HelminthologiaPub Date : 2022-12-01DOI: 10.2478/helm-2022-0040
M Sočan, E Štromajer, M Ravnik, M Mrzel, E Grilc, I Grmek Košnik
{"title":"<i>Enterobius Vermicularis</i> Infection: A Cross-sectional Study in Preschool and School Children in the North-Western Part of Slovenia.","authors":"M Sočan, E Štromajer, M Ravnik, M Mrzel, E Grilc, I Grmek Košnik","doi":"10.2478/helm-2022-0040","DOIUrl":"https://doi.org/10.2478/helm-2022-0040","url":null,"abstract":"<p><p><i>Enterobius vermicularis</i> is a prevalent intestinal nematode. The objective of the research was to study enterobiasis prevalence in symptomatic children <15 years of age attending community health center in North - Western part of Slovenia in years 2017 - 2022. Perianal tape tests were performed on three consecutive days. The overall prevalence was 34.2 % (296 out of 864 children included). The mean age of children positive for <i>E. vermicularis</i> was 5.77 (95 % CI: 5.51 - 6.04) and 4.74 (95 % CI: 4.54 - 4.95), p<0.001 for children with negative test results. The positivity rate was not significantly different for boys compared to girls (boys 37.0 %, 95 % CI: 32.4 % - 41.8 %, girls 31.8 %, 95 % CI: 27.6 % - 36.2 %, p=0.107). The number of boys with all three samples positive in a sample set was higher compared to girls (p - value 0.002). Family size affected the positivity rate - the mean number of siblings was higher in positive children. Significant association with <i>E. vermicularis</i> infection was proven by the presence of anal pruritus and absence of abdominal discomfort. High <i>E. vermicularis</i> warrants careful monitoring of trends and public health response. It is necessary to encourage the use of hygiene measures in schools and empower parents to recognize enterobiasis timely.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 4","pages":"357-363"},"PeriodicalIF":1.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/fd/helm-59-357.PMC9979069.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10855189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0018
Yu A Belov, T V Tabakaeva, D V Pankratov, E M Shchelkanov, A L Surovyi, I A Popov, A V Tabakaev, L V Zheleznova, I V Galkina, M Yu Shchelkanov
{"title":"Endoparasites of Wild Boars (<i>Sus Scrofa</i>) in Primorsky Krai, Russia.","authors":"Yu A Belov, T V Tabakaeva, D V Pankratov, E M Shchelkanov, A L Surovyi, I A Popov, A V Tabakaev, L V Zheleznova, I V Galkina, M Yu Shchelkanov","doi":"10.2478/helm-2022-0018","DOIUrl":"https://doi.org/10.2478/helm-2022-0018","url":null,"abstract":"<p><p>This study identified helminthic species among wild boars (<i>Sus scrofa</i>) in Primorsky Krai, Russia. In total, 66 fecal samples were taken from wild boars and examined using the floatation-sedimentation method to identify helminths eggs and protozoan cysts. Age and sex were estimated for each host animal investigated. The helminthic fauna of the wild boars examined involved six parasite genera, but 4 are helminths and 2 are protozoans: the nematodes <i>Metastrongylus spp</i>., <i>Trichuris suis</i>, <i>Capillaria sp</i>. and <i>Ascaris suum</i>; and the protozoan parasites <i>Eimeria</i> sp. and <i>Cystoisospora suis</i>. The most prevalent parasite was <i>Metastrongylus</i> spp. (13.6 %) followed by <i>Trichuris suis</i> (7.6 %). The other parasites found were <i>Eimeria</i> sp. (3 %), <i>Ascaris suum</i> (3 %), <i>Capillaria</i> sp. (1.5 %) and <i>Cystoisospora suis</i> (1.5 %). Not found positive correlation between the host's age and sex and the parasite prevalence. This was the first detailed study on helminths infections among wild boars in Primorsky Krai.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"165-169"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4b/7a/helm-59-165.PMC9444208.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40364466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0013
S L Honcharov, N M Soroka, M V Galat, O V Zhurenko, A I Dubovyi, V I Dzhmil
{"title":"<i>Eustrongylides</i> (Nematoda:Dioctophymatidae): Epizootology and Special Characteristics of The Development Biology.","authors":"S L Honcharov, N M Soroka, M V Galat, O V Zhurenko, A I Dubovyi, V I Dzhmil","doi":"10.2478/helm-2022-0013","DOIUrl":"https://doi.org/10.2478/helm-2022-0013","url":null,"abstract":"<p><p><i>Eustrongylides</i> spp. are the nematodes from the Dioctophymatidae family which are potentially dangerous for the mammal's heath. The first aim of the present research is to describe the biology, developmental characteristics and epizootology of the <i>Eustrongylides</i> spp. nematode. Eustrongylidosis is a parasitic disease commonly occurring in the countries with temperate continental, tropical and subtropical climate. The <i>Eustrongylides</i> spp. parasites are biohelmints with a complex life cycle. The development of the nematodes takes place in the aquatic environment and includes a vast range of intermediate, accidental and definitive hosts. It has also been reported in humans. The second aim of the research is to describe the clinical and pathomorphological changes in organs and tissues of the infected animals, and influence of biotic and abiotic factors on formation of the natural centers of spreading of eustrongylidosis; diagnostics of eustrongylidosis.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"127-142"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/31/82/helm-59-127.PMC9444202.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40365937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0019
E Shokoohi
{"title":"Observation on <i>Hemicriconemoides Brachyurus</i> () Chitwood & Birchfield, 1957 Associated with Grass in South Africa.","authors":"E Shokoohi","doi":"10.2478/helm-2022-0019","DOIUrl":"https://doi.org/10.2478/helm-2022-0019","url":null,"abstract":"<p><p><i>Hemicriconemoides brachyurus</i> was identified morphologically, using body annuli number (103105), stylet length (49-52 μm), tail conical with small rounded lobe, spermatheca with round sperm, and VL/VB (1.3). Molecular analysis was also undertaken, based on the 18S rDNA region, and the obtained DNA sequence data confirmed the present population from South Africa as <i>Hemicriconemoides</i>. Phylogenetic analysis using the Bayesian inference method places this population close to <i>H. fujianensis</i> from China (MH444620-21; MH444626-28). To the best of our knowledge, this is the first report of the 18S rDNA sequence for <i>H. brachyurus</i>.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"210-216"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/69/helm-59-210.PMC9444206.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40364467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0021
M Chaouadi, F Scarpa, I Azzena, P Cossu, K Harhoura, M Aissi, F Tazerouti, G Garippa, P Merella, M Casu, D Sanna
{"title":"New Insights Into the Genetic Variability of <i>Fasciola Hepatica</i> (Trematoda) in Algeria and Relationships with Other Geographic Regions Revealed By Mitochondrial DNA.","authors":"M Chaouadi, F Scarpa, I Azzena, P Cossu, K Harhoura, M Aissi, F Tazerouti, G Garippa, P Merella, M Casu, D Sanna","doi":"10.2478/helm-2022-0021","DOIUrl":"https://doi.org/10.2478/helm-2022-0021","url":null,"abstract":"<p><p>This study aims to investigate the level of genetic variability of <i>Fasciola hepatica</i> flukes isolated from cattle in Algeria and to determine the phylogenetic and phylogeographic relationships with sequences isolated worldwide. Mitochondrial (Cytochrome c Oxidase subunit I gene - COI) and nuclear markers (Internal Transcribed Spacers of nuclear ribosomal DNA - ITS) for 24 <i>F. hepatica</i> flukes isolated from 12 cattle in North Algeria were characterised. Only two haplotypes were obtained for the COI gene, resulting in a low level of genetic variation. The analysis of variation among the COI sequences isolated from around the world did not show high levels of genetic divergence, and the phylogenetic analysis revealed a genetic similarity among <i>F. hepatica</i> isolates from different areas of the world. The analysis of the ITS region showed a low level of variability, which prevented obtaining informative phylogenetic and phylogeographic results. The present study also revealed that specimens of <i>F. hepatica</i> are genetically similar in different hosts, indicating that the genetic structure among populations of this parasite is not influenced by the host species. The low levels of genetic variation for COI and ITS regions among fluke isolates from all continents are consistent with a common origin for the flukes' worldwide distribution.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"152-164"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8f/7a/helm-59-152.PMC9444205.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40365936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0015
R Kerroucha, I Medjoual, L Bourguig, K Senouci
{"title":"Identification of The Main Intestinal Helminths of Local Breed Chickens (<i>Gallus Gallus Domesticus</i> Linnaeus, 1758) Reared in Traditional Mode in The Oran Region.","authors":"R Kerroucha, I Medjoual, L Bourguig, K Senouci","doi":"10.2478/helm-2022-0015","DOIUrl":"https://doi.org/10.2478/helm-2022-0015","url":null,"abstract":"<p><p>In order to gain a better etiological and epidemiological knowledge of the parasitic diseases of local breed chickens reared in extensive (traditional) mode, a study was carried out in the Oran region during the periods from February 2020 to April 2020. Ten chickens were examined by the helminthologic autopsy method to identify parasitic helminths in the digestive tract. Four species of helminths have been identified: two species of nematodes: <i>Ascaridia galli</i> and <i>Heterakis gallinarum</i> and two species of cestodes: <i>Raillietina cesticillus</i> and <i>Raillietina tetragona</i>. Six chickens were carriers of the parasites, for an overall level of infestation of 60 % with an average infestation of 7 parasites per chicken. The rate of parasitism and infestation varied from species to species, <i>Heterakis gallinarum</i> being the most dominant species. The estimate of the infestation rate by each group shows a predominance of nematodes with 62 parasites (88.5 %) compared to cestodes (8 parasites) with a significantly higher difference (P <0.05). The results obtained show that chickens of the local <i>Gallus gallus domesticus</i> breed in the Oran region are heavily infested by parasites including <i>Heterakis gallinarum</i>, the predominant species.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"170-178"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/e8/helm-59-170.PMC9444204.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40364465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0014
T Brázová, P Kováčik, M Matoušková, M Oros
{"title":"Nematodes As Soil Stress Indicators for Polycyclic Aromatic Hydrocarbons: a Review.","authors":"T Brázová, P Kováčik, M Matoušková, M Oros","doi":"10.2478/helm-2022-0014","DOIUrl":"https://doi.org/10.2478/helm-2022-0014","url":null,"abstract":"<p><p>Polycyclic aromatic hydrocarbons (PAHs) are an important group of organic pollutants present in all parts of the environment, affecting ecosystems and human health. PAHs, which have a strong affinity for organic carbon, are found in large quantities in soil, which is one of the most important sinks for these contaminants. Their impact on the soil biotic compartments depends on a number of different factors in combination with PAH behaviour and can be assessed using soil monitoring. Soil fauna have already shown excellent properties for biomonitoring of contaminants with most promising indicator frameworks based on nematodes, which are involved in essential processes in this environment. Nematodes respond to PAHs at multiple levels, including molecular, individual and community levels. At the molecular level, this is associated with activation of metabolic pathways for xenobiotics and increased demand for energy and resources. At the individual level, this is reflected in the slowing down of various physiological processes, which has consequences at the individual and community level for sensitive taxa. In this review, the toxicity and the direct and indirect effects of PAHs on soil nematode communities are discussed. It also considers the perspectives and challenges in assessing the toxicity of PAHs and their indication using soil nematodes.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"117-126"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/aa/48/helm-59-117.PMC9444203.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40365933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0020
N Miladinović-Tasić, A Schmidt-Rhaesa, V Petrović
{"title":"Human Encounter with a Horsehair Worm (Nematomorpha): Is There a Reason To Worry?","authors":"N Miladinović-Tasić, A Schmidt-Rhaesa, V Petrović","doi":"10.2478/helm-2022-0020","DOIUrl":"https://doi.org/10.2478/helm-2022-0020","url":null,"abstract":"<p><p>We report here a specimen of the horsehair worm (Gordiida, Nematomorpha) <i>Spinochordodes bacescui</i> (Căpuşe, 1965) found in a patient´s urine. This is the first record of this species from Serbia and the sixth gordiid species known in Serbia. It is discussed that there is no evidence that gordiids are parasites of humans. Instead, gordiids parasitize terrestrial insects, which release their mature gordiid parasite into water and may thereby also use containers in human surroundings. Pseudoparasites, such as <i>Spinochordodes bacescui</i>, do not pose a threat to human health nor are they a serious public health issue.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"200-203"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c9/31/helm-59-200.PMC9444207.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40365934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0017
M Zaragoza-Vera, R González-Garduño, L Brito-Argáez, A J Aguilar-Caballero, C V Zaragoza-Vera, G Arjona-Jiménez, V M Loyola-Vargas, V Aguilar-Hernández, O M Torres-Chable
{"title":"Identification of Somatic Proteins in <i>Haemonchus Contortus</i> Infective Larvae (L<sub>3</sub>) and Adults.","authors":"M Zaragoza-Vera, R González-Garduño, L Brito-Argáez, A J Aguilar-Caballero, C V Zaragoza-Vera, G Arjona-Jiménez, V M Loyola-Vargas, V Aguilar-Hernández, O M Torres-Chable","doi":"10.2478/helm-2022-0017","DOIUrl":"https://doi.org/10.2478/helm-2022-0017","url":null,"abstract":"<p><p><i>Haemonchus contortus</i> is considered the most pathogenic nematode in sheep production systems based on grazing. Comparing infective larvae (L<sub>3</sub>) with adult parasites can lead to the identification of proteins that play an important role in parasite-host interactions. In this study, we report a list of <i>H. contortus</i> somatic proteins and made a comparative analysis of somatic proteins of L<sub>3</sub> and adult worms. L<sub>3</sub> and adult parasites were subjected to protein extraction and subsequently to peptide fractionation. Peptides were analysed by mass spectrometry and LC-MS/MS data analysis. Data analysis and search on SEQUEST and MASCOT against <i>H. contortus</i> from the WormBase ParaSite database resulted in the identification of 775 unique peptide sequences corresponding to 227 proteins at 1 % FDR. From these, 18 proteins were specific to L<sub>3</sub> and 63 to adult parasites. The gene ontology (GO) enrichment analysis of the proteins specific to L<sub>3</sub> and adult worms to gain insight into cellular components, molecular functions and biological processes that affect the parasite-host interaction showed some differences between the two parasite stages. The list of proteins found provides a database to identify target proteins that could be useful as biomarkers of the infection or in the generation of anthelmintic drugs that inhibit proteins essential for the establishment of the infection and the survival of adult parasites. They can also serve as new candidates for vaccine research.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"143-151"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/cb/helm-59-143.PMC9444209.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40364468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HelminthologiaPub Date : 2022-09-03eCollection Date: 2022-06-01DOI: 10.2478/helm-2022-0016
J A Panti-May, D I Hernández-Mena, H A Ruiz-Piña, V M Vidal-Martínez
{"title":"Occurrence of <i>Ancylostoma Caninum</i> from a Gray Fox <i>Urocyon Cinereoargenteus</i> in Southeastern Mexico.","authors":"J A Panti-May, D I Hernández-Mena, H A Ruiz-Piña, V M Vidal-Martínez","doi":"10.2478/helm-2022-0016","DOIUrl":"https://doi.org/10.2478/helm-2022-0016","url":null,"abstract":"<p><p>The hookworm <i>Ancylostoma caninum</i> is a common nematode of wild and domestic canids worldwide. In Mexico, there are few records of helminths in wild canids, especially in the southeastern region. The aim of the present study was to examine the helminths from a gray fox <i>Urocyon cinereoargenteus</i> in southeastern Mexico. A road-killed female gray fox found in Merida, Yucatan, Mexico, was examined for helminths. Only nematodes were found in the intestine of the gray fox and identified using morphological studies and molecular analysis of 28S rRNA gene fragments. The characteristics exhibited by the nematode specimens were in accordance with descriptions of <i>A. caninum</i>: e. g. oral opening with a pair of prominent chitinous plates bearing three pairs of ventral teeth, lateral rays with a common trunk, dorsal ray divided into two branches with each branch terminating in three digitations. BLAST analysis of the 28S sequence showed similarity and coverage values of 99.8 % and 100 %, respectively, with a sequence of <i>A. caninum</i> from the domestic dog <i>Canis familiaris</i> in Australia. The genetic distance between the Australian specimen and the Yucatan specimen of <i>A. caninum</i> was 0.1 %, that is, they were only different in a single nucleotide. The gray fox examined in this study was found close to a rural community where <i>A. caninum</i> has been recorded from domestic dogs, which could be the source of infection. Our study increases the distribution of this nematode parasitizing the gray fox in Mexico and provides the first nucleotide sequence of <i>A. caninum</i> from the gray fox.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 2","pages":"204-209"},"PeriodicalIF":1.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/80/e6/helm-59-204.PMC9444201.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40365938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}