Helminthologia最新文献

{"title":"Does <i>in Vitro</i> and <i>in Vivo</i> Exposure To Medicinal Herbs Cause Structural Cuticular Changes in <i>Haemonchus Contortus</i>?","authors":"M Komáromyová,&nbsp;D Barčák,&nbsp;A Königová,&nbsp;M Urda Dolinská,&nbsp;M Várady","doi":"10.2478/helm-2022-0023","DOIUrl":"https://doi.org/10.2478/helm-2022-0023","url":null,"abstract":"<p><p>The health and production of small ruminants in constantly menaced by parasitic infections, especially those caused by the blood-sucking gastrointestinal nematode <i>Haemonchus contortus</i>. The aim of this study was to assess the structural cuticular changes in adult <i>H. contortus</i> induced by the use of extracts from local medicinal plants and to examine their ovicidal activity. Previous studies have confirmed the beneficial effect of herbal mixtures in preventing haemonchosis in lambs by lowering fecal egg production and improving immunocompetence. We exposed adult <i>H. contortus</i> to Herbmix (a mixture of medicinal plants) under <i>in vivo</i> and <i>in vitro</i> conditions for observation by scanning electron microscopy (SEM). For the <i>in vivo</i> observations, adult worms were isolated from the abomasa of experimentally infected lambs from a Herbmix group and a control group. Surface structure did not differ significantly between the exposed and control groups. The ovicidal activity of an aqueous Herbmix extract was assessed <i>in vitro</i>, establishing the inhibition of hatching with an ED₅₀ of 6.52 mg/mL. Adult worms for <i>in vitro</i> examination were isolated from experimentally infected lambs and incubated in Herbmix aqueous extracts for 24 h. SEM observations indicated that none of the worms had prominent ultrastructural changes on their cuticles. This study suggests that previously demonstrated antiparasitic effects of medicinal plants did not negatively affect adult parasites by damaging their external structures.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 3","pages":"265-274"},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/68/helm-59-265.PMC9831516.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10671894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of a New Genus and Species of Allocreadiidae (Trematoda) in Mexico: <i>Mesoamericatrema Magnisacculus</i> n. Gen. n. Sp.","authors":"B Mendoza-Garfias,&nbsp;J G García-Teh,&nbsp;J M Caspeta-Mandujano,&nbsp;V M Vidal-Martínez,&nbsp;D I Hernández-Mena","doi":"10.2478/helm-2022-0029","DOIUrl":"https://doi.org/10.2478/helm-2022-0029","url":null,"abstract":"<p><p>The trematodes of Allocreadiidae are mainly parasites of freshwater fish and the family contains 18 valid genera. Since 2004, 4 genera have been described in the Americas: <i>Paracreptotrema</i>, <i>Paracreptotrematoides</i>, <i>Pseudoparacreptotrema</i> and <i>Auriculostoma</i>, of which the latter was recently synonymized with <i>Creptotrema</i> by Franceschini et al., (2021). During a study in 2018 on fish parasites of Lacantún River, Chiapas, Mexico, allocreadiids were collected from the intestine of <i>Atherinella alvarezi</i> and morphological differences with other genera of the family were immediately noted. These differences were corroborated with molecular data and phylogenetic analyses, which indicated that it was a new genus and species. The objective of this study is to describe the new taxon, which we name <i>Mesoamericatrema magnisacculus</i> n. gen., n. sp. as well as to clarify its phylogenetic relationships, host specificity, and geographical distribution. For this, we carried out a meticulous study of the morphology and compared the new taxon with its congeners. We also obtained sequences of the 28S and ITS from the ribosomal RNA region that were used in generating molecular phylogenies and in calculating genetic distances with sequences of other species of Allocreadiidae available in the Genbank. The new genus is mainly characterized by having a very large cirrus sac in relation to the length of its body and nests in a clade that groups <i>Creptotrema</i>, <i>Creptotrematina</i>, <i>Paracreptotrema</i>, <i>Paracreptotrematoides</i>, <i>Pseudoparacreptotrema</i> and <i>Wallinia</i>, all of which have a Neotropical affinity in terms of their geographic and host distribution. <i>Mesoamericatrema</i> n. gen. is the fifth genus of Allocreadiidae to be described in the Americas since 2004, the third discovered in Mexico since 2016, and the first to be described as parasitizing Atheriniformes, which indicates that Middle America is a region where important speciation events have occurred in freshwater fish parasites.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 3","pages":"284-300"},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/10/helm-59-284.PMC9831523.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9172650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Synthesis of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine Kinase (GNE), α-dystroglycan, and β-galactoside α-2,3-sialyltransferase 6 (ST3Gal6) By Skeletal Muscle Cell As a Response To Infection with <i>Trichinella Spiralis</i>.","authors":"R Milcheva,&nbsp;K Todorova,&nbsp;A Georgieva,&nbsp;S Petkova","doi":"10.2478/helm-2022-0027","DOIUrl":"https://doi.org/10.2478/helm-2022-0027","url":null,"abstract":"<p><p>The Nurse cell of the parasitic nematode <i>Trichinella spiralis</i> is a unique structure established after genetic, morphological and functional modification of a small portion of invaded skeletal muscle fiber. Even if the newly developed cytoplasm of the Nurse cell is no longer contractile, this structure remains well integrated within the surrounding healthy tissue. Our previous reports suggested that this process is accompanied by an increased local biosynthesis of sialylated glycoproteins. In this work we examined the expressions of three proteins, functionally associated with the process of sialylation. The enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) is a key initiator of the sialic acid biosynthetic pathway. The α-dystroglycan was the only identified sialylated glycoprotein in skeletal muscles by now, bearing sialyl-α-2,3-Gal-β-1,4-Gl-cNAc-β-1,2-Man-α-1-O-Ser/Thr glycan. The third protein of interest for this study was the enzyme β-galactoside α-2,3-sialyltransferase 6 (ST3Gal6), which transfers sialic acid preferably onto Gal-β-1,4-GlcNAc as an acceptor, and thus it was considered as a suitable candidate for the sialylation of the α-dystroglycan. The expressions of the three proteins were analyzed by real time-PCR and immunohistochemistry on modified methacarn fixed paraffin tissue sections of mouse skeletal muscle samples collected at days 0, 14 and 35 post infection. According to our findings, the up-regulation of GNE was a characteristic of the early and the late stage of the Nurse cell development. Additional features of this process were the elevated expressions of α-dystroglycan and the enzyme ST3Gal6. We provided strong evidence that an increased local synthesis of sialic acids is a trait of the Nurse cell of <i>T. spiralis</i>, and at least in part due to an overexpression of α-dystroglycan. In addition, circumstantially we suggest that the enzyme ST3Gal6 is engaged in the process of sialylation of the major oligosaccharide component of α-dystroglycan.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 3","pages":"217-225"},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/de/helm-59-217.PMC9831521.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10671898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ligula Intestinalis</i> Infection in a Native Leuciscid Hybrid (<i>Alburnus Derjugini</i> x <i>Squalius Orientalis</i>) in The Kürtün Dam Lake, Northeast Anatolia.","authors":"Z Z İpek,&nbsp;C Kaya,&nbsp;Ş Kayiş","doi":"10.2478/helm-2022-0008","DOIUrl":"https://doi.org/10.2478/helm-2022-0008","url":null,"abstract":"<p><p>Taxonomic evaluations are needed to accurately determine the host selection of fish parasites. The present study is a multidisciplinary research in the field of basic and fish diseases sciences. The description of the hybrid species of <i>Squalius orientalis</i> and <i>Alburnus derjugini</i> and infection of <i>Ligula intestinalis</i> in these hybrid fish were reported for the first time from the Kürtün Dam Lake in northeast Turkey. A total of 450 fish were sampled in March, August, and October in 2020 using gillnets. Detailed morphological characteristics (n = 24) were compared to determine the difference among ancestors and hybrid species. The prevalence of <i>L. intestinalis</i> between the sampling periods and the size groups of fish (0 - 10, 11 - 15, and ≥16 cm in length) were examined. Moreover, the highest prevalence of the parasite was observed in October (78.94 %), with a size range of 0 - 10 cm in length (77.8 %). In addition, the total prevalence of the parasite was 48.44 %. The results revealed that most of the diagnostic metric and meristic features of hybrid fish were ranging between the data of <i>S. orientalis</i> and <i>A. derjugini</i>. According to previous reports, when hybrid individuals were compared with their ancestors in terms of prevalence, hybrid individuals were more susceptible to <i>L. intestinalis</i> infections. This study was unique as it provided the first record of <i>L. intestinalis</i> in a hybrid fish population.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"83-93"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/ab/helm-59-083.PMC9075872.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10760654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Seasonal Variation on The Prevalence and Risk Factors of Trichuriasis Among the Aboriginal Community in Malaysia.","authors":"S A Noradilah,&nbsp;T S Anuar,&nbsp;I L Lee","doi":"10.2478/helm-2022-0004","DOIUrl":"https://doi.org/10.2478/helm-2022-0004","url":null,"abstract":"<p><p><i>Trichuris trichiura</i> is a soil-transmitted helminth prevalent in developing countries with poor, inadequate sanitation and unsafe water sources. In Malaysia, the prevalence of trichuriasis is relatively high among the aboriginal community due to poverty and poor sanitation. However, there are few studies to determine the seasonal variation on the prevalence and risk factors to acquire <i>Trichuris trichiura</i> infection in Malaysia. The present study found higher <i>Trichuris trichiura</i> infection during the dry season (63.6%; 138/217) in comparison to the wet season (55.5%; 142/256). Low household income, low education level and practice of open defecation were significant risk factors to acquire <i>Trichuris trichiura</i> infection during the wet season. Usage of untreated water supplies for daily activities was a risk factor to acquire trichuriasis during the dry season. This study highlighted that poverty and poor sanitation practices as well as drinking untreated water put the aborigines at high risk to acquire trichuriasis. Therefore, health education, improved sanitation and provision of treated water supply are recommended for the prevention and control of <i>Trichuris trichiura</i> infections in the aboriginal community.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"55-63"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8c/f7/helm-59-055.PMC9075873.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10386309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Data on Pipefishes' and Seahorse's Endohelminths Off Crimean Coasts of The Black Sea.","authors":"Y Kornyychuk,&nbsp;T Polyakova,&nbsp;N Pronkina","doi":"10.2478/helm-2022-0006","DOIUrl":"https://doi.org/10.2478/helm-2022-0006","url":null,"abstract":"<p><p>A total of 357 Syngnathidae fishes (<i>Syngnathus abaster</i> Risso, 1827, <i>S. typhle</i> Linnaeus, 1758 and <i>Hippocampus hippocampus</i> (Linnaeus, 1758) caught in different habitats along Crimean Black Sea shelf were examined for presence of endohelminths and revealed to be hosts of 15 helminth species. The fishes are second intermediate hosts for five \"birds'\" digenean species and nematodes (larvae of three species and immature adults of one more species) completing life-cycles in waterbirds and fish; for two acanthocephalans and three cestodes larvae ending development in fish. We suggest, basing on data on feeding of the Black Sea predatory fish and waterbirds, that Syngnathid fishes are paratenic hosts in parasitic systems of most cestodes, nematodes and acanthocephalans. All the trematodes found are generalists at metacercarial stage; specialists <i>Timoniella imbutiformis</i> and <i>Aphallus tubarium</i> use Syngnathidae as definitive hosts. Cestodes, nematodes as well as acanthocephalans found are generalists, too. Based on infection indices, <i>S. typhle</i> are main final host and <i>H. hippocampus</i> are main 2nd intermediate hosts for <i>T. imbutiformis</i>; Syngnathids are accidental hosts for other trematodes as well as for all the cestodes, nematodes and acanthocephalans. <i>Cryptocotyle concava</i> and <i>Pygidiopsis genata</i> are important as Syngnathid fishes' threats, especially in marine protected aquatoria, being potential agents of \"black spot disease\".</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"74-82"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/8e/helm-59-074.PMC9075879.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10743187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasite Community Analysis of The Gray Snapper <i>Lutjanus Griseus</i> (Perciformes,Lutjanidae) in a Tropical Region of The Southern Gulf of Mexico.","authors":"M A Rodríguez-Santiago,&nbsp;L Ramos-Colorado,&nbsp;L García-Magaña,&nbsp;M I Grano-Maldonado,&nbsp;J Iannacone,&nbsp;A Vázquez-Caballero","doi":"10.2478/helm-2022-0003","DOIUrl":"https://doi.org/10.2478/helm-2022-0003","url":null,"abstract":"<p><p>The gray snapper <i>Lutjanus griseus</i> is a commercially important fish species along its distribution range in the western Atlantic Ocean. However, despite its importance, there is still little knowledge about its parasitic fauna for the Mexican coasts of the Gulf of Mexico. The aims of this research were to generate a list of the parasitic fauna present in juvenile gray snapper <i>L. griseus</i> from a coastal lagoon located in southeastern Mexico, to evaluate the infection levels of parasites and to determine the relationship between the abundance of parasites and the fish size and condition factor. Samples of <i>L. griseus</i> (12 - 29.2 mm) were obtained in two periods of the year (dry and rainy seasons) to examine the intra-annual variability of its parasitic fauna. A total of 17 parasite species were recorded belonging to six taxonomic groups (Myxozoa, Monogenea, Digenea, Cestoda, Nematoda and Acanthocephala). The highest levels of infection (abundance, prevalence and intensity of infection) were found for the monogeneans <i>Euryhaliotrema griseus</i> and <i>Euryhaliotrema fastigatum</i>. There were no significant correlations between the total abundance of parasites and the fish condition and size (total length) in not any of the two seasons studied, suggesting that the body size and the biological condition index of the host did not directly influence the abundance of parasites in early life stages of <i>L. griseus</i>. Moreover, the species of parasites found that could be zoonotic for humans through the consumption of raw or inadequately cooked fish were the nematodes <i>Contracaecum</i> sp. type 1, <i>Contracaecum</i> sp. type 2, <i>Cucullanus pargi</i> and <i>Pseudoterranova</i> sp. The presence of the monogeneans <i>E. griseus</i> and <i>E. fastigatum</i> was also highlighted because these ectoparasite species are known to cause harm to fish under culture systems. All the parasite species found in this study, except nematodes, were new records of geographic distribution.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"94-103"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a1/a8/helm-59-094.PMC9075871.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10760650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic Changes of <i>Trichinella Spiralis</i> Treated By <i>Commiphora Molmol,Lepidium Sativum</i>, and Albendazole: <i>in Vitro</i> Study.","authors":"G L Abuelenain,&nbsp;Z H Fahmy,&nbsp;A M Elshennawy,&nbsp;E H A Selim,&nbsp;M Elhakeem,&nbsp;K M A Hassanein,&nbsp;S M Awad","doi":"10.2478/helm-2022-0005","DOIUrl":"https://doi.org/10.2478/helm-2022-0005","url":null,"abstract":"<p><p>Trichinellosis is a nematode-causing disease distinguished by its continuous transmission in the carnivores and omnivores. Despite effective eradication of the enteral forms, conventional drugs fail to eliminate the migrating and muscle ones. Over the past years, researchers intensified the work on herbal medicines as alternatives or aids to albendazole, the reference drug. This research hypothesizes that the therapeutic agent absorption route could be an evidence-based carrier molecule or auxiliary drug to albendazole. Accordingly, this <i>in vitro</i> study was designed to investigate mainly the phenotypic changes induced by a mono-treatment of albendazole, <i>Lipidium sativum</i> (garden cress), and <i>Commiphora molmol</i> (myrrh). Incredibly, no data were reported on the morphological alterations of <i>T. spiralis</i> larvae treated by any of these drugs. The experimental design tested various concentrations (25, 50, 100, and 200 μg/ml) of each herbal medicine for the lethal effects on the parasite forms for a day (1, 12, and 24h). The data showed that the highest significant mortality rate of the parasite forms was in favor of the concentration 200 μg/ml of both plant extracts in a time-dependent manner. Therefore, albendazole at 200 μg/ml dose was tested in parallel, and all experimental groups were compared to non-treated muscle larvae and worms. Albendazole-treated worms accounted for the least significant (<i>p<0.001</i>) survival rate (2 %), followed by myrrh (5 %), and the adverse was valid for the survival rate of the muscle larvae at that time. None of the larvae/worms was alive after 24 hours of incubation with the 200μg/ml of either treatment. The scanning electron microscope investigation of the experimental groups provided a shred of evidence for different routes of taking up the candidate drugs by the parasite. In conclusion, the results of the previous work <i>in vivo</i> and current <i>in vitro</i> study recommend myrrh over garden cress as a complementary agent of albendazole.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"37-45"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/8a/helm-59-037.PMC9075874.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10760648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of Intestinal Parasite Infections in Goats from Smallholder Farms in Northern Thailand.","authors":"K Wuthijaree,&nbsp;P Tatsapong,&nbsp;C Lambertz","doi":"10.2478/helm-2022-0007","DOIUrl":"https://doi.org/10.2478/helm-2022-0007","url":null,"abstract":"<p><p>The present study determined the prevalence of gastrointestinal (GI) parasites in small ruminants kept in smallholder farms in Phitsanulok, Northern Thailand. A total of 885 goats from 31 smallholder farms were selected randomly between May 2019 to January 2020. Fecal samples were collected to estimate fecal egg counts and oocysts counts using the modified McMaster technique. Furthermore, pooled fecal samples from each of the farms were cultured in order to differentiate third-stage larvae of nematode. Examination of fecal samples revealed that 885 were positive for one or more parasites, giving an overall prevalence of 87.2 %, of which 38.6 % were infected with one and 48.6 % with multiple parasite. <i>Strongyles</i> and <i>Eimeria</i> oocysts were the most prevalent. <i>Haemonchus contortus</i> and <i>Strongyloides papillosus</i> were the predominant species based on the percentage of larvae in fecal cultures. Tapeworm eggs were encountered in 14.2 % of all samples. No significant difference in gastrointestinal parasite prevalence was associated with sex of the host. Infection was significantly (P = 0.009) highest in poor body conditioned goats (72.0 %) as compared to moderate (48.9 %) and good body conditioned (50.0 %) goats. This report on prevalence of GI parasites of small ruminants in smallholder farms in Northern Thailand reveals a high endoparasitic infections that appeared well-adapted to environmental conditions. Further studies on endoparasite control are required to establish the impact of parasitism on productive performance, including monitoring control parasite programs are needed for better health and productivity.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"64-73"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/7e/helm-59-064.PMC9075877.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10394625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transmission Route Used By Parasitic Lasidium Larvae of The Freshwater Mussel <i>Anodontites Trapesialis</i> on Guppies <i>Poecilia Reticulata</i> During Short Cohabitation.","authors":"M I Grano-Maldonado,&nbsp;I B Madureira,&nbsp;N S S Trigueiro,&nbsp;N V Gonçalves,&nbsp;T L Rocha","doi":"10.2478/helm-2022-0009","DOIUrl":"https://doi.org/10.2478/helm-2022-0009","url":null,"abstract":"<p><p>We describe for the first time the transmission route employed by the parasitic larvae of the freshwater mussel <i>Anodontites trapesialis</i> (Lamarck, 1819) during cohabitation on the guppy <i>Poecilia reticulata</i> Peter, 1859. The freshwater mussel and fish-host were employed as a model system to investigate the infection and parasite establishment. Laboratory experiments included video recording and histopathological analysis of the infection. <i>In vivo</i> video observations demonstrated that lasidium larvae could be transmitted to guppies during direct contact. A series of histology samples and photography suggest that this larva attaches and colonizes possibly on-site on the outer surface of the fish, causing cellular inflammation in the epidermis layer with cellular hyperplasia in the zone of parasite attachment. An evident hyaline layer, cellular hypertrophy, and a large number of undifferentiated proliferating cells were observed. Hemorrhagic tissue and swelling were observed in the epidermis and dermic zone. The total number of larvae per male and female guppy was 525 ± 86 and 494 ± 167, respectively. No parasitic preference was detected for male versus female parasitized fish.</p>","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"59 1","pages":"104-110"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ed/68/helm-59-104.PMC9075875.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10394626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}