Hereditas最新文献_第8页

绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-08-12 DOI: 10.16288/J.YCZZ.15-160

李智方, 冯冲, 纪慧丽, 石宁宁, 宋小凤, 赵勤丽, 龙川, 潘登科, 杨小淦

{"title":"绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析","authors":"李智方, 冯冲, 纪慧丽, 石宁宁, 宋小凤, 赵勤丽, 龙川, 潘登科, 杨小淦","doi":"10.16288/J.YCZZ.15-160","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-160","url":null,"abstract":"The pig is an ideal source to provide organs because its organ size and physiology are similar to humans. However, an acute rejection will ensue after pig-to-human xenotransplantation. The α-1,3 galactosyltransferase gene knockout (GTKO) pigs were generated in recent years, and could solve the problem of hyperacute rejection. But due to lack of reporting genes, the rejection status of cells and organs post pig-to-human xenotransplantation cannot be visualized. In this study, we introduced the enhanced green fluorescent protein (EGFP) gene driven by the CAG promoter into GTKO porcine ear fibroblasts. Then we produced transgenic pigs expressing the EGFP gene by nuclear transfer technology. Expression levels of EGFP in different tissues and organs of the cloned pig were investigated by Nightsea DFP-1 Fluorescent Protein Flashlight, fluorescence microscope and quantitative PCR assays. The results showed that the protein and transcript of EGFP were expressed in all tissues and organs of the GTKO pig, but the expression was weak in the liver and central nervous system. In conclusion, we have successfully produced the transgenic GTKO pigs expressing EGFP in all tested tissues and organs, which builds up a good basis to track transplanted cells or tissues.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"37 1","pages":"1211-1217"},"PeriodicalIF":2.7,"publicationDate":"2015-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.16288/J.YCZZ.15-160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild Estonian and Russian sea trout (Salmo trutta) in Finnish coastal sea trout catches: results of genetic mixed-stock analysis 芬兰沿海捕捞的野生爱沙尼亚和俄罗斯海鳟(Salmo trutta):遗传混合种群分析的结果

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00070

Marja-Liisa Koljonen, Riho Gross, Jarmo Koskiniemi

{"title":"Wild Estonian and Russian sea trout (Salmo trutta) in Finnish coastal sea trout catches: results of genetic mixed-stock analysis","authors":"Marja-Liisa Koljonen, Riho Gross, Jarmo Koskiniemi","doi":"10.1111/hrd2.00070","DOIUrl":"10.1111/hrd2.00070","url":null,"abstract":"For responsible fisheries management of threatened species, it is essential to know the composition of catches and the extent to which fisheries exploit weak wild populations. The threatened Estonian, Finnish and Russian sea trout populations in the Gulf of Finland are targets of mixed-stock fisheries. The fish may originate from rivers with varying production capacities, from different countries, and they may also have either a wild or hatchery origin. In order to resolve the composition of Finnish coastal sea trout catches, we created a standardized baseline dataset of 15 DNA microsatellite loci for 59 sea trout populations around the Gulf of Finland and tested its resolution for mixed-stock analysis of 1372 captured fish. The baseline dataset provided sufficient resolution for reliable mixture analysis at regional group level, and also for most of the individual rivers stocks. The majority (76–80%) of the total catch originated from Finnish sea trout populations, 6–9% came from Russian and 12–15% from Estonian populations. Nearly all Finnish trout in the catch were of hatchery origin, while the Russian and Estonian trout were mostly of wild origin. The proportion of fish in the Finnish catches that originated from rivers with natural production was at least one fifth (22%, 19–23%). Two different spotting patterns were observed among the captured trout, with a small and sparsely spotted form being markedly more common among individuals of Russian (28%) and Estonian origin (22%) than among fish assigned to a Finnish origin (0.7%).","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"177-195"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32975145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat 一种改进的MALDI-TOF质谱方法和一种鉴定小麦过表达Bx7谷蛋白亚基的新型DNA标记

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00069

Ke Wang, Shahidul Islam, Junhong Ma, Masood Anwar, Jing Chen, Yueming Yan, Rudi Appels, Wujun Ma

引用次数: 4

Genetic polymorphism of 15 Y chromosomal STR loci and haplotypes of Henan Han population 河南汉族人群15个Y染色体STR位点的遗传多态性及单倍型分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00067

Xiu-Hua Zhang, Wen-Bo Zhang, Xue-Hui Fan

引用次数: 3

Deletion mapping in the Enhancer of split complex 分裂复合体增强子中的删除映射

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00065

Elisa Wurmbach, Anette Preiss

{"title":"Deletion mapping in the Enhancer of split complex","authors":"Elisa Wurmbach, Anette Preiss","doi":"10.1111/hrd2.00065","DOIUrl":"10.1111/hrd2.00065","url":null,"abstract":"The Enhancer of split complex [E(spl)-C] comprises twelve genes of different classes. Seven genes encode proteins of with a basic-helix-loop-helix-orange (bHLH-O) domain that function as transcriptional repressors and serve as effectors of the Notch signalling pathway. They have been named E(spl)m8-, m7-, m5-, m3-, mβ-, mγ- and mδ-HLH. Four genes, E(spl)m6-, m4-, m2- and mα-BFM are intermingled and encode Notch repressor proteins of the Bearded-family (BFM). The complex is split by a single gene of unrelated function, encoding a Kazal-type protease inhibitor (Kaz-m1). All members within a family, bHLH-O or BFM, are very similar in structure and in function. In an attempt to generate specific mutants, we have mobilised P-element constructs residing next to E(spl)m7-HLH and E(spl)mγ-HLH, respectively. The resulting deletions were mapped molecularly and by cytology. Two small deletions affected only E(spl)m7-HLH and E(spl)mδ. The deficient flies were viable without apparent phenotype. Larger deletions, generated also by X-ray mutagenesis, uncover most of the E(spl)-C. The phenotypes of homozygous deficient embryos were analysed to characterize the respective loss of Notch signalling activity.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"159-168"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32974124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Association analysis of the GRM8 gene with schizophrenia in the Uygur Chinese population 维吾尔族人群中GRM8基因与精神分裂症的相关性分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00045

Lili Zhang, Xianjiang Zhong, Zhiguo An, Shuxian Han, Xiao Luo, Yongyong Shi, Qizhong Yi

{"title":"Association analysis of the GRM8 gene with schizophrenia in the Uygur Chinese population","authors":"Lili Zhang, Xianjiang Zhong, Zhiguo An, Shuxian Han, Xiao Luo, Yongyong Shi, Qizhong Yi","doi":"10.1111/hrd2.00045","DOIUrl":"10.1111/hrd2.00045","url":null,"abstract":"GRM8 is a schizophrenia candidate gene that is also thought to be involved in the glutamate pathway, which is very important in the pathogenesis of schizophrenia. In this study, we aim to investigate the association between GRM8 and schizophrenia in the Uygur Chinese population.Rs2237748 and rs2299472, located in the GRM8 gene, were selected for genotyping in a set of Uygur Chinese case-control samples, which included 723 cases and 561 controls, using TaqMan assays and capillary sequencing. The statistical analysis was carried out using the online software program SHEsis, and a meta-analysis was carried out to identify other relevant studies using Review Manager 5. We found that the rs2299472 genotype was significantly associated with schizophrenia (P = 0.015, P = 0.030, after Bonferroni correction). The frequency of the CC genotype was higher in the schizophrenic patients (P = 0.008), and the frequency of the AC genotype was lower (P = 0.008). Furthermore, the meta-analysis incorporating the previous and current studies also showed that rs2299472 is associated with schizophrenia. This study indicates that the GRM8 gene may play an important role in the pathogenesis of schizophrenia.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"140-144"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32974122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless 人RITA与果蝇无毛抑制因子的相互作用分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00074

Birgit Brockmann, Helena Mastel, Franz Oswald, Dieter Maier

{"title":"Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless","authors":"Birgit Brockmann, Helena Mastel, Franz Oswald, Dieter Maier","doi":"10.1111/hrd2.00074","DOIUrl":"10.1111/hrd2.00074","url":null,"abstract":"Notch signalling mediates intercellular communication, which is effected by the transcription factor CSL, an acronym for vertebrate CBF1/RBP-J, Drosophila Suppressor of Hairless [Su(H)] and C. elegans Lag1. Nuclear import of CBF1/RBP-J depends on co-activators and co-repressors, whereas the export relies on RITA. RITA is a tubulin and CBF1/RBP-J binding protein acting as a negative regulator of Notch signalling in vertebrates. RITA protein is highly conserved in eumatazoa, but no Drosophila homologue was yet identified. In this work, the activity of human RITA in the fly was addressed. To this end, we generated transgenic flies that allow a tissue specific induction of human RITA, which was demonstrated by Western blotting and in fly tissues. Unexpectedly, overexpression of RITA during fly development had little phenotypic consequences, even when overexpressed simultaneously with either Su(H) or the Notch antagonist Hairless. We demonstrate the in vivo binding of human RITA to Su(H) and to tubulin by co-immune precipitation. Moreover, RITA and tubulin co-localized to some degree in several Drosophila tissues. Overall our data show that human RITA, albeit binding to Drosophila Su(H) and tubulin, cannot influence the Notch signalling pathway in the fly, suggesting that a nuclear export mechanism of Su(H), if existent in Drosophila, does not depend on RITA.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"209-219"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00074","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32975148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

A tale of two papers 两张纸的故事

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00073

Anssi Saura

引用次数: 3

Strange history: the fall of Rome explained in Hereditas 奇怪的历史:《赫里达斯传》解释了罗马的灭亡

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00080

Bengt O. Bengtsson

{"title":"Strange history: the fall of Rome explained in Hereditas","authors":"Bengt O. Bengtsson","doi":"10.1111/hrd2.00080","DOIUrl":"10.1111/hrd2.00080","url":null,"abstract":"In 1921 Hereditas published an article on the fall of Rome written by the famous classical scholar Martin P:son Nilsson. Why was a paper on this unexpected topic printed in the newly founded journal? To Nilsson, the demise of the Roman Empire was explained by the “bastardization” occurring between “races” from different parts of the realm. Offspring from mixed couples were of a less stable “type” than their parents, due to the breaking up by recombination of the original hereditary dispositions, which led to a general loss of competence to rule and govern. Thus, the “hardness” of human genes, together with their recombination, was – according to Nilsson – the main cause of the fall of Rome.Nilsson's argument is not particularly convincingly presented. Human “races” are taken to have the same genetic structure as inbred crop strains, and Nilsson believes in a metaphysical unity between the individual and the race to which it belongs. However, in my view, Martin P:son Nilsson and his friend Herman Nilsson-Ehle had wider aims with the article than to explain a historical event. The article can be read as indicating strong support from the classical human sciences to the ambitious new science of genetics. Support is also transferred from genetics to the conservative worldview, where the immutability and inflexibility of the Mendelian genes are used to strengthen the wish for greater stability in politics and life. The strange article in Hereditas can, thus, be read as an early instance in the – still ongoing – tug-of-war between the conservative and the liberal ideological poles over how genetic results best are socially interpreted.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"132-139"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32974121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genetic diversity and population structure of leafy kale and Brassica rupestris Raf. in south Italy 叶甘蓝和芸苔的遗传多样性和群体结构。在意大利南部

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00058

Lorenzo Maggioni, Roland von Bothmer, Gert Poulsen, Ferdinando Branca, Rikke Bagger Jørgensen

{"title":"Genetic diversity and population structure of leafy kale and Brassica rupestris Raf. in south Italy","authors":"Lorenzo Maggioni, Roland von Bothmer, Gert Poulsen, Ferdinando Branca, Rikke Bagger Jørgensen","doi":"10.1111/hrd2.00058","DOIUrl":"10.1111/hrd2.00058","url":null,"abstract":"Local varieties of leafy kales (Brassica oleracea L.) are grown in home gardens in Calabria and Sicily for self-consumption, in the same area where the wild relative Brassica rupestris Raf. also grows. With the use of AFLP markers, comparisons were made of the genetic diversity and population structure of ten wild and 22 cultivated populations, as well as of a hybrid population and of four commercial cultivars of different B. oleracea crops. The level of genetic diversity was higher in leafy kales than in wild populations and this diversity was mainly distributed within populations. Wild populations remained distinct from cultivated material. Additionally, most wild populations were distinctively isolated from each other. On the other hand, it was not possible to molecularly distinguish even geographically distant leafy kale populations from each other or from different B. oleracea crops. It was possible to detect inter-crossing between leafy kales and B. rupestris. Findings from this study illustrate the existing level of genetic diversity in the B. oleracea gene pool. Individual populations (either wild or leafy kales) with higher levels of genetic diversity have been identified and suggestions are given for an informed conservation strategy. Domestication hypotheses are also discussed.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":"151 6","pages":"145-158"},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32974123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19