Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology最新文献

{"title":"Developing under artificial light is not so bright: Oxidative stress as a physiological response to light pollution across amphibian life stages","authors":"Tamara G. Petrović ,&nbsp;Jelena P. Gavrić-Čampar ,&nbsp;Pablo Burraco ,&nbsp;Branka R. Gavrilović ,&nbsp;Svetlana G. Despotović ,&nbsp;Tijana B. Radovanović ,&nbsp;Ana Radmilović ,&nbsp;Marko Mirč ,&nbsp;Nataša Tomašević Kolarov ,&nbsp;Marko D. Prokić","doi":"10.1016/j.cbpa.2025.111819","DOIUrl":"10.1016/j.cbpa.2025.111819","url":null,"abstract":"<div><div>Light pollution has emerged as a new environmental challenge. Natural light has long been a selecting force in shaping physiological, morphological and behavioral traits across species. The introduction of artificial light at night (ALAN) disrupts the natural light-dark cycle, affecting a broad range of biological processes. In amphibians, ALAN has been suggested as a potential driver behind demographic declines in recent years. In this study, we investigated how exposure to cold LED light affects the development, body length, and oxidative status of the European tree frog (<em>Hyla arborea</em>). Individuals were exposed to ALAN from egg hatching (GS25) to two developmental endpoints: the larvae (GS38) and the juveniles. The experiment included three night-time light conditions: a natural dark control (&lt;0.3 lx), LED light intensity at 20 lx, and LED light intensity at 90 lx. Larvae reared under artificial light reached metamorphosis in a shorter period and exhibited larger body sizes. Nocturnal light also induced changes in oxidative status across life stages. Tadpoles under both 20 lx and 90 lx levels experienced greater oxidative damage of lipids. Likewise, juveniles had higher oxidative damage of proteins and elevated activities of the first-line antioxidant defense enzymes (SOD, CAT and GSH-Px) in response to both light intensities. Our results show that exposure to ALAN shapes development and body size of amphibians, which appears to be accompanied by disturbances in redox balance. Overall, we highlight that ALAN can be a factor affecting amphibian populations, and thus it should be taken into consideration in future conservation strategies.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111819"},"PeriodicalIF":2.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sibling similarity in telomere length in Adélie penguin chicks","authors":"Coline Marciau ,&nbsp;Sophie Bestley ,&nbsp;David Costantini ,&nbsp;Olivia Hicks ,&nbsp;Mark Hindell ,&nbsp;Akiko Kato ,&nbsp;Thierry Raclot ,&nbsp;Cécile Ribout ,&nbsp;Yan Ropert-Coudert ,&nbsp;Frederic Angelier","doi":"10.1016/j.cbpa.2025.111818","DOIUrl":"10.1016/j.cbpa.2025.111818","url":null,"abstract":"<div><div>Early life telomere length is thought to influence and predict an individual's fitness. It has been shown to vary significantly in early life compared to adulthood. Investigating the factors influencing telomere length in young individuals is therefore of particular interest, especially as the relative importance of heredity compared to post-natal conditions remains largely uncertain. Adélie penguins are eco-indicators of the Antarctic ecosystem and their population are currently undergoing variable trajectories due to climate change. Here, we conducted a correlative study to investigate how telomere length was influenced by external and internal factors in Adélie penguin chicks. We found that most of the parameters we tested, including sex, body mass, brood size and hatching order as well as parental foraging trip duration, did not significantly influence chick telomere length at 32 days. However, siblings had similar telomere length, suggesting that hereditary factors play a stronger role in determining telomere length at this stage compared to the post-natal environment. In addition, telomere length and oxidative damage did not directly correlate but did interact in a complex way mediated by chick mass. High levels of oxidative damage were associated with longer telomeres in heavy chicks, whereas they were associated with shorter telomeres in light chicks. Although this mass-dependent relationship between telomere length and oxidative damage needs to be confirmed in future studies, it could reflect two different scenarios: (1) short telomeres may mimic the cost of poor nutritional conditions and oxidative damage in light chicks; (2) long telomeres may be maintained despite high oxidative damage in heavy chicks thanks to optimal nutritional conditions.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111818"},"PeriodicalIF":2.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential for heat shock exposure during early development to alter the molecular responses to subsequent exposure to heat stress in the rainbow trout","authors":"Rob J. McFarling ,&nbsp;Mickael Teixeira Alves ,&nbsp;Anke Lange ,&nbsp;Millie Clark ,&nbsp;Harry J. Woodrow ,&nbsp;Ioanna Katsiadaki ,&nbsp;Ronny van Aerle ,&nbsp;Eduarda M. Santos","doi":"10.1016/j.cbpa.2025.111817","DOIUrl":"10.1016/j.cbpa.2025.111817","url":null,"abstract":"<div><div>Fish are ectothermic animals with temperature playing a key role in their health, growth and survival. Greater occurrence of heat waves and temperature extremes, as a result of global climate change, has the potential to impact both wild and farmed populations. Within aquaculture, production is threatened by a multitude of stressors, including adverse temperatures. The propensity for environmental temperature during early embryo development to influence later life transcriptomic responses has been observed in numerous animal species, and, if harnessed, could provide a method for inducing phenotypic changes in adult aquaculture species. We hypothesise that exposure of rainbow trout embryos to temperature stress results in alterations to transcriptional responsiveness upon re-exposure later in life. To test this hypothesis, we exposed embryos to a range of different heat shock treatments during early development and then analysed their response to thermal stress at five days post hatch (dph), in comparison to naïve fish that experienced no early development heat shock. <em>Hsp70a</em> and <em>hsp70b</em> transcription was measured (using RT-QPCR) as a biomarker for thermal stress response. Significantly greater transcriptional induction of <em>hsp70a</em> in response to post-hatch thermal stress was found in heat shocked larvae compared to naïve larvae (<em>p</em> = 0.0085). The timing, intensity and duration of the initial heat shock was not found to statistically influence the alteration of <em>hsp</em> induction when compared to that of naïve larvae. Together, these results support our hypothesis that heat shock during early development has the potential to affect responsiveness to the same stressor later in life. Future studies should focus on understanding whether this could be utilised to increase robustness of fish in aquaculture.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111817"},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiomes are largely unchanged when exposed to their amphibian host's latitudinally variable upper thermal limit","authors":"Grace J. Vaziri ,&nbsp;Bryanna Caicedo ,&nbsp;Nicole Dahrouge ,&nbsp;William G. Ryerson ,&nbsp;Jon M. Davenport ,&nbsp;Maria Stager ,&nbsp;Korin R. Jones ,&nbsp;Carlin Frost ,&nbsp;Chad L. Seewagen ,&nbsp;Tracy A.G. Rittenhouse ,&nbsp;Daniel I. Bolnick","doi":"10.1016/j.cbpa.2025.111816","DOIUrl":"10.1016/j.cbpa.2025.111816","url":null,"abstract":"<div><div>Climate change will increase the frequency and severity of temperature extremes. Links between host thermal physiology and their gut microbiota suggest that organisms' responses to future climates may be mediated by their microbiomes, raising the question of how the thermal environment influences the microbiome itself. Vertebrate gut microbiomes influence the physiological plasticity of their hosts via effects on immunity, metabolism, and nutrient uptake. The gut microbiota of ectothermic vertebrates in particular are responsive to long-term, sub-lethal gradual increases in environmental temperature. Whether and how the gut microbiota respond to brief exposure to temperatures at the upper limit of host physiological tolerance (CT_max) is poorly understood but could have downstream effects on host fitness. We assayed the CT_max of wood frogs (<em>Lithobates sylvaticus</em>) from 15 populations across a 10° latitudinal gradient. We then characterized the gut microbiota of juveniles at two time points following exposure to CT_max. Frogs from higher latitudes had lower thermal tolerance (lower CT_max) than those from lower latitudes. Unexpectedly, exposure to upper survivable temperature had little to no detectable effect on the frogs' microbiota richness, stability, or composition. Instead, we found a strong effect of time in which frogs kept in recovery conditions for four days had less diverse, but more stable gut microbiota than those that had recovered for only one day, regardless of CT_max exposure. We conclude that while wood frogs from higher latitudes have reduced thermal tolerances than those from lower latitudes, their microbial communities are largely unaffected by brief exposure to high temperatures at the edge of their physiological limits.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111816"},"PeriodicalIF":2.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of severity and region of gill tissue resection on regeneration in Atlantic salmon (Salmo salar)","authors":"Ensiyeh Ghanizadeh-Kazerouni ,&nbsp;Dean J. Yoo ,&nbsp;Simon R.M. Jones ,&nbsp;Colin J. Brauner","doi":"10.1016/j.cbpa.2025.111815","DOIUrl":"10.1016/j.cbpa.2025.111815","url":null,"abstract":"<div><div>In a previous study, we demonstrated successful regeneration of Atlantic salmon gill tissue following up to 50 % filament resection. The present study explored 1) the capacity of gill tissue to regenerate following more severe trauma, 2) if regeneration potential varies across regions of the arch, and 3) how tissue loss impacts the physiology of neighboring unresected filaments. Fish were divided between two resected groups and a control non-resected one. In resection group-1, fish underwent 50 % and 75 % resection in the ventral and medial-dorsal regions of the first arch, while in resection group-2, the location of resection levels was reversed. The degree of filament regeneration and physiology of unresected filaments were measured at 4, 12 and 20 weeks-post-resection (WPR). Overall, the degree of regeneration was significantly higher in 50 % resected filaments relative to 75 % resected filaments. The degree of regeneration did not differ significantly between the resected groups for either of resection levels, suggesting negligible impact of filament location on arch on regeneration. The concentration of oxidized glutathione (GSSG), total glutathione (GSH), and citrate synthase activity (CSA) in intact filaments were comparable between resected and control fish at both 4 and 20 WPR. However, GSH concentration varied among resected fish with those exhibited higher GSH in intact filaments showed lower regeneration of 50 % resected filaments at 20 WPR. Our results indicate that gill tissue loss exceeding 50 % may significantly impair regeneration and that this level of tissue loss is not associated with a compensatory response (e.g. GSSG, GSH, CSA) of neighboring gill tissue.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111815"},"PeriodicalIF":2.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and lipid metabolic differences between migratory and Egyptian-domesticated Mallard ducks (Anas platyrhynchos)","authors":"Basma Sheta ,&nbsp;Asmaa Hassan ,&nbsp;Alaa El-Din Sallam ,&nbsp;Lotfy Habbak ,&nbsp;Ayman Hyder","doi":"10.1016/j.cbpa.2025.111814","DOIUrl":"10.1016/j.cbpa.2025.111814","url":null,"abstract":"<div><div>Although a giant Egyptian domestic non-migratory duck breed is phenotypically identical to the migratory Mallard, yet it is three times larger. The current study sought to determine the genetic and metabolic differences between this duck and Mallard, which arrives in Egypt in September for wintering and departs in March. Mitochondrial DNA control region (D-loop) was extracted, amplified, sequenced, and analyzed in both ducks. Both ducks were given a high-fat diet (HFD) for 6 weeks to assess their metabolic response to this diet. Polymorphism results indicated that the D-loop is highly variable and both populations expansion is balanced. The hierarchical analysis of molecular variants (AMOVA) and interpopulation difference parameters revealed significant genetic differentiation and minimal gene flow between migrant and resident populations. Phylogeny and Network analyses revealed that domestic ducks are a distinct group that separated from mallards. Physiologically, domestic duck blood and adipose tissue had a higher level of triglycerides and adipocyte volume than that of the depleting arriving migratory Mallard ducks, while leaving Mallard parameters were the highest, suggesting a high level of preparatory fat deposition and utilization before starting the trip. In response to HFD, the expression of FA uptake genes <em>cd36</em>, <em>fabp1</em> was upregulated similarly in livers of domestic and migratory Mallard ducks, while the expression of lipid accumulation genes <em>dgat2</em> and <em>plin2</em> was higher in domestic than in migratory Mallards. However, the highest body mass and adipocytes volume gain was observed in the arriving migratory Mallards. In pectoral muscle, the expression of <em>cd36</em> and <em>fabp3</em> was higher in domestic than in leaving ducks, while in arriving Mallards, both genes were not upregulated in response to HFD. <em>Dgat2</em> was upregulated only in domestic muscle, while lipid oxidation genes <em>cpt1, lpl</em>, and the controlling <em>ppara</em> were more upregulated in leaving Mallard. In conclusion, both ducks can be genetically and metabolically differentiated. Migratory mallards are more flexible and efficient in lipid metabolism than domestic ducks.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111814"},"PeriodicalIF":2.1,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food intake and the HPT axis in the cichlid fish: The implications of the gut-brain peptide cholecystokinin","authors":"Mallikarjun Gouda,&nbsp;C.B. Ganesh","doi":"10.1016/j.cbpa.2025.111813","DOIUrl":"10.1016/j.cbpa.2025.111813","url":null,"abstract":"<div><div>This work aimed to investigate the response of cholecystokinin (CCK) to starvation and its impact on food intake and the reproductive axis of the tilapia <em>Oreochromis mossambicus</em>. The fish subjected to 21 days of starvation showed a significant decrease in CCK immunoreactivity in the hypothalamus, pituitary gland, and intestine. The administration of injections of 0.5 and 5 μg of sulfated CCK-8 (CCK-8S) over 21 days resulted in a significant, dose-dependent decrease in food consumption. Administration of a high dose of CCK-8S (5 μg) caused a substantial decrease in the number of various spermatogenic cells, the intensity of androgen receptor immunoreactivity in the testis, and the percentage area of GnRH-immunoreactive fibres in the pars distalis of the pituitary gland (PPD), concurrent with a significant decline in serum levels of luteinizing hormone (LH) and 11-ketotestosterone (11-KT). Moreover, CCK-8S treatment markedly reduced the <em>in vitro</em> testicular 11-KT level. The findings indicate for the first time that CCK influences hypothalamic GnRH release into the pituitary gland, resulting in the inhibition of LH release from the pituitary and affecting testicular spermatogenesis, androgen receptor protein expression, and steroidogenesis in teleosts. In addition, CCK may also directly affect testicular steroidogenesis.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111813"},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of olfactory-related neuropeptide transcripts in male Macrobrachium rosenbergii in correlation to pheromone perception from molting females","authors":"Thanapong Kruangkum ,&nbsp;Kornchanok Jaiboon ,&nbsp;Rapeepun Vanichviriyakit ,&nbsp;Prasert Sobhon ,&nbsp;Charoonroj Chotwiwatthanakun","doi":"10.1016/j.cbpa.2025.111812","DOIUrl":"10.1016/j.cbpa.2025.111812","url":null,"abstract":"<div><div>Our previous studies revealed a mating attractant or possibly a pheromone released from molting reproductive mature female prawns, <em>Macrobrachium rosenbergii</em>, stimulates the expression of insulin-like androgenic gland hormones in a co-culture system. The released attractant is perceived by olfactory receptors with setae located on the short lateral antennules (slAn), which connect to the olfactory neuropil in the central nervous system (CNS) of male prawns. This neural signaling propagating through the CNS is mediated by at least four neuropeptides, namely neuropeptide F (NPF), short NPF (sNPF), tachykinin (TK), and allatostatin-A (ATS-A) whose transcripts have been detected in the present study. These deduced sequences, along with their conserved domains, serve as signatures of the identified neuropeptides, which were then compared with those found in other crustaceans and insects, whose nucleotide sequences were obtained from the nucleotide database. RT-PCR identified the expressions of the transcripts encoding these neuropeptides in the CNS. <em>In situ</em> hybridization specifically localized these transcripts in olfactory-associated neurons of cluster 9/11 of the deutocerebrum. Quantitative real-time PCR was used to quantify the expressions of the transcripts in response to the female attractants under different co-culture conditions: males with molting females (G1), males with intermolt females (G2), and slAn ablated males with molting females (G3). The transcripts were significantly increased on days 4–8 in the brain (Br) of males in G1 but not in G2 and G3. This suggests that expressions of the transcripts encoding the neuropeptides are associated with the perception of female mating pheromones through the slAn. This study is the first to show that female mating chemicals regulate the expressions and abundance of the olfactory neuropeptides, thus providing valuable insights for manipulation of mating of this species in aquaculture production.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111812"},"PeriodicalIF":2.1,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory and hematological physiology of day 15 chicken embryos (Gallus gallus domesticus) during water submergence and air recovery: Implications for bird embryos experiencing nest inundation","authors":"Benjamin Dubansky ,&nbsp;Maria Rojas Antich ,&nbsp;Hiroshi Tazawa ,&nbsp;Warren Burggren","doi":"10.1016/j.cbpa.2024.111797","DOIUrl":"10.1016/j.cbpa.2024.111797","url":null,"abstract":"<div><div>Bird nests of coastal or inland breeding birds can temporarily flood during high tides or storms. However, respiratory physiological disruption of such water submersion and implications for post-submergence survival are poorly understood. We hypothesized that respiratory physiological disturbances caused by submersion would be rapidly corrected following return to normal gas exchange across the eggshell, thus explaining survival of nest inundation in the field. We further hypothesized that the chicken embryo prior to hatching will develop the ability to recover from acid-base disturbance. We exposed day 15 embryonated chicken eggs (a well-studied point 3/4 through development) to half- or full submersion in water (producing moderate and severe hypoxia, respectively) or in mineral oil (anoxia) for periods of 2–24 h to create varying degrees of submersion-related respiratory and acid-base disturbances. Egg submergence was followed by up to six hours in air to determine the extent and rate of physiological recovery. Arterialized blood PO₂ and [lactate], acid-base and hematology were measured at frequent intervals (5 min to 2 h depending on tested variable) both during submersion and air recovery. Submersion in mineral oil – eliminating all gas exchange - proved lethal at two hours. Yet, calculated embryonic oxygen stores suggest submerged embryos should be able to maintain pre-submergence oxygen consumption for only ∼15 mins, suggesting a possibly adaptive immediate decline in metabolism upon submergence. Half- or full submergence in water created blood acid-base disturbances within as little as 5 min, with partial recovery towards the end of 24 h of submergence. Six hours of subsequent air recovery fully restored acid-base homeostasis. Hematological changes that appeared within 5 min of submersion - primarily red blood cell swelling - were eliminated within 1 h following return to air. Importantly, these data indicate a surprising resilience of the chicken embryo to temporary elimination of normal gas exchange which, if evident in other species, provides underlying mechanisms for surviving nest inundation.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111797"},"PeriodicalIF":2.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish models to explore epigenetic determinants of hypoxia-tolerance","authors":"William Johnston,&nbsp;Sally Adil,&nbsp;Catherine Cao,&nbsp;Niepukolie Nipu,&nbsp;Jan A. Mennigen","doi":"10.1016/j.cbpa.2025.111811","DOIUrl":"10.1016/j.cbpa.2025.111811","url":null,"abstract":"<div><div>The occurrence of environmental hypoxia in freshwater and marine aquatic systems has increased over the last century and is predicted to further increase with climate change. As members of the largest extant vertebrate group, freshwater fishes, and to a much lesser extent marine fishes, are vulnerable to increased occurrence of hypoxia. This is important as fishes render important ecosystem services and have important cultural and economic roles. Evolutionarily successful, fishes have adapted to diverse aquatic freshwater and marine habitats with different oxygen conditions. While some fishes exhibit genetic adaptions to tolerate hypoxia and even anoxia, others are limited to oxygen-rich habitats. Recent advances in molecular epigenetics have shown that some epigenetic machinery, especially histone- and DNA demethylases, is directly dependent on oxygen and modulates important transcription-regulating epigenetic marks in the process. At the post-transcriptional level, hypoxia has been shown to affect non-coding microRNA abundance. Together, this evidence adds a new molecular epigenetic basis to study hypoxia tolerance in fishes. Here, we review the documented and predicted changes in environmental hypoxia in aquatic systems and discuss the diversity and comparative physiology of hypoxia tolerance in fishes, including molecular and physiological adaptations. We then discuss how recent mechanistic advances in environmental epigenetics can inform future work probing the role of oxygen-dependent epigenetic marks in shaping organismal hypoxia-tolerance in fishes with a focus on within- and between-species variation, acclimation, inter- and multigenerational plasticity, and multiple climate-change stressors. We conclude by describing the translational potential of this approach for conservation physiology, ecotoxicology, and aquaculture.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"302 ","pages":"Article 111811"},"PeriodicalIF":2.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}