Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology?

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Motlatso T Hlokwe, Nomawethu S Masina, Boitumelo Letsoko, Sewellyn C Davey, Anita L Michel
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Abstract

Paratuberculosis (Johne's disease) has devastating outcomes on ruminant health and impacts on national and international trade. The current work assessed the diagnostic value of the VersaTREK automated liquid culture system in isolating Mycobacterium avium subspecies paratuberculosis (MAP) from faecal and intestinal tissue samples from ovine under South African conditions and compared it with the current method of choice, histopathological examination. Intestinal tissue and faecal samples from 111 sheep (including complete set from 104 slaughter sheep from flocks with a history of MAP infection as well as incomplete sample sets from 7 sheep) were analysed using the liquid culture method. One set of tissues was subjected to histopathological examination. Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. Overall, the VersaTREK automated liquid culture in combination with IS900 PCR showed a comparable level of detection in tissues (12.6%) as histopathology (13.5%), but the detection rate for faecal samples was lower than for tissues (10.8%). A combination of histopathology and faecal culture increased the detection rate from 13.5% (n = 14/104) and 9.6% (n = 10/104), respectively, to 15.4% (n = 16/104).Contribution: Our findings highlight the diagnostic utility of the VersaTREK automated liquid culture system in detecting MAP in ovine samples collected both ante and postmortem. However, an inhibitory effect on the MAP isolation rate observed when the antibiotic cocktail was added to the culture medium warrants further investigation. The outcome of the study is beneficial in guiding the strategic planning of the nationwide control programme.

与组织病理学相比,基于培养的鸟分枝杆菌副结核检测有什么好处?
副结核病(约翰氏病)对反刍动物的健康造成毁灭性后果,并对国家和国际贸易产生影响。目前的工作评估了VersaTREK自动液体培养系统在南非条件下从绵羊粪便和肠道组织样本中分离鸟分枝杆菌亚种副结核(MAP)的诊断价值,并将其与当前选择的组织病理学检查方法进行了比较。采用液体培养法对111只羊的肠道组织和粪便样本(包括104只有MAP感染史的屠宰羊的完整样本和7只羊的不完整样本)进行分析。其中一组组织行组织病理学检查。从培养分离物中提取的脱氧核糖核酸(DNA),使用针对MAP IS900区域的引物进行聚合酶链反应(PCR)扩增,以进行物种验证。总体而言,VersaTREK自动液体培养结合IS900 PCR在组织中的检出率(12.6%)与组织病理学(13.5%)相当,但粪便样本的检出率低于组织(10.8%)。组织病理学和粪便培养相结合使检出率分别从13.5% (n = 14/104)和9.6% (n = 10/104)提高到15.4% (n = 16/104)。贡献:我们的研究结果强调了VersaTREK自动液体培养系统在检测羊死前和死后样本中MAP的诊断效用。然而,在培养基中加入抗生素鸡尾酒对MAP分离率的抑制作用值得进一步研究。研究结果对指导全国防治工程的战略规划具有指导意义。
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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
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