Single-cell RNA sequencing (scRNA-seq) technology has garnered considerable attention as it enables the exploration of cellular heterogeneity from a single-cell perspective. Various unsupervised methods, such as biclustering and clustering methods, offer a theoretical foundation for understanding the structure and function of cells. However, accurately identifying cell subtypes within complex scRNA-seq data remains challenging. To evaluate the current development status; summarize the strengths, weaknesses, and improvement strategies of unsupervised methods; and provide guidelines for future research, we surveyed five biclustering and 21 clustering methods applied to different types of scRNA-seq datasets. We employed three external and two internal metrics to determine clustering performance on 10 publicly available real datasets. Dataset properties are quantified from six perspectives to discover the most suitable biclustering or clustering methods. The results of this survey indicate that biclustering methods are effective for identifying local consistency or for deeply mining partially annotated datasets. Conversely, clustering methods are more suitable for dealing with unknown datasets. This survey aids in identifying cellular heterogeneity by recommending appropriate methods based on different dataset characteristics.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":"24 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12342763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144838650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EnsembleSE: identification of super-enhancers based on ensemble learning.","authors":"Wenying He, Jialu Xu, Yun Zuo, Yude Bai, Fei Guo","doi":"10.1093/bfgp/elaf003","DOIUrl":"https://doi.org/10.1093/bfgp/elaf003","url":null,"abstract":"<p><p>Super-enhancers (SEs) are typically located in the regulatory regions of genes, driving high-level gene expression. Identifying SEs is crucial for a deeper understanding of gene regulatory networks, disease mechanisms, and the development and physiological processes of organisms, thus exerting a profound impact on research and applications in the life sciences field. Traditional experimental methods for identifying SEs are costly and time-consuming. Existing methods for predicting SEs based solely on sequence data use deep learning for feature representation and have achieved good results. However, they overlook biological features related to physicochemical properties, leading to low interpretability. Additionally, the complex model structure often requires extensive labeled data for training, which limits their further application in biological data. In this paper, we integrate the strengths of different models and proposes an ensemble model based on an integration strategy to enhance the model's generalization ability. It designs a multi-angle feature representation method that combines local structure and global information to extract high-dimensional abstract relationships and key low-dimensional biological features from sequences. This enhances the effectiveness and interpretability of the model's input features, providing technical support for discovering cell-specific and species-specific patterns of SEs. We evaluated the performance on both mouse and human datasets using five metrics, including area under the receiver operating characteristic curve accuracy, and others. Compared to the latest models, EnsembleSE achieved an average improvement of 4.5% in F1 score and an average improvement of 8.05% in recall, demonstrating the robustness and adaptability of the model on a unified test set. Source codes are available at https://github.com/2103374200/EnsembleSE-main.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":"24 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between genomic variants and DNA methylation in FLT3 mutant acute myeloid leukemia.","authors":"Bac Dao, Van Ngu Trinh, Huy V Nguyen, Hoa L Nguyen, Thuc Duy Le, Phuc Loi Luu","doi":"10.1093/bfgp/elae028","DOIUrl":"10.1093/bfgp/elae028","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) is a type of blood cancer with diverse genetic variations and DNA methylation alterations. By studying the interaction of gene mutations, expression, and DNA methylation, we aimed to gain valuable insights into the processes that lead to block differentiation in AML. We analyzed TCGA-LAML data (173 samples) with RNA sequencing and DNA methylation arrays, comparing FLT3 mutant (48) and wild-type (125) cases. We conducted differential gene expression analysis using cBioPortal, identified DNA methylation differences with ChAMP tool, and correlated them with gene expression changes. Gene set enrichment analysis (g:Profiler) revealed significant biological processes and pathways. ShinyGo and GeneCards were used to find potential transcription factors and their binding sites among significant genes. We found significant differentially expressed genes (DEGs) negatively correlated with their most significant methylation probes (Pearson correlation coefficient of -0.49, P-value <0.001) between FLT3 mutant and wild-type groups. Moreover, our exploration of 450 k CpG sites uncovered a global hypo-methylated status in 168 DEGs. Notably, these methylation changes were enriched in the promoter regions of Homebox superfamily gene, which are crucial in transcriptional-regulating pathways in blood cancer. Furthermore, in FLT3 mutant AML patient samples, we observed overexpress of WT1, a transcription factor known to bind homeobox gene family. This finding suggests a potential mechanism by which WT1 recruits TET2 to demethylate specific genomic regions. Integrating gene expression and DNA methylation analyses shed light on the impact of FLT3 mutations on cancer cell development and differentiation, supporting a two-hit model in AML. This research advances understanding of AML and fosters targeted therapeutic strategy development.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual oncogenic roles of TPD52 and TPD52L2 in gastric cancer progression via PI3K/AKT activation and immunosuppressive microenvironment remodeling.","authors":"Hailong Li, Xiaqing Gao, Shuangming Guo, Shenfei Gao, Chunting Yang, Rong Su, Zhe Jing, Shuping Qiu, Ping Tang, Jing Han","doi":"10.1093/bfgp/elaf015","DOIUrl":"10.1093/bfgp/elaf015","url":null,"abstract":"<p><strong>Aim: </strong>TPD52 (tumor protein D52) and TPD52L2 (tumor protein D52-like 2), members of the TPD52 gene family, have been implicated in multiple malignancies. However, their roles in gastric cancer (GC) remain elusive. Herein, we integrated multiomics analyses and experimental validation to elucidate their prognostic and functional significance in GC.</p><p><strong>Methods: </strong>Utilizing The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and tissue microarray datasets, we analyzed TPD52/TPD52L2 expression patterns in patients with GC. Survival analysis, Cox regression, and nomogram construction were performed to assess prognostic value. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analysis and immune infiltration evaluation (Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts‌/Estimation of STromal and Immune cells in MAlignant Tumour tissues using Expression data) (CIBERSORTx/ESTIMATE) were conducted to explore the molecular mechanisms involved. In vitro experiments (cell proliferation, migration, invasion, and apoptosis assays) were performed via lentivirus-mediated gene knockdown in gastric cancer cell lines AGS and MKN45 cells.</p><p><strong>Results: </strong>TPD52 and TPD52L2 were significantly overexpressed in GC tissues compared with their normal counterparts. Elevated TPD52L2 expression was significantly associated with advanced Tumor, Node, Metastasis (TNM) stage and independently predicted reduced overall survival according to multivariate Cox regression. Multivariate analysis identified TPD52L2 as an independent prognostic factor. Diagnostic Receiver Operating Characteristic (ROC) curves yielded area under the curve values of 0.813 (TPD52) and 0.807 (TPD52L2). The results of functional experiments suggested that TPD52/TPD52L2 knockdown inhibited proliferation, migration, G0/G1 arrest, and induced apoptosis. Mechanistically, TPD52/TPD52L2 silencing suppressed PI3K/Akt serine/threonine kinase (AKT)/mammalian target of rapamycin (mTOR) signaling and epithelial-mesenchymal transition marker expression.</p><p><strong>Conclusion: </strong>TPD52 and TPD52L2 are promising prognostic biomarkers in GC, with TPD52L2 exhibiting greater clinical relevance. Targeting these proteins may disrupt oncogenic signaling pathways and enhance immunotherapy efficacy, warranting further investigation in clinical trials.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":"24 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Functional genomics in the era of cancer immunotherapy: challenges and clinical implications.","authors":"","doi":"10.1093/bfgp/elae053","DOIUrl":"https://doi.org/10.1093/bfgp/elae053","url":null,"abstract":"","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":"24 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DeepMEns: an ensemble model for predicting sgRNA on-target activity based on multiple features.","authors":"Shumei Ding, Jia Zheng, Cangzhi Jia","doi":"10.1093/bfgp/elae043","DOIUrl":"10.1093/bfgp/elae043","url":null,"abstract":"<p><p>The CRISPR/Cas9 system developed from Streptococcus pyogenes (SpCas9) has high potential in gene editing. However, its successful application is hindered by the considerable variability in target efficiencies across different single guide RNAs (sgRNAs). Although several deep learning models have been created to predict sgRNA on-target activity, the intrinsic mechanisms of these models are difficult to explain, and there is still scope for improvement in prediction performance. To overcome these issues, we propose an ensemble interpretable model termed DeepMEns based on deep learning to predict sgRNA on-target activity. By using five different training and validation datasets, we constructed five sub-regressors, each comprising three parts. The first part uses one-hot encoding, wherein 0-1 representation of the secondary structure is used as the input to the convolutional neural network (CNN) with Transformer encoder. The second part uses the DNA shape feature matrix as the input to the CNN with Transformer encoder. The third part uses positional encoding feature matrices as the proposed input into a long short-term memory network with an attention mechanism. These three parts are concatenated through the flattened layer, and the final prediction result is the average of the five sub-regressors. Extensive benchmarking experiments indicated that DeepMEns achieved the highest Spearman correlation coefficient for 6 of 10 independent test datasets as compared to previous predictors, this finding confirmed that DeepMEns can accomplish state-of-the-art performance. Moreover, the ablation analysis also indicated that the ensemble strategy may improve the performance of the prediction model.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the impact of N4-acetylcytidine modification in RNA on non-neoplastic disease: unveiling its role in pathogenesis and therapeutic opportunities.","authors":"Keyu Wan, Tiantian Nie, Wenhao Ouyang, Yunjing Xiong, Jing Bian, Ying Huang, Li Ling, Zhenjun Huang, Xianhua Zhu","doi":"10.1093/bfgp/elae020","DOIUrl":"10.1093/bfgp/elae020","url":null,"abstract":"<p><p>RNA modifications include not only methylation modifications, such as m6A, but also acetylation modifications, which constitute a complex interaction involving \"writers,\" \"readers,\" and \"erasers\" that play crucial roles in growth, genetics, and disease. N4-acetylcytidine (ac4C) is an ancient and highly conserved RNA modification that plays a profound role in the pathogenesis of a wide range of diseases. This review provides insights into the functional impact of ac4C modifications in disease and introduces new perspectives for disease treatment. These studies provide important insights into the biological functions of post-transcriptional RNA modifications and their potential roles in disease mechanisms, offering new perspectives and strategies for disease treatment.</p>","PeriodicalId":55323,"journal":{"name":"Briefings in Functional Genomics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141263641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}