Molecular characterization of Bertiella studeri infecting a primate in South India

IF 1.1 4区生物学 Q4 PARASITOLOGY

Helminthologia Pub Date : 2024-07-21 DOI:10.2478/helm-2024-0015

P. F. Sebasteena, C. K. Deepa, A. Varghese, K. G. Ajith Kumar, A. Joy, A. Iype, A. Rajappan, G. Chandy, R. Ravindran

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Abstract

Summary Bertiella spp. is a mite-borne cestode parasite that inhabits the small intestine of wide range of mammals, including non-human primates. In the present study, the morphological and molecular analysis of Bertiella studeri recovered from the small intestine of a bonnet macaque (Macaca radiata) from Wayanad, Kerala (South India) was performed. Acetic alum carmine staining identified the cestode morphologically based on the characters like broader proglottids, which contain irregularly alternating genital pores, single set of reproductive organs, 280 testes and a tubular transverse uterus. Molecular characterization was done using 18SrRNA, ITS1-5.8S and COX1 genes. Phylogenetic trees were constructed using MEGA X based on the Maximum Likelihood (ML) method (Hasegawa-Kishino-Yano (HKY) model). Cytochrome oxidase I gene could detect the existence of genetic variation in the parasite from two different hosts viz., monkey (Kerala, Argentina, and Kenya) and human (Sri Lanka). A minimum spanning network of haplotypes was generated by the haplotype networking with the above sequences using the popARTv1.7. Haplotype analysis based on COX1 revealed that the parasite haplotype was different in each country with highest population frequency in Sri Lanka.

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南印度一种灵长类动物感染的 Bertiella studeri 的分子特征

摘要 Bertiella spp.是一种螨媒绦虫寄生虫，栖息于包括非人灵长类动物在内的多种哺乳动物的小肠中。本研究对从喀拉拉邦（南印度）瓦尼亚德的倭帽猕猴（Macaca radiata）小肠中发现的 Bertiella studeri 进行了形态学和分子分析。醋矾胭脂红染色鉴定了该绦虫的形态特征，如较宽的前鞭毛，其中包含不规则交替的生殖孔，单套生殖器官，280 个睾丸和一个管状横向子宫。利用 18SrRNA、ITS1-5.8S 和 COX1 基因进行了分子鉴定。使用 MEGA X 基于最大似然法（ML）（长谷川-岸野（HKY）模型）构建了系统发生树。细胞色素氧化酶 I 基因可以检测到寄生虫在两种不同宿主（即猴子（喀拉拉邦、阿根廷和肯尼亚）和人类（斯里兰卡））中存在的遗传变异。基于 COX1 的单倍型分析表明，寄生虫的单倍型在每个国家都不同，其中斯里兰卡的群体频率最高。

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来源期刊

Helminthologia 生物-动物学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Helminthologia (HELMIN), published continuously since 1959, is the only journal in Europe that encompasses the individual and collaborative efforts of scientists working on a different topics of human, veterinary and plant helminthology. The journal responsibility is to enrich the theoretical and practical knowledge in very specific areas and thus contribute to the advancements in human and veterinary medicine and agronomy. Taking the advantage of comprehensive and multidisciplinary approaches journal still maintains its original spirit and is principal source of fresh scientific information regarding helminths, endoparasites and plant parasites. Addressing the most up-to date topics journal gained rightful and exceptional place next to the other high-quality scientific journals publishing in its field.

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