Critical Reviews in Immunology最新文献_第4页

Increased PKN2 and M2-polarized macrophages promote HCT116 cell invasion PKN2 和 M2 极化巨噬细胞的增加促进了 HCT116 细胞的侵袭

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2023052095

Cheng He, Yimei Lin, Feng Qiu, Qingxin Zeng

{"title":"Increased PKN2 and M2-polarized macrophages promote HCT116 cell invasion","authors":"Cheng He, Yimei Lin, Feng Qiu, Qingxin Zeng","doi":"10.1615/critrevimmunol.2023052095","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2023052095","url":null,"abstract":"Backgroud: Colorectal cancer is the third most common malignant tumor, with highly invasive and metastatic potential in the later stage. This study investigated the role of PKN2 overexpression and M2-polarized macrophages in dictating the malignant phenotype of colorectal cancer cells.\u0000Methods: HCT116 colorectal cancer cell line with PKN2 overexpression was generated to investigate the functional role of PKN2. THP-1 cells were polarized into M2-like macrophages, and the co-culture system of THP-1/M2 cells and HCT116 cells was established to examine the impacts of M2-polairzed macrophages on the malignant phenotype of colorectal cancer cells.\u0000Results: PKN2 overexpression promoted cell proliferation, migration and invasion in HCT116 colorectal cancer cells, and reduced spontaneous cell death in the cell culture. Besides, the presence of M2-polarized THP-1 cells significantly enhanced the aggressive phenotype of HCT116 cells. Both PKN2 overexpression and M2-polarized THP-1 cells increased the expression of NF-κB p65 in HCT116 cells, indicating that enhanced NF-κB signaling may contribute to the augmented aggressiveness of HCT116 cells.\u0000Conclusion: These findings suggest PKN2 as an oncogenic factor in colorectal cancer and that M2-polarized THP-1 cells may promote the progression of colorectal cancer by activating NF-κB signaling.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"21 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139101795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastric Cancer Immune Subtypes and Prognostic Modeling: Insights from Aging-Related Gene Analysis 胃癌免疫亚型和预后模型：衰老相关基因分析的启示

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024052391

Jian Shen, Minzhe Li

{"title":"Gastric Cancer Immune Subtypes and Prognostic Modeling: Insights from Aging-Related Gene Analysis","authors":"Jian Shen, Minzhe Li","doi":"10.1615/critrevimmunol.2024052391","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024052391","url":null,"abstract":"Gastric cancer (GC) is highly heterogeneous and influenced by aging-related factors. This study aimed to improve individualized prognostic assessment of GC by identifying aging-related genes and subtypes. Immune scores of GC samples from GEO and TCGA databases were calculated using ESTIMATE and scored as high immune (IS_high) and low immune (IS_low). ssGSEA was used to analyze immune cell infiltration. Univariate Cox regression was employed to identify prognosis-related genes. LASSO regression analysis was used to construct a prognostic model. GSVA enrichment analysis was applied to determine pathways. CCK-8, wound healing, and Transwell assays tested the proliferation, migration, and invasion of the GC cell line (AGS). Cell cycle and aging were examined using flow cytometry, β-galactosidase staining, and Western blotting. Two aging-related GC subtypes were identified. Subtype 2 was characterized as lower survival probability and higher risk, along with a more immune-responsive tumor microenvironment. Three genes (IGFBP5, BCL11B, and AKR1B1) screened from aging-related genes were used to establish a prognosis model. The AUC values of the model were greater than 0.669, exhibiting strong prognostic value. In vitro, IGFBP5 overexpression in AGS cells was found to decrease viability, migration, and invasion, alter the cell cycle, and increase aging biomarkers (SA-β-galactosidase, p53, and p21). This analysis uncovered the immune characteristics of two subtypes and aging-related prognosis genes in GC. The prognostic model established for three aging-related genes (IGFBP5, BCL11B, and AKR1B1) demonstrated good prognosis performance, providing a foundation for personalized treatment strategies aimed at GC.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"232 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA let-7c-5p Alleviates in Hepatocellular Carcinoma by Targeting Enhancer of Zeste Homolog 2: An study intersecting bioinformatic analysis and validated experiments 通过靶向泽斯特同源物增强子 2（Enhancer of Zeste Homolog 2）缓解肝细胞癌的微小核糖核酸（MicroRNA）let-7c-5p：一项生物信息学分析与验证实验交叉的研究

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024051519

Tianyu Lin, Xinli Guo, Qian Du, Wei Liu, Xin Zhong, Suihan Wang, Liping Cao

{"title":"MicroRNA let-7c-5p Alleviates in Hepatocellular Carcinoma by Targeting Enhancer of Zeste Homolog 2: An study intersecting bioinformatic analysis and validated experiments","authors":"Tianyu Lin, Xinli Guo, Qian Du, Wei Liu, Xin Zhong, Suihan Wang, Liping Cao","doi":"10.1615/critrevimmunol.2024051519","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051519","url":null,"abstract":"Objectives. Enhancer of zeste homolog 2 (EZH2) gene has a prognostic role in hepatocellular carcinoma (HCC). This study aimed to identify the role of microRNAs (miRNAs) let-7c-5p by targeting EZH2 in HCC.\u0000Methods. We downloaded gene and miRNA RNA-seq data from The Cancer Genome Atlas (TCGA) database. Differences in EZH2 expression between different groups were analyzed and the association of EZH2 expression with HCC prognosis was detected using Cox regression analysis. The miRNA-EZH2-pathway network was constructed. Dual-luciferase reporter assay was performed to detect the hsa-let-7c-5p-EZH2. Cell proliferation, migration, invasion, and apoptosis were detected by CCK-8, Wound healing, Transwell, and Flow cytometry, respectively. RT-qPCR and Western blot were used to detect the expression of let-7c-5p and EZH2.\u0000Results. EZH2 was upregulated in HCC tumors (P < 0.0001). Cox regression analysis showed that TCGA HCC patients with high EZH2 expression levels showed a short survival time (HR = 1.677, 95% CI 1.316-2.137; P < 0.0001). Seven miRNAs were negatively correlated with EZH2 expression and were significantly downregulated in HCC tumor samples (P < 0.0001), in which hsa-let-7c-5p was associated with prognosis in HCC (HR = 0.849 95% CI 0.739-0.975; P = 0.021). We identified 14 immune cells that showed significant differences in EZH2 high- and low- expression groups. Additionally, let-7c-5p inhibited HCC cell proliferation, migration, and invasion and reversed the promoted effects of EZH2 on HCC cell malignant characteristics.\u0000Conclusions. hsa-let-7c-5p-EZH2 significantly suppressed HCC malignant characteristics, which can be used for HCC prognosis.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"23 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139101858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of Diagnostic Markers Associated with Inflammation in Chronic Kidney Disease Based on WGCNA and Machine Learning 基于 WGCNA 和机器学习的慢性肾病炎症相关诊断标记物探索

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024051277

Qianjia Wu, Yang Yang, Chongze Lin

{"title":"Exploration of Diagnostic Markers Associated with Inflammation in Chronic Kidney Disease Based on WGCNA and Machine Learning","authors":"Qianjia Wu, Yang Yang, Chongze Lin","doi":"10.1615/critrevimmunol.2024051277","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051277","url":null,"abstract":"Chronic kidney disease (CKD) is a common disorder related to inflammatory pathways; its effective management remains limited. This study aimed to use bioinformatics analysis to find diagnostic markers that might be therapeutic targets for CKD. CKD microarray datasets were screened from the GEO database and the differentially expressed genes (DEGs) in CKD dataset GSE98603 were analyzed. Gene set variation analysis (GSVA) was used to explore the activity scores of the inflammatory pathways and samples. Algorithms such as weighted gene co-expression network analysis (WGCNA) and Lasso were used to screen CKD diagnostic markers related to inflammation. Then functional enrichment analysis of inflammation-related DEGs was performed. ROC curves were conducted to examine the diagnostic value of inflammation-related hub-genes. Lastly, quantitative real-time PCR further verified the prediction of bioinformatics. A total of 71 inflammation-related DEGs were obtained, of which 5 were hub genes. Enrichment analysis showed that these genes were significantly enriched in inflammation-related pathways (NF-κB, JAK-STAT, and MAPK signaling pathways). ROC curves showed that the 5 CKD diagnostic markers (TIGD7, ACTA2, ACTG2, MAP4K4, and HOXA11) also exhibited good diagnostic value. In addition, TIGD7, ACTA2, ACTG2, and HOXA11 expression was downregulated while MAP4K4 expression was upregulated in LPS-induced HK-2 cells. The present study identified TIGD7, ACTA2, ACTG2, MAP4K4, and HOXA11 as reliable CKD diagnostic markers, thereby providing a basis for further understanding of CKD in clinical treatments.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"19 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139918676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of key ubiquitination-related genes and their associated with immune infiltration in osteoarthritis based on mRNA-miRNA network 基于 mRNA-miRNA 网络的泛素化相关关键基因及其与骨关节炎免疫浸润的关联鉴定

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024051440

Dalu Yuan, Hailiang Shen, Lina Bai, Menglin Li, Qiujie Ye

{"title":"Identification of key ubiquitination-related genes and their associated with immune infiltration in osteoarthritis based on mRNA-miRNA network","authors":"Dalu Yuan, Hailiang Shen, Lina Bai, Menglin Li, Qiujie Ye","doi":"10.1615/critrevimmunol.2024051440","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051440","url":null,"abstract":"Objective: Osteoarthritis (OA) is a prevalent degenerative joint disease that is closely associated with functions of ubiquitination and immune cells, yet the mechanism remains ambiguous. This study aimed to find core ubiquitination-related genes and their correlative immune infiltration in OA using weighted gene co-expression network analysis (WGCNA).\u0000Methods: The ubiquitination-related genes, dataset GSE55235 and GSE143514 were obtained from open databases. WGCNA got used to investigate key co-expressed genes. Then, we screened differentially expressed miRNAs by “limma” package in R, and constructed mRNA-miRNA network. We conducted function enrichment analysis on the identified genes. CIBERSORT was then utilized to analysis the relevance between immune infiltration and genes. Lastly, RT-qPCR was further verifying the prediction of bioinformatics.\u0000Results: A sum of 144 ubiquitination-related genes in OA were acquired. Enrichment analysis indicated that obtained genes obviously involved in mTOR pathway to regulate the OA development. GRB2 and SEH1L and L-arginine synergistically regulate the mTOR signaling pathway in OA. Moreover, GRB2 and SEH1L were remarkably bound up with immune cell infiltration. Additionally, GRB2 expression was upregulated and SEH1L level was downregulated in the OA development by RT-qPCR experiment.\u0000Conclusion: The present study identified GRB2 and SEH1L as key ubiquitination-related genes which were involved in immune infiltration in OA patients, thereby providing new drug targets for OA.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"33 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139517053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Insights into Tanshinone IIA in the Amelioration of Post-Thyroidectomy Hypoparathyroidism 丹参酮 IIA 在改善甲状腺切除术后甲状旁腺功能减退症方面的机理研究

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024052462

Xiaoyu Qian, Lin Li, Liang Chen, Chao Shen, Jian Tang

{"title":"Mechanistic Insights into Tanshinone IIA in the Amelioration of Post-Thyroidectomy Hypoparathyroidism","authors":"Xiaoyu Qian, Lin Li, Liang Chen, Chao Shen, Jian Tang","doi":"10.1615/critrevimmunol.2024052462","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024052462","url":null,"abstract":"Background: Thyroidectomy causes impaired blood supply to the parathyroid glands, which leads to hypoparathyroidism. Tanshinone IIA (Tan IIA) is helpful in blood activation and cardiovascular protection. Therefore, the efficacy of Tan IIA in improving hypoparathyroidism was explored in this study. \u0000Methods: New Zealand white rabbits were utilized to establish a unilateral parathyroid gland ischemia injury model. The model was created by selectively ligating the main blood supply vessel of one parathyroid gland, and the rabbits were then divided into three groups receiving 1, 5, and 10 mg/kg of Tan IIA. Serum calcium and parathyroid hormone (PTH) levels were measured using specialized assay kits. Immunohistochemistry was used to assess the microvessel density (MVD) in parathyroid glands. Western blotting (WB) was used to analyze protein expression related to the PI3K/AKT signaling pathway and the pathway-associated HIF-1α and VEGF. Moreover, MMP-2 and MMP-9 involved in angiogenesis were detected by WB. \u0000Results: Tan IIA treatment effectively restored serum calcium and PTH levels in a dose-dependent manner. Notably, MVD in the parathyroid glands increased significantly, especially at higher doses. The Tan IIA treatment also elevated the p-PI3K/PI3K and p-AKT/AKT ratios, indicating that the PI3K/AKT pathway was reactivated. Moreover, Tan IIA significantly restored the decreased expression levels of VEGF and HIF-1α caused by parathyroid surgery. Additionally, Tan IIA increased MMP-2 and MMP-9 levels. \u0000Conclusion: Tan IIA activates the PI3K/AKT pathway, promotes angiogenesis by modulating VEGF, HIF-1α, MMP-2, and MMP-9, thereby further enhancing MVD within the parathyroid glands. This study demonstrates that Tan IIA improved post-thyroidectomy hypoparathyroidism.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"55 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic and Prognostic Value of Peripheral Neutrophil CD64 Index in Elderly Patients with Community-Acquired Pneumonia 社区获得性肺炎老年患者外周中性粒细胞 CD64 指数的诊断和预后价值

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024050769

Yan Li, Jing Zhang, Suhang Wang, Jie Cao

{"title":"Diagnostic and Prognostic Value of Peripheral Neutrophil CD64 Index in Elderly Patients with Community-Acquired Pneumonia","authors":"Yan Li, Jing Zhang, Suhang Wang, Jie Cao","doi":"10.1615/critrevimmunol.2024050769","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024050769","url":null,"abstract":"Community-acquired pneumonia (CAP) is a leading cause of hospitalization and mortality in the elderly. The peripheral blood neutrophil CD64 (nCD64) index is increasingly recognized for its association with poor pneumonia prognosis. A comprehensive investigation involving 128 elderly patients diagnosed with CAP, including 96 with non-severe CAP and 32 with severe CAP, from January 2020 to January 2021 was performed. The nCD64 index, CD4+, CD8+, C-reactive protein (CRP), white blood cell (WBC) count, procalcitonin (PCT), neutrophil (NEUT), and B lymphocyte count were determined using flow cytometry. Our findings reveal that patients with severe CAP exhibited significantly higher levels of nCD64 index, NEUT, WBC, CRP, and PCT. Intriguingly, lower CRP, nCD64 index, CURB-65 score, and PCT were associated with a higher survival rate. Notably, the nCD64 index demonstrated remarkable predictive efficiency for 28-d survival in CAP patients [area under the curve (AUC) = 0.907], surpassing other markers and even showing enhanced predictive power when combined with the CURB-65 score (AUC = 0.905). Furthermore, a negative association was observed between the nCD64 index and both CD4+, CD4+/CD8+ ratios, and B lymphocytes, highlighting its potential role in immune dysregulation. These findings underscore the critical importance of the nCD64 index in the early diagnosis, risk stratification, and prognostic evaluation of infections and immune responses in elderly CAP patients.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"302 2 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum interleukin 6 and ferritin levels are the independent risk factors for pneumonia in elderly patients 血清白细胞介素 6 和铁蛋白水平是老年患者肺炎的独立风险因素

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024051340

Hao Yuan, Jing Tian, Lu Wen

{"title":"Serum interleukin 6 and ferritin levels are the independent risk factors for pneumonia in elderly patients","authors":"Hao Yuan, Jing Tian, Lu Wen","doi":"10.1615/critrevimmunol.2024051340","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051340","url":null,"abstract":"Pneumonia is a common infection in elderly patients. We explored the correlations of serum interleukin-6 (IL-6) and ferritin (SF) levels with immune function/disease severity in elderly pneumonia patients. Study subjects included 151 pneumonia/58 healthy geriatrics, with their age/sex/body mass index (BMI)/disease course and severity/blood pressure/C-reactive protein (CRP)/procalcitonin (PCT)/smoking status documented. The disease severity was evaluated by pneumonia severity index (PSI). T helper 17 (Th17)/regulatory T (Treg) cell ratios and IL-6/SF/immunoglobulin G (IgG)/Th17 cytokine (IL-21)/Treg cytokine (IL-10) levels were assessed using flow cytometry/ELISA. The correlations between these indexes/independent risk factors in elderly patients with severe pneumonia were evaluated by Spearman/multivariate logistic regression analyses. Patients showed obvious differences in smoking and CRP/PCT levels. Pneumonia patients exhibited up-regulated Th17 cell ratio and serum IL-6/SF/IL-21/IL-10/IgG levels, down-regulated Treg cell ratio, and more evident differences were noted in severe cases. Serum IL-6/SF levels were positively correlated with disease severity, immune function, and IL-21/IL-10/IgG levels. Smoking history and IL-6/SF levels were identified as independent risk factors in severe pneumonia geriatrics. Collectively, serum IL-6 and SF levels in elderly pneumonia patients were conspicuously positively correlated with disease severity and IL-21/IL-10/IgG levels. CRP, PCT, IL-6, and SF levels were independent risk factors for severe pneumonia in elderly patients.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"4 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139646859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different doses of dexmedetomidine on the prevention of postoperative sleep disturbance and serum neurotransmitter level in patients under general anesthesia 不同剂量右美托咪定对预防全身麻醉患者术后睡眠障碍和血清神经递质水平的影响

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024051294

Huifei Lu, Fei He, Ying Huang, Zhongliang Wei

{"title":"Effects of different doses of dexmedetomidine on the prevention of postoperative sleep disturbance and serum neurotransmitter level in patients under general anesthesia","authors":"Huifei Lu, Fei He, Ying Huang, Zhongliang Wei","doi":"10.1615/critrevimmunol.2024051294","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024051294","url":null,"abstract":"Postoperative sleep disturbance is a common issue that affects recovery in patients undergoing general anesthesia. Dexmedetomidine (Dex) has a potential role in improving postoperative sleep quality. Thus, we evaluated the effects of different doses of Dex on postoperative sleep disturbance and serum neurotransmitters in patients undergoing radical gastrectomy under general anesthesia. Patients were assigned to the control, NS, and Dex (Dex-L/M/H) groups based on different treatment doses [0.2, 0.4, 0.6 μg/(kg·h)]. The Athens Insomnia Scale (AIS) and ELISA kits were used to assess sleep disturbance and serum neurotransmitter (GABA, 5-HT, NE) levels before surgery and on postoperative days 1/4/7. The effects of different doses of Dex on postoperative sleep disturbance incidence and serum neurotransmitter levels were analyzed by Fisher exact test, one-way and repeated measures ANOVA. Patients had no differences in gender, age, body mass index, operation time, and bleeding volume. Different doses of Dex reduced the postoperative AIS score of patients under general anesthesia, improved their sleep, and increased serum levels of 5-HT, NE, and GABA. Furthermore, the effects were dose-dependent within the range of safe clinical use doses. Specifically, Dex at doses of 0.2, 0.4, 0.6 μg/(kg·h) reduced postoperative AIS score, elevated serum neurotransmitter levels, and reduced postoperative sleep disturbance incidence. Collectively, Dex has a potential preventive effect on postoperative sleep disturbance in patients undergoing general anesthesia for radical gastrectomy. The optimal dose of Dex is between 0.2 and 0.6 μg/(kg·h), which significantly reduces the incidence of pos","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"167 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139562237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Metabolism-Related Prognostic Biomarkers and Immune Features of Head and Neck Squamous Cell Carcinoma 头颈部鳞状细胞癌代谢相关预后生物标志物和免疫特征的鉴定

IF 1.3 4区医学

Critical Reviews in Immunology Pub Date : 2024-01-01 DOI: 10.1615/critrevimmunol.2024050754

Rongjin Zhou, Junguo Wang

{"title":"Identification of Metabolism-Related Prognostic Biomarkers and Immune Features of Head and Neck Squamous Cell Carcinoma","authors":"Rongjin Zhou, Junguo Wang","doi":"10.1615/critrevimmunol.2024050754","DOIUrl":"https://doi.org/10.1615/critrevimmunol.2024050754","url":null,"abstract":"We aimed to identify an effective metabolic subtype and risk score to predict survival and immunotherapy response in head and neck squamous cell carcinoma (HNSCC). Data were obtained from an online database. We screened significant prognostic metabolism-related genes between the normal and tumor groups using a series of bioinformatics methods. Based on the selected prognostic genes, we conducted a subtype analysis to identify significantly different subtypes in HNSCC. We then investigated survival, immune features, and hallmark differences among different subtypes. LASSO was utilized to identify optimal genes for the risk score model construction. Finally, distribution of the risk score samples was analyzed for different subtypes. A total of 32 significantly prognostic metabolism-related genes were screened, and all samples were grouped into two subtypes: cluster 1 and cluster 2. Cluster 1 had worse survival. Different immune cell infiltration (CD8 T cells, macrophages, and regulatory T cells) and immune checkpoint gene expression (PD-1 and CLAT-4) were observed between the two clusters. Twelve optimal genes were involved in risk score model, and high-risk group had poorer survival. Cluster 1 contained more high-risk samples (60%). Finally, four genes CAV1, GGT6, PYGL, and HS3ST1 were identified as significantly related to immune cells, and these genes were differentially expressed in the normal oral epithelial cells and HNSCC cells. The subtypes and risk score model in the study provide a promising biomarker for prognosis and immunotherapy response.","PeriodicalId":55205,"journal":{"name":"Critical Reviews in Immunology","volume":"40 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139461538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0