Journal of Toxicology and Environmental Health-Part A-Current Issues最新文献

{"title":"Cocoa (<i>Theobroma cacao</i> L.) photobiological effect on human fibroblast cells (HFF-1) exposed to ultraviolet B (UVB) radiation.","authors":"Josiéle da Silva Prade, Camila Medianeira da Silva D'Ávila, Thayline Correia da Silva, Altevir Rossato Viana, André Passaglia Schuch, Isadora Cassel Livinalli, Ana Clara Zanini Bertoncelli, Fernanda Krapf Saccol, Tallys de Oliveira Mendes, Jean Lucas Gutknecht da Silva, Daniela Bitencourt Rosa Leal, Natielen Jacques Schuch, Gabriela Hass de Mello, Mariana Fernandes Ribeiro, Patrícia Gomes, Francine Carla Cadoná","doi":"10.1080/15287394.2024.2439535","DOIUrl":"https://doi.org/10.1080/15287394.2024.2439535","url":null,"abstract":"<p><p>This study aimed to investigate the <i>in vitro</i> photobiological action of cocoa solution on a human fibroblast cell line (HFF-1) exposed to ultraviolet B (UVB) radiation. Three experimental models were utilized, where fibroblast cells were treated with different concentrations of cocoa as follows: 50; 100; 250; 500; 750; 1000 or 1500 µg/ml and concomitantly exposed to UVB 7 kJ/m² for 10 min. The following parameters were examined 1) analysis of the pre-treatment action of cocoa; 2) investigation of the co-treatment activity of cocoa at the time of exposure; and 3) study the effect of cocoa in the post-treatment of the damage initiated by UVB. Cocoa exhibited biological action only in the post-treatment model at almost all tested concentrations compared to cells exposed to UVB alone. Further, fibroblast cells treated only with cocoa displayed higher levels of proliferation as evidenced by a decrease in the levels of basal reactive oxygen species (ROS) and nitric oxide (NO). In addition, cocoa also modulated the cell cycle of cells in the absence of UVB exposure by increasing the G2/M phase, although this did not generate significant changes in cells exposed concomitantly to cocoa and UVB. Therefore, data demonstrated that exposure to cocoa improved fibroblast cell-based control rates and exhibited an important reparative activity against damage initiated by UVB in human fibroblasts. Cocoa may thus be considered as a potential beneficial agent to be utilized in UVB-damaged skin cells.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"1-15"},"PeriodicalIF":2.3,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of bark extracts from <i>Macaranga denticulata</i> on renal fibrosis in streptozotocin-induced diabetic rats.","authors":"Sreevarsha Gali, Amit Kundu, Swati Sharma, Mee-Young Ahn, Zothan Puia, Vikas Kumar, In Su Kim, Jeong Hwan Kwak, Partha Palit, Hyung Sik Kim","doi":"10.1080/15287394.2024.2394586","DOIUrl":"10.1080/15287394.2024.2394586","url":null,"abstract":"<p><p><i>Macaranga denticulata</i> (MD) bark is commonly utilized in traditional medicine for diabetes prevention and treatment. The bark extract of MD is rich in prenyl or farnesyl flavonoids and stilbenes, which possess antioxidant properties. Although data suggest the potential therapeutic benefits of the use of MD in treating diabetic nephropathy (DN), the precise mechanisms underlying MD-initiated protective effects against DN are not well understood. This study aimed to assess the renoprotective properties of MD extract by examining renofibrosis inhibition, oxidative stress, and inflammation utilizing streptozotocin-induced DN male Sprague - Dawley rats. Diabetic rats were intraperitoneally injected with streptozotocin (STZ) to induce diabetes. After 6 days, these rats were orally administered MD extract (200 mg/kg/day) or metformin (200 mg/kg/day) for 14 days. The administration of MD extract significantly lowered blood glucose levels, restored body weight, and reduced urine levels of various biomarkers associated with kidney functions. Histopathological analysis revealed protective effects in both kidneys and pancreas. Further, MD extract significantly restored abnormalities in advanced glycation end products, oxidative stress biomarkers, and proinflammatory cytokine levels in STZ-treated rats. MD extract markedly reduced renal fibrosis biomarker levels, indicating recovery from renal injury, and reversed dysregulation of sirtuins and claudin-1 in the kidneys of rats with STZ-induced diabetes. In conclusion, data demonstrated the renoprotective role of MD extract, indicating plant extract's ability to suppress oxidative stress and regulate proinflammatory pathways during pathological changes in diabetic nephropathy.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"911-933"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142301234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity, chemical, and nutritional profile evaluation of biomass extracts of the <i>Lemna aequinoctialis</i> (duckweed) aquatic plant.","authors":"Natalia Nati, Iasmini Nicoli Galter, Iara Souza Costa, Emily Fabre Garcia, Gabriel Amorim Lopes, Hildegardo Seibert França, Levi Pompermayer Machado, Regildo Márcio Gonçalves da Silva, Silvia Tamie Matsumoto","doi":"10.1080/15287394.2024.2397643","DOIUrl":"10.1080/15287394.2024.2397643","url":null,"abstract":"<p><p><i>Lemna aequinoctialis</i> (duckweed) is the smallest and fast-growing aquatic plant species producing protein-rich biomass with high protein nutritional value, phytoremediation capacity, and nutrient removal from wastewater. Duckweed may also be used as a new potential bioreactor for biological products, such as vaccines, antibodies, and pharmaceutical proteins. Based upon the potential importanc of <i>L. aequinoctialis</i> in phytoremediation and as a bioreactor the aim of this study was to (1) characterize the chemical and nutritional profiles of <i>L. aequinoctialis</i> biomass utilizing an integrated multi-trophic aquaculture system (IMTA) and a pond, and (2) investigate the cytotoxic potential of different concentrations of organic extracts and fractions using the MTT bioassay. EDXRF and ICP-MS analyses indicated the presence of trace elements in lower amounts in relation to the biomass of <i>L. aequinoctialis</i> in the lagoon, emphasizing the importance of plant inclusion management to reduce bioaccumulation of these elements. Analysis of mineral profiles, fatty acids, and amino acids indicated a satisfactory nutritional composition for the use of biomass as a bioproduct. Pigment analysis showed a high concentration of carotenoids, especially astaxanthin. After standardizing the controls, the MTT cell viability test was carried out utilizing rat hepatoma cell line (HTC), which are metabolizing cells that were treated with aqueous or ethanolic extracts and the dichloromethane, ethyl acetate, and methanol fractions at different concentrations. No apparent cytotoxic potential was observed following treatments, since there was no significant reduction in cell viability. Therefore, this study provides information regarding the biomass of <i>L. aequinoctialis</i> derived from the IMTA system, which might support further research into the application of this species as a bioproduct.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"934-952"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity potential of a pyraclostrobin-based fungicide in plant and green microalgae models.","authors":"Tamires de Freitas Oliveira, Maria Fernanda Barbosa Vaz da Costa, Tamara Alessandra Costa Santos, Maria José Dos Santos Wisniewski, Larissa Fonseca Andrade-Vieira","doi":"10.1080/15287394.2024.2403131","DOIUrl":"10.1080/15287394.2024.2403131","url":null,"abstract":"<p><p>Pyraclostrobin-based fungicides play an effective role in controlling fungal diseases and are extensively used in agriculture. However, there is concern regarding the potential adverse effects attributed to exposure to these fungicides on non-target organisms and consequent influence exerted on ecosystem functioning. Thus, it is essential to conduct studies with model organisms to determine the impacts of these fungicides on different groups of living organisms. The aim of this study was to examine the ecotoxicity associated with exposure to commercial fungicides containing pyraclostrobin. The focus of the analysis involved germination and initial development of seedlings of 4 plant models (<i>Lactuca sativa</i>, <i>Raphanus sativus, Pennisetum glaucum</i> and <i>Triticum aestivum</i>), in addition to determining the population growth rate and total carbohydrate content in microalga <i>Raphidocelis subcapitata</i>. The fungicide pyraclostrobin adversely influenced growth and development of the tested plants, indicating a toxic effect. The fungicide exerted a significant impact on the initial development of seedlings of all model species examined with <i>T. aestivum</i> plants displaying the greatest susceptibility to pyraclostrobin. Plants of this species exhibited inhibitory effects on both aerial parts and roots when treated with a concentration of 4.75 mg/L pyraclostrobin. In addition, the green microalga <i>R. subcapitata</i> was also significantly affected by the fungicide, especially at relatively high concentrations as evidenced by a reduction in total carbohydrate content. This commercial fungicide demonstrated potential phytotoxicity for the tested plant models and was also considered toxic to the selected microalgae, indicating an ecotoxic effect that might affect other organisms in aquatic environments.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"973-987"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142301235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-cytogenotoxic potential assessment of two medicinal plants: <i>Davilla nitida</i> (Vahl) Kubitzki and <i>Davilla elliptica</i> (A. St.-Hill) (Dilleniaceae).","authors":"Fábio Eduardo Dos Santos, Daniel Rinaldo, Larissa Fonseca Andrade Vieira","doi":"10.1080/15287394.2024.2397649","DOIUrl":"10.1080/15287394.2024.2397649","url":null,"abstract":"<p><p>Humans have been using plants in the treatment of various diseases for millennia. Currently, even with allopathic medicines available, numerous populations globally still use plants for therapeutic purposes. Although plants constitute a safer alternative compared to synthetic agents, it is well established that medicinal plants might also exert adverse effects. Thus, the present investigation aimed to assess the phytotoxic, cytotoxic, and genotoxic potential of two plants from the Brazilian Cerrado used in popular medicine, <i>Davilla nitida</i> (Vahl) Kubitzki, and <i>Davilla elliptica</i> (A. St.-Hil.). To this end, germination, growth, and cell cycle analyses were conducted using the plant model <i>Lactuca sativa</i>. Seeds and roots were treated with 0.0625 to 1 g/L for 48 hr under controlled conditions. The germination test demonstrated significant phytotoxic effects for both species at the highest concentrations tested, while none of the extracts produced significant effects in the lettuce growth test. In the microscopic analyses, the aneugenic and cytotoxic action of <i>D. elliptica</i> was evident. In the case of <i>D. nitida</i> greater clastogenic action and induction of micronuclei, (MN) were noted suggesting that the damage initiated by exposure to these extracts was not repaired or led to apoptosis. These findings indicated that the observed plant damage was transmitted to the next generation of cells by way of MN. These differences in the action of the two species may not be attributed to qualitative variations in the composition of the extracts as both are similar, but to quantitative differences associated with synergistic and antagonistic interactions between the compounds present in these extracts.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"895-909"},"PeriodicalIF":2.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142121203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposome preparation of alpha-arbutin: stability and toxicity assessment using mouse B16F10 melanoma cells.","authors":"Altevir R Viana, Thatyana C Poleze, Franciele da S Bruckmann, Nathieli B Bottari, Luis R Peroza, Ingrid Rosales, Natalia S Zago, Maria R C Schetinger, Luciana M F Krause, Cristiano R B Rhoden, Sergio R Mortari","doi":"10.1080/15287394.2024.2393308","DOIUrl":"10.1080/15287394.2024.2393308","url":null,"abstract":"<p><p>Melanoma is the most aggressive type of skin cancer, with few therapeutic alternatives following metastasis development. In recent years, drug delivery-associated nanotechnology has shown promising targeted results with diminished adverse effects compared to conventional treatments. This study aimed to (1) examine the effects of plant-derived α-arbutin, a natural compound and (2) compare these findings with bioactively developed liposomes containing α-arbutin utilizing the B16-F10 murine melanoma cell line as a model. Liposomes were obtained through reversed-phase evaporation by applying a spray dryer to assess their stability. The following biologic assays were measured cytotoxicity/antiproliferative (MTT, Neutral Red, and dsDNA PicoGreen). In addition, the levels of melanin and purinergic enzymes were also measured. The production of reactive oxygen species (ROS) and nitric oxide (NO) was determined as a measure of oxidative state. Treatment with nano-liposome containing alpha-arbutin induced a significant 68.4% cytotoxicity, similar to the positive control, in the B16-F10 murine melanoma cell line at 72 hr. Further, arbutin and liposomes containing alpha-arbutin increased levels of ROS and nitrite formation at 72 hr at the highest concentration (100 and 300 µg/ml) of treatments. Arbutin and liposomes containing alpha-arbutin reduced melanin levels at all tested concentrations. In addition, arbutin and alpha-arbutin containing liposomes lowered nucleotides (AMP, ADP, and ATP) and nucleoside (adenosine) levels in melanoma cells. Evidence suggests that α-arbutin containing liposome can be considered as an alternative immunosuppressive agent stimulated in melanoma treatment.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"879-894"},"PeriodicalIF":2.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impacts of reduction in ambient fine particulate air pollution on natural-cause mortality in Taiwan.","authors":"Shang-Shyue Tsai, Chun-Yuh Yang","doi":"10.1080/15287394.2024.2384396","DOIUrl":"10.1080/15287394.2024.2384396","url":null,"abstract":"<p><p>Many epidemiologic studies have reported an association between high concentrations of fine particulate matter (PM_2.5) and increased mortality rates. Concurrently an association between decreased concentration of these airborne PM_2.5 pollutants and a decline in mortality frequency was noted in certain investigations globally; however, only a very few of these studies were conducted in Asia. Taiwan was found to exhibit a 30% decline in ambient PM_2.5 levels over the last 20 years. The aim of this ecological investigation was to examine the contribution of annual reductions in ambient PM_2.5 to changes in age-standardized natural-cause mortality rates (ASRs) in 65 townships in Taiwan from 2006 to 2020 controlling for lung cancer mortality rate, physician density, and annual household income. Data demonstrated a 0.9/10⁵ fall in adjusted ASR for every 10 ug/m³ reduction in mean annual PM_2.5 level in Taiwan during this 14-year period, suggesting a significant association between reductions in ambient PM_2.5 levels and decreases in natural-cause mortality rates.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"855-862"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141794138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnic-racial disparities in poisoning cases: analysis of drugs of abuse, medicines and pesticides in Brazil.","authors":"Niely Galeão da Rosa Moraes, Paula Florencio Ramires, Luíza Silva da Cruz, Júlia Oliveira Penteado, Romina Buffarini, Flavio Manoel Rodrigues da Silva Júnior","doi":"10.1080/15287394.2024.2389413","DOIUrl":"10.1080/15287394.2024.2389413","url":null,"abstract":"<p><p>In Brazil, ethnic-racial inequalities exist in all fields, obstructing access to goods, services, and opportunities, including healthcare services. However, there are no apparent studies that assess, at a national level, ethnic-racial disparities in poisoning cases, emphasizing skin color as a determining factor. The study aimed to examine the relationship between race/ethnicity and general poisoning cases, by medications, pesticides, and drug of abuse in Brazilian states. Poisoning cases data were extracted for the years 2017, 2018, and 2019. Notification data for general poisoning cases and toxic agents were collected: medications, pesticides, and drugs of abuse. Data were categorized between whites and non-whites (blacks, browns, and indigenous) and without information on skin color/ethnicity. Rates of poisonings amongst ethnic-racial groups and cases of not declared skin color as well as relative risk (RR) of poisoning among non-whites were calculated. All states in the North, Northeast (states with the worst Human Development Index), Midwest, and 2 states in the Southeast exhibited higher rates of poisoning cases per 100,000 inhabitants among non-whites. The RR values for nonwhite individuals were higher in the North and Northeast regions for all types of poisonings. The type of poisoning cases that presented the highest RR for non-whites over the 3 years was drugs of abuse (2-2.44), when compared to other types of poisonings from pesticides (2-2.33) and medications (1.5-1.91). The spatial distribution of poisoning cases rates and RR of nonwhite population support public policies to reduce socioeconomic and environmental inequalities.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"863-878"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid spectrophotometric test for assessing skin sensitization potential of chemicals by using <i>N</i>-acetyl-L-cysteine methyl ester <i>in chemico</i>.","authors":"Rahul Upadhyay Nepal, Tae Cheon Jeong","doi":"10.1080/15287394.2024.2372815","DOIUrl":"10.1080/15287394.2024.2372815","url":null,"abstract":"<p><p>During the key event 1 of skin sensitization defined as covalent binding or haptenization of sensitizer to either thiol or amino group of skin proteins, a sensitizer not only covalently binds with skin proteins but also interacts with nucleophilic small molecules such as glutathione (GSH). Although GSH would not be directly associated with skin sensitization, this interaction may be applied for developing an alternative test method simulating key event 1, haptenization. Thus, the aim of the present study was to examine whether <i>N</i>-acetyl-L-cysteine methyl ester (NACME), a thiol-containing compound, was selected as an electron donor to determine whether NACME reacted with sensitizers. Following a reaction of NACME with a sensitizer in a 96-well plate, the remaining NACME was measured spectrophotometrically using 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Following the optimization of test conditions with two different vehicles, such as acetonitrile (ACN) and dimethyl sulfoxide (DMSO), 64 test chemicals were tested to determine the predictive capacity of current NACME test method. The results obtained showed, the predictive capacity of 94.6% sensitivity, 88.9% specificity, and 92.2% accuracy utilizing DMSO as a vehicle with a cutoff NACME depletion of 5.85%. The three parameters were also over 85% in case of ACN. These values were comparable to or better than other OECD-approved test methods. Data demonstrated that a simple thiol-containing compound NACME might constitute as a reliable candidate for identifying reactive skin sensitizers, and that this method be considered as practical method as a screening tool for assessing a chemical's tendency to initiate skin sensitization.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"811-823"},"PeriodicalIF":2.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, cytotoxicity, antimicrobial, antibiofilm, and anti-quorum sensing potential of <i>Mentha Piperita</i> essential oil against the oral pathogen <i>Streptococcus mutans</i>.","authors":"Leonardo Quintana Soares Lopes, Pedro Henrique Fortes Guerim, Maria Eduarda Maldonado, Roger Wagner, Ana Carolina Hadlich Xavier, Jean Lucas Gutknecht da Silva, Daniella Bittencourt da Rosa Leal, Natália de Freitas Daudt, Roberto Christ Vianna Santos, Patrícia Kolling Marquezan","doi":"10.1080/15287394.2024.2375731","DOIUrl":"10.1080/15287394.2024.2375731","url":null,"abstract":"<p><p>Dental caries is a highly prevalent oral disease affecting billions of individuals globally. The disease occurs chemically as a result of breakdown of the tooth surface attributed to metabolic activity in colonizing biofilm. Biofilms, composed of exopolysaccharides and proteins, protect bacteria like <i>Streptococcus mutans</i>, which is notable for its role in tooth decay due to its acid-producing abilities. While various antimicrobial agents may prevent biofilm formation, these drugs often produce side effects including enamel erosion and taste disturbances. This study aimed to examine utilization of the <i>Mentha piperita</i> essential oil as a potential antibiofilm activity agent against <i>S. mutans</i>. <i>M. piperita</i> oil significantly (1) reduced bacterial biofilm, (2) exhibited a synergistic effect when combined with chlorhexidine, and (3) did not induce cell toxicity. Chemical analysis identified the essential oil with 99.99% certainty, revealing menthol and menthone as the primary components, constituting approximately 42% and 26%, respectively. Further, <i>M. piperita</i> oil eradicated preformed biofilms and inhibited biofilm formation at sub-inhibitory concentrations. <i>M. piperita</i> oil also interfered with bacterial quorum sensing communication and did not produce any apparent cell toxicity in immortalized human keratinocytes (HaCaT). <i>M. piperita</i> represented an alternative substance for combating <i>S. mutans</i> and biofilm formation and a potential combination option with chlorhexidine to minimize side effects. An <i>in-situ</i> performance assessment requires further studies.</p>","PeriodicalId":54758,"journal":{"name":"Journal of Toxicology and Environmental Health-Part A-Current Issues","volume":" ","pages":"824-835"},"PeriodicalIF":2.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}