Helminthologia最新文献_第8页

Gnathostoma turgidum - still a mysterious parasite. 肿颌口——仍然是一种神秘的寄生虫。

IF 1.1 4区生物学

Helminthologia Pub Date : 2024-12-12 eCollection Date: 2024-09-01 DOI: 10.2478/helm-2024-0023

Y Nawa, F Delgado-Vargas, S P Díaz-Camacho

引用次数: 0

Infective capacity of the commercial nematode Steinernema carpocapsae parasitizing Aedes aegypti mosquito larvae in Yucatán México. 商业线虫寄生埃及伊蚊幼虫在Yucatán msamicxico的感染能力。

IF 1.1 4区生物学

Helminthologia Pub Date : 2024-12-12 eCollection Date: 2024-09-01 DOI: 10.2478/helm-2024-0028

M B Ávila-López, D Aguirre-Ayala, V M VidaL-Martínez

{"title":"Infective capacity of the commercial nematode Steinernema carpocapsae parasitizing Aedes aegypti mosquito larvae in Yucatán México.","authors":"M B Ávila-López, D Aguirre-Ayala, V M VidaL-Martínez","doi":"10.2478/helm-2024-0028","DOIUrl":"10.2478/helm-2024-0028","url":null,"abstract":"Dengue is currently the most important arbovirosis in the world, affecting over 120 countries in both tropical and sub-tropical areas, with 2500 million people at risk. Due to global warming, it is expected that Aedes aegypti will be able to survive at higher latitudes, increasing the number of people at risk. Therefore, it is crucial to develop control strategies for the mosquito to prevent its environmental impact. The objective of this paper was to determine the concentration of entomopathogenic nematode (EPN) Steinernema carpocapsae that produces the highest percentage mortality of Ae. aegypti larvae under normal environmental conditions in Yucatán (6.9 - 8.7 mg/l; temperature 25.9 - 31.5°C; pH 7.20 - 8.10). The S. carpocapsae mode of action consists of Xenorhabdus nematophila bacterial release in the haemocele insect, which produces septicaemia and consequently kills the mosquito larvae 24 - 48 h after intake. As for the methodology, a commercial EPN strain was used for experimental infection. The experimental design was a 70-h static bioassay that exposed 10 mosquito larvae per experimental unit (400-ml flask and five replicates per treatment) to four nematode concentrations (1250, 2500, 3125 and 3750 nematodes per ml) plus controls. The best treatment was with 1250 nematodes/ml, which caused 54 % mortality with respect to controls after 70 h (one-way ANOVA; F 5.72, 0.05; p < 0.04). We concluded that S. carpocapsae is a promising biological control tool for killing Ae. aegypti larvae at an experimental level. However, it is still necessary to determine its performance at higher volume scales in real-life conditions.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"61 3","pages":"254-262"},"PeriodicalIF":1.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shedding light on neurocysticercosis misinformation in the media. 揭示媒体对神经囊虫病的误传。

IF 1.1 4区生物学

Helminthologia Pub Date : 2024-12-12 eCollection Date: 2024-09-01 DOI: 10.2478/helm-2024-0024

P M Alarcón-Elbal, A Cazzaniga, A E Ahuir-Baraja, M Garijo Toledo

引用次数: 0

Gastrointestinal parasites in captive wild birds in Mineiros, Goiás, Brazil 巴西戈亚斯州米内罗斯圈养野鸟的胃肠道寄生虫

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0019

I. S. Moraes, R. M. P. Moreira, R. B. Duarte, L. S. Prates, B. S. Alves-Ribeiro, H. T. Ferraz, R. C. Pacheco, Í. A. Braga, K. C. Saturnino, D. G. S. Ramos

{"title":"Gastrointestinal parasites in captive wild birds in Mineiros, Goiás, Brazil","authors":"I. S. Moraes, R. M. P. Moreira, R. B. Duarte, L. S. Prates, B. S. Alves-Ribeiro, H. T. Ferraz, R. C. Pacheco, Í. A. Braga, K. C. Saturnino, D. G. S. Ramos","doi":"10.2478/helm-2024-0019","DOIUrl":"https://doi.org/10.2478/helm-2024-0019","url":null,"abstract":"Summary Studying parasites in captive wild birds is vital for their health, well-being, biodiversity preservation, species conservation, and safeguarding of both individual birds and ecosystems. It holds significance for public health by identifying potential zoonotic risks. We aimed to describe the occurrence of gastrointestinal parasites in captive wild birds from a Conservation Institute in Brazilian Cerrado biome. Fresh fecal samples were collected from 17 captive wild birds (Anodorhynchus hyacinthinus, Ara ararauna, Ara chloropterus, Ara macao, Megascops choliba, Pteroglossus castanotis, Ramphastos dicolorus, Ramphastos tucanus and Strix huhula) at a Conservation Institution in Mineiros, state of Goiás. The samples were processed for Willis’ simple flotation and Hoffman’s spontaneous sedimentation examinations to identify parasitic forms of gastrointestinal endoparasites. Macaw aviary birds (Ar. ararauna, Ar. chloropterus and Ar. macao) showed higher positivity, with all six fecal samples positive for helminths or protozoa. In contrast, captive toucans showed only two positive results (P. castanotis and R. dicolorus). An. hyacinthinus showed Ascarididae, Capillarinae and Trematoda eggs; whereas S. huhula had Ascarididae eggs. Regular parasitological examinations are essential for the timely detection and treatment of gastrointestinal infections in captive birds, thereby enhancing overall bird management.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"20 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia 从形态学和遗传学角度鉴定沙特阿拉伯阿拉伯湾中感染双髻鲷鱼（Acanthopagrus bifasciatus）的鳃单基因寄生虫（Diclidophora merlangi）。

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0012

G. Alojayri, S. Al-Quraishy, E. Al-Shaebi, O. B. Mohammed, R. Abdel-Gaber

{"title":"Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia","authors":"G. Alojayri, S. Al-Quraishy, E. Al-Shaebi, O. B. Mohammed, R. Abdel-Gaber","doi":"10.2478/helm-2024-0012","DOIUrl":"https://doi.org/10.2478/helm-2024-0012","url":null,"abstract":"Summary Ectoparasites, particularly monogeneans, negatively affect fish health and growth. This study identified monogenean parasites in the twobar seabream, Acanthopagrus bifasciatus (Sparidae), inhabited the Arabian Gulf (Saudi Arabia). Following that, forty A. bifasciatus fish samples were visually examined for monogeneans. Parasite species were collected from the gills and then analyzed morphometrically, morphologically, and molecularly using the partial regions of the large subunit of ribosomal RNA (28S rRNA) and mitochondrial cytochrome C oxidase subunit I (COI) genes. Fish species were also identified using a DNA barcoding approach based on the COI gene. The monogenean species of Diclidophora merlangi (Diclidophoridae) were found in 45% of the fish species studied. The generic features of the Diclidophora genus distinguish this species. This species discriminated itself from congeners by having a muscular bulb with 17 grooved and recurved hooks, 218±10 (184–267) post-ovarian testes, and four pairs of pedunculated clamps of relative sizes. Partial 28S rRNA sequencing from monogeneans revealed that they grouped with members of the genus Diclidophora, forming a monophyletic group that supported the morphological descriptions. Molecular identification revealed that D. merlangi has a unique barcode made up of a COI sequence. The host identity was established as A. bifasciatus based on the COI gene sequences. Furthermore, a molecular phylogenetic study was performed to determine the phylogenetic affinity of parasite species and fish hosts. This study on Diclidophora species is considered the first record of this genus in the examined area.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"124 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic examination of internal parasites in Iraqi camels (Camelus dromedarius) with molecular focus on Trichostrongylus spp. 对伊拉克骆驼体内寄生虫进行显微镜检查，分子研究重点是三尖虫属（Trichostrongylus spp.

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0011

H. H. Albayati, A. M. Al Khafaji, H. Al-Karagoly, A. Kamel

{"title":"Microscopic examination of internal parasites in Iraqi camels (Camelus dromedarius) with molecular focus on Trichostrongylus spp.","authors":"H. H. Albayati, A. M. Al Khafaji, H. Al-Karagoly, A. Kamel","doi":"10.2478/helm-2024-0011","DOIUrl":"https://doi.org/10.2478/helm-2024-0011","url":null,"abstract":"Summary The camel has played a role in human civilization since its inception and holds significant importance in the customs and agricultural practices of various nations. This study examined the prevalence of internal parasitic infestations in camels within the Al-Diwaniyah and Al-Najaf provinces of Iraq from December 2021 to September 2022. A total of 200 fecal samples were randomly collected from farm camels, revealing that these animals were affected by one or more types of intestinal parasites. Nematodes exhibited the highest prevalence at 56 %, followed by Protozoa at 28.5 %, Cestodes at 14.5 %, and Trematodes at 1 %. Among these parasites, Trichostrongylus spp. had the highest percentage at 33 %, followed by Moneizia benedeni (12.5 %), Fasciola hepatica (10.5 %), Strongyloides spp. (8 %), Giardia spp. (7 %), Nematodirus spp. (6 %), and Eimeria spp. (6 %). Furthermore, mixed-species or single-species infections in camels were observed, including Anoplcephala perfoliata (4 %), Haemonchus spp. (3.5 %), Dictyocaulus spp. (3 %), Trichuris trichura (2.5 %), Entamoeba spp. (2 %), and Balantidium coli (1 %). Additionally, nested PCR was employed to identify Trichostrongylus spp., with 45.4 % of camels testing positive for this particular parasite.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"84 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovarian balls (Floating ovaries) of Rhadinorhynchus niloticus Mohamadain, 1989 from the Nile perch Lates niloticus Linnaeus, 1758; an electron microscope study 尼罗河鲈鱼（Lates niloticus Linnaeus，1758 年）中 Rhadinorhynchus niloticus Mohamadain, 1989 年的卵巢球（浮动卵巢）；电子显微镜研究

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0014

A. M. El-Siefy, M. H. Ibraheem, S. G. Abd El-Kareem

{"title":"Ovarian balls (Floating ovaries) of Rhadinorhynchus niloticus Mohamadain, 1989 from the Nile perch Lates niloticus Linnaeus, 1758; an electron microscope study","authors":"A. M. El-Siefy, M. H. Ibraheem, S. G. Abd El-Kareem","doi":"10.2478/helm-2024-0014","DOIUrl":"https://doi.org/10.2478/helm-2024-0014","url":null,"abstract":"Summary In Acanthocephala, the ovarian balls (floating ovaries) are distinctive structures found suspended in the fluid-filled metasoma of females and are responsible for egg production and maturation. Those structures have not been studied in Rhadinorhynchus niloticus. We aimed to investigate their ultra-structure by means of scanning and transmission electron microscopy. A total of 30 Lates niloticus fish individuals were collected by fishermen from the River Nile, Egypt, and the isolated adult female specimens were processed for electron microscopy studies. The ovarian balls are elongated and lobulated structures freely scattered in the metasoma. They exhibited three distinct primary structural zones, a central oogonial syncytium, a peripheral cellular zone and a surrounding somatic supporting syncytium. The oogonia, within the central syncytium, give rise to developing oocytes that transform into mature oocytes. The latter enclose some inclusions such as large yolk granules and smaller egg-shell granules. We also describe the process of fertilization within the ovarian ball. The structure of the ovarian ball and the steps of fertilization in R. niloticus are described, for the first time, in the present study.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"26 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ACV with/without IVM: a new talk on intestinal CDX2 and muscular CD34 and Cyclin D1 during Trichinella spiralis infection ACV 加/不加 IVM：螺旋体旋毛虫感染期间肠道 CDX2 和肌肉 CD34 及细胞周期蛋白 D1 的新发现

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0013

E. A. El Saftawy, B. E. Aboulhoda, F. E. Hassan, M. A. M. Ismail, M. A. Alghamdi, S. M. Hussein, N. M. Amin

{"title":"ACV with/without IVM: a new talk on intestinal CDX2 and muscular CD34 and Cyclin D1 during Trichinella spiralis infection","authors":"E. A. El Saftawy, B. E. Aboulhoda, F. E. Hassan, M. A. M. Ismail, M. A. Alghamdi, S. M. Hussein, N. M. Amin","doi":"10.2478/helm-2024-0013","DOIUrl":"https://doi.org/10.2478/helm-2024-0013","url":null,"abstract":"Summary The current study assessed the efficacy of Acyclovir (ACV) and Ivermectin (IVM) as monotherapies and combined treatments for intestinal and muscular stages of Trichinella spiralis infection. One-hundred Swiss albino mice received orally 250 ± 50 infectious larvae and were divided into infected-untreated (Group-1), IVM-treated (Group-2), ACV-treated (Group-3), combined IVM+ACV (Group-4), and healthy controls (Group-5). Each group was subdivided into subgroup-A-enteric phase (10 mice, sacrificed day-7 p.i.) and subgroup-B-muscular phase (10 mice, sacrificed day-35 p.i.). Survival rate and body weight were recorded. Parasite burden and intestinal histopathology were assessed. In addition, immunohistochemical expression of epithelial CDX2 in the intestinal phase and CyclinD1 as well as CD34 in the muscular phase were evaluated. Compared, IVM and ACV monotherapies showed insignificant differences in the amelioration of enteric histopathology, except for lymphocytic counts. In the muscle phase, monotherapies showed variable disruptions in the encapsulated larvae. Compared with monotherapies, the combined treatment performed relatively better improvement of intestinal inflammation and reduction in the enteric and muscular parasite burden. CDX2 and CyclinD1 positively correlated with intestinal inflammation and parasite burden, while CD34 showed a negative correlation. CDX2 positively correlated with CyclinD1. CD34 negatively correlated with CDX2 and CyclinD1. IVM +ACV significantly ameliorated CDX2, CyclinD1, and CD34 expressions compared with monotherapies. Conclusion. T. spiralis infection-associated inflammation induced CDX2 and CyclinD1 expressions, whereas CD34 was reduced. The molecular tumorigenic effect of the nematode remains questionable. Nevertheless, IVM +ACV appeared to be a promising anthelminthic anti-inflammatory combination that, in parallel, rectified CDX2, CyclinD1, and CD34 expressions.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"69 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gastrointestinal parasitic helminths of bats from a cave in Luzon Island, Philippines 菲律宾吕宋岛洞穴中蝙蝠的胃肠道寄生蠕虫

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0016

R. A. J. Duco, G. G. Villancio, V. G. V. Paller, P. A. Alviola

{"title":"Gastrointestinal parasitic helminths of bats from a cave in Luzon Island, Philippines","authors":"R. A. J. Duco, G. G. Villancio, V. G. V. Paller, P. A. Alviola","doi":"10.2478/helm-2024-0016","DOIUrl":"https://doi.org/10.2478/helm-2024-0016","url":null,"abstract":"Summary Bats are often associated with several zoonotic diseases, including helminth infections. Despite their potential detrimental effects on both human and bat populations, information on helminth parasites of Philippine bats remains scarce. Here, we identify gastrointestinal helminths parasitizing bats collected from a cave complex in Cavinti, Laguna Province, Philippines, determine their prevalence (P) and mean intensity (MI), and investigate relationship of helminth prevalence and intensity with bat host characteristics including sex, diet, age, and roosting habit. We isolated 581 individuals of helminths representing six taxa (three trematodes, two nematodes, one cestode) from the gastrointestinal tract of sampled bats. Helminths were observed in 82 out of 172 bats captured (P = 47.67%), with Miniopteruspaululus having the highest infection rate (P = 91.3%) and highest MI (18 ± 4.5/infected bat). Helminths parasitizing endemic bat species from the Philippines, such as Rhinolophus rufus, Rhinolophus inops, Hipposideros pygmaeus, and Ptenochirus jagori were reported here for the first time. We provide new host records for other helminth species and report the presence of two parasites, Plagiorchis sp. and Toxocara sp., known for their zoonotic potential. These data will contribute to efforts in identifying parasites and diseases harbored by bats in the country and develop additional conservation measures for bats.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"160 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, ultrastructural, and phylogenetic analysis of Ascaridia columbae infecting domestic pigeons (Columba livia domestica) 家鸽（Columba livia domestica）蛔虫的形态学、超微结构和系统发育分析

IF 1 4区生物学

Helminthologia Pub Date : 2024-07-21 DOI: 10.2478/helm-2024-0018

M. A. Aldamigh, A. A. Alahmadi, I. M. Al-Turaiki, A. H. Hassan

{"title":"Morphological, ultrastructural, and phylogenetic analysis of Ascaridia columbae infecting domestic pigeons (Columba livia domestica)","authors":"M. A. Aldamigh, A. A. Alahmadi, I. M. Al-Turaiki, A. H. Hassan","doi":"10.2478/helm-2024-0018","DOIUrl":"https://doi.org/10.2478/helm-2024-0018","url":null,"abstract":"Summary Ascaridia species are the most common nematodes infecting pigeons. The current study investigated specific identity of nematode parasites collected from domestic pigeons (Columba livia domestica) in Al-Qassim Region, Saudi Arabia. Out of 354 pigeons, 13.3 % were infected with nematode parasites. The morphological structure and genetic relationship of nematode worms were studied using conventional methods (Light and scanning electron microscopes) coupled with the newly introduced molecular method. Microscopical and ultrastructure observations showed that the present nematode worms belong to the genus Ascaridia and have all the characteristic features of Ascaridia columbae. Moreover, Random Amplifier morphometric (RAPD) PCR analysis revealed that the present A. columbae had a close identity of up to 98.3 % to Ascaridia columbae JX624729 for Cox-1 gene regions, and up to 98.3 % to Ascaridia nymphii LC057210, and Ascaridia galli EF180058 for ITS1-5.8s- ITS2 rDNA gene regions. Phylogenetic analysis supported the placement of this Ascaridia species within Ascaridiidae family with close relationships to other nematode species obtained from GenBank. Finally, our study recommends using molecular analysis in helminths identification as the main methodology for correct identification especially in closely related species.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"253 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0