{"title":"Phosphorus acquisition, translocation, and redistribution in maize.","authors":"Hui-Ling Guo, Meng-Zhi Tian, Xian Ri, Yi-Fang Chen","doi":"10.1016/j.jgg.2024.09.018","DOIUrl":"10.1016/j.jgg.2024.09.018","url":null,"abstract":"<p><p>Phosphorus (P) is an essential nutrient for crop growth, making it important for maintaining food security as the global population continues to increase. Plants acquire P primarily via the uptake of inorganic phosphate (Pi) in soil through their roots. Pi, which is usually sequestered in soils, is not easily absorbed by plants and represses plant growth. Plants have developed a series of mechanisms to cope with P deficiency. Moreover, P fertilizer applications are critical for maximizing crop yield. Maize is a major cereal crop cultivated worldwide. Increasing its P-use efficiency is important for optimizing maize production. Over the past two decades, considerable progresses have been achieved in studies aimed at adapting maize varieties to changes in environmental P supply. Here, we present an overview of the morphological, physiological, and molecular mechanisms involved in P acquisition, translocation, and redistribution in maize and combine the advances in Arabidopsis and rice, to better elucidate the progress of P nutrition. Additionally, we summarize the correlation between P and abiotic stress responses. Clarifying the mechanisms relevant to improving P absorption and use in maize can guide future research on sustainable agriculture.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"287-296"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mingliang Zhang, Yuancong Wang, Qi Wu, Yangming Sun, Chenxu Zhao, Min Ge, Ling Zhou, Tifu Zhang, Wei Zhang, Yiliang Qian, Long Ruan, Han Zhao
{"title":"Time-course transcriptomic analysis reveals transcription factors involved in modulating nitrogen sensibility in maize.","authors":"Mingliang Zhang, Yuancong Wang, Qi Wu, Yangming Sun, Chenxu Zhao, Min Ge, Ling Zhou, Tifu Zhang, Wei Zhang, Yiliang Qian, Long Ruan, Han Zhao","doi":"10.1016/j.jgg.2024.09.021","DOIUrl":"10.1016/j.jgg.2024.09.021","url":null,"abstract":"<p><p>Nitrogen (N) serves both as a vital macronutrient and a signaling molecule for plants. Unveiling key regulators involved in N metabolism helps dissect the mechanisms underlying N metabolism, which is essential for developing maize with high N use efficiency. Two maize lines, B73 and Ki11, show differential chlorate and low-N tolerance. Time-course transcriptomic analysis reveals that the expression of N utilization genes (NUGs) in B73 and Ki11 have distinct responsive patterns to nitrate variation. By the coexpression networks, significant differences in the number of N response modules and regulatory networks of transcription factors (TFs) are revealed between B73 and Ki11. There are 23 unique TFs in B73 and 41 unique TFs in Ki11. MADS26 is a unique TF in the B73 N response network, with different expression levels and N response patterns in B73 and Ki11. Overexpression of MADS26 enhances the sensitivity to chlorate and the utilization of nitrate in maize, at least partially explaining the differential chlorate tolerance and low-N sensitivity between B73 and Ki11. The findings in this work provide unique insights and promising candidates for maize breeding to reduce unnecessary N overuse.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"400-410"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142481283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An LRR-RLK protein modulates drought- and salt-stress responses in maize.","authors":"Zhirui Yang, Chen Wang, Tengfei Zhu, Jiafan He, Yijie Wang, Shiping Yang, Yu Liu, Bochen Zhao, Chaohui Zhu, Shuqing Ye, Limei Chen, Shengxue Liu, Feng Qin","doi":"10.1016/j.jgg.2024.10.016","DOIUrl":"10.1016/j.jgg.2024.10.016","url":null,"abstract":"<p><p>Maize (Zea mays), which is a vital source of food, feed, and energy feedstock globally, has significant potential for higher yields. However, environmental stress conditions, including drought and salt stress, severely restrict maize plant growth and development, leading to great yield losses. Leucine-rich repeat receptor-like kinases (LRR-RLKs) function in biotic and abiotic stress responses in the model plant Arabidopsis (Arabidopsis thaliana), but their roles in abiotic stress responses in maize are not entirely understood. In this study, we determine that the LRR-RLK ZmMIK2, a homolog of the Arabidopsis LRR-RK MALE DISCOVERER 1 (MDIS1)-INTERACTING RECEPTOR LIKE KINASE 2 (MIK2), functions in resistance to both drought and salt stress in maize. Zmmik2 plants exhibit enhanced resistance to both stresses, whereas overexpressing ZmMIK2 confers the opposite phenotypes. Furthermore, we identify C2-DOMAIN-CONTAINING PROTEIN 1 (ZmC2DP1), which interacts with the intracellular region of ZmMIK2. Notably, that region of ZmMIK2 mediates the phosphorylation of ZmC2DP1, likely by increasing its stability. Both ZmMIK2 and ZmC2DP1 are mainly expressed in roots. As with ZmMIK2, knockout of ZmC2DP1 enhances resistance to both drought and salt stress. We conclude that ZmMIK2-ZmC2DP1 acts as a negative regulatory module in maize drought- and salt-stress responses.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"388-399"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142640297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The maize mTERF18 regulates transcriptional termination of the mitochondrial nad6 gene and is essential for kernel development.","authors":"Zhengwei Guan, Yong Wang, Jun Yang","doi":"10.1016/j.jgg.2025.01.001","DOIUrl":"10.1016/j.jgg.2025.01.001","url":null,"abstract":"<p><p>Mitochondria are semi-autonomous organelles present in eukaryotic cells, containing their own genome and transcriptional machinery. However, their functions are intricately linked to proteins encoded by the nuclear genome. Mitochondrial transcription termination factors (mTERFs) are nucleic acid-binding proteins involved in RNA splicing and transcription termination within plant mitochondria and chloroplasts. Despite their recognized importance, the specific roles of mTERF proteins in maize remain largely unexplored. Here, we clone and functionally characterize the maize mTERF18 gene. Our findings reveal that mTERF18 mutations lead to severely undifferentiated embryos, resulting in abortive phenotypes. Early kernel exhibits abnormal basal endosperm transfer layer and a significant reduction in both starch and protein accumulation in mterf18. We identify the mTERF18 gene through mapping-based cloning and validate this gene through allelic tests. mTERF18 is widely expressed across various maize tissues and encodes a highly conserved mitochondrial protein. Transcriptome data reveal that mTERF18 mutations disrupt transcriptional termination of the nad6 gene, leading to undetectable levels of Nad6 protein and reduced complex I assembly and activity. Furthermore, transmission electron microscopy observation of mterf18 endosperm uncover severe mitochondrial defects. Collectively, these findings highlight the critical role of mTERF18 in mitochondrial gene transcription termination and its pivotal impact on maize kernel development.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"422-431"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142973389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bin Li, Wenhao Liu, Jie Xu, Xuxu Huang, Long Yang, Fang Xu
{"title":"Decoding maize meristems maintenance and differentiation: integrating single-cell and spatial omics.","authors":"Bin Li, Wenhao Liu, Jie Xu, Xuxu Huang, Long Yang, Fang Xu","doi":"10.1016/j.jgg.2025.01.012","DOIUrl":"10.1016/j.jgg.2025.01.012","url":null,"abstract":"<p><p>All plant organs are derived from stem cell-containing meristems. In maize, the shoot apical meristem (SAM) is responsible for generating all above-ground structures, including the male and female inflorescence meristems (IMs), which give rise to tassel and ear, respectively. Forward and reverse genetic studies on maize meristem mutants have driven forward our fundamental understanding of meristem maintenance and differentiation mechanisms. However, the high genetic redundancy of the maize genome has impeded progress in functional genomics. This review comprehensively summarizes recent advancements in understanding maize meristem development, with a focus on the integration of single-cell and spatial technologies. We discuss the mechanisms governing stem cell maintenance and differentiation in SAM and IM, emphasizing the roles of gene regulatory networks, hormonal pathways, and cellular omics insights into stress responses and adaptation. Future directions include cross-species comparisons, multi-omics integration, and the application of these technologies to precision breeding and stress adaptation research, with the ultimate goal of translating our understanding of meristem into the development of higher yield varieties.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"319-333"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yumin Zhang, Sihan Zhen, Chunxia Zhang, Jie Zhang, Xiaoqing Shangguan, Jiawen Lu, Qingyu Wu, Lynnette M A Dirk, A Bruce Downie, Guoying Wang, Tianyong Zhao, Junjie Fu
{"title":"Natural variation of CT2 affects the embryo/kernel weight ratio in maize.","authors":"Yumin Zhang, Sihan Zhen, Chunxia Zhang, Jie Zhang, Xiaoqing Shangguan, Jiawen Lu, Qingyu Wu, Lynnette M A Dirk, A Bruce Downie, Guoying Wang, Tianyong Zhao, Junjie Fu","doi":"10.1016/j.jgg.2024.09.012","DOIUrl":"10.1016/j.jgg.2024.09.012","url":null,"abstract":"<p><p>Embryo size is a critical trait determining not only grain yield but also the nutrition of the maize kernel. Up to the present, only a few genes have been characterized affecting the maize embryo/kernel ratio. Here, we identify 63 genes significantly associated with maize embryo/kernel weight ratio using a genome-wide association study (GWAS). The peak GWAS signal shows that the natural variation in Zea mays COMPACT PLANT2 (CT2), encoding the heterotrimeric G protein α subunit, is significantly associated with the Embryo/Kernel Weight Ratio (EKWR). Further analyses show that a missense mutation of CT2 increases its enzyme activity and associates with EKWR. The function of CT2 on affecting embryo/kernel weight ratio is further validated by the characterization of two ct2 mutants, for which EKWR is significantly decreased. Subsequently, the key downstream genes of CT2 are identified by combining the differential expression analysis of the ct2 mutant and quantitative trait transcript analysis in the GWAS population. In addition, the allele frequency spectrum shows that CT2 was under selective pressure during maize domestication. This study provides important genetic insights into the natural variation of maize embryo/kernel weight ratio, which could be applied in future maize breeding programs to improve grain yield and nutritional content.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"432-440"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kangqi Wang, Ziqi Wu, Man Zhang, Xueyao Lu, Jinsheng Lai, Meiling Zhang, Yi Wang
{"title":"Metal ion transport in maize: survival in a variable stress environment.","authors":"Kangqi Wang, Ziqi Wu, Man Zhang, Xueyao Lu, Jinsheng Lai, Meiling Zhang, Yi Wang","doi":"10.1016/j.jgg.2025.01.005","DOIUrl":"10.1016/j.jgg.2025.01.005","url":null,"abstract":"<p><p>Maize (Zea mays) is the most widely cultivated crop in the world. Maize production is closely linked to the extensive uptake and utilization of various mineral nutrients. Potassium (K), calcium (Ca), and magnesium (Mg) are essential metallic macronutrients for plant growth and development. Sodium (Na) is an essential micronutrient for some C<sub>4</sub> and CAM plants. Several metallic micronutrients like iron (Fe), manganese (Mn), and zinc (Zn) serve as enzyme components or co-factors in plant cells. Maize has to face the combined ion stress conditions in the natural environment. The limited availability of these nutrients in soils restricts maize production. In saline land, excessive Na could inhibit the uptake of mineral elements. Additionally, aluminum (Al) and heavy metals cadmium (Cd) and lead (Pb) in soils are toxic to maize and pose a threat to food security. Thus, plants must evolve complex mechanisms to increase nutrient uptake and utilization while restraining harmful elements. This review summarizes the research progress on the uptake and transport of metal ions in maize, highlights the regulation mechanism of metal ion transporters under stress conditions, and discusses the future challenges for the improvement of maize with high nutrient utilization efficiency (NUE).</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"297-306"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoyan Liang, Pan Yin, Fenrong Li, Yibo Cao, Caifu Jiang
{"title":"ZmGolS1 underlies natural variation of raffinose content and salt tolerance in maize.","authors":"Xiaoyan Liang, Pan Yin, Fenrong Li, Yibo Cao, Caifu Jiang","doi":"10.1016/j.jgg.2024.12.013","DOIUrl":"10.1016/j.jgg.2024.12.013","url":null,"abstract":"<p><p>Salt stress significantly inhibits crop growth and development, and mitigating this can enhance salt tolerance in various crops. Previous studies have shown that regulating saccharide biosynthesis is a key aspect of plant salt tolerance; however, the underlying molecular mechanisms remain largely unexplored. In this study, we demonstrate that overexpression of a salt-inducible galactinol synthase gene, ZmGolS1, alleviates salt-induced growth inhibition, likely by promoting raffinose synthesis. Additionally, we show that natural variation in ZmGolS1 transcript levels contributes to the diversity of raffinose content and salt tolerance in maize. We further reveal that ZmRR18, a type-B response regulator transcription factor, binds to the AATC element in the promoter of ZmGolS1, with this binding increases the transcript levels of ZmGolS1 under salt conditions. Moreover, a single nucleotide polymorphism (termed SNP-302T) within the ZmGolS1 promoter significantly reduces its binding affinity for ZmRR18, resulting in decreased ZmGolS1 expression and diminished raffinose content, ultimately leading to a salt-hypersensitive phenotype. Collectively, our findings reveal the molecular mechanisms by which the ZmRR18-ZmGolS1 module enhances raffinose biosynthesis, thereby promoting maize growth under salt conditions. This research provides important insights into salt tolerance mechanisms associated with saccharide biosynthesis and identifies valuable genetic loci for breeding salt-tolerant maize varieties.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"346-355"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Liu, Boming Yang, Xunji Chen, Tengfei Zhang, Huairen Zhang, Yimo Du, Qian Zhao, Zhaogui Zhang, Darun Cai, Juan Liu, Huabang Chen, Li Zhao
{"title":"ZmL75 is required for colonization by arbuscular mycorrhizal fungi and for saline-alkali tolerance in maize.","authors":"Jie Liu, Boming Yang, Xunji Chen, Tengfei Zhang, Huairen Zhang, Yimo Du, Qian Zhao, Zhaogui Zhang, Darun Cai, Juan Liu, Huabang Chen, Li Zhao","doi":"10.1016/j.jgg.2024.12.015","DOIUrl":"10.1016/j.jgg.2024.12.015","url":null,"abstract":"<p><p>Saline-alkali soil severely reduces the productivity of crops, including maize (Zea mays). Although several genes associated with saline-alkali tolerance have been identified in maize, the underlying regulatory mechanism remains elusive. Here, we report a direct link between colonization by arbuscular mycorrhizal fungi (AMF) and saline-alkali tolerance in maize. We identify s75, a natural maize mutant that cannot survive under moderate saline-alkali soil conditions or establish AM symbioses. The saline-alkali hypersensitive phenotype of s75 is caused by a 1340-bp deletion in Zm00001d033915, designated as ZmL75. This gene encodes a glycerol-3-phosphate acyltransferase localized in the endoplasmic reticulum, and is responsible for AMF colonization. ZmL75 expression levels in roots correspond with the root length colonization (RLC) rate during early vegetative development. Notably, the s75 mutant line shows a complete loss of AMF colonization, along with alterations in the diversity and structure of its root fungal microbiota. Conversely, overexpression of ZmL75 increases the RLC rate and enhances tolerance to saline-alkali soil conditions. These results suggest that ZmL75 is required for symbiosis with AMF, which directly improves saline-alkali tolerance. Our findings provide insights into maize-AMF interactions and offer a potential strategy for maize improvement.</p>","PeriodicalId":54825,"journal":{"name":"Journal of Genetics and Genomics","volume":" ","pages":"334-345"},"PeriodicalIF":6.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}