Cytoskeleton最新文献_第7页

Picture of the month by Samriddha Ray, Chamika DeSilva, Natasha Cruz-Calderon, and Daniel McCollum 本月图片由 Samriddha Ray、Chamika DeSilva、Natasha Cruz-Calderon 和 Daniel McCollum 拍摄。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-10-20 DOI: 10.1002/cm.21930

引用次数: 0

Myosin VI Is Associated With the Endoplasmic Reticulum in Regions of Sertoli Cells Containing Tubulobulbar Complexes 肌球蛋白 VI 与内质网相关联，位于含有管胞复合体的 Sertoli 细胞区域。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-10-10 DOI: 10.1002/cm.21949

Samuel Tretjakov, Prunveer Palia, A. Wayne Vogl

{"title":"Myosin VI Is Associated With the Endoplasmic Reticulum in Regions of Sertoli Cells Containing Tubulobulbar Complexes","authors":"Samuel Tretjakov, Prunveer Palia, A. Wayne Vogl","doi":"10.1002/cm.21949","DOIUrl":"10.1002/cm.21949","url":null,"abstract":"Myosin VI has been reported by others to localize in association with various regions of apical tubulobulbar complexes (TBCs) at sites of attachment between Sertoli cells and late spermatids in the mouse. Tubulobulbar complexes internalize “intact” intercellular junctions during sperm release and during spermatocyte translocation through the blood-testis barrier. Here, we use super-resolution (STED—stimulated emission depletion) and electron microscopy of immunolabeled sections of rat testis to clearly define the localization of anti-myosin VI reactivity both at apical and basal sites in the epithelium. In data stacks collected by STED imaging, staining at TBCs was predominantly associated with bulb regions of the complexes. At apical sites, when data stacks were analyzed with an Imaris software, staining appeared around and extended between adjacent bulbs. At basal sites, in addition to labeling at TBC bulbs, reactive sites appeared concentrated in regions close to but not directly associated with intercellular junctions. At the ultrastructural level, labeling was predominantly associated with cisternae of the endoplasmic reticulum associated with the bulbs of TBCs and near to basal junction complexes. We conclude that myosin VI may be associated with specific subdomains of the endoplasmic reticulum related to TBC bulbs and associated basal junction complexes between Sertoli cells.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 6","pages":"333-343"},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remembrance of Robert S. Adelstein: Mr. Nonmuscle Myosin 2 纪念罗伯特-阿德斯坦（Robert S. Adelstein）先生：非肌肉肌球蛋白 2 先生

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-10-10 DOI: 10.1002/cm.21948

James R. Sellers

引用次数: 0

Author Profile: Paul Griffin 作者简介：保罗-格里芬

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-10-07 DOI: 10.1002/cm.21919

Paul J. Griffin

引用次数: 0

Remembrance of Edward D. Korn: A Pioneer in Our Field 纪念爱德华-科恩（Edward D. Korn）：我们领域的先驱

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-10-01 DOI: 10.1002/cm.21942

John A. Hammer

引用次数: 0

Multifaceted role of the actin-binding protein WIP: Promotor and inhibitor of tumor progression and dissemination 肌动蛋白结合蛋白 WIP 的多方面作用：肿瘤进展和扩散的促进因子和抑制因子

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-09-27 DOI: 10.1002/cm.21935

Jorge Alonso-Eiras, Ines M. Anton

{"title":"Multifaceted role of the actin-binding protein WIP: Promotor and inhibitor of tumor progression and dissemination","authors":"Jorge Alonso-Eiras, Ines M. Anton","doi":"10.1002/cm.21935","DOIUrl":"10.1002/cm.21935","url":null,"abstract":"Cancer cells depend on actin cytoskeleton reorganization to achieve hallmark malignant functions including abnormal activation, proliferation, migration and invasiveness. (Neural)-Wiskott–Aldrich Syndrome protein ((N-)WASP) binds actin and forms a complex with the WASP-interacting protein (WIP), which plays a critical role in regulating the actin cytoskeleton, through (N)-WASP-dependent and independent functions. Mutations in the WIP gene (WIPF1) lead to severe early onset immunodeficiency in humans and severe autoimmunity and shortened lifespan in mice. This review covers the available evidence about the physiological role of WIP in different tissues and its contribution to human disease, focusing on cancer. In solid tumors overexpression of WIP has mostly been associated with tumor initiation, progression and dissemination through matrix degradation by invadopodia, while a suppressive function has been shown for WIP in certain hematological cancers. Interestingly, a minority of studies suggest a protective role for WIP in specific tumor contexts. These data support the need for further research to fully understand the mechanisms underlying WIP's diverse functions in health and disease and raise important questions for future work.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 3","pages":"186-196"},"PeriodicalIF":2.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21935","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Picture of the month by Aparna Bhattacharyya and Kenneth Barbee 阿帕纳-巴塔查里亚（Aparna Bhattacharyya）和肯尼斯-巴比（Kenneth Barbee）拍摄的本月图片。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-09-25 DOI: 10.1002/cm.21932

引用次数: 0

Tristetraprolin affects invasion-associated genes expression and cell motility in triple-negative breast cancer model Tristetraprolin可影响三阴性乳腺癌模型中侵袭相关基因的表达和细胞运动。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-09-25 DOI: 10.1002/cm.21934

Anastasiia Hubiernatorova, Josef Novak, Michaela Vaskovicova, David Sekac, Serhii Kropyvko, Zdenek Hodny

{"title":"Tristetraprolin affects invasion-associated genes expression and cell motility in triple-negative breast cancer model","authors":"Anastasiia Hubiernatorova, Josef Novak, Michaela Vaskovicova, David Sekac, Serhii Kropyvko, Zdenek Hodny","doi":"10.1002/cm.21934","DOIUrl":"10.1002/cm.21934","url":null,"abstract":"Tristetraprolin (TTP) is an RNA-binding protein that negatively regulates its target mRNAs and has been shown to inhibit tumor progression and invasion. Tumor invasion requires precise regulation of cytoskeletal components, and dysregulation of cytoskeleton-associated genes can significantly alter cell motility and invasive capability. Several genes, including SH3PXD2A, SH3PXD2B, CTTN, WIPF1, and WASL, are crucial components of the cytoskeleton reorganization machinery and are essential for adequate cell motility. These genes are also involved in invasion processes, with SH3PXD2A, SH3PXD2B, WIPF1, and CTTN being key components of invadopodia—specialized structures that facilitate invasion. However, the regulation of these genes is not well understood. This study demonstrates that ectopic expression of TTP in MDA-MB-231 cells leads to decreased mRNA levels of CTTN and SH3PXD2A, as well as defects in cell motility and actin filament organization. Additionally, doxorubicin significantly increases TTP expression and reduces the mRNA levels of cytoskeleton-associated genes, enhancing our understanding of how doxorubicin may affect the transcriptional profile of cells. However, doxorubicin affects target mRNAs differently than TTP ectopic expression, suggesting it may not be the primary mechanism of doxorubicin in breast cancer (BC) treatment. High TTP expression is considered as a positive prognostic marker in multiple cancers, including BC. Given that doxorubicin is a commonly used drug for treating triple-negative BC, using TTP as a prognostic marker in this cohort of patients might be limited since it might be challenging to understand if high TTP expression occurred due to the favorable physiological state of the patient or as a consequence of treatment.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 5","pages":"311-326"},"PeriodicalIF":2.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21934","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct molecular features of FLNC mutations, associated with different clinical phenotypes FLNC 基因突变的不同分子特征与不同的临床表型有关。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-09-24 DOI: 10.1002/cm.21922

Klimenko E.S, Zaytseva A.K, Sorokina M.Yu, Perepelina K.I, Rodina N.L, Nikitina E.G, Sukhareva K.S, Khudiakov A.A, Vershinina T.L, Muravyev A.S, Mikhaylov E.N, Pervunina T.M, Vasichkina E.S, Kostareva A.A

{"title":"Distinct molecular features of FLNC mutations, associated with different clinical phenotypes","authors":"Klimenko E.S, Zaytseva A.K, Sorokina M.Yu, Perepelina K.I, Rodina N.L, Nikitina E.G, Sukhareva K.S, Khudiakov A.A, Vershinina T.L, Muravyev A.S, Mikhaylov E.N, Pervunina T.M, Vasichkina E.S, Kostareva A.A","doi":"10.1002/cm.21922","DOIUrl":"10.1002/cm.21922","url":null,"abstract":"Filamin С is a key an actin-binding protein of muscle cells playing a critical role in maintaining structural integrity and sarcomere organization. FLNC mutations contribute to various types of cardiomyopathies and myopathies through potentially different molecular mechanisms. Here, we described the impact of two clinically distinct FLNC variants (R1267Q associated with arrhythmogenic cardiomyopathy and V2264M associated with restrictive cardiomyopathy) on calcium homeostasis, electrophysiology, and gene expression profile of iPSC-derived patient-specific cardiomyocytes. We demonstrated that R1267Q FLNC variant leads to greater disturbances in calcium dynamics, Nav1.5 kinetics and action potentials compared to V2264M variant. These functional characteristics were accompanied by transcriptome changes in genes linked to action potential and sodium transport as well as structural cardiomyocyte genes. We suggest distinct molecular effects of two FLNC variants linked to different types of cardiomyopathies in terms of myofilament structure, electrophysiology, ion channel function and intracellular calcium homeostasis providing the molecular the bases for their different clinical phenotypes.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 3","pages":"158-174"},"PeriodicalIF":2.4,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

F-actin in the cuticular plate and junctions of auditory hair cells is regulated by ADF and cofilin to allow for normal stereocilia bundle patterning and maintenance 听觉毛细胞角质板和连接处的 F-肌动蛋白受 ADF 和 cofilin 的调控，以实现正常的立体纤毛束模式化和维持。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-09-21 DOI: 10.1002/cm.21933

Jamis McGrath, Katelin Hawbaker, Benjamin J. Perrin

{"title":"F-actin in the cuticular plate and junctions of auditory hair cells is regulated by ADF and cofilin to allow for normal stereocilia bundle patterning and maintenance","authors":"Jamis McGrath, Katelin Hawbaker, Benjamin J. Perrin","doi":"10.1002/cm.21933","DOIUrl":"10.1002/cm.21933","url":null,"abstract":"Auditory hair cells, which convert sound-induced vibrations in the inner ear into neural signals, depend on multiple actin populations for normal function. Stereocilia are mechanosensory protrusions formed around a core of linear, crosslinked F-actin. They are anchored in the cuticular plate, which predominantly consists of randomly oriented actin filaments. A third actin population is found near hair cell junctions, consisting of both parallel and branched filaments. Actin depolymerizing factor (ADF) and cofilin-1 (CFL1) proteins disassemble actin filaments and are required to regulate F-actin in stereocilia, but their effect on cuticular plate and junctional actin populations is unclear. Here, we show that loss of ADF and CFL1 disrupts the patterning of stereocilia into orderly bundles and that this phenotype correlates with defective development of the cuticular plate and junctional actin populations. ADF/CFL1 continue to regulate these actin populations in mature cells, which is necessary for long-term maintenance of hair cell morphology.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 5","pages":"302-310"},"PeriodicalIF":2.4,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0