Cytoskeleton最新文献_第10页

Phosphorylation at the Helm: Kinase-Mediated Regulation of Primary Cilia Assembly and Disassembly 掌舵磷酸化：激酶介导的初级纤毛组装和拆卸的调控。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-03-10 DOI: 10.1002/cm.22012

Andrea Lacigová, Lukáš Čajánek

引用次数: 0

The Interplay Between Early Chondrocyte Spreading and Inflammatory Responsivity 早期软骨细胞扩散与炎症反应的相互作用。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-03-05 DOI: 10.1002/cm.22011

Tristan Isaiah Pepper, Saitheja Adi Pucha, Lauren Foster, Alan Y. Liu, Meghan Alexander, Jay Milan Patel

{"title":"The Interplay Between Early Chondrocyte Spreading and Inflammatory Responsivity","authors":"Tristan Isaiah Pepper, Saitheja Adi Pucha, Lauren Foster, Alan Y. Liu, Meghan Alexander, Jay Milan Patel","doi":"10.1002/cm.22011","DOIUrl":"10.1002/cm.22011","url":null,"abstract":"<div>\u0000 \u0000 <p>Joint injuries are increasingly common and initiate a degenerative cascade in the cartilage extracellular matrix. Chondrocytes experience both intra- and extra-cellular changes during the initial phases of this process, including inflammatory activation and morphological change, initiating a catabolic feedback cycle that progresses toward osteoarthritis (OA). However, the link between this early morphological spreading and susceptibility to future inflammatory events is unclear. Thus, the objective of this study was to explore the implications of cellular spreading on early inflammatory activation. First, we treated bovine cartilage explants with control or degenerative media for 2 weeks and established early chondrocyte spreading and extracellular matrix loss around chondrocytes. Next, we either seeded chondrocytes on or encapsulated them within gelatin hydrogels of different stiffnesses to allow different degrees of spreading, followed by a short (2 h) inflammatory stimulus to measure inflammatory activation (NF-κB). We found in 2D that stiffer substrates led to greater chondrocyte spreading and NF-κB nuclear localization; however, in 3D, this trend was reversed, with the greatest spreading and activation in cells in the softest hydrogels. Finally, we investigated how hyaluronic acid hydrogel incorporation into these environments could impact this spreading-inflammtory activation relationship, showing that augmentation with HA reduced both facets. In conclusion, chondrocyte spreading, especially in 3D, is linked with reduced matrix stiffness, and this can make chondrocytes more susceptible to inflammation. Thus, future therapies should seek to address not only the inflammation in the joint but also to restore chondrocyte morphology and microenvironmental properties.</p>\u0000 </div>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 9","pages":"592-600"},"PeriodicalIF":1.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin-Mediated Endocytosis 在网格蛋白介导的胞吞作用模型中，快速肌动蛋白分解和纤蛋白机械敏感性支持快速周转。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-03-04 DOI: 10.1002/cm.22002

Sayed Iman Mousavi, Michael M. Lacy, Xiaobai Li, Julien Berro

{"title":"Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin-Mediated Endocytosis","authors":"Sayed Iman Mousavi, Michael M. Lacy, Xiaobai Li, Julien Berro","doi":"10.1002/cm.22002","DOIUrl":"10.1002/cm.22002","url":null,"abstract":"<div>\u0000 \u0000 <p>The actin cytoskeleton is central to force production in numerous cellular processes in eukaryotic cells. During clathrin-mediated endocytosis (CME), a dynamic actin meshwork is required to deform the membrane against high membrane tension or turgor pressure. Previous experimental work from our lab showed that several endocytic proteins, including actin and actin-interacting proteins, turn over several times during the formation of a vesicle during CME in yeast, and their dwell time distributions were reminiscent of gamma distributions with a peak around 1 s. However, the distribution for the filament cross-linking protein fimbrin contains a second peak around 0.5 s. To better understand the nature of these dwell time distributions, we developed a stochastic model for the dynamics of actin and its binding partners. Our model demonstrates that very fast actin filament disassembly is necessary to reproduce experimental dwell time distributions. Our model also predicts that actin-binding proteins bind rapidly to nascent filaments and filaments are fully decorated. Last, our model predicts that fimbrin detachment from actin endocytic structures is mechanosensitive to explain the extra peak observed in the dwell time distribution.</p>\u0000 </div>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 10","pages":"653-668"},"PeriodicalIF":1.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143544796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Profile: Jashaswi Basu 作者简介：Jashaswi Basu。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2025-03-04 DOI: 10.1002/cm.22010

Jashaswi Basu

引用次数: 0

Author Profile: Klimenko Ekaterina Sergeevna 作者简介：Klimenko Ekaterina Sergeevna。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2025-02-27 DOI: 10.1002/cm.22008

Klimenko Ekaterina Sergeevna

引用次数: 0

Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin 肌动蛋白丝尖端：原调蛋白和原肌球蛋白对其组装和拆卸的调控。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-02-24 DOI: 10.1002/cm.22007

Sawako Yamashiro, Shashank Shekhar, Stefanie M. Novak, Sudipta Biswas, Carol C. Gregorio, Velia M. Fowler

{"title":"Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin","authors":"Sawako Yamashiro, Shashank Shekhar, Stefanie M. Novak, Sudipta Biswas, Carol C. Gregorio, Velia M. Fowler","doi":"10.1002/cm.22007","DOIUrl":"10.1002/cm.22007","url":null,"abstract":"<p>Actin filaments are dynamic polymers whose length depends on regulated monomer association and dissociation at their ends. Actin barbed-end dynamics are relatively better understood, primarily due to the approximately tenfold faster subunit on/off rates at barbed versus pointed ends. We present experimental approaches to selectively assay actin pointed-end regulation using bulk biochemistry, single filament imaging, and live cell microscopy with an emphasis on tropomodulins (Tmods), a conserved family of eukaryotic proteins that specifically cap pointed ends. Average pointed-end assembly/disassembly rates are measured in bulk solution using pyrene-labeled actin and barbed end-capping protein CapZ. Direct rate measurements of individual pointed ends are performed via microfluidic-assisted total internal reflection fluorescence microscopy (mf-TIRF). Actin pointed-end dynamics in living cells are examined in striated muscle cells expressing fluorescent actin, where the regular arrays of 1- to 2-μm-long actin filaments in sarcomeres enable visualization of filament pointed and barbed ends. These assays will also help advance our understanding of other pointed end regulators, including cyclase-associated protein and leiomodins, which have been implicated in filament stabilization, disassembly, and elongation. This work is relevant to the musculoskeletal field, where precise regulation of filament lengths is particularly critical for sarcomere organization and striated muscle contraction.</p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 9","pages":"571-591"},"PeriodicalIF":1.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12353072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological Inhibition of β Myosin II Disrupts Sarcomere Assembly in Human iPSC-Derived Cardiac Myocytes β肌球蛋白II的药理抑制破坏人ipsc来源的心肌细胞的肌节组装。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-02-17 DOI: 10.1002/cm.22006

James B. Hayes, Dylan T. Burnette

{"title":"Pharmacological Inhibition of β Myosin II Disrupts Sarcomere Assembly in Human iPSC-Derived Cardiac Myocytes","authors":"James B. Hayes, Dylan T. Burnette","doi":"10.1002/cm.22006","DOIUrl":"10.1002/cm.22006","url":null,"abstract":"<p>Sarcomeres are the fundamental contractile units of striated muscle. The functional roles of the cardiac-specific myosin heavy chains, MYH6 (α myosin II) and MYH7 (β myosin II) during sarcomere assembly remain controversial. To address this, we utilized a selective MYH7 inhibitor, mavacamten, in combination with siRNA-mediated knockdown of MYH6 or MYH7 in human induced pluripotent stem cell-derived cardiomyocytes (hiCMs). Our results demonstrate that sarcomere assembly proceeds when either MYH6 or MYH7 is independently depleted, suggesting functional redundancy. However, pharmacological inhibition of MYH7 contractility by mavacamten disrupts sarcomere assembly in a concentration-dependent manner. Sensitivity to mavacamten correlated with the relative abundance of MYH6 and MYH7: sarcomere assembly by MYH7-enriched (i.e., MYH6-depleted) hiCMs was more sensitive to mavacamten (IC<sub>50</sub> = 0.1 μM), while assembly by MYH6-enriched (i.e., MYH7-depleted) hiCMs was less sensitive (IC<sub>50</sub> = 0.5 μM). These findings suggest that MYH7-mediated contractility is required for sarcomere assembly, but only when MYH7 is present within a cardiac myocyte. We conclude that the MYH7/MYH6 ratio impacts the susceptibility of sarcomere assembly to pharmacological inhibition.</p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 12","pages":"795-803"},"PeriodicalIF":1.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRiC Is a Structural Component of Mammalian Sperm Axonemes TRiC是哺乳动物精子轴突的结构成分。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-02-10 DOI: 10.1002/cm.22005

Alan Brown, Miguel Ricardo Leung, Tzviya Zeev-Ben-Mordehai, Rui Zhang

引用次数: 0

Betrayal From the Core: Centriolar and Cytoskeletal Subversion by Infectious Pathogens 核心背叛：感染性病原体对中心粒和细胞骨架的颠覆。

IF 1.6 4区生物学

Cytoskeleton Pub Date : 2025-02-04 DOI: 10.1002/cm.22004

Himanshi Amita, Zidhan Subair, Tulasiram Mora, Pranay Eknath Dudhe, Karthigeyan Dhanasekaran

{"title":"Betrayal From the Core: Centriolar and Cytoskeletal Subversion by Infectious Pathogens","authors":"Himanshi Amita, Zidhan Subair, Tulasiram Mora, Pranay Eknath Dudhe, Karthigeyan Dhanasekaran","doi":"10.1002/cm.22004","DOIUrl":"10.1002/cm.22004","url":null,"abstract":"<p>Microbes and parasites have evolved several means to evade and usurp the host cellular machinery to mediate pathogenesis. Being the major microtubule-organizing center (MTOC) of the cell, the centrosome is targeted by multiple viral and nonviral pathogens to mediate their assembly and trafficking within the host cell. This review examines the consequence of such targeting to the centrosome and associated cytoskeletal machinery. We have also amassed a substantial body of evidence of viruses utilizing the cilia within airway epithelium to mediate infection and the hijacking of host cytoskeletal machinery for efficient entry, replication, and egress. While infections have been demonstrated to induce structural, functional, and numerical aberrations in centrosomes, and induce ciliary dysfunction, current literature increasingly supports the notion of a pro-viral role for these organelles. Although less explored, the impact of bacterial and parasitic pathogens on these structures has also been addressed very briefly. Mechanistically, the molecular pathways responsible for these effects remain largely uncharacterized in many instances. Future research focusing on the centriolar triad comprising the centrosome, cilia, and centriolar satellites will undoubtedly provide vital insights into the tactics employed by infectious agents to subvert the host centriole and cytoskeleton-based machinery.</p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 11","pages":"681-706"},"PeriodicalIF":1.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.22004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Picture of the Month by E. S. Klimenko 本月图片，E. S. Klimenko。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2025-02-04 DOI: 10.1002/cm.22001

引用次数: 0