New Microbiologica最新文献_第8页

Meta-Analysis of Demographic Disparities in Monkeypox Infections among Diverse Populations. 不同人群感染猴痘的人口统计学差异的 Meta 分析。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Mahmoud Kandeel

{"title":"Meta-Analysis of Demographic Disparities in Monkeypox Infections among Diverse Populations.","authors":"Mahmoud Kandeel","doi":"","DOIUrl":"","url":null,"abstract":"This meta-analysis aims to investigate demographic disparities in monkeypox (mpox) infections among various groups based on ethnicity, sexual partners, and gender. The study includes data from 2,646 to 4,002 patients across various outcomes. Among racial demographics, black populations show a lower odds ratio for mpox compared to white populations (OR=0.08 [0.01, 0.45], 95% CI, p=0.004). However, no statistically significant difference is found when comparing black populations with Hispanic or Asian populations (OR=0.72 [0.46, 1.11], p=0.13). There was a substantial disparity between gay, bisexual and other men-who-have-sex-with-men (GBMSM) and heterosexual populations, with significantly higher odds of mpox among the former (OR=393.80, 95% CI: [82.45, 180.85], p<0.00001). Analysis of sexual partners indicates a significant difference in infection risk between individuals with zero to one sexual partner and those with more than two partners (OR=0.06 [0.01, 0.28], p=0.0005). Additionally, there is a substantial difference in infection risk between male and female populations (OR=3868.02, p<0.00001). These findings emphasize the importance of considering demographic factors in understanding mpox transmission and risk profiles. Targeted research and intervention strategies are required to address the identified disparities and mitigate the spread of mpox.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"322-331"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Activity of Cefmetazole and Flomoxef among Extended-Spectrum Beta-Lactamase producing Enterobacterales. 头孢美唑和氟莫昔夫对产广谱β-内酰胺酶肠杆菌的体外活性

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Koji Iio, Hideharu Hagiya, Tsukasa Higashionna, Fumio Otsuka

{"title":"In vitro Activity of Cefmetazole and Flomoxef among Extended-Spectrum Beta-Lactamase producing Enterobacterales.","authors":"Koji Iio, Hideharu Hagiya, Tsukasa Higashionna, Fumio Otsuka","doi":"","DOIUrl":"","url":null,"abstract":"In this age of antimicrobial resistance (AMR), improving treatment using existing antibiotics is desirable. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales (ESBL-E) are high priority AMR pathogens according to the World Health Organization. Cephamycin-class beta- lactams are tolerant to hydrolysis by ESBL activity and have bactericidal effects on ESBL-E. The aim of the present study was to compare the in vitro minimum inhibitory concentration (MIC) of cefmetazole (CMZ) and flomoxef (FMOX) among ESBL-E strains. This was a retrospective study using microbiology laboratory data from Okayama University Hospital (Japan) from January 2014 to June 2022. The MIC was determined by broth microdilution method and the ESBL phenotypes were determined by double-disk method. Antimicrobial use density (AUD) data for CMZ and FMOX were also gathered. Annual proportions of ESBL-producing organisms in Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae complex were 20.4-30.6%, 3.5-13.7%, and 0-3.1%, respectively. The ESBL-producing bacteria with MIC levels ≤1 μg/mL for CMZ and FMOX ranged from 57 to 84% and 97 to 100%, respectively, for E. coli, and from 50 to 92% and 80 to 100%, respectively, for K. pneumoniae. E. cloacae strains showed MIC levels ≥32 μg/mL for both agents. The AUD ratio for CMZ to FMOX ranged from 5.31 to 12.27, with no apparent upward or downward trend. Proportions of ESBL-producing E. coli and K. pneumoniae strains with MIC ≤1 μg/mL were greater in FMOX than in CMZ. To corroborate the clinical superiority of FMOX in treating ESBL-E infections, a randomized controlled study, as well as pharmacokinetic/pharmacodynamic analysis, is required.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"348-353"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Mpox Virus in Seminal Fluids: Implications for Sexual Transmission. 检测精液中的 Mpox 病毒：对性传播的影响。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Angelo Roberto Raccagni, Antonella Castagna, Silvia Nozza

{"title":"Detection of Mpox Virus in Seminal Fluids: Implications for Sexual Transmission.","authors":"Angelo Roberto Raccagni, Antonella Castagna, Silvia Nozza","doi":"","DOIUrl":"","url":null,"abstract":"The 2022 outbreak of the human mpox virus, formerly known as monkeypox, raised global health concerns with widespread transmission across multiple countries. Sexual transmission emerged as a significant mode of spread, particularly among high-risk groups like MSM and PLWH. This manuscript focuses on the implications of seminal fluids in the transmission of mpox. The virus has been detected in various bodily fluids, including semen, indicating the potential for sexual transmission. Studies have reported high positivity rates of mpox DNA in seminal fluids. Despite some concern about possible contamination due to genital lesions, the presence of replication-competent virus in seminal fluids has been confirmed and mpox virus was also detected in this specimen among people who engaged only in receptive sexual intercourse. Antiviral treatment with tecovirimat showed efficacy in reducing viral presence in semen with detection of the antiviral in this specimen. Virus clearance from semen is relatively rapid and parallels healing from infection, with no reported cases of seminal fluid relapses. The WHO recommendation to avoid condomless intercourse for 12 weeks after clinical healing still appears prudent. Continued research and surveillance are essential to understand viral dynamics and develop effective prevention measures to combat the spread of mpox through sexual transmission and protect key-populations.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"317-321"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of canine parvovirus 2 (CPV-2) replication by TAT-scFv through targeting of the viral structural protein VP2 of CPV-2. TAT-scFv 通过靶向 CPV-2 的病毒结构蛋白 VP2 抑制犬细小病毒 2（CPV-2）的复制。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Kun Liu, Peng Xu, Yuchen Li, Jiali Qin, Jiping Zhu, Yi Li

{"title":"Inhibition of canine parvovirus 2 (CPV-2) replication by TAT-scFv through targeting of the viral structural protein VP2 of CPV-2.","authors":"Kun Liu, Peng Xu, Yuchen Li, Jiali Qin, Jiping Zhu, Yi Li","doi":"","DOIUrl":"","url":null,"abstract":"Canine parvovirus (CPV) causes severe infectious disease with a high mortality rate in dogs. CPV is still a major health issue of dogs in the clinic. Therefore, there is an urgent need to develop effective drugs to treat the disease. In this study, we fused the transactivating transcriptional activator peptide (TAT) with scFv. TAT-scFv was identified by Western blot. CCK8 kit was used to detect the toxicity of TAT-scFv to cells. The binding activity of TAT-scFv to CPV-2-VP2 was detected by DAS ELISA. The cell uptake rate of TAT-scFv was assessed by IFA. After infection with CPV-2, F81 cells were incubated by TAT-scFv. The replication of virus was measured to determine the neutralization effect of TAT-scFv on intracellular and extracellular viruses. Protein docking was used to predict the amino acid (AA) sites of VP2 binding to TAT-scFv. TAT-scFv was expressed in Escherichia coli and purified. The DAS ELISA showed that TAT-scFv could bind with CPV-2-VP2. We demonstrated that TAT-scFv entered cells in a dose-dependent and time-dependent manner and effectively inhibited the replication of CPV-2. Using protein docking, we determined the interaction pattern and found that the N-terminal region (AA 41-49) and the C-terminal region (AA 558) of VP2 interacted with the TAT-scFv. Taken together, these results suggest that, TAT-scFv may be a potential antiviral drug for inhibiting CPV-2 replication and controlling disease caused by CPV-2.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"381-389"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of inhibitory active substances produced by Pseudoalteromonas SW-1. 假交替单胞菌 SW-1 产生的抑制性活性物质的特征。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Xing Sun, Xin Xu, Xuhui Wang, Tongtong Tang, Weiwei Zhang, Yuhua Ma

引用次数: 0

First case of Chryseobacterium gallinarum bloodstream infection: a diagnostic and therapeutic challenge for an emerging pathogen. 首例胆囊分枝杆菌血流感染病例：新出现病原体在诊断和治疗方面的挑战。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Mattia Genco, Antonio Curtoni, Paolo Bottino, Silvia Scabini, Silvia Corcione, Davide Vita, Luisa Guarrasi, Gabriele Bianco, Alessandro Bondi, Daniela Risso, Rossana Cavallo, Cristina Costa

引用次数: 0

Successful control measures to treat the transmission of Candida auris in Northern Italian Hospital. 成功控制意大利北部医院的念珠菌传播。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Federico Giovagnorio, Nicholas Geremia, Claudio Scarparo, Sandro Panese, Simone Bradariolo, Chiara Berti, Maria Solinas, Maurizio Sanguinetti, Vittorio Selle, Andrea Cozza, Saverio Giuseppe Parisi, Giovanni Carretta

引用次数: 0

18S rRNA sequencing and homology analysis of post-traumatic bloodstream infection with Saprochaete clavata (Magnusiomyces clavatus) and a case report. 创伤后血流感染克拉瓦塔无患子（克拉瓦塔木霉菌）的 18S rRNA 测序和同源性分析及病例报告。

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Wei Jia, Guang Chen, Wanxiang Li, Jie Ma, Xue Zhao

引用次数: 0

Neonatal severe COVID-19 infection complicated by Staphilococcus aureus could be misinterpreted as MIS-C?: case report and review of literature. 新生儿严重 COVID-19 感染并发金黄色葡萄球菌可能被误诊为 MIS-C？

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Giovanna Stringari, Laura Nai Fovino, Aldo Naselli, Francesca Tota, Michela Capogna, Silvia Graziani, Massimo Soffiati

{"title":"Neonatal severe COVID-19 infection complicated by Staphilococcus aureus could be misinterpreted as MIS-C?: case report and review of literature.","authors":"Giovanna Stringari, Laura Nai Fovino, Aldo Naselli, Francesca Tota, Michela Capogna, Silvia Graziani, Massimo Soffiati","doi":"","DOIUrl":"","url":null,"abstract":"At 23 days of life a neonate presented to the emergency room with crying and decreased oral intake. His parents were positive to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), but he turned out negative. After one week he was admitted to NICU (neonatal intensive care unit) for respiratory failure, and nasopharyngeal swab (PCR test: polymerase chain reaction test) was positive for SARS-CoV-2. On examination the child had fever, tachy-dyspnea, reduced oxygen saturation, tachycardia, abdominal distension and tenderness, irritability and hypertonia. Blood exam showed respiratory acidosis, lymphocytopenia, hypoalbuminemia and coagulopathy; CRP (C reactive protein), procalcitonin, D-dimer, ferritin and NT-proBNP (N-terminal prohormone of brain natriuretic peptide) were elevated. Chest X-ray revealed bilateral interstitial infi ltration and abdomen ultrasound a thin fl uid effusion; echocardiography was normal. SARS-CoV-2 PCR tests on CSF (cerebrospinal fluid) and stool were also positive. He was started on non-invasive intermittent positive pressure respiratory ventilation, treated with antibiotic therapy, methylprednisolone, intravenous immunoglobulins, and antiplatelet therapy. Rapid clinical improvement was seen with remission of fever after eight days. The child complicated with bacterial super-infection presenting as pleural empyema. As presented in our case, it is not always easy to differentiate between severe forms of COVID-19 and MIS-C. Due to the rarity of these presentations in neonates, multicentric collaboration is needed to identify the specifi c characteristics of the two forms, better defi ne diagnostic criteria, and treatment options.","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"390-394"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Medicinal Plant Extracts and Antimicrobial Magistrals. 药用植物提取物和抗菌药的比较评估

IF 1.8 4区医学

New Microbiologica Pub Date : 2024-01-01

Hager Muftah, Berrin Özçelik, Ozlem Oyardi, Didem Deliorman Orhan

{"title":"Comparative Evaluation of Medicinal Plant Extracts and Antimicrobial Magistrals.","authors":"Hager Muftah, Berrin Özçelik, Ozlem Oyardi, Didem Deliorman Orhan","doi":"","DOIUrl":"","url":null,"abstract":"The in vitro antimicrobial activity of the active ingredient in antimicrobial magistral drug formulations and plant extracts used in folk medicine were investigated comparatively. Borax, sulfur colloid, hydrogen peroxide, benzoic acid, rivanol, brilliant green and plant extracts as active ingredients, namely: Helianthus tuberosus tuber-H2O (aqueous extract), Cydonia oblonga leaves-H2O, Allium porrum whole plant-H2O, Cistus laurifolius leaves-EtOH, Solanum muricalum-H2O, and Fumaria cilicica leaves-EtOH were studied to determine their antimicrobial activity against different bacteria and fungi (S. pyogenes, S. aureus, S. epidermidis, E. faecalis, K. pneumonia, H. influenza, P. aeruginosa, A. baumannii, E. coli, Candida albicans, C. tropicalis, C. parapsilosis, C. krusei) by using the microdilution method. The active ingredients and plant extracts showed different activities as MIC between 1->128 μg/mL. Brilliant green and rivanol as active ingredients had MIC values of 1 μg/mL against all tested microorganisms. C. oblonga leaves-H2O as well as C. laurifolius leaves-EtOH as plant extracts were indicated as having the highest antimicrobial effect in MIC value of 16 μg/ml against A. baumannii and S. pyogenes, respectively. On the other hand, F. cilicica leaves-EtOH and C. laurifolius leaves-EtOH showed the highest antifungal activity (MIC; 16 μg/mL).","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"46 4","pages":"361-366"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139514248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0