CytoskeletonPub Date : 2024-12-09DOI: 10.1002/cm.21971
Bat-Ider Tumenbayar, Khanh Pham, John C. Biber, Vincent M. Tutino, Joseph A. Brazzo III, Peng Yao, Yongho Bae
{"title":"FAK and p130Cas Modulate Stiffness-Mediated Early Transcription and Cellular Metabolism","authors":"Bat-Ider Tumenbayar, Khanh Pham, John C. Biber, Vincent M. Tutino, Joseph A. Brazzo III, Peng Yao, Yongho Bae","doi":"10.1002/cm.21971","DOIUrl":"10.1002/cm.21971","url":null,"abstract":"<div>\u0000 \u0000 <p>Cellular metabolism is influenced by the stiffness of the extracellular matrix. Focal adhesion kinase (FAK) and its binding partner, p130Cas, transmit biomechanical signals, such as substrate stiffness, to the cell to regulate a variety of cellular responses, but their roles in early transcriptional and metabolic responses remain largely unexplored. We cultured mouse embryonic fibroblasts with or without siRNA-mediated FAK or p130Cas knockdown and assessed the early transcriptional responses of these cells to placement on soft and stiff substrates by RNA sequencing and bioinformatics analyses. Exposure to the stiff substrate altered the expression of genes important for metabolic and biosynthetic processes, and these responses were influenced by knockdown of FAK and p130Cas. Our findings reveal that FAK-p130Cas signaling mechanotransduces substrate stiffness to early transcriptional changes that alter cellular metabolism and biosynthesis.</p>\u0000 </div>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 3","pages":"197-215"},"PeriodicalIF":2.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CytoskeletonPub Date : 2024-12-04DOI: 10.1002/cm.21965
{"title":"Front Cover Image","authors":"","doi":"10.1002/cm.21965","DOIUrl":"https://doi.org/10.1002/cm.21965","url":null,"abstract":"<p>ON THE FRONT COVER: Myosin A staining in the earliest stages of muscle cytoskeletal organization in a comma-stage C. elegans embryos shows an anterior-posterior gradient in the organization of myosin A into a striated pattern.</p><p>Credit: Kar Men Lee and Hao Peng (Western Michigan University Department of Biological Sciences).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C1"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CytoskeletonPub Date : 2024-12-04DOI: 10.1002/cm.21966
{"title":"Inner Front Cover Image","authors":"","doi":"10.1002/cm.21966","DOIUrl":"https://doi.org/10.1002/cm.21966","url":null,"abstract":"<p>ON THE INNER FRONT COVER: A genetic mosaic C. elegans adult stained for myosin A and UNC-89 (vertebrate homolog is obscurin) in body-wall muscle shows disorganized UNC-89/obscurin in a muscle cell that does not express myosin A.</p><p>Credit: Sarah Almuhanna (Imam Abdulrahman Bin Faisal University College of Medicine).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C2"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21966","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CytoskeletonPub Date : 2024-12-04DOI: 10.1002/cm.21968
{"title":"Back Cover Image","authors":"","doi":"10.1002/cm.21968","DOIUrl":"https://doi.org/10.1002/cm.21968","url":null,"abstract":"<p>ON THE BACK COVER: Immunofluorescence analysis of a histological section derived from a cutaneous squamous cell carcinoma specimen. S100A4 (green), primarily expressed by fibroblast cells, cytokeratin 14 (red), primarily expressed by basal epidermal cells, and DAPI (blue) to mark cell nuclei.</p><p>Credit: Alexandra Pittar, Centre for Cancer Biology, an Alliance between SA Pathology and the University of South Australia, Adelaide, Australia.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C4"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21968","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CytoskeletonPub Date : 2024-12-04DOI: 10.1002/cm.21967
{"title":"Inner Back Cover Image","authors":"","doi":"10.1002/cm.21967","DOIUrl":"https://doi.org/10.1002/cm.21967","url":null,"abstract":"<p>ON THE INNER BACK COVER: Immunofluorescence analysis of a histological section derived from a cutaneous squamous cell carcinoma specimen. S100A4 (green), primarily expressed by fibroblast cells, cytokeratin 14 (red), primarily expressed by basal epidermal cells, and DAPI (blue) to mark cell nuclei.</p><p>Credit: Alexandra Pittar, Centre for Cancer Biology, an Alliance between SA Pathology and the University of South Australia, Adelaide, Australia.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"C3"},"PeriodicalIF":2.4,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21967","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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