Hereditas最新文献_第7页

[The role of long non-coding RNAs in hematologic malignancies]. [长链非编码rna在血液恶性肿瘤中的作用]。

IF 2.7 3区生物学

Hereditas Pub Date : 2015-11-01 DOI: 10.16288/J.YCZZ.15-261

H. Wanli, G. Ai

引用次数: 1

[Genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of Lampetra japonica]. [日本七鳃鳗粘膜免疫系统淋巴细胞样细胞免疫应答的遗传基础]。

IF 2.7 3区生物学

Hereditas Pub Date : 2015-11-01 DOI: 10.16288/j.yczz.15-108

Xin Liu, Xueying Song, Xiaoping Zhang, Ying-lun Han, Ting Zhu, R. Xiao, Qing-wei Li

{"title":"[Genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of Lampetra japonica].","authors":"Xin Liu, Xueying Song, Xiaoping Zhang, Ying-lun Han, Ting Zhu, R. Xiao, Qing-wei Li","doi":"10.16288/j.yczz.15-108","DOIUrl":"https://doi.org/10.16288/j.yczz.15-108","url":null,"abstract":"In recent years, the antigen recognition mechanism based on variable lymphocyte receptors (VLRs) was found in agnathan lamprey. To illuminate the genetic basis of immune response of lymphocyte-like cells in the mucosal immune system of lamprey and explore the evolutionary relationship of adaptive immune responses between the jawless and jawed vertebrates, we constructed cDNA libraries of lamprey (Lampetra japonica) gills before and after stimulation, and then performed high-throughput transcriptome sequencing and analysis. Through functional annotation of 88 525 assembled unigenes, 21 704 and 9769 unigenes were annotated in Gene Ontology (GO) and Kyto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. Among 999 unigenes involved in multiple pathways of immune system, 184 unigenes were highly homologous to 51 TCR (T cell receptor) and BCR (B cell receptor) signalling molecules in higher vertebrates, indicating that molecules involved in adaptive immune signalling pathways in higher vertebrates also exist in lampreys. In addition, identification of five VLRA, seven VLRB and four VLRC molecules suggest that at least three types of lymphocyte subsets are distributed in lamprey gill mucosal immune tissues. The results of real-time fluorescence quantitative PCR showed that the expression levels of Lck, Fyn and Zap70 were up-regulated after immune stimulation while those of Syk, Btk and Blnk were not changed significantly, indicating the activation of TCR-like signal transduction pathway after antigen stimulation in lamprey gill tissues. Our studies preliminaryly proved that two parallel adaptive immune systems in jawless and jawed vertebrates have common genetic basis, and also provided valuable clues to the exploration of signalling processes of VLRA⁺, VLRB⁺, and VLRC⁺ lymphocyte-like cells in response to antigens.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Resurrection of Hereditas, a journal with almost 100 years of tradition 《赫里达斯的复活》一本有近百年历史的杂志

IF 2.7 3区生物学

Hereditas Pub Date : 2015-10-22 DOI: 10.1186/s41065-015-0003-8

S. Baumgartner

引用次数: 1

Analysis of genetic diversity of Tunisian caprifig (Ficus carica L.) accessions using simple sequence repeat (SSR) markers 利用SSR标记分析突尼斯无花果(Ficus carica L.)遗传多样性

IF 2.7 3区生物学

Hereditas Pub Date : 2015-10-22 DOI: 10.1186/s41065-015-0002-9

Awatef Essid, F. Aljane, A. Ferchichi, J. Hormaza

引用次数: 29

The Trp719Arg polymorphism of the KIF6 gene and coronary heart disease risk: systematic review and meta-analysis KIF6基因Trp719Arg多态性与冠心病风险:系统回顾和荟萃分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-10-22 DOI: 10.1186/s41065-015-0004-7

David Ruiz-Ramos, Yazmín Hernández-Díaz, C. Tovilla-Zárate, I. Juárez-Rojop, M. López-Narváez, T. González-Castro, M. E. Torres-Hernández, M. Baños-González

引用次数: 3

Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29 棉花三螺旋转录因子GhGT29的克隆及功能分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-09-28 DOI: 10.16288/J.YCZZ.15-320

L. Yue, L. Xiaodong, D. Yongmei, X. Zongming, Shou-yi Chen

{"title":"Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29","authors":"L. Yue, L. Xiaodong, D. Yongmei, X. Zongming, Shou-yi Chen","doi":"10.16288/J.YCZZ.15-320","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-320","url":null,"abstract":"Trihelix transcription factors are important proteins involved in response to abiotic stresses in plants. Understanding the molecular mechanisms of Trihelix in cottons will lay the foundation to improve stress tolerance by gene engineering. In this study, a gene encoding Trihelix transcription factor was isolated in upland cottons using reverse transcription PCR according to bioinformatic analysis. The gene was named as GhGT29 (GenBank accession No. JQ013097), which was 1 092 bp, contained a 1 089 bp open reading frame and encoded a protein of 363 amino acids with a predicted molecular weight of 40.9 kDa and a isoelectric point of 5.45. SMART analysis showed GhGT29 contained one typical SANT motif. Phylogenetic analysis showed that GhGT29 belonged to the SH4 subfamily of the Trihelix family and was most closely related to AtSH4-like1 and AtSH4-like2. Quantitative real-time PCR (qRT-PCR) analysis revealed that GhGT29 was induced by high salt, drought, cold and abscisic acid. The expression profile also revealed that GhGT29 was constitutively expressed in all tested tissues, such as roots, stems, leaves, flowers, ovules (0 DPA) and fibers (12 DPA). The expression level of GhGT29 was the highest in flowers and the lowest in stems. Using the Arabidopsis protoplasts assay system, we found that the GhGT29 protein was located in cell nuclei and had trans-activation activity. These results revealed that GhGT29 might be involved in the regulation of stress resistance-related genes in stress signaling pathways in upland cottons.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2015-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-08-12 DOI: 10.16288/J.YCZZ.15-160

李智方, 冯冲, 纪慧丽, 石宁宁, 宋小凤, 赵勤丽, 龙川, 潘登科, 杨小淦

{"title":"绿色荧光蛋白在α-1,3半乳糖基转移酶敲除猪组织器官的表达分析","authors":"李智方, 冯冲, 纪慧丽, 石宁宁, 宋小凤, 赵勤丽, 龙川, 潘登科, 杨小淦","doi":"10.16288/J.YCZZ.15-160","DOIUrl":"https://doi.org/10.16288/J.YCZZ.15-160","url":null,"abstract":"The pig is an ideal source to provide organs because its organ size and physiology are similar to humans. However, an acute rejection will ensue after pig-to-human xenotransplantation. The α-1,3 galactosyltransferase gene knockout (GTKO) pigs were generated in recent years, and could solve the problem of hyperacute rejection. But due to lack of reporting genes, the rejection status of cells and organs post pig-to-human xenotransplantation cannot be visualized. In this study, we introduced the enhanced green fluorescent protein (EGFP) gene driven by the CAG promoter into GTKO porcine ear fibroblasts. Then we produced transgenic pigs expressing the EGFP gene by nuclear transfer technology. Expression levels of EGFP in different tissues and organs of the cloned pig were investigated by Nightsea DFP-1 Fluorescent Protein Flashlight, fluorescence microscope and quantitative PCR assays. The results showed that the protein and transcript of EGFP were expressed in all tissues and organs of the GTKO pig, but the expression was weak in the liver and central nervous system. In conclusion, we have successfully produced the transgenic GTKO pigs expressing EGFP in all tested tissues and organs, which builds up a good basis to track transplanted cells or tissues.","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2015-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.16288/J.YCZZ.15-160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67512505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild Estonian and Russian sea trout (Salmo trutta) in Finnish coastal sea trout catches: results of genetic mixed-stock analysis 芬兰沿海捕捞的野生爱沙尼亚和俄罗斯海鳟(Salmo trutta):遗传混合种群分析的结果

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00070

Marja-Liisa Koljonen, Riho Gross, Jarmo Koskiniemi

{"title":"Wild Estonian and Russian sea trout (Salmo trutta) in Finnish coastal sea trout catches: results of genetic mixed-stock analysis","authors":"Marja-Liisa Koljonen, Riho Gross, Jarmo Koskiniemi","doi":"10.1111/hrd2.00070","DOIUrl":"10.1111/hrd2.00070","url":null,"abstract":"<p>For responsible fisheries management of threatened species, it is essential to know the composition of catches and the extent to which fisheries exploit weak wild populations. The threatened Estonian, Finnish and Russian sea trout populations in the Gulf of Finland are targets of mixed-stock fisheries. The fish may originate from rivers with varying production capacities, from different countries, and they may also have either a wild or hatchery origin. In order to resolve the composition of Finnish coastal sea trout catches, we created a standardized baseline dataset of 15 DNA microsatellite loci for 59 sea trout populations around the Gulf of Finland and tested its resolution for mixed-stock analysis of 1372 captured fish. The baseline dataset provided sufficient resolution for reliable mixture analysis at regional group level, and also for most of the individual rivers stocks. The majority (76–80%) of the total catch originated from Finnish sea trout populations, 6–9% came from Russian and 12–15% from Estonian populations. Nearly all Finnish trout in the catch were of hatchery origin, while the Russian and Estonian trout were mostly of wild origin. The proportion of fish in the Finnish catches that originated from rivers with natural production was at least one fifth (22%, 19–23%). Two different spotting patterns were observed among the captured trout, with a small and sparsely spotted form being markedly more common among individuals of Russian (28%) and Estonian origin (22%) than among fish assigned to a Finnish origin (0.7%).</p>","PeriodicalId":55057,"journal":{"name":"Hereditas","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/hrd2.00070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32975145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat 一种改进的MALDI-TOF质谱方法和一种鉴定小麦过表达Bx7谷蛋白亚基的新型DNA标记

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00069

Ke Wang, Shahidul Islam, Junhong Ma, Masood Anwar, Jing Chen, Yueming Yan, Rudi Appels, Wujun Ma

引用次数: 4

Genetic polymorphism of 15 Y chromosomal STR loci and haplotypes of Henan Han population 河南汉族人群15个Y染色体STR位点的遗传多态性及单倍型分析

IF 2.7 3区生物学

Hereditas Pub Date : 2015-01-14 DOI: 10.1111/hrd2.00067

Xiu-Hua Zhang, Wen-Bo Zhang, Xue-Hui Fan

引用次数: 3