Cytoskeleton最新文献_第9页

Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia FAP93 在衣藻纤毛近端轴丝膜上的组装。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-01-15 DOI: 10.1002/cm.21818

Juyeon Hwang, Haruaki Yanagisawa, Keira C. Davis, Emily L. Hunter, Laura A. Fox, Ariana R. Jimenez, Reagan E. Goodwin, Sarah A. Gordon, Courtney D. E. Stuart, Raqual Bower, Mary E. Porter, Susan K. Dutcher, Winfield S. Sale, Karl F. Lechtreck, Lea M. Alford

{"title":"Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia","authors":"Juyeon Hwang, Haruaki Yanagisawa, Keira C. Davis, Emily L. Hunter, Laura A. Fox, Ariana R. Jimenez, Reagan E. Goodwin, Sarah A. Gordon, Courtney D. E. Stuart, Raqual Bower, Mary E. Porter, Susan K. Dutcher, Winfield S. Sale, Karl F. Lechtreck, Lea M. Alford","doi":"10.1002/cm.21818","DOIUrl":"10.1002/cm.21818","url":null,"abstract":"To identify proteins specific to the proximal ciliary axoneme, we used iTRAQ to compare short (~2 μm) and full-length (~11 μm) axonemes of Chlamydomonas. Known components of the proximal axoneme such as minor dynein heavy chains and LF5 kinase as well as the ciliary tip proteins FAP256 (CEP104) and EB1 were enriched in short axonemes whereas proteins present along the length of the axoneme were of similar abundance in both samples. The iTRAQ analysis revealed that FAP93, a protein of unknown function, and protein phosphatase 2A (PP2A) are enriched in the short axonemes. Consistently, immunoblots show enrichment of FAP93 and PP2A in short axonemes and immunofluorescence confirms the localization of FAP93 and enrichment of PP2A at the proximal axoneme. Ciliary regeneration reveals that FAP93 assembles continuously but more slowly than other axonemal structures and terminates at 1.03 μm in steady-state axonemes. The length of FAP93 assembly correlates with ciliary length, demonstrating ciliary length-dependent assembly of FAP93. Dikaryon rescue experiments show that FAP93 can assemble independently of IFT transport. In addition, FRAP analysis of GFP-tagged FAP93 demonstrates that FAP93 is stably anchored in the axoneme. FAP93 may function as a scaffold for assembly of other specific proteins at the proximal axoneme.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"539-555"},"PeriodicalIF":2.4,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional and structural significance of the inner-arm-dynein subspecies d in ciliary motility 内臂-染料蛋白亚种 d 在睫状肌运动中的功能和结构意义。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2024-01-12 DOI: 10.1002/cm.21828

Ryosuke Yamamoto, Takahide Kon

{"title":"Functional and structural significance of the inner-arm-dynein subspecies d in ciliary motility","authors":"Ryosuke Yamamoto, Takahide Kon","doi":"10.1002/cm.21828","DOIUrl":"10.1002/cm.21828","url":null,"abstract":"Motile cilia play various important physiological roles in eukaryotic organisms including cell motility and fertility. Inside motile cilia, large motor-protein complexes called “ciliary dyneins” coordinate their activities and drive ciliary motility. The ciliary dyneins include the outer-arm dyneins, the double-headed inner-arm dynein (IDA f/I1), and several single-headed inner-arm dyneins (IDAs a, b, c, d, e, and g). Among these single-headed IDAs, one of the ciliary dyneins, IDA d, is of particular interest because of its unique properties and subunit composition. In addition, defects in this subspecies have recently been associated with several types of ciliopathies in humans, such as primary ciliary dyskinesia and multiple morphologic abnormalities of the flagellum. In this mini-review, we discuss the composition, structure, and motor properties of IDA d, which have been studied in the model organism Chlamydomonas reinhardtii, and further discuss the relationship between IDA d and human ciliopathies. In addition, we provide future perspectives and discuss remaining questions regarding this intriguing dynein subspecies.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"569-577"},"PeriodicalIF":2.4,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21828","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139426114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Titin takes centerstage among cytoskeletal contributions to myocardial passive stiffness 在细胞骨架对心肌被动僵化的贡献中，滴定蛋白占据中心位置

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-29 DOI: 10.1002/cm.21827

Christine M. Loescher, Wolfgang A. Linke

{"title":"Titin takes centerstage among cytoskeletal contributions to myocardial passive stiffness","authors":"Christine M. Loescher, Wolfgang A. Linke","doi":"10.1002/cm.21827","DOIUrl":"10.1002/cm.21827","url":null,"abstract":"Both diastolic filling and systolic pumping of the heart are dependent on the passive stiffness characteristics of various mechanical elements of myocardium. However, the specific contribution from each element, including the extracellular matrix, actin filaments, microtubules, desmin intermediate filaments, and sarcomeric titin springs, remains challenging to assess. Recently, a mouse model allowing for precise and acute cleavage of the titin springs was used to remove one mechanical element after the other from cardiac fibers and record the effect on passive stiffness. It became clear that the stiffness contribution from each element is context-dependent and varies depending on strain level and the force component considered (elastic or viscous); elements do not act in isolation but in a tensegral relationship. Titin is a substantial contributor under all conditions and dominates the elastic forces at both low and high strains. The contribution to viscous forces is more equally shared between microtubules, titin, and actin. However, the extracellular matrix substantially contributes to both force components at higher strain levels. Desmin filaments may bear low stiffness. These insights enhance our understanding of how different filament networks contribute to passive stiffness in the heart and offer new perspectives for targeting this stiffness in heart failure treatment.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 2-3","pages":"184-187"},"PeriodicalIF":2.9,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraflagellar transport: A critical player in photoreceptor development and the pathogenesis of retinal degenerative diseases 囊内转运：感光器发育和视网膜变性疾病发病机制中的关键角色。

IF 2.4 4区生物学

Cytoskeleton Pub Date : 2023-12-23 DOI: 10.1002/cm.21823

Mohona Gupta, Gregory J. Pazour

引用次数: 0

Tau and Alzheimer's disease: Past, present and future Tau 和阿尔茨海默病：过去、现在和未来。

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-21 DOI: 10.1002/cm.21822

Khalid Iqbal

{"title":"Tau and Alzheimer's disease: Past, present and future","authors":"Khalid Iqbal","doi":"10.1002/cm.21822","DOIUrl":"10.1002/cm.21822","url":null,"abstract":"My journey with tau started when in 1974 for the first time I isolated neurofibrillary tangles of paired helical filaments (PHFs) from autopsied Alzheimer's disease (AD) brains and discovered that they were made up of a ~50–70 KDa protein on SDS-polyacrylamide gels. Subsequently my team discovered that this PHF protein and the microtubule-associated factor called tau were one and the same protein. However, we found that tau in neurofibrillary tangles/PHFs in AD brain was abnormally hyperphosphorylated, and unlike normal tau, which promoted the assembly of tubulin into microtubules, the AD-hyperphosphorylated tau inhibited microtubule assembly. These discoveries of tau pathology in AD opened a new and a major area of research on tau and on the molecular pathology of this major cause of dementia in middle- and old-age individuals. Tau pathology, which without fail is made up of the aggregated hyperphosphorylated state of the protein, is also the hallmark lesion of a family of around 20 related neurodegenerative diseases, called tauopathies. Currently, tau pathology is a major drug target for the treatment of AD and related tauopathies. Both active and passive tau immunization human clinical trials at various stages are underway. Initial results range from negative to partially promising. Future studies will reveal whether tau therapy alone or in combination with drugs targeting Aβ and/or neurodegeneration will be required to achieve the most effective treatment for AD and related disorders.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 1","pages":"116-121"},"PeriodicalIF":2.9,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21822","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

‘10th cell architecture in development and disease (CADD)’: Meeting report 发育和疾病中的第 10 个细胞结构 (CADD)"：会议报告

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-16 DOI: 10.1002/cm.21815

Thomas Fath, Vladimir Sytnyk, Ramón Martínez-Mármol

引用次数: 0

Tau here, tau there, tau almost everywhere: Clarifying the distribution of tau in the adult CNS Tau 在这里，Tau 在那里，Tau 几乎无处不在：明确 tau 在成人中枢神经系统中的分布

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-16 DOI: 10.1002/cm.21820

Nicholas M. Kanaan

{"title":"Tau here, tau there, tau almost everywhere: Clarifying the distribution of tau in the adult CNS","authors":"Nicholas M. Kanaan","doi":"10.1002/cm.21820","DOIUrl":"10.1002/cm.21820","url":null,"abstract":"The microtubule-associated protein tau has gained significant attention over the last several decades primarily due to its apparent role in the pathogenesis of several diseases, most notably Alzheimer's disease. While the field has focused largely on tau's potential contributions to disease mechanisms, comparably less work has focused on normal tau physiology. Moreover, as the field has grown, some misconceptions and dogmas regarding normal tau physiology have become engrained in the traditional narrative. Here, one of the most common misconceptions regarding tau, namely its normal cellular/subcellular distribution in the CNS, is discussed. The literature describing the presence of tau in neuronal somata, dendrites, axons and synapses, as well as in glial cells is described. The origins for the erroneous description of tau as an “axon-specific,” “axon-enriched” and/or “neuron-specific” protein are discussed as well. The goal of this work is to help address these specific dogmatic misconceptions and provide a concise description of tau's normal cellular/subcellular localization in the adult CNS. This information can help refine our collective understanding of- and hypotheses about tau biology and pathobiology.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 1","pages":"107-115"},"PeriodicalIF":2.9,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21820","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138685549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Back Cover Image 封底图片

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-16 DOI: 10.1002/cm.21825

引用次数: 0

An interview with Douglas N. Robinson—Johns Hopkins School of Medicine, Baltimore, Maryland, USA 与美国马里兰州巴尔的摩市道格拉斯-罗宾逊-约翰斯-霍普金斯医学院的访谈。

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-16 DOI: 10.1002/cm.21817

Paul Trevorrow, Douglas N. Robinson

引用次数: 0

Front Cover Image 封面图片

IF 2.9 4区生物学

Cytoskeleton Pub Date : 2023-12-16 DOI: 10.1002/cm.21824

引用次数: 0