Cytoskeleton最新文献

{"title":"Inner Front Cover Image","authors":"","doi":"10.1002/cm.21966","DOIUrl":"https://doi.org/10.1002/cm.21966","url":null,"abstract":"<p>ON THE INNER FRONT COVER: A genetic mosaic C. elegans adult stained for myosin A and UNC-89 (vertebrate homolog is obscurin) in body-wall muscle shows disorganized UNC-89/obscurin in a muscle cell that does not express myosin A.</p><p>Credit: Sarah Almuhanna (Imam Abdulrahman Bin Faisal University College of Medicine).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C2"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21966","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Cover Image","authors":"","doi":"10.1002/cm.21968","DOIUrl":"https://doi.org/10.1002/cm.21968","url":null,"abstract":"<p>ON THE BACK COVER: Immunofluorescence analysis of a histological section derived from a cutaneous squamous cell carcinoma specimen. S100A4 (green), primarily expressed by fibroblast cells, cytokeratin 14 (red), primarily expressed by basal epidermal cells, and DAPI (blue) to mark cell nuclei.</p><p>Credit: Alexandra Pittar, Centre for Cancer Biology, an Alliance between SA Pathology and the University of South Australia, Adelaide, Australia.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C4"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inner Back Cover Image","authors":"","doi":"10.1002/cm.21967","DOIUrl":"https://doi.org/10.1002/cm.21967","url":null,"abstract":"<p>ON THE INNER BACK COVER: Immunofluorescence analysis of a histological section derived from a cutaneous squamous cell carcinoma specimen. S100A4 (green), primarily expressed by fibroblast cells, cytokeratin 14 (red), primarily expressed by basal epidermal cells, and DAPI (blue) to mark cell nuclei.</p><p>Credit: Alexandra Pittar, Centre for Cancer Biology, an Alliance between SA Pathology and the University of South Australia, Adelaide, Australia.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C3"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21967","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Cover Image","authors":"","doi":"10.1002/cm.21957","DOIUrl":"https://doi.org/10.1002/cm.21957","url":null,"abstract":"<p>ON THE FRONT COVER: Artistic representation of an expansion microscopy image of human kidney tubular epithelium. Tissues were immunostained for primary cilia (acetylated α-tubulin, yellow), cell-cell junctions (E-cadherin, green/blue), and nuclei (DAPI, dark grey). The cover image is based on the article “Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures”.</p><p>Credit: Ewa Langner and Moe Mahjoub (Department of Medicine, Washington University, St Louis, MO).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"C1"},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inner Back Cover Image","authors":"","doi":"10.1002/cm.21959","DOIUrl":"https://doi.org/10.1002/cm.21959","url":null,"abstract":"<p>ON THE INNER BACK COVER: Cross section of retinal photoreceptors. Green: outer segments (wheat germ agglutinin); red: actin filaments in the inner segment and outer segment base (phalloidin); blue: nuclei.</p><p>Credit for Mohona Gupta and Gregory Pazour (Program of Molecular Medicine, University of Massachusetts, Chan Medical School).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"C3"},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21959","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Cover Image","authors":"","doi":"10.1002/cm.21960","DOIUrl":"https://doi.org/10.1002/cm.21960","url":null,"abstract":"<p>ON THE BACK COVER: Waveforms predicted by a multifilament model of the axoneme for three different values of the elastic resistance to twisting, which may be attributed to torsional stiffness of radial spokes (increasing stiffness top to bottom).</p><p>Credit: Louis G. Woodhams and Philip V. Bayly (Washington University in St. Louis, McKelvey School of Engineering).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"C4"},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21960","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inner Front Cover Image","authors":"","doi":"10.1002/cm.21958","DOIUrl":"https://doi.org/10.1002/cm.21958","url":null,"abstract":"<p>ON THE INNER FRONT COVER: Expansion microscopy image of a mouse kidney tubular epithelium. Tissues were immunostained for primary cilia (acetylated α-tubulin, green), cell-cell junctions (E-cadherin, purple), cytoplasmic microtubules (α-tubulin, cyan), and nuclei (DAPI, orange). The cover image is based on the article “Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures”.</p><p>Credit: Ewa Langner (Department of Medicine, Washington University, St Louis, MO).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"C2"},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21958","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Picture of the Month by Giuliano Callaini","authors":"","doi":"10.1002/cm.21944","DOIUrl":"10.1002/cm.21944","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"699"},"PeriodicalIF":2.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyses of Off-Target Effects on Cardiac and Skeletal Muscles by Berberine, a Drug Used to Treat Cancers and Induce Weight Loss","authors":"Jushuo Wang,&nbsp;Yingli Fan,&nbsp;Syamalima Dube,&nbsp;Patricia Benz,&nbsp;Dipak Dube,&nbsp;Jean M. Sanger,&nbsp;Joseph W. Sanger","doi":"10.1002/cm.21950","DOIUrl":"10.1002/cm.21950","url":null,"abstract":"<div>\u0000 \u0000 <p>Previous reports from our laboratory describing the formation of myofibrils in cultured embryonic cardiac and skeletal muscle cells have proposed that myofibrillogenesis occurs in three steps of increasing protein organization: beginning with premyofibrils, followed by nascent myofibrils, and ending in mature myofibrils. Inhibitors of the ubiquitin proteasome system (UPS) prevented nascent myofibrils from progressing directly to mature myofibrils in cultured cardiac and skeletal muscle cells, supporting a three-step model of assembly in which some of the proteins in nascent myofibrils are proteolyzed to allow the assembly of mature myofibrils. Application of UPS inhibitors on cultured muscle cells suggests possible explanations for the off-target cardiac and skeletal muscle adverse effects of UPS drugs, which are used on cancer patients. Berberine, a plant derivative, has been used to treat various cancers, including multiple myelomas. In contrast to the use of UPS drugs, success was reported with Berberine in multiple myeloma patients with no off-target effects on their hearts. We have exposed cultured cardiac and skeletal muscle cells to Berberine, a ligase inhibitor of UHRF1 (ubiquitin-like with PHD and RING finger domains). Berberine inhibited myofibril assembly at the nascent myofibril stage in embryonic skeletal muscle cells but had no effect in the assembly of mature myofibrils in embryonic heart cells. RT-PCR experiments demonstrated Berberine inhibition of mRNA for muscle myosin II heavy chains but not for muscle actin mRNA in skeletal muscle cells. Berberine is also being used as a popular weight losing compound, because it is much cheaper and available without a prescription than the semaglutide containing weight losing drugs (Wegovy and Ozempic). In contrast to Berberine, semaglutide had no effects on myofibril assembly in culture assays for both cardiac and skeletal muscle cells. We postulate that analyses of cultured embryonic cardiac and skeletal muscle cells will provide a preclinical assay for the testing of novel cancer drugs with improved outcomes for patients, an important goal for cancer therapeutics.</p>\u0000 </div>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 6","pages":"344-359"},"PeriodicalIF":2.4,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SEC-SAXS/MC Ensemble Structural Studies of the Microtubule Binding Protein Cdt1 Show Monomeric, Folded-Over Conformations","authors":"Kyle P. Smith,&nbsp;Srinivas Chakravarthy,&nbsp;Amit Rahi,&nbsp;Manas Chakraborty,&nbsp;Kristen M. Vosberg,&nbsp;Marco Tonelli,&nbsp;Maximilian G. Plach,&nbsp;Arabela A. Grigorescu,&nbsp;Joseph E. Curtis,&nbsp;Dileep Varma","doi":"10.1002/cm.21954","DOIUrl":"10.1002/cm.21954","url":null,"abstract":"<p>Cdt1 is a mixed folded protein critical for DNA replication licensing and it also has a “moonlighting” role at the kinetochore via direct binding to microtubules and the Ndc80 complex. However, it is unknown how the structure and conformations of Cdt1 could allow it to participate in these multiple, unique sets of protein complexes. While robust methods exist to study entirely folded or unfolded proteins, structure–function studies of combined, mixed folded/disordered proteins remain challenging. In this work, we employ orthogonal biophysical and computational techniques to provide structural characterization of mitosis-competent human Cdt1. Thermal stability analyses shows that both folded winged helix domains1 are unstable. CD and NMR show that the N-terminal and linker regions are intrinsically disordered. DLS shows that Cdt1 is monomeric and polydisperse, while SEC-MALS confirms that it is monomeric at high concentrations, but without any apparent inter-molecular self-association. SEC-SAXS enabled computational modeling of the protein structures. Using the program SASSIE, we performed rigid body Monte Carlo simulations to generate a conformational ensemble of structures. We observe that neither fully extended nor extremely compact Cdt1 conformations are consistent with SAXS. The best-fit models have the N-terminal and linker disordered regions extended into the solution and the two folded domains close to each other in apparent “folded over” conformations. We hypothesize the best-fit Cdt1 conformations could be consistent with a function as a scaffold protein that may be sterically blocked without binding partners. Our study also provides a template for combining experimental and computational techniques to study mixed-folded proteins.</p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 6","pages":"372-387"},"PeriodicalIF":2.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12074537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}