Helminthologia最新文献

Evaluation of the anthelmintic effectiveness of Cinnamomum verum bark extract in mice naturally infected with Aspiculuris tetraptera: in vitro and in vivo. 肉桂树皮提取物对自然感染四翅曲霉的小鼠的驱虫效果的体外和体内评价。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0012

M Murshed, H Alzaylaee, M M Mares, H M A Aljawdah, S Al-Quraishy

{"title":"Evaluation of the anthelmintic effectiveness of Cinnamomum verum bark extract in mice naturally infected with Aspiculuris tetraptera: in vitro and in vivo.","authors":"M Murshed, H Alzaylaee, M M Mares, H M A Aljawdah, S Al-Quraishy","doi":"10.2478/helm-2025-0012","DOIUrl":"10.2478/helm-2025-0012","url":null,"abstract":"The health of the mice used in research experiments is critical to their performance in obtaining correct and accurate data. The current research was done to determine the anthelmintic action of Cinnamomum verum bark extracts (CVBE) in murine infection with Aspiculuris tetraptera. In vitro: The worms were divided into 6 groups: the 1st control, 2nd with 10 mg/mL of Albendazole as a reference drug, and the 3rd, 4th, 5th, and 6th groups were treated with 25, 50, 100, and 200 mg/mL of CVBE, and the test was done at 15, 30, 60, and 120, minutes In vivo: Utilized was twenty-fi ve adult female (C57BL/6) murine, natural infections with A. tetraptera, were sectioned into fi ve treated, each containing 5 murine: 1St, was the non-treated (negative control), and 2ed was regaled 100 μg/mL. 3rd was regaled 200 μg/kg of CVBE for 5 days. 5th was infection and regaled 10 mg/kg mebendazole for 3 days. The GC-MS analysis of phytochemicals in CVBE alcoholic extract disclosed the availability of 20 effi cacious bioactive compounds accountable for worm death. Mortality was measured a dose- and time-dependent effects starting in 20, 40, 80, 120, and 180 mins. The death rate reached (96 % and 100 %) through 120 and 180 min at engagement 200 and 400 mg/mL of CVE. While the non-treated groups lasted many times without death. This study demonstrated that CVBE was effective and had potent anthelminthic activity.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"102-110"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pilot study on the potency of injectable vs. oral moxidectin formulation to suppress strongyle egg excretion in horses at twice lower dose. 注射莫西菌素与口服莫西菌素抑制马圆形卵排泄的两倍剂量的初步研究。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0018

M Šarkūnas, A Schwahn, K Suleimanova

{"title":"A pilot study on the potency of injectable vs. oral moxidectin formulation to suppress strongyle egg excretion in horses at twice lower dose.","authors":"M Šarkūnas, A Schwahn, K Suleimanova","doi":"10.2478/helm-2025-0018","DOIUrl":"10.2478/helm-2025-0018","url":null,"abstract":"The aim of present study was to examine the potency of moxidectin solution (Cydectin 1 %; Zoetis) and commercially available oral moxidectin gel (Equest®; Zoetis) to suppress the excretion of strongyle eggs in horses over a 6-week period. The horses naturally infected with strongyle nematodes (>500 eggs/g of feces) were divided into two comparable groups according age, sex and weight. On day 0 of the study, horses in Group OT (oral treatment; N=5) were dewormed with moxidectin gel (Equest®; Zoetis; 0.4 mg/kg of b.w.) according to the manufacturer's instructions, and horses in Group IT (intramuscular treatment; N=4) were dewormed with moxidectin injectable solution (Cydectin 1 %; Zoetis; 0.2 mg/kg of b.w.). Stool samples were collected rectally, on day 0, 17, 28, 35, and 42 of the study and examined using a modifi ed McMaster technique (Roepstorff et al., 1998) with modifi cations. The mean strongyle egg excretion has signifi cantly decreased in Group IT (P<0.01) and Group OT (P<0.001) on day 17 post treatment as compared to those on day 0. The effi cacy of oral gel (99.43 %) and injectable moxidectin (99.32 %) formulations was therefore high and comparable and no resistance of strongylids to moxidectin was recorded. On day 35 (P<0.001) and 42 (P<0.05) post treatment, the mean effi cacy was signifi cantly higher in Group IT as compared to Group OT. The present study contributes to the existing knowledge and providing more information on the use of injectable MOX solution for the treatment of strongylid infection in horses.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"87-94"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of pseudocysts in Fascioloides magna infected livers of red deer and fallow deer. 马鹿和黇鹿大片形虫感染肝脏假性囊肿的分布。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0015

D Konjević, P Verzak, K Jerabek, M Bujanić, Z Janicki

{"title":"Distribution of pseudocysts in Fascioloides magna infected livers of red deer and fallow deer.","authors":"D Konjević, P Verzak, K Jerabek, M Bujanić, Z Janicki","doi":"10.2478/helm-2025-0015","DOIUrl":"10.2478/helm-2025-0015","url":null,"abstract":"In Europe, red deer and fallow deer are the defi nitive hosts for the non-native fl uke Fascioloides magna. Upon entering the host, a juvenile fl uke emerges from the metacercariae and travels through the abdominal cavity in search of the liver. The aim of this study was to determine the distribution of F. magna pseudocysts in the livers of red deer and fallow deer, and to investigate whether this distribution varies within the livers according to host species and the severity of infection. In this study, 143 red deer livers and 178 fallow deer livers were collected. Livers were divided into three sections (observed from the diaphragm side): left containing the lobus hepatis sinister, middle containing the lobus caudatus and lobus quadratus, and right one containing the lobus hepatis dexter. Sections were sliced to a 2 cm thickness and analysed. A signifi cantly higher number of pseudocysts was found in the middle section of the liver compared to the left or right sections, while no differences were found between the left and right sections. The odds ratio indicates a 7 times (red deer) and 3.7 times (fallow deer) higher probability of pseudocysts being present in the middle section compared to the left section, or 10 and 3.8 times, respectively, compared to the right section. Red deer livers generally harboured more pseudocysts than fallow deer livers in all sections. Both species exhibited a similar pattern of pseudocyst distribution in the liver, with the middle section appearing to be the most suitable site for pseudocyst localization due to the abundance of blood vessels and bile duct terminations. No relationship was found between the severity of infection and pseudocyst distribution.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"161-165"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution to the study of anthelmintic resistance of sheep gastro-intestinal strongyles on a sheep farm in the Guelma region, north-east Algeria. 对阿尔及利亚东北部Guelma地区一个绵羊农场的绵羊胃肠道圆管抗虫性研究的贡献。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0014

S Djemai, N Zeghilet, L Boultif, O Ayadi, Z R Djafar

{"title":"Contribution to the study of anthelmintic resistance of sheep gastro-intestinal strongyles on a sheep farm in the Guelma region, north-east Algeria.","authors":"S Djemai, N Zeghilet, L Boultif, O Ayadi, Z R Djafar","doi":"10.2478/helm-2025-0014","DOIUrl":"10.2478/helm-2025-0014","url":null,"abstract":"The prolonged use of anthelmintics is sometimes accompanied by a gradual decline in their effectiveness, refl ecting the development of resistant parasite strains. This study was conducted in the region of Guelma (northeastern Algeria) at the end of July and throughout August 2018 to investigate the sensitivity (or resistance) of sheep gastrointestinal nematodes to three anthelmintic products used in the Algerian fi eld. The sheepfold comprises 45 ewes of the Ouled-Djellal breed. The data showed that after treatment with anthelmintics, a signifi cant decrease in gastrointestinal nematode eggs per gram of feces (EPG) was observed in all groups. Resistance and sensitivity were observed in all groups studied. In group 1 (AL-Bendazol 2.5 %: albendazole), sensitivity Faecal Egg Count Reduction (FECR > 95 %) was noted in 2 out of 15 ewes (13 %), while resistance (FECR<95 %) was observed in 13 subjects (86.67 %). In the second group (Valbazen: albendazole), the numbers of cases in sensitivity and resistance were around 5 (33 %) and 10 (67 %), respectively. Group 3 (OXFENIL® 2.265 %) revealed 2 (13 %) cases of sensitivity to oxfendazole and 13 resistance cases (86.67 %). No signifi cant difference was noted in the obtained results (sensitivity and resistance) between the three groups.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"156-160"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of internal parasites in captive ungulates from Lahore parks, Pakistan. 巴基斯坦拉合尔公园圈养有蹄类动物体内寄生虫调查。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0016

S Irum, M Faiz, K Aftab, K Aftab, Mehjabeen, H Zulqarnain, S Gondal

{"title":"Survey of internal parasites in captive ungulates from Lahore parks, Pakistan.","authors":"S Irum, M Faiz, K Aftab, K Aftab, Mehjabeen, H Zulqarnain, S Gondal","doi":"10.2478/helm-2025-0016","DOIUrl":"10.2478/helm-2025-0016","url":null,"abstract":"Wild animals are mostly kept for recreational purposes in zoos and parks. Health-related issues faced by these enclosure animals. The GIT (gastrointestinal tract) of an animal is affected by parasitic infections. The current study was conducted to fi nd out the prevalence and diversity of GI parasites of wild animals enclosed in the different parks and zoos of Lahore. Fresh faecal samples (n=110) of captive ungulate species were collected from Lahore Zoo (n=55), Jallo Park (n=35) and the woodland wildlife park (n=20). Samples obtained from captive ungulates include 23 from black buck, 24 from moufl on sheep, 30 from hog deer, 13 from nilgai and 20 from urial. Methods such as the fl otation concentration technique, the sedimentation technique and the faecal egg count per gram were used to analyze the diversity of endoparasites in faecal samples. Parasitological analyses detect 10 parasites in the faecal sample. Kruskal-Wallis Post Hoc statistical test was used to compare the prevalence of the specifi ed parasitic species among different captive ungulates. The results show that individuals of lower age are more susceptible to infections. The overall diversity of GI parasitic infections in captive ungulates was 77.25 %. Survivability of captive ungulates could be affected by the prevalence of parasitic species. These fi ndings can be used to develop effective health protocols for captive ungulates, thereby reducing the severe consequences of GI parasitic infections in captive wild animals.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"111-119"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First analysis of digenean populations in Seriola dumerili (Risso, 1810), (Teleostei: Carangidae) from the Algerian Coast: New host record for Lecithocladium excisum () Lühe, 1901. 阿尔及利亚海岸Seriola dumerili (Risso, 1810), (Teleostei: Carangidae) digenean种群的首次分析：Lecithocladium excisum（）的新寄主记录[j] . he, 1901。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0017

S Amarache, A Boukadoum, F Tazerouti

{"title":"First analysis of digenean populations in Seriola dumerili (Risso, 1810), (Teleostei: Carangidae) from the Algerian Coast: New host record for Lecithocladium excisum () Lühe, 1901.","authors":"S Amarache, A Boukadoum, F Tazerouti","doi":"10.2478/helm-2025-0017","DOIUrl":"10.2478/helm-2025-0017","url":null,"abstract":"The primary objective of this study is to describe and analyze the digenean fauna of the greater amberjack, Seriola dumerili, in Algeria. To achieve this, a total of 121 specimens of S. dumerili were collected from fi sheries and fi shing ports in different regions off the Algerian coastline. One hundred-eight specimens of S. dumerili were found to be infested, and 7,959 specimens of digenean were collected. Five species belonging to three families were identifi ed. The species involved are Lecithocladium excisum, Parahemiurus merus, Tormopsolus orientalis, Stephanostomum euzeti and Bucephalus gorgon. Tormopsolus orientalis and B. gorgon were recorded for the fi rst time off the Algerian waters. S. euzeti was described for the second time, based on adult worms, since its original description. As for L. excisum, S. dumerili represents a new host record. The data analysis indicated that B. gorgon had the highest values of prevalence (85.95 %), mean abundance (64) and mean intensity (74.46), qualifying it as a core species. Spearman's correlation test revealed a strong positive correlation between the mean abundance and the mean intensity of S. euzeti, as well as the size of the host. These results provide new insights into the digenean fauna of the greater amberjack, thereby enhancing our understanding of parasitic helminths in teleost fi shes along the Algerian coastline.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"144-155"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal parasites in rodents from five different Himas in Lebanon. 黎巴嫩五种不同Himas啮齿动物的肠道寄生虫。

IF 1.2 4区生物学

Helminthologia Pub Date : 2025-09-30 eCollection Date: 2025-06-01 DOI: 10.2478/helm-2025-0011

M Abi Said, T Maroun, H Shaib

{"title":"Intestinal parasites in rodents from five different Himas in Lebanon.","authors":"M Abi Said, T Maroun, H Shaib","doi":"10.2478/helm-2025-0011","DOIUrl":"10.2478/helm-2025-0011","url":null,"abstract":"Rodent and their parasites serve as a reliable indicator of ecosystem health, which is critical in determining the structure of ecological communities. Therefore, gaining a thorough comprehension of the diversity of intestinal parasites and the factors infl uencing their interaction is of primary importance. This study assesses the diversity of gastrointestinal parasites in wild rodents in Lebanon. Two thousand fi ve hundred trapping nights were carried out in fi ve Himas in Lebanon during the spring and fall of 2022. A total of 205 rodents, including Apodemus mystacinus, Apodemus flavicollis, Apodemus hermonensis, Microtus guentheri, Rattus rattus, and Mus musculus, were trapped and examined for intestinal parasites. Nine intestinal parasites were isolated, including one cestode species, Hymenolepis diminuta (11.7 %), and eight nematodes: Ascaris lumbricoides (31.7 %), Trichuris muris (7.8 %), Aspiculuris tetraptera (7.3 %), Heligmosomoides polygyrus (6.8 %), Syphacia muris (3.9 %), Syphacia obvelata (3.4 %), Capillaria spp. (2.4 %), and Physaloptera spp (0.5 %). The species and gender did not infl uence the number of rodents infected with these parasites. However, the season impacted the number of rodents infected with A. lumbricoides, T. muris, and S. muris. Infection of rodents with H. diminuta and A. lumbricoides was infl uenced by the site of trapping. Among the collected parasites, A. lumbricoides, H. diminuta, and Syphacia obvelata are considered zoonotic. This study showed that preserving parasite-host dynamics and biological cycles depends heavily on environmental stability. This project will further advance the understanding of rodent parasites and support future studies on endoparasites in Lebanon and the region.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 2","pages":"120-134"},"PeriodicalIF":1.2,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors affecting the prevalence of endoparasites in pre-weaning calves in a warm humid climate of Mexico. 影响墨西哥温暖潮湿气候下断奶前小牛体内寄生虫流行的因素。

IF 1.1 4区生物学

Helminthologia Pub Date : 2025-05-24 eCollection Date: 2025-03-01 DOI: 10.2478/helm-2025-0003

C Y León-González, R González-Garduño, J M Hernández-Domínguez, F L Peña-Escalona, A Villa-Mancera, L Aguilar-Marcelino

{"title":"Factors affecting the prevalence of endoparasites in pre-weaning calves in a warm humid climate of Mexico.","authors":"C Y León-González, R González-Garduño, J M Hernández-Domínguez, F L Peña-Escalona, A Villa-Mancera, L Aguilar-Marcelino","doi":"10.2478/helm-2025-0003","DOIUrl":"10.2478/helm-2025-0003","url":null,"abstract":"Parasitic diseases are a major challenge for livestock in warm climates, causing significant economic losses, particularly in young animals. Gastrointestinal nematodes (GIN) are highly prevalent, prompting us to investigate factors affecting the prevalence of endoparasites in pre-weaning calves in a warm, humid climate in Mexico. Fecal samples were analyzed to assess fecal egg count (FEC) dynamics and endoparasite prevalence. The prevalence of trematodes (Fasciola hepatica and paramphistomids) was determined on 12 beef cattle farms in the rainy season from May to September 2022 and July to November 2023. Nematode prevalence was monitored on four farms during 2022 and 2023. In addition, on one farm, the FEC was compared between dewormed and untreated groups. Coprocultures were conducted to obtain third-stage larvae for species identification based on morphology. The prevalence of F. hepatica was 12 % (77/640), while paramphistomids had a prevalence of 20.5 % (131/640). Trichostrongylids showed a prevalence exceeding 30 %, with maximum values reaching 93 %. The predominant species identified from coprocultures were Cooperia spp., Oesophagostomum spp., and Haemonchus spp. Dewormed calves exhibited similar nematode eggs per gram of feces (EPG) to the untreated group, and nematode EPG in calves tended to decrease with age. In conclusion, age, sex, and farm management practices influence the prevalence of nematodes and trematodes. Nematode prevalence exceeded 30 % for most of the year, while trematode prevalence ranged from 12 % to 20 %.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 1","pages":"30-39"},"PeriodicalIF":1.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evaluation of hydro-ethanolic seed extract of Zanthoxyllum armatum DC to establish its mechanism of action for paralysis of Paramphistomum cervi in continuation with in vivo experimentation in naturally infected goat. 在自然感染山羊体内实验的基础上，体外评价花椒籽水乙醇提取物对宫颈旁口麻痹的作用机制。

IF 1.1 4区生物学

Helminthologia Pub Date : 2025-05-24 eCollection Date: 2025-03-01 DOI: 10.2478/helm-2025-0008

S Hazarika, C C Barua, P Mohan, A Das, M Hazorika, S Tamuly, P Pathak, B Borah, D Phangchupi, A Chakraborty, S George

{"title":"In vitro evaluation of hydro-ethanolic seed extract of Zanthoxyllum armatum DC to establish its mechanism of action for paralysis of Paramphistomum cervi in continuation with in vivo experimentation in naturally infected goat.","authors":"S Hazarika, C C Barua, P Mohan, A Das, M Hazorika, S Tamuly, P Pathak, B Borah, D Phangchupi, A Chakraborty, S George","doi":"10.2478/helm-2025-0008","DOIUrl":"10.2478/helm-2025-0008","url":null,"abstract":"The study aims to evaluate the anthelmintic potential of hydro-ethanolic seed extract of Zanthoxyllum armatum (HESEZA) DC against Paramphistomum cervi, a rumen amphistome highly prevalent in North-East India. In vitro and in vivo experiments were carried out to evaluate the anthelmintic property of HESEZA and the mechanism of action against Paramphistomum cervi. In vitro motility and different biochemical estimations like glucose uptake, glycogen content, lactic acid production and acetylcholinesterase (AChE) activity of the extract and standard drug treated worms were studied to explore the mechanism of action. HESEZA @ 3000 μg/ml showed complete death of worms within 1 hr of exposure. Glucose uptake was reduced significantly (P<0.05) by the HESEZA @ 300, 1000 and 3000 μg/ml. Similarly, glycogen content was found to decrease significantly (P<0.05) by the HESEZA @ 300, 1000 and 3000 μg/ml. However, lactic acid production was increased significantly (P<0.05) by the HESEZA @ 300, 1000 and 3000 μg/ml. On the contrary, AChE activity of the P. cervi was reduced significantly (P<0.05) @ 3000 μg/ml by the HESEZA. An in vivo experiment of HESEZA @ 150 and 250 mg/kg body weight in naturally infected goats were accomplished with complete reduction of EPG count on 28th day of post-treatment of the extract @ 250 mg/kg b. wt. All the haematological and biochemical parameters were estimated during in vivo experimentation in goats with HESEZA.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 1","pages":"19-29"},"PeriodicalIF":1.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redescription of Laimydorus siddiqii Baqri & Jana, 1982 (Dorylaimida: Dorylaimidae) with its first molecular study. 《睡蝉科》（Laimydorus siddiqii Baqri & Jana, 1982）的重新描述及其首次分子研究。

IF 1.1 4区生物学

Helminthologia Pub Date : 2025-05-24 eCollection Date: 2025-03-01 DOI: 10.2478/helm-2025-0006

A Ansari, A H Bhat, Z Imran, A K Chaubey, D Fouad, G Pundir

{"title":"Redescription of Laimydorus siddiqii Baqri & Jana, 1982 (Dorylaimida: Dorylaimidae) with its first molecular study.","authors":"A Ansari, A H Bhat, Z Imran, A K Chaubey, D Fouad, G Pundir","doi":"10.2478/helm-2025-0006","DOIUrl":"10.2478/helm-2025-0006","url":null,"abstract":"A nematode population (SF7) belonging to the genus Laimydorus siddiqi, 1969, was isolated from the soils of sugarcane fields in the Haridwar district of Uttarakhand, India. Based on morphological, morphometrical, and molecular studies, the nematode was identified as Laimydorus siddiqii. This work represents the first molecular report of this species. Laimydorus siddiqui has a 2.6 to 3.08 mm long body, with a truncate lip region, linear odontostyle, double guiding ring, female genital system amphidelphic, longitudinal vulva, and females with a very long, filiform tail. Males have 56 - 63 μm long spicules and 28 - 29 ventromedial supplements. Molecular analyses utilizing segments of the 28S rDNA placed Laimydorus siddiqi in a clade with Laimydorus zehsaziani from Iran and other representatives of the genera Labronema, Dorylaimus, Prodorylaimus, Calcaridorylaimus, Mesodorylaimus, and Aporcelamellus. However, there is no available 18S rRNA sequence of any known species of Laimydorus, and our sequence is the first SSU rRNA of this genus. These molecular findings support the taxonomic positioning of Laimydorus siddiqui within the broader phylogenetic framework of the family Dorylaimidae, highlighting its relationships with closely related genera.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"62 1","pages":"62-71"},"PeriodicalIF":1.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12117614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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