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Collective effect of Vigna sp. (mung) tubulin GTP hydrolysis rate divergence on microtubule filament assembly Vigna sp. (mung)管蛋白 GTP 水解速率差异对微管丝组装的集体影响。
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-09-02 DOI: 10.1002/cm.21923
Jashaswi Basu, Chaitanya A. Athale
{"title":"Collective effect of Vigna sp. (mung) tubulin GTP hydrolysis rate divergence on microtubule filament assembly","authors":"Jashaswi Basu,&nbsp;Chaitanya A. Athale","doi":"10.1002/cm.21923","DOIUrl":"10.1002/cm.21923","url":null,"abstract":"<p>Microtubules (MTs) are dynamic cytoskeletal filaments with highly conserved sequences across evolution, polymerizing by the GTP-dependent assembly of tubulin subunits. Despite the sequence conservation, MT polymerization kinetics diverge quantitatively between vertebrate brain, the model plant <i>Arabidopsis</i> and the protozoan <i>Plasmodium</i>. Previously, tubulin purified from seedlings of the plant <i>Vigna</i> sp. (mung) by temperature cycling was found to have a very low critical concentration. However, the lengths of MTs were sub-micron, much shorter than brain tubulin filaments. This was explained in simulations to be the result of the collective effect of high nucleation and GTP hydrolysis rates. Here, we test the effect of GTPase rates of affinity-purified <i>Vigna</i> sp. tubulin on microtubule polymerization and elongation. Affinity-purified mung tubulin is active and has a critical concentration of .37 μM. The GTP-dependent polymerization kinetics are transient, consistent with previous results. Polymerization is stabilized in the presence of either GTP analog GMPPNP (non-hydrolyzable) or GMPCPP (slow-hydrolyzable). Using interference reflection microscopy (IRM) we find polymerization with the non-hydrolysable analog significantly increases filament numbers, while lengths are unaffected for both GTP analogs. However, prolonged incubation with slow-hydrolyzable GMPCPP results in long filaments, pointing to GTP hydrolysis as a key factor determining MT length. We find the average GTPase turnover number of mung tubulin is 22.8 min<sup>−1</sup>, compared to 2.04 min<sup>−1</sup> for goat brain tubulin. Thus modulating GTPase rates affects both nucleation and elongation. This quantitative divergence in kinetics despite high sequence conservation in the GTPase domains of <i>α</i>- and <i>β</i>-tubulin could help better understand the roles of selective pressure and function in the diverse organisms.</p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 4","pages":"242-259"},"PeriodicalIF":2.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author profile: Hongsheng Xue 作者简介:薛宏声
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-09-02 DOI: 10.1002/cm.21904
Hongsheng Xue
{"title":"Author profile: Hongsheng Xue","authors":"Hongsheng Xue","doi":"10.1002/cm.21904","DOIUrl":"10.1002/cm.21904","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"504"},"PeriodicalIF":2.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author profile: Amrita Kumari 作者简介:阿姆里塔-库马里
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-31 DOI: 10.1002/cm.21921
Amrita Kumari
{"title":"Author profile: Amrita Kumari","authors":"Amrita Kumari","doi":"10.1002/cm.21921","DOIUrl":"10.1002/cm.21921","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"697-698"},"PeriodicalIF":2.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author profile: Paula Nieto Morales 作者简介:保拉-涅托-莫拉莱斯
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-31 DOI: 10.1002/cm.21914
Paula Nieto Morales
{"title":"Author profile: Paula Nieto Morales","authors":"Paula Nieto Morales","doi":"10.1002/cm.21914","DOIUrl":"10.1002/cm.21914","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"913-914"},"PeriodicalIF":2.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author profile: Albin E. Berg 作者简介:阿尔宾-伯格
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-26 DOI: 10.1002/cm.21920
Albin E. Berg
{"title":"Author profile: Albin E. Berg","authors":"Albin E. Berg","doi":"10.1002/cm.21920","DOIUrl":"10.1002/cm.21920","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"917-918"},"PeriodicalIF":2.4,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Author profile: Brooke E. Waechtler 作者简介:布鲁克-E-韦希特勒
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-26 DOI: 10.1002/cm.21917
Brooke E. Waechtler
{"title":"Author profile: Brooke E. Waechtler","authors":"Brooke E. Waechtler","doi":"10.1002/cm.21917","DOIUrl":"10.1002/cm.21917","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"915-916"},"PeriodicalIF":2.4,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Front Cover Image 封面图片
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-18 DOI: 10.1002/cm.21906
{"title":"Front Cover Image","authors":"","doi":"10.1002/cm.21906","DOIUrl":"https://doi.org/10.1002/cm.21906","url":null,"abstract":"<p>ON THE FRONT COVER: Nematic organization of growing actin filaments generated using agent-based simulations.</p><p>Credit: Deb Sankar Banerjee (Carnegie Mellon University; The University of Chicago) and Shiladitya Banerjee (Carnegie Mellon University)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 8","pages":"C1"},"PeriodicalIF":2.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21906","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inner Back Cover Image 封底内页图片
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-18 DOI: 10.1002/cm.21908
{"title":"Inner Back Cover Image","authors":"","doi":"10.1002/cm.21908","DOIUrl":"https://doi.org/10.1002/cm.21908","url":null,"abstract":"<p>ON THE INNER BACK COVER: Short and finite microtubule tactoids in presence of anti-parallel crosslinker, MAP65. In absence of salt, microtubules form short tactoids like formations.</p><p>Credit: Prashali Chauhan and Jennifer Ross (Syracuse University)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 8","pages":"C3"},"PeriodicalIF":2.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21908","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Back Cover Image 封底图片
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-18 DOI: 10.1002/cm.21909
{"title":"Back Cover Image","authors":"","doi":"10.1002/cm.21909","DOIUrl":"https://doi.org/10.1002/cm.21909","url":null,"abstract":"<p>ON THE BACK COVER: Fasin-bundled actin networks encapsulated inside giant unilamellar vesicles</p><p>Credit: Nadab H. Wubshet (University of Michigan; Harvard University) and Allen Liu (University of Michigan)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 8","pages":"C4"},"PeriodicalIF":2.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inner Front Cover Image 封面内页图片
IF 2.4 4区 生物学
Cytoskeleton Pub Date : 2024-08-18 DOI: 10.1002/cm.21907
{"title":"Inner Front Cover Image","authors":"","doi":"10.1002/cm.21907","DOIUrl":"https://doi.org/10.1002/cm.21907","url":null,"abstract":"<p>ON THE INNER FRONT COVER: Long microtubule bundles. As we increase the salt, we observe long microtubule bundles.</p><p>Credit: Prashali Chauhan and Jennifer Ross (Syracuse University)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 8","pages":"C2"},"PeriodicalIF":2.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21907","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142002590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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