Evaluation of the anthelmintic effectiveness of Cinnamomum verum bark extract in mice naturally infected with Aspiculuris tetraptera: in vitro and in vivo.
M Murshed, H Alzaylaee, M M Mares, H M A Aljawdah, S Al-Quraishy
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引用次数: 0
Abstract
The health of the mice used in research experiments is critical to their performance in obtaining correct and accurate data. The current research was done to determine the anthelmintic action of Cinnamomum verum bark extracts (CVBE) in murine infection with Aspiculuris tetraptera. In vitro: The worms were divided into 6 groups: the 1st control, 2nd with 10 mg/mL of Albendazole as a reference drug, and the 3rd, 4th, 5th, and 6th groups were treated with 25, 50, 100, and 200 mg/mL of CVBE, and the test was done at 15, 30, 60, and 120, minutes In vivo: Utilized was twenty-fi ve adult female (C57BL/6) murine, natural infections with A. tetraptera, were sectioned into fi ve treated, each containing 5 murine: 1St, was the non-treated (negative control), and 2ed was regaled 100 μg/mL. 3rd was regaled 200 μg/kg of CVBE for 5 days. 5th was infection and regaled 10 mg/kg mebendazole for 3 days. The GC-MS analysis of phytochemicals in CVBE alcoholic extract disclosed the availability of 20 effi cacious bioactive compounds accountable for worm death. Mortality was measured a dose- and time-dependent effects starting in 20, 40, 80, 120, and 180 mins. The death rate reached (96 % and 100 %) through 120 and 180 min at engagement 200 and 400 mg/mL of CVE. While the non-treated groups lasted many times without death. This study demonstrated that CVBE was effective and had potent anthelminthic activity.
期刊介绍:
Helminthologia (HELMIN), published continuously since 1959, is the only journal in Europe that encompasses the individual and collaborative efforts of scientists working on a different topics of human, veterinary and plant helminthology. The journal responsibility is to enrich the theoretical and practical knowledge in very specific areas and thus contribute to the advancements in human and veterinary medicine and agronomy. Taking the advantage of comprehensive and multidisciplinary approaches journal still maintains its original spirit and is principal source of fresh scientific information regarding helminths, endoparasites and plant parasites. Addressing the most up-to date topics journal gained rightful and exceptional place next to the other high-quality scientific journals publishing in its field.