Helminthologia最新文献

{"title":"When wildlife comes to town: interaction of sylvatic and domestic host animals in transmission of <i>Echinococcus</i> spp. in Namibia.","authors":"O Aschenborn,&nbsp;J Aschenborn,&nbsp;P Kern,&nbsp;U Mackenstedt,&nbsp;T Romig,&nbsp;M Wassermann","doi":"10.2478/helm-2023-0012","DOIUrl":"https://doi.org/10.2478/helm-2023-0012","url":null,"abstract":"<p><p>The present study was conducted in the isolated desert town of Oranjemund in the far south of Namibia. It is an extremely arid region where no livestock husbandry is practiced and only animals adapted to the desert can be found. However, in and around the city, artificial irrigation maintains lush green patches of grass that attract wild animals, in particular oryx antelopes (<i>Oryx gazella</i>). In 2015 four oryx antelopes were euthanised due to poor conditions and a post-mortem examination was conducted. Two were found positive for cystic echinococcosis and 16 cysts were collected for molecular analyses. In addition, faecal samples from black-backed jackals (n=5) and domestic dogs (n=9), which were regularly observed to feed on oryx carcasses, were collected and taeniid eggs isolated. Parasite species identification of the cysts and eggs was done by amplifying and sequencing the mitochondrial <i>nad1</i> gene. Both oryx antelopes were found infected with <i>E. ortleppi</i> and one co-infected with <i>E. canadensis</i> G6/7. Both <i>Echinococcus</i> species were able to develop fertile cysts in oryx, making oryx antelopes competent hosts for these parasites. Therefore, the analysis of faecal samples was of high interest and although the numbers were quite small, taeniid eggs were found in three out of five faecal samples of jackals and in all nine dog samples. However, species determination was only successful with two jackal and one dog sample. All three were positive for <i>E. canadensis</i> G6/7. The absence of <i>E. ortleppi</i> may be due to the low number of faecal samples examined. In our small study, we discovered a rather unique lifecycle of <i>Echinococcus</i> spp. between jackals and domestic dogs as definitive hosts and oryx antelopes as intermediate hosts. Here, the presence of <i>E. canadensis</i> G6/7 is of particular concern, as it is the second most important causative agent of CE in humans.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"117-124"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/81/b6/helm-60-2-helm-2023-0012.PMC10516471.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41141474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of flukes (<i>Fasciola hepatica</i> and paramphistomids) in cattle in south-eastern Mexico.","authors":"J C Hernández-Hernández,&nbsp;R González-Garduño,&nbsp;D O Ortiz-Pérez,&nbsp;A Villa-Mancera,&nbsp;M S Arias-Vázquez,&nbsp;A Paz-Silva","doi":"10.2478/helm-2023-0017","DOIUrl":"https://doi.org/10.2478/helm-2023-0017","url":null,"abstract":"<p><p>The objective of this study was to determine the risk factors and prevalence of trematodes in south-eastern Mexico. The prevalence of trematodes was determined in 1010 bovines. The study was carried out from October 2018 (n=291) to December 2019 (n=719). Only in 2019 rumen and liver fluke eggs were differentiated. Faecal samples (n=311) were obtained from farms in southeast Mexico located in Tabasco, Chiapas and Campeche. In addition, the presence of flukes in liver and rumen from slaughtered cattle in abattoirs was recorded with a total of 408 samples. A logistic procedure was used to obtain the prevalence and the effect of main risk factors such as land physiography (flooded areas and hills), year, sex, animals' age and type of sample obtained (eggs in faeces and flukes). The general prevalence of flukes in cattle was 32.3 % in 2018 and 41.7 % in 2019. Prevalence of <i>F. hepatica</i> (liver fluke) was 18.6 % (134/719) and that of paramphistomids (rumen fluke) was 33.4 % (240/719). The infected cattle from the slaughterhouse indicated a lower prevalence of <i>F. hepatica</i> (1 %) and rumen fluke (26.7 %) than in farms detected by egg in faeces (41.8 % and 42.1 %, respectively). The physiographic zone was decisive in the presence of <i>F. hepatica</i> and rumen fluke, while sex did not represent a risk factor (P > 0.05). The environmental conditions of the Mexican southeast favour the presence of both liver and rumen fluke.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"141-151"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/44/helm-60-2-helm-2023-0017.PMC10516476.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41172674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth fauna in roe deer (<i>Capreolus capreolus</i> Linnaeus, 1758) in the province of Grosseto (central Italy).","authors":"F Macchioni,&nbsp;F Vallone,&nbsp;C Lenzi,&nbsp;G Monni,&nbsp;A Matiacic,&nbsp;F Cecchi,&nbsp;G Romeo","doi":"10.2478/helm-2023-0013","DOIUrl":"https://doi.org/10.2478/helm-2023-0013","url":null,"abstract":"<p><p>Helminth infection was analysed at necropsy and coprology in a total of 54 roe deer from the province of Grosseto (central Italy) between 2018 - 2020. Age and sex data were recorded for each deer for a total of 31 adults (23 females, 8 males) and 23 juveniles (11 females, 12 males). The results on the small intestine (51 samples) highlighted that nematodes belonging to the species <i>Trichostrongylus colubriformis</i> were the most prevalent parasite (41.2 %), followed by the cestode <i>Moniezia expansa</i> (7.8 %). The large intestine results (52 samples) showed <i>Trichuris</i> spp. (53.8 %), <i>Oesophagostomum venulosum</i> (50 %) and <i>Chabertia ovina</i> (26.9 %). In the abomasum, only <i>Ostertagia ostertagi</i> (17.9 %) was found. Of the 34 samples analysed by bronchopulmonary, only the lung of an adult female was positive for <i>Dictyocaulus</i> spp. In two livers out of 33 samples analysed, nematodes of the species <i>Setaria tundra</i> were found on the surface. Copropositivity was observed in 45 of the 52 faecal samples analysed. The results of the present study indicate that the roe deer is host to several species of parasites, which are also common in other cervids and domestic ruminants. Statistical testing highlighted a significant difference between mean intensities in males and females.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"134-140"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/60/helm-60-2-helm-2023-0013.PMC10516473.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41153058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redescription and resurrection of the status of <i>Joyeuxiella gervaisi</i> () (Eucestoda, Dipylidiidae).","authors":"R K Schuster,&nbsp;N Mehmood,&nbsp;A Varcasia,&nbsp;V Veneziano","doi":"10.2478/helm-2023-0016","DOIUrl":"https://doi.org/10.2478/helm-2023-0016","url":null,"abstract":"<p><p>A study of the parasite fauna of feral cats in Dubai revealed the presence of two <i>Joyeuxiella</i> species, <i>J. pasqualei</i> (Diamare, 1893) and <i>J. fuhrmanni</i> (Baer, 1924). While the wide distribution of <i>J. pasqualei</i> includes countries of the Middle East, Africa, Asia and Europe, <i>J. fuhrmanni</i> was previously reported from felid hosts from southern Africa and has not been found in other cat parasite surveys in the Middle East, except from Dubai. The availability of historical references, however, raised doubts about the correctness of the allocation of the small <i>Joyeuxiella</i> sp. from Dubai cats to <i>J. fuhrmanni</i> and for this reason, a reexamination of stored material in the parasite collection of the Central Veterinary Research Laboratory in Dubai was carried out. A total of 40 specimens of the small <i>Joyeuxiella</i> sp. with a strobila length between 30 and 60 mm and consisting of 52 to 85 segments obtained from domestic cats and formerly allocated to <i>J. fuhrmanni</i> were studied. In complete specimens, 10 - 13 rows of rostellar hooks were counted. Mature segments were wider than long, round testes were concentrated posterior to coiled vasa deferentia and did not reach the anterior rim of the proglottids. Narrow cirri reached up to 520 μm in length. Gravid segments were longer than wide and egg capsules were restricted to the space between longitudinal excretory vessels. The examination revealed that the morphology of these cestodes matched the main characteristics of <i>J. fuhrmanni</i>. However, the little known cestode, <i>J. gervaisi</i> (Setti, 1895), that had been described from <i>Genetta abyssinica</i> imported from Eritrea 29 years earlier and was declared a <i>species inquirenda</i> met the same main morphological criteria. In this paper, the status of <i>J. gervaisi</i> as a valid species was resurrected and <i>J. fuhrmanni</i> was declared a junior synonym.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"166-174"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/a9/helm-60-2-helm-2023-0016.PMC10516474.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41156150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors associated with dog endoparasites infection spread in East Slovak Lowland.","authors":"L Ihnacik,&nbsp;J Šmigová,&nbsp;J Šoltys,&nbsp;P Blišťan,&nbsp;L Kovanič,&nbsp;M Blišťanová,&nbsp;I Schusterová,&nbsp;I Papajová","doi":"10.2478/helm-2023-0014","DOIUrl":"https://doi.org/10.2478/helm-2023-0014","url":null,"abstract":"<p><p>This study aimed to determine the prevalence of endoparasites in dog feces from public places, investigate the occurrence of endoparasites in soil, and identify potential risk factors associated with the dog endoparasites infection spread in East Slovak Lowland - the region near the EU border with Ukraine. In a one-year study, a total of 803 dog fecal samples and 148 soil samples from public places were examined for the presence of endoparasite developmental stages. In general, 43.59 % of dog excrements were positive. Six different species of intestinal parasites were detected: <i>Toxocara canis</i> (22.65 %), <i>Trichuris vulpis</i> (14.07 %), family Ancylostomatidae (10.09 %), <i>Capillaria aerophilla</i> (5.60 %), <i>Ascaris</i> spp. (1.49 %). Altogether, 52.03 % of soil samples were positive for at least one endoparasites species. The occurrence of parasitic eggs in the soil was as follows: <i>Ascaris</i> spp. (37.16 %), <i>Toxocara</i> spp. (29.05 %), <i>Trichuris</i> spp. (29.05 %) %), family Ancylostomatidae (2.03 %) and <i>Toxascaris leonina</i> (2.03 %). The occurrence of endoparasites between the towns and villages varied significantly. The distribution of endoparasites in dogs and soil was affected by the population density, the minority people living in the studied territory, the number of people living in poor hygienic conditions with limited access to drinking water and the usage of sewerage. Persisting endoparasitic contamination of the environment in East Slovak Lowland forms a reservoir with zoonotic potential representing public and environmental health problems.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"152-160"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/03/helm-60-2-helm-2023-0014.PMC10516475.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41123823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of <i>Haemonchus contortus</i> isolated from the small ruminants of south Gujarat, India.","authors":"B Das,&nbsp;N Kumar,&nbsp;J B Solanki,&nbsp;M M Jadav,&nbsp;I H Kalyani","doi":"10.2478/helm-2023-0020","DOIUrl":"https://doi.org/10.2478/helm-2023-0020","url":null,"abstract":"<p><p>The successful design of strategic control measures against the blood-sucking gastrointestinal nematode, <i>Haemonchus contortus</i> in small ruminants can be facilitated by revealing its general features from morphology to the molecular level. In the south Gujarat region of India, a total of 2408 <i>H. contortus</i> were collected from 84 slaughtered sheep's abomasum, consisting of 347 males and 2061 females (1:6 ratio) (p<0.05). Furthermore, 726 <i>H. contortus</i> were collected from 61 goats, comprising 145 males and 581 females (1:4 ratio) (p<0.05). The male worms were approximately 12±0.06 mm long, while female worms were about 20±0.09 mm long. The vulvar morphotypes of the female worms were found to be 17.7% linguiform, 76.6 % knobbed/button (p<0.05), and 5.7 % smooth type, demonstrating common features of <i>H. contortus</i>. The nucleotide sequences of the Internal Transcribed Spacer 1 (ITS-1) of 165 bp or ITS-2 plus of 256 bp were aligned, and it was found that the genotypes of male and female specimens of either sheep or goat origin were identical, with a 100 % match. The present isolates shared >95 % and >94 % homology with published sequences of ITS-1 and ITS-2 plus of <i>H. contortus</i>, respectively, with more nucleotide transitions than transversions in the aligned sequences. The reconstructed phylogram of either ITS-1 or ITS-2 plus revealed two major clades, one for <i>H. contortus</i> and another for other nematodes, with <i>Haemonchus placei</i> showing its proximity with the clade of <i>H. contortus</i>. The study established the role of morphological and molecular features in identifying and differentiating <i>H. contortus</i> parasite at the local level.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"175-188"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/bf/helm-60-2-helm-2023-0020.PMC10516478.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41161700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthelmintic effects of peppermint (<i>Mentha piperita</i>), lemon (<i>Citrus limon</i>), and tea tree (<i>Melaleuca alternifolia</i>) essential oils against Monogenean parasite (<i>Dactylogyrus</i> sp.) on carp (<i>Cyprinus carpio</i>).","authors":"B Harmansa Yilmaz,&nbsp;H Yavuzcan Yildiz","doi":"10.2478/helm-2023-0019","DOIUrl":"https://doi.org/10.2478/helm-2023-0019","url":null,"abstract":"<p><p><i>Dactylogyrus</i> sp. (Monogenea) is one of the most dangerous pathogens causing parasitic infections in carp (<i>Cyprinus carpio</i>) and other freshwater fish. Due to the adverse effects of conventional chemical treatments on the environment and fish, the use of herbal products in aquaculture against various diseases has increased. In the present study, anthelmintic effects of peppermint (<i>Mentha piperita</i>), lemon (<i>Citrus limon</i>), and tea tree (<i>Melaleuca alternifolia</i>) essential oils against <i>Dactylogyrus</i> sp. found on the gills of carp were studied using <i>in vitro</i> and <i>in vivo</i> experiments. In <i>in vitro</i> experiments, 1, 2.5, 5, and 10 μl/ml concentrations of these essential oils were tested on the parasites and cumulative mortalities were observed to reach 100 % after treatment with peppermint, lemon, and tea tree essential oils of 1 μl/ml concentration in approximately 15, 10 and 2 min, respectively. The <i>in vitro</i> experiments demonstrated that the cumulative mortality of the parasites increased with essential oil concentration and exposure duration. As the concentration of essential oil used increased, the observed mean time to death of parasites decreased. In <i>in vivo</i> experiments, median effective concentrations (EC50) as assessed by <i>in vitro</i> tests for peppermint and lemon essential oils in 5 min exposure and EC50 concentration for tea tree essential oil in 2 min exposure was applied on fish as a single bath and there was a significant decrease in the mean parasite intensities (p<0.05). Antiparasitic efficacies of peppermint, lemon, and tea tree essential oils were determined as 28.23 %, 30.95 %, and 35.31 %, respectively. The tea tree oil was the most effective and peppermint oil was the least effective in both <i>in vitro</i> and <i>in vivo</i> experiments. All three herbal essential oils tested in this study have weak antiparasitic potential against monogenean infections in fish.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"125-133"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/db/helm-60-2-helm-2023-0019.PMC10516477.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41107012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth Fauna of Juvenile Green Sea Turtles (<i>Chelonia mydas</i>) from Rio de Janeiro State, Brazil.","authors":"B Cavaco,&nbsp;L M Madeira de Carvalho,&nbsp;E Shimoda,&nbsp;M Santoro,&nbsp;M R Werneck","doi":"10.2478/helm-2023-0021","DOIUrl":"https://doi.org/10.2478/helm-2023-0021","url":null,"abstract":"<p><p>The helminth fauna of juvenile green sea turtles (<i>Chelonia mydas</i> Linnaeus, 1758) is still poorly known. Herein, we study the gastrointestinal helminths of 28 juvenile green sea turtles found stranded on the north coast of Rio de Janeiro state, Brazil. All turtles were infected showing a rich helminth fauna. In total, 14802 trematodes belonging to 30 species and 5 families including Micros-caphidiidae, Plagiorchiidae, Pronocephalidae, Hapalotrematidae, and Telorchiidae were recovered. An unidentified nematode specimens was also found. The mean intensity was 536 (95% CI = 362 - 853) (range: 1 - 2831), and the species richness was 7.86 (95% CI = 6.46 - 9.21) (range: 1 - 17). The coast of Rio de Janeiro state represents new locality records for <i>Angiodictyum posterovitellatum, Microscaphidium aberrans, M. warui, Octangium hyphalum, O. sagitta, Enodiotrema reductum</i> and <i>Pleurogonius laterouterus</i>. This study confirms that the green sea turtle harbors the richest helminth fauna among sea turtle species and provides useful information on the gastrointestinal helminths of a poorly known stage in the life cycle of this endangered chelonian.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 2","pages":"196-200"},"PeriodicalIF":1.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/e3/helm-60-2-helm-2023-0021.PMC10516470.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41121367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observations on the biology of <i>Postharmostomum ntowi</i> Hodasi, 1967 (Trematoda: Brachylaimidae) based on intermediate and definitive hosts found in Nigeria.","authors":"E O Alari, A P Ugbomeh, A O Awharitoma, E E Enabulele, M S O Aisien","doi":"10.2478/helm-2023-0006","DOIUrl":"10.2478/helm-2023-0006","url":null,"abstract":"<p><p>Following the recovery of the metacercariae of a brachylaimid trematode from the rectum of the frog <i>Amnirana galamensis</i> from Ase in Delta State, Nigeria, we investigated the land snails in the locality to establish their roles in the life cycle of the parasite. Of the four land snails investigated from Ase (<i>Limicolaria aurora</i>, <i>Archachatina marginata</i>, <i>A. papyracea</i>, and <i>Thapsia oscitans</i>), and a <i>Limicolaria</i> sp. from Tombia (Bayelsa State), four harboured larval stages of the bracylaimid. Only <i>L. aurora</i> and the <i>Limicolaria</i> sp. harboured cercariogenous sporocysts and are therefore presumed to serve as the first intermediate hosts of the parasite. Metacercariae were recovered from the <i>Limicolaria</i> spp. and the <i>Archachatina</i> spp. and so serve as the second intermediate hosts. No larval brachylaimids were recovered from <i>T. oscitans</i>. Metacercariae from <i>L. aurora</i> and <i>A. papyracea</i> were cultured <i>in vivo</i> in 14 days old chicks of <i>Gallus gallus domesticus</i>. Parasites recovered from the experimental hosts 7, 14, 21 and 28 days post-infection, showed progressive development of the parasite with the full maturity attained by the 28^th day post-infection. Adult parasites recovered from the experimental birds and from free range chicken purchased from Ase and Tombia showed that the brachylaimid infecting these birds was <i>Postharmostomum ntowi</i>, a parasite previously reported in domestic chicken in Ghana. There is need to investigate the host range of the parasite in Nigeria as this trematode is also known to infect the Guinea fowl in Ghana.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 1","pages":"84-93"},"PeriodicalIF":1.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5d/7e/helm-60-1-helm-2023-0006.PMC10251752.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of swine echinococcosis cytopathological tests and challenges for a differential diagnosis: slaughterhouse data.","authors":"A Toure, L Toure, G L Acapovi-Yao, C B V Senin, N Kone, M Kachani, E Couacy-Hymann","doi":"10.2478/helm-2023-0004","DOIUrl":"10.2478/helm-2023-0004","url":null,"abstract":"<p><p>Echinococcosis disease shows clinical signs similar to many diseases. Hence we report cases that need to be confirmed using appropriate tests. A confirmatory study has been conducted to assess the accuracy of two cytopathological tests, with the histopathology test as the reference standard. The first cytopathological test evaluates the Ziehl Neelsen staining with an epifluorescence microscope (cytopath 1). The second cytopathological test uses the same staining followed by a transmitted light microscope examination (cytopath 2). Of a total of 2524 inspected pigs, 101 suspected cases of echinococcosis were detected, of which 67 were found positive with the two cytopathological tests and the histopathological one. The specificity of cytopath 1 (100 % [95 % CI 100 - 100]) and cytopath 2 (100 % [95 % CI 100;100]) were similar, as well as their respective positive predictive values: 100 % [95 % CI 100 - 100] vs. 100 % [95 % CI 100 - 100]. The sensitivity of cytopath 1 is 79.66 % [95 % CI 69.39 - 89.93], while cytopath 2 equals 66.10 % [95 % CI 54.02 - 78.18]. The difference in sensitivity of both tests was not significant. Negative predictive values found for cytopath 1, and cytopath 2 were 40 [95 % CI 18.53 - 61.47] and 28.57 [95 % CI 11.84 - 45.3], leading to the Generalized Estimating Equations (GEE) Model estimate for an odds ratio of 1.4 [95 % CI 0.41 - 5.2], p = 0.06. Cytopath 1 and cytopath 2 are equivalent in terms of specificity (100 % [95 % CI 100 - 100] vs. 100 % [95 % CI 100;100]) and positive predictive value (100 % [95 % CI 100 - 100]. Cytopath 1 is more sensitive than cytopath 2 but not significant (79.66 % [ 95 % CI 69.39 - 89.93] vs. 66.10 % [95 % CI 54.02 - 78.18]). However, the negative predictive value of cytopath 1 is better than that of cytopath 2: 40 % [95 % CI 18.53 - 61.47] vs. 28.57 % [95 % CI 11.84 - 45.3].</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 1","pages":"52-62"},"PeriodicalIF":1.0,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/cc/helm-60-1-helm-2023-0004.PMC10251756.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}