Onderstepoort Journal of Veterinary Research最新文献

{"title":"A potential antifungal bioproduct for Microsporum canis: Bee venom.","authors":"Armağan E Ütük, Tülin Güven Gökmen, Hatice Yazgan, Funda Eşki, Nevin Turut, Şifa Karahan, İbrahim Kıvrak, Sedat Sevin, Osman Sezer","doi":"10.4102/ojvr.v91i1.2191","DOIUrl":"https://doi.org/10.4102/ojvr.v91i1.2191","url":null,"abstract":"<p><p>Natural treatment options for Microsporum canis dermatophytosis are being explored because of resistance to several antifungal medications. In this study, the potential antifungal effect of bee venom (BV), a natural antimicrobial agent, on M. canis was investigated. The antifungal effects of BV, fluconazole, itraconazole, amphotericin B and terbinafine were evaluated by the macrodilution method at various concentrations by modifying the microdilution method recommended by the European Committee on Antimicrobial Susceptibility Testing. All isolates were observed to be susceptible to terbinafine and fully resistant to fluconazole and amphotericin B. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of M. canis isolate 2 (Mc2) were determined as 8 µg/mL for itraconazole. The MIC and MFC values of BV were found to be 320 µg/mL for the Mc2 isolate and 640 µg/mL for the Mc6 isolate. The results showed that the isolates obtained from clinical samples in this study were highly resistant to all antifungal agents, except terbinafine. The increase in resistance indicates that antifungal drugs will become insufficient and ineffective over time and natural products such as BV should be evaluated as alternatives.Contribution: Although there are many drugs for the treatment of M. canis, the increase in resistance to antifungal agents reveals the need for the identification and development of new natural agents. Bee venom, which has been shown to have a safe and weak allergenic effect in various studies, can be tested for usability as a local antifungal drug when supported by in vivo studies.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e6"},"PeriodicalIF":1.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method validation and antioxidant activities of Hyperacanthus amoenus and Carissa bispinosa.","authors":"Kedibone G Kgosana, Tirelo Matlala","doi":"10.4102/ojvr.v91i1.2182","DOIUrl":"https://doi.org/10.4102/ojvr.v91i1.2182","url":null,"abstract":"<p><p>Plant foliages used as feed additives pose a health risk due to high oxidant concentrations. Oxidants cause oxidative stress and high rate of morbidities and mortalities. Hence, the aim of the study was to validate the methods to quantify gallic acid (GA) and quercetin (Q) as putative antioxidants, and to evaluate antioxidant activities in feed (F), Hyperacanthus amoenus (HA) and Carissa bispinosa (CB) extracts. Extraction was carried out with 62.5% methanol. Method validations for linearity, accuracy and precision were performed on high performance liquid chromatography. Quantitative analysis of GA and Q and testing of 2,2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities in the extracts were performed. The lowest limit of detection (LOD) and limit of quantitation (LOQ) of 0.011 µg/mL and 0.032 µg/mL were determined in HA, respectively. The methods were accurate and precise as the relative standard deviations (%RSD) were less than 15%. The GA concentrations in CB and HA extracts were statistically significant (p  0.05) and their values were 0.65 ± 0.03 x 106 µg/kg dry weight (DW) (0.13%) and 0.28 ± 0.06 x 106 µg/kg DW (0.002%), respectively. All extracts showed very strong radical scavenging activities with their IC50 values ranging between 5.87 µg/mL and 6.86 µg/mL.Contribution: These accurate, repeatable, precise and reliable methods can be used to provide a valuable basis for GA and Q analysis in various shrub foliages. Though high GA concentrations have potential to act as antioxidants, they may have adverse health and growth performance effects when used as feed additives, while lower Q concentrations may have no effects on livestock.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e10"},"PeriodicalIF":1.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coinfection of some respiratory viruses in cattle: An abattoir study.","authors":"Intisar K Saeed, Yahia H Ali, Muaz Magzoub Abdellatif, Alaa Mustafa, Ahmad M Abdel-Mageed","doi":"10.4102/ojvr.v91i1.2193","DOIUrl":"https://doi.org/10.4102/ojvr.v91i1.2193","url":null,"abstract":"<p><p>To investigate the existence of bovine viral diarrhoea virus (BVDV), parainfluenza virus 3 (PIV-3) and respiratory syncytial virus (RSV) as well as its coinfections, a total of 420 pneumonic lung tissue samples were collected from slaughterhouses in three different areas. Samples were examined for the three viruses using antigen detection enzyme-linked immunosorbent assay (ELISA) test, and positive results were further confirmed using fluorescent antibody test and polymerase chain reaction. Prevalences detected were 10.47% for BVDV, 11.9% for parainfluenza virus type (PIV-3) and 7.6% for respiratory syncytial virus (RSV). Observed coinfections were bovine viral diarrhoea (BVD) and PIV-3 in 8.1%, BVD and RSV in only two samples (1%). Existence of all three viruses was detected in two samples (1%).Contribution: In this study, infection of the three viruses was confirmed in cattle and existence of its coinfection is documented for the first time.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e8"},"PeriodicalIF":1.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taenia saginata prevalence in cattle slaughtered at low throughput abattoirs in South Africa.","authors":"Mbali P Dube, Charles Byaruhanga, Pierre Dorny, Veronique Dermauw, Daniel N Qekwana","doi":"10.4102/ojvr.v91i1.2157","DOIUrl":"https://doi.org/10.4102/ojvr.v91i1.2157","url":null,"abstract":"<p><p>Meat inspection is the routine method used to identify cattle infected with Taenia saginata; however, the sensitivity of this method is low. We investigated the prevalence of T. saginata infection in cattle slaughtered in low throughput abattoirs (LTs) in Gauteng province, South Africa, based on meat inspection and serology. A total of 188 cattle carcasses from three abattoirs underwent meat inspection for the presence of T. saginata cysticerci, while serum was tested for the occurrence of antigens using antigen enzyme-linked immunosorbent assay (Ag-ELISA). Polymerase chain reaction (PCR)-sequencing was performed to confirm T. saginata cysts identified during enhanced inspection and incision of 10 randomly selected hearts. Apparent and true prevalence were calculated, and a logistic regression model was fit to evaluate associations between abattoir, sex, animal origin, age and T. saginata serological status. Out of the 188 carcasses, no positive cases (0%) were identified during routine meat inspection, yet three cysticerci-like lesions were identified from three hearts following additional incisions, of which one was confirmed as T. saginata. Fifty-four of the sampled cattle carcasses tested positive using Ag-ELISA (apparent and true prevalence: 29.0%, 71.8%). Feedlot cattle were less likely (odds ratios [OR]: 0.33, p = 0.043) to have a positive serological test result for T. saginata compared to non-feedlot cattle, and the odds of a positive result differed between abattoirs (p  0.05).Contribution: Our results confirmed the low sensitivity of routine meat inspection in LTs, which may pose a public health risk, and therefore other diagnostic methods need to be included in the surveillance system for T. saginata.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e8"},"PeriodicalIF":1.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Managing zoonotic infectious diseases in Africa: The key role approach.","authors":"Abdalla A Latif","doi":"10.4102/ojvr.v91i2.2195","DOIUrl":"10.4102/ojvr.v91i2.2195","url":null,"abstract":"<p><p>No abstract available.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 2","pages":"e1-e2"},"PeriodicalIF":1.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11622108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant properties, anti-nutritive and toxic factors of Terminalia sericea in Onderstepoort.","authors":"Tirelo Matlala, Rejoice B Maseko, Kedibone G Kgosana","doi":"10.4102/ojvr.v91i1.2172","DOIUrl":"10.4102/ojvr.v91i1.2172","url":null,"abstract":"<p><p>Anti-nutritive and toxic factors in plants adversely affect animal health and production. Hence, the aims of the study were to (1) carry out qualitative and quantitative analysis of these factors in Terminalia sericea that grows abundantly in Onderstepoort; (2) evaluate an affordable, efficient and reliable method that reduces these factors and (3) evaluate antioxidant properties of the phenolic compounds. Thus, sequential organic and aqueous extractions were carried out on T. sericea leaves and feed pellets. Extracts were subjected to qualitative analysis to confirm the presence of 11 phytochemicals. While quantitative analysis of nine phytochemicals was carried out on spectrophotometry, the phenolic extracts were further tested for antioxidant activity on 2,2-diphenyl-picrylhydrazyl (DPPH) assay where gallic acid (GA), quercetin (Q) and ascorbic acid (AA) were used as standards. The contents of GA, Q and AA in the phenolic extracts were determined on high-performance liquid chromatography (HPLC). Aqueous extraction was an effective method as most phytochemicals were extracted. Nine phytochemicals were qualitatively analysed in T. sericea. The highest contents of 1.97% and 2.37 ± 0.077 mg/g gallic acid equivalent with a significant difference (p  0.05) were determined in oxalates and phenolics, respectively. Furthermore, 83.1% DPPH inhibition (IC50 = 0.0432 µg/mL) was determined relative to the standards (85.27% - 85.96%; IC50 = 0.000009 µg/mL - 0.00042 µg/mL). Subsequently, HPLC results showed that T. sericea contained high concentration of AA (62.5 ± 2.13 µg/mL) to confirm antioxidant properties of the leaves.Contribution: Though T. sericea leaves have antioxidant activities, the alkaloid contents may pose a threat to the livestock. Therefore, farmers around Onderstepoort should protect their livestock from feeding on these leaves.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e15"},"PeriodicalIF":1.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance and mitigation using One Health lens in aquaculture of Northern Nigeria.","authors":"Nma B Alhaji, Ismail Ayoade Odetokun, Mohammed S Jibrin, Mohammed K Lawan, Jacob Kwaga","doi":"10.4102/ojvr.v91i2.2165","DOIUrl":"10.4102/ojvr.v91i2.2165","url":null,"abstract":"<p><p>A cross-sectional study was conducted to assess antibiotic usage, residues, resistance and drivers of their emergence in fish aquaculture in Northern Nigeria between 2019 and 2020. A structured questionnaire was administered to 151 randomly selected farmers. Fish, column and wastewater samples were analysed with enzyme link immunosorbent assay (ELISA) for residue detection and quantification. We performed descriptive and analytic statistical analyses. All selected farmers participated, 78.1% used antibiotics in ponds. Majority (77.1%) of the farmers did not know what antibiotic misuse entailed and 73.3% mentioned that antibiotic misuse and overuse cannot predispose to residues and resistance. The most frequently used antibiotics were tetracyclines (TCs) (99.2%). Significant risk routes for residue and resistance spread were: contaminated fish and its products ingestion, direct contact with fish and fomites contaminated as well as water and aerosols contaminated in the pond environment. Significant social and cultural that influenced residue and resistance development include antibiotics misuse/overuse (Odds Ratio [OR] = 3.8; 95% confidence interval [CI]: 1.62-8.74) as well as poor education and expertise of the farmers (OR = 2.9; 95% CI: 1.24-6.94). The mean TCs residues level in column and wastewater was 123.6 ± 18.2 μg/kg-1. Poor knowledge and attitudes regarding antibiotic usage were identified. Adequate antibiotic stewardship should be promoted through farmers' education. Identified social and cultural factors can be mitigated through the 'One Health' approach.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 2","pages":"e1-e11"},"PeriodicalIF":1.5,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rabies in equids in Sudan.","authors":"Yahia H Ali, Tenzeil A G Mohieddeen, Muaz M Abdellatif, Baraa Mohammed Ahmed, Intisar K Saeed, Husham M Attaalfadeel, Amani A Ali","doi":"10.4102/ojvr.v91i1.2181","DOIUrl":"10.4102/ojvr.v91i1.2181","url":null,"abstract":"<p><p>Rabies is endemic in Sudan with continuing outbreaks occurring annually, the most common animals affected are dogs, followed by goats and equids. This work focused on equid rabies, to elucidate the current situation of the disease through analysis of reports of equid rabies outbreaks in Sudan during 2010-2022 supported by laboratory confirmation of the disease. During the study period, 66 animals were affected during 35 equid rabies outbreaks. The highest incidences were found in Al Gezira (30.3%), followed by Darfur (24.2%) and Kordofan (15.2%). The highest incidence rate was observed during 2018 (33.3%), followed by 2015 (16.7%). Within seasons, the highest incidence rate was reported during October - December (33.3%), followed by July - September (30.3%). Chi-square analysis revealed a significant correlation between rabid animals and year, season, and state. Wald statistics demonstrated that year and season had a significant association with the disease. Virus antigen was identified (72.2%) in brain tissues using the fluorescent antibody test. Viral nucleic acid was amplified (n = 6) with a reverse transcriptase polymerase chain reaction assay.Contribution: As equids are kept in close contact with humans and other animals in the country, according to the present investigation, equid rabies in Sudan is a potential public health concern, emphasising the importance of implementing effective control measures.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 1","pages":"e1-e10"},"PeriodicalIF":1.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applied One Health: Nigeria National Veterinary Research Institute COVID-19 pandemic response.","authors":"Clement A Meseko, Ismaila Shittu, Olayinka O Asala, Adeyinka J Adedeji, Tinuke A Laleye, Ebere R Agusi, Dorcas A Gado, Kayode A Olawuyi, Nicodemus Mkpuma, Chinyere Chinonyerem, Bitrus Inuwa, Nneka Chima, Ruth Akintola, Patrick Nyango, Hellen Luka, Judith Bakam, Rebecca Atai, Dennis Kabantiyok, Mark Samson, ThankGod Daniel, Joshua Oyetunde, Olajide A Owolodun, David D Lazarus, Emmanuel T Obishakin, Pam D Luka, Benshak J Audu, Sunday Makama, Hussaini G Ularamu, Yiltawe S Wungak, James S Ahmed, Reuben A Ocholi, Maryam Muhammad","doi":"10.4102/ojvr.v91i2.2156","DOIUrl":"10.4102/ojvr.v91i2.2156","url":null,"abstract":"<p><p>The COVID-19 pandemic has caused the death of 7.1 million people worldwide as of 7 July 2024. In Nigeria, the first confirmed case was reported on 27 February 2020, subsequently followed by a nationwide spread of SARS-CoV-2 with morbidity and mortality reaching 267 173 and 3155, respectively, as of 7 July 2024. At the beginning of the pandemic, only a few public health laboratories in Nigeria had the capacity for SARS-CoV-2 molecular diagnosis. The National Veterinary Research Institute (NVRI), already experienced in influenza diagnosis, responded to the public health challenge for the diagnosis of COVID-19 samples from humans. The feat was possible through the collective utilisation of NVRI human and material resources, including biosafety facilities, equipment, reagents and consumables donated by international partners and collaborators. Within 6 months of the reported COVID-19 outbreak in Nigeria, over 33 000 samples were processed in NVRI facilities covering five states. Thereafter, many field and laboratory projects were jointly implemented between NVRI and collaborating sectors including the Nigerian Centre for Disease Control (NCDC) and the National Institute for Medical Research (NIMR), which brought together professionals in the health, veterinary, education and socio-sciences. In addition, One Health grants were secured to enhance surveillance for coronavirus and other zoonoses and build capacity in genomics. Bio-surveillance for coronaviruses and other emerging zoonotic pathogens at the human-animal interface was activated and continued with sample collection and analysis in the laboratory for coronaviruses, Lassa fever virus and Mpox. One Health approach has shown that inter-sectoral and multinational collaboration for diagnosis, research and development in animals, and the environment to better understand pathogen spillover events at the human-animal interface is an important global health priority and pandemic preparedness.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 2","pages":"e1-e6"},"PeriodicalIF":1.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with the rabies vaccination status of dogs in households in Beni City, D.R. Congo.","authors":"Prince D Kimpanga, Emery K Taghembwa, Gauthier M Mubenga, Jean-Marie T Makwera, Norbert M Muhongya, Odrade B Chabikuli, Justin M Masumu","doi":"10.4102/ojvr.v91i2.2168","DOIUrl":"10.4102/ojvr.v91i2.2168","url":null,"abstract":"<p><p>Human rabies transmitted by dogs still kills thousands of people each year worldwide. Dog bites are common in the city of Beni (Democratic Republic of Congo), which shows low rabies vaccination coverage. This study aimed to determine the factors associated with the rabies vaccination status of dogs. A cross-sectional analytical study was conducted in the town of Beni among dog owners, during a household survey selected using a multistage sampling. The information sought concerned the knowledge and characteristics of the dog owners as well as the vaccination status of these dogs. Logistic regression was used to investigate associations between the vaccination status of the dogs and the main independent factors. Rabies vaccination coverage in Beni was 26% (95% confidence interval [CI]: 22% - 30%). The main factors associated with the rabies vaccination status of the dog were primary education level of household head (adjusted odds ratio [aOR]:4.8; 95% CI: 1.2- 19.8); university education level of household head (aOR: 5.9; 95% CI: 1.6-22); perceived rabies severity (aOR: 44. 4; 95% CI: 10.4-188), having more than one dog in the household (aOR: 2.6; 95% CI: 1.6-4.3); age range 7-12 months (aOR: 0.2; 95% CI: 0.1-0.6) and confined dog breeding (aOR: 3.9; 95% CI: 1.1-14.9). The low vaccination coverage in Beni requires mass vaccination campaigns against canine rabies targeting the dog owners with low education levels, those raising more than one dog, with stray dogs or dogs less than 12 months old.</p>","PeriodicalId":54685,"journal":{"name":"Onderstepoort Journal of Veterinary Research","volume":"91 2","pages":"e1-e6"},"PeriodicalIF":1.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11369842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}