Aphelenchoides varicaudatus (Nematoda: Aphelenchoididae) and Helicotylenchus erythrinae (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia.

IF 1.1 4区生物学 Q4 PARASITOLOGY

Helminthologia Pub Date : 2023-06-04 eCollection Date: 2023-03-01 DOI:10.2478/helm-2023-0007

R Maharani, S Indarti, A Soffan, S Hartono

{"title":"Aphelenchoides varicaudatus (Nematoda: Aphelenchoididae) and Helicotylenchus erythrinae (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia.","authors":"R Maharani, S Indarti, A Soffan, S Hartono","doi":"10.2478/helm-2023-0007","DOIUrl":null,"url":null,"abstract":"There were two most found genera of plant parasitic nematodes from garlic plantation in Magelang, Central Java, Indonesia which suffered losses due to bulb rot, Aphelenchoides and Helicotylenchus. Polymerase Chain Reaction (PCR) was conducted using a pair of universal nematode primer (D2A/D3B) to determine the Aphelenchoides and Helicotylenchus species from those host. Both genera were amplified at ~780 bp. The Blast-N results for the Aphelenchoides showed high identity to Aphelenchoides varicaudatus from Yunnan China (HQ283353) with 99.47 % identity, while the Helicotylenchus showed 95.22 % identity to Helicotylenchus erythrinae from Colombia (MT321739). From morphological and molecular data, we confirm that the Aphelenchoides species is A. varicaudatus. Based on female morphological character, Helicotylenchus species refers to H. erythrinae. Which is also supported by its nucleotide alignment which has same region character as H. erythrinae (MT321739). This is the first report of molecular characterization of H. erythrinae in Indonesia.","PeriodicalId":55061,"journal":{"name":"Helminthologia","volume":"60 1","pages":"94-105"},"PeriodicalIF":1.1000,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/39/helm-60-1-helm-2023-0007.PMC10251759.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Helminthologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2478/helm-2023-0007","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/3/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

There were two most found genera of plant parasitic nematodes from garlic plantation in Magelang, Central Java, Indonesia which suffered losses due to bulb rot, Aphelenchoides and Helicotylenchus. Polymerase Chain Reaction (PCR) was conducted using a pair of universal nematode primer (D2A/D3B) to determine the Aphelenchoides and Helicotylenchus species from those host. Both genera were amplified at ~780 bp. The Blast-N results for the Aphelenchoides showed high identity to Aphelenchoides varicaudatus from Yunnan China (HQ283353) with 99.47 % identity, while the Helicotylenchus showed 95.22 % identity to Helicotylenchus erythrinae from Colombia (MT321739). From morphological and molecular data, we confirm that the Aphelenchoides species is A. varicaudatus. Based on female morphological character, Helicotylenchus species refers to H. erythrinae. Which is also supported by its nucleotide alignment which has same region character as H. erythrinae (MT321739). This is the first report of molecular characterization of H. erythrinae in Indonesia.

Abstract Image

查看原文本刊更多论文

印度尼西亚中爪哇马格朗大蒜种植园的 Aphelenchoides varicaudatus（线虫纲：Aphelenchoididae）和 Helicotylenchus erythrinae（线虫纲：Hoplolaimidae）。

在印度尼西亚中爪哇马格朗因球茎腐烂而遭受损失的大蒜种植园中，发现了两种最常见的植物寄生线虫属：Aphelenchoides 和 Helicotylenchus。使用一对通用线虫引物（D2A/D3B）进行聚合酶链式反应（PCR），以确定寄主中的 Aphelenchoides 和 Helicotylenchus 的种类。两个属的扩增片段均为 ~780 bp。Blast-N结果显示，Aphelenchoides与来自中国云南的Aphelenchoides varicaudatus（HQ283353）具有99.47%的一致性，而Helicotylenchus与来自哥伦比亚的Helicotylenchus erythrinae（MT321739）具有95.22%的一致性。根据形态学和分子数据，我们确认该 Aphelenchoides 为 A. varicaudatus。根据雌虫的形态特征，Helicotylenchus种属于H. erythrinae。其核苷酸比对结果也证实了这一点，核苷酸比对结果与 H. erythrinae (MT321739)具有相同的区域特征。这是在印度尼西亚首次报道赤潮黑螺菌的分子特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Helminthologia 生物-动物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Helminthologia (HELMIN), published continuously since 1959, is the only journal in Europe that encompasses the individual and collaborative efforts of scientists working on a different topics of human, veterinary and plant helminthology. The journal responsibility is to enrich the theoretical and practical knowledge in very specific areas and thus contribute to the advancements in human and veterinary medicine and agronomy. Taking the advantage of comprehensive and multidisciplinary approaches journal still maintains its original spirit and is principal source of fresh scientific information regarding helminths, endoparasites and plant parasites. Addressing the most up-to date topics journal gained rightful and exceptional place next to the other high-quality scientific journals publishing in its field.