Plant Omics最新文献

{"title":"Identification of microRNA elements from genomic data of European hazelnut (Corylus avellana L.) and its close relatives","authors":"Bihter Avşar, Danial Esmaeilialiabadi","doi":"10.21475/POJ.10.04.17.PNE707","DOIUrl":"https://doi.org/10.21475/POJ.10.04.17.PNE707","url":null,"abstract":"Plant microRNAs (miRNAs) are small and non-coding endogenous RNAs which have numerous regulatory roles in cells. These critical players regulate pathways either by inducing translational repression or messenger RNA (mRNA) decay. Newly developed bioinformatics tools and computational methods have been increased to identify miRNAs with their targets inside the genome. In this study, we predicted and identified 57 putative miRNAs through Corylus avellana (C. avellana) genomic data in silico. We also predicted some other putative miRNAs from Arabidopsis thaliana (A. thaliana), Ricinus communis (R. communis), Populus trichocarpa (P. trichocarpa) and Vitis vinifera (V. vinifera) to compare with the C. avellana organism since previous studies have indicated high similarities between these genomes and proteome atlases. The miRBase 21 was used as a reference dataset and the putative miRNAs were identified for the genome of each organism. We used homology conserved method to identify putative miRNAs. Based on our findings, C. avellana miRNA content was found to be highly similar to V. vinifera, R. communis and P. trichocarpa. Also, we found the targets of these hazelnut putative miRNAs and their possible functions inside the cell. One of our major discoveries is that miR171 families are highly represented (the copy number of miRNA) in the hazelnut genome to provide clues for microRNA domestication. The miR396, miR482, and miR2118 families were found as in silico expressed putative miRNAs by using computational methods. All these findings may help us better understanding the miRNA repertoire of the hazelnut organism and provide valuable insight about the regulatory roles of predicted putative miRNAs which are shared with other organisms (A. thaliana, R. communis, P. trichocarpa, V. vinifera) for further studies.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"190-196"},"PeriodicalIF":0.0,"publicationDate":"2017-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45361008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis of long term salt responsive proteins in the halophyte Suaeda maritima","authors":"S. Krishnamurthi, Suja George, S. Meenakshisundram, A. Parida","doi":"10.21475/POJ.10.04.17.PNE734","DOIUrl":"https://doi.org/10.21475/POJ.10.04.17.PNE734","url":null,"abstract":"A proteomic approach was carried out to identify proteins responsive to long term salt treatment in the halophyte Suaeda maritima. A 3-month-old S. maritima seedlings were hydroponically exposed to Modified Hoagland’s treated with salt solution of 200mM were grown for a period of 14 days in a growth chamber maintained at 24 ± 3°C, 70-75% relative humidity with 14 h light (200 μmol m s)/10 h dark cycle. The untreated set maintained in Modified Hoagland’s solution was considered as control with similar conditions. Proteins extracted from the leaves of S. maritima control and salt treated seedlings were separated by Two-Dimensional gel electrophoresis (2DE). Using PDquest software analysis, we observed ~ 50 protein spots were reproducibly detected on gels, out of which 18 differentially expressed protein spots showed at least two-fold differences on 2DE maps some of them were up-regulated and few others were down-regulated in treated compared with the control. From that, we identified 6 up-regulated protein spots shows the maximum level of fold differences and these protein spots were performed trypsin digestion and Matrix Assisted Laser Desorption Ionization -Time of Flight (MALDI-TOF) analysis. The results showed that S. maritima could withstand up to 200mM NaCl for long term period of 14 days by up regulating proteins that are mainly involved in protein transport, vesicle trafficking, heme/iron binding, protein folding and assembly, chromosome segregation, cell maintenance. Our study has identified salt responsive proteins such as RAB2B, CYP71A8, SCC3 that are not previously identified by expressed sequence tag analyses or transcriptome analyses in this species. This is the first report of proteome analysis and provides insights into the molecular mechanisms of long term salt tolerance in S. maritima.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"197-204"},"PeriodicalIF":0.0,"publicationDate":"2017-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46194859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative tolerance of different species of Brassica to cadmium toxicity: Coordinated role of antioxidant defense and glyoxalase systems","authors":"J. Mahmud, M. Hasanuzzaman, K. Nahar, Anisur Rahman, M. Fujita","doi":"10.21475/POJ.10.02.17.PNE409","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE409","url":null,"abstract":"The present study was carried out to examine the metal accumulation and tolerance abilities of three 'Brassica' species ('B. napus, B. campestris, and B. juncea') seedlings exposed to two levels of cadmium (Cd) stress (0.25 and 0.5 mM CdCl2 for three days). Of the 'Brassica' species studied, 'B. juncea' accumulated the highest amount of Cd in a dose-dependent manner, and in every case, the Cd content was higher in the roots than the shoots. Cadmium stress reduced seedlings biomass, leaf relative water content (RWC), and chlorophyll (chl) content, whereas proline (Pro), MDA, and H2O2 content and lipoxygenase (LOX) activity increased in all species. Under Cd stress, ascorbate (AsA) content reduction was lower and glutathione (GSH) content increase was higher in 'B. juncea' compared with other species. Monodehydroascorbate reductase (MDHAR), glutathione reductase (GR), and superoxide dismutase (SOD) activities increased significantly in 'B. juncea' under Cd stress compared with the other species. Catalase (CAT) activity did not decrease in 'B. juncea' due to Cd stress, compared with the other species. Dehydroascorbate reductase (DHAR) activity decreased with both levels of Cd stress in all species except for 'B. juncea' under 0.25 mM CdCl2 stress. Glyoxalase system components performed better in 'B. juncea' than the other species under Cd stress. Methylglyoxal (MG) increased substantially under both levels of Cd stress, but MG content was lower in 'B. juncea' compared with the others. Considering the antioxidant defense and glyoxalase systems performance B. juncea is relatively tolerant species to Cd toxicity though it accumulated highest Cd.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"107-117"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46003666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico mining and characterization of simple sequence repeats (SSRs) from Euphorbia esula expressed sequence tags (ESTs): A potential crop for biofuel","authors":"Surojit Sen, B. Dehury, J. Sahu, Sunayana Rathi, R. Yadav","doi":"10.21475/POJ.10.02.17.PNE370","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE370","url":null,"abstract":"Euphorbia esula (leafy spurge) is a perennial noxious weed native to Eurasia, which can also be potentially useful as a biofuel, medicine, or a pest control agent. In this study, we analysed publicly available ESTs of ‘NCBI dbEST’ (http://www.ncbi.nlm.nih.gov/dbEST) using in silico tools to have an insight into the genetic makeup of the plant. Mining of simple sequence repeats (SSRs) was performed by MISA, primer designing by Primer3 while functional annotation, gene ontology and enrichment analysis were performed by Blast2GO. SSR mining from 47543 ESTs revealed a total 3248 SSRs, of which di-, triand hexa-nucleotide repeats were 352 (10.83%), 822 (25.30%), 17 (0.52%) respectively. A total of 527 primer pairs were designed for the annotated SSR-Contigs. About 77.07% SSR-ESTs could be assigned a significant match to the protein database. 210 unique SSRFDM (Functional Domain Markers) were assigned for significant functional domains by InterProScan. The gene ontology (GO) analysis provided 1213 number of unique GO terms which were subjected to enrichment analysis to obtain 95 statistically significant GO terms mapped to the SSR containing ESTs. Most frequent enriched GO terms were GO:0005886 for cellular component, GO:0003677 for molecular function and GO:0044255 in case of biological process, indicating the potential of the species as a fuel crop. Many SSR markers were functionally annotated to various biotic and abiotic stress responsive genes. Further studies may help us to understand these traits of extreme adaptive features. This will provide opportunity to genetically manage and modify crops for resistance to these stresses.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"53-63"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44602976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics differentiation of oil palm (Elaeis guineensis Jacq.) spear leaf with contrasting susceptibility to Ganoderma boninense","authors":"Nurul Liyana Rozali, M. Yarmo, A. S. Idris, A. Kushairi, U. S. Ramli","doi":"10.21475/POJ.10.02.17.PNE364","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE364","url":null,"abstract":"Basal stem rot (BSR) disease caused by Ganoderma boninense is the most serious and destructive disease in oil palm, especially in Southeast Asia and required urgent control measures to combat the disease outbreak. Information of understanding metabolite response of oil palm to BSR is limited. Therefore, parental palms with contrasting susceptibility to G. boninense based on previous oil palm progenies testing using root inoculation technique to identify oil palm progenies partially tolerant and susceptible to G. boninense were examined by metabolomics approach using gas chromatography x gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS). Analysis of metabolomics data from GC×GC-TOF-MS was conducted by supervised multivariate analysis of partial least squares-discriminant analysis (PLS) and orthogonal partial least squares-discriminant analysis (OPLS-DA) that allowed cross-validation and response permutation test functions. As a result, seven potential metabolites that contribute to the contrasting susceptibility of oil palms to G. boninense were identified as mannose, xylose, glucopyranose, myo-inositol and hexadecanoic acid which were found high in partially tolerant oil palm whereas cadaverine and turanose were found high in susceptible oil palm as observed in fold changes of detected GC×GC-TOF-MS peaks. The results suggest that the employed strategy is a potential approach to profile and characterize leaf metabolome with contrasting susceptibility to G. boninense. This result provide baseline in future studies utilizing metabolomics in identifying potential biomarkers by screening larger population of truly resistant palms to G. boninense.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44266734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary genome-wide association mapping of rice bacterial leaf blight resistance loci using major Korean races of Xoo ('Xanthomonas oryzae')","authors":"Asjad Ali, Do Yoon-Hyun, T. Noh, Yu-mi Choi, Sukyeung Lee, Sejong Oh, Myung-chul Lee","doi":"10.21475/POJ.10.02.17.PNE400","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE400","url":null,"abstract":"Bacterial leaf blight (BLB), caused by X. oryzae pv. oryzae (Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 96 accessions was used in the genomewide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. The results of the bioassay using a selected set of 96 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) and more than 4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice cultivars.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"97-106"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42749355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic sequence characterization of Begomovirus infecting soybean and molecular evolutionary genomics of Legume yellow mosaic viruses (LYMVs)","authors":"S. Ramesh, B. S. Chouhan, G. Gupta, S. M. Husain, S. Chand","doi":"10.21475/POJ.10.02.17.PNE391","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE391","url":null,"abstract":"Begomoviruses infecting legumes (family Geminiviridae) pose a serious threat to the cultivation of grain legumes. Eventhough legume yellow mosaic viruses (LYMVs) cause significant loss in yield of legumes, studies regarding evolutionary lineage analysis of LYMVs are very rare. Previously, we have shown that Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV) are major begomoviruses causing yellow mosaic disease (YMD) of soybean in India. In this study, complete genome sequence of begomovirus causing yellow mosaic disease of soybean in Central Indian region was characterized. Furthermore, whole genome sequences of legume begomoviruses [DNA A (108 isolates) and DNA B (89 isolates)] were analyzed to infer genetic diversity, gene flow and evolutionary lineage using nucleotide sequence-based computational approaches. Analysis of nucleotide diversity disclosed that LYMV population as a whole is diverse compared to MYMV and MYMIV. Test of neutral evolution also reiterates the operation of purifying selection and population expansion of MYMV and MYMIV. However, LYMVs as a whole, show decrease in population size and act of balancing or neutral selection. Genetic differentiation studies reveal greater diversity between MYMV and MYMIV. Frequent gene flow was detected between Dolichos yellow mosaic virus (DoYMV), Rhynchosia yellow mosaic India virus (RhYMIV) and other LYMVs. Recombinant events have been detected among LYMV species suggesting frequent genetic exchanges. Molecular phylogeny also revealed distinctness of Old World begomoviruses as New World begomoviruses formed a separate basal cluster. Hence, it is concluded that genetic exchanges are recorded among the LYMVs, and implications of breaching this seclusion is also discussed.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"88-96"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49500556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mineral deficiencies influence on tomato leaves: pigments, hydrogen peroxide and total phenolic compounds contents","authors":"K. Alsharafa","doi":"10.21475/POJ.10.02.17.PNE386","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE386","url":null,"abstract":"The detection of hydrogen peroxide, chlorophyll pigments, anthocynin, carotenoids, total phenolic compounds and lipid peroxidation levels as potential stress signaling molecules in tomato (Lycopersicon esculentum Mill.) leaves in response to specific mineral deficiency were studied. The stress signaling molecules were measured in the plant leaves at different growth time points cultured in specific mineral deficient nutrient solutions. The results showed that hydrogen peroxide was significantly increased after 48 and 72h of growth in NO3 ̄ and S deficient nutrient solutions. While the significant accumulation of H2O2 in the plant leaves was observed after 72h and 96h of growth in K deficient nutrient solution. In the case of Mg and Fe deficiency the significant accumulation was observed after 72h cultivation. The only significant reduction in chlorophyll a content was detected after 96h cultivation under Mg specific deficiency with no significant changes due to NO3 , SO4 , Fe, K, Ca and PO4 2 ̄ deficiencies. Meanwhile, chlorophyll b contents were significantly reduced in the plant leaves grown for 48h under Mg and Ca specific mineral deficiency. Longer cultivation in Mg dificient hydroponic culture for 96h resulted in 215% increment in chlorophyll b content. No significant changes in chlorophy b contents were detected in hydroponic cultures dificient in NO3 , SO4 , Fe, and PO4 . Impose of minerals deficiencies on anthocyanins and carotenoids contents were showed parallel changes. Both increased significantly in response to PO4 2 ̄ and NO3 ̄ specific deficiencies after 48h and in the case of Mg deficiency after 72h. Meanwhile, SO4 deficiency caused the significant increase of both after 72h and 96h. Regarding TPC the results clarified that Mg, Ca and K specific deficiencies caused significant reductions that appeared after 48h. In contrast, S deficiency caused significant increase in TPC values after 72h. On the other hand, the estimated levels of MDA showed significant increment under Ca and K and PO4 2 ̄ specific deficiencies at all time points while in the case of Mg and Fe deficiencies the increment was first reported after 48h and with the later one (Fe) the increment continues up to 96h. These results indicate that some of presented metabolites could be used as stress markers. These results support the possible role of anthocyanins, carotenoids, hydrogen peroxide, total phenolic compounds contents and MDA as early signaling metabolites in tomato plants under specific mineral deficiency.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"78-87"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45217091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Amplification of Start codon Polymorphic Loci (SASPL): a new PCR-based molecular marker in olive","authors":"A. Alsamman, S. Adawy, S. Ibrahim, B. Hussein, E. H. Hussein","doi":"10.21475/POJ.10.02.17.PNE385","DOIUrl":"https://doi.org/10.21475/POJ.10.02.17.PNE385","url":null,"abstract":"The Selective Amplification of Start Codon Polymorphic Loci (SASPL) has been developed as a new PCR-based molecular marker. SASPL was validated for the analysis of varietal diversity on ten olive varieties. Validation included in vitro comparison against RAPD, SCoT and SAMPL markers. Assessment of these techniques included primer selectivity, genome coverage and the ability to target genic regions through in silico PCR analysis. Candidate PCR fragments were further sequenced to annotate non-identified genes in olive. Eight SASPL primers were compared to 24 RAPD, 39 SCoT and 12 SAMPL primers. The TA produced by the RAPD, SCoT, SAMPL and the eight SASPL primers were 359, 642, 571 and 269 amplicons, respectively. The highest average number of TA was revealed by SAMPL (47.6), average of PA (18.1) and genetic similarity (GS) (96%) among the olive varieties. On the other hand, SASPL analysis provided higher average number of TA (33.6), average of PA (16.2) and GS (93%) than SCoT and RAPD. The highest average of (PIC) (0.2909) was exhibited by SASPL analysis and the lowest average (0.2038) was revealed by SCoT. The highest number of UB (111) was revealed by SCoT and the lowest UB (43) was obtained by SASPL. Across the four marker types, variety Maraki was characterized by the highest number of unique markers (74). Meanwhile, the variety Manzanillo was characterized by the lowest number of unique markers (8). In addition, in the in silico analysis SASPL exhibited the highest chromosomal coverage (0.59%) and targeted genes (1090) using the lowest number of primers. Additionally, the average area covered by the SASPL primers (354kb) was larger than SCoT and SAMPL. RAPD analysis provided the lowest potential, chromosomal coverage (0.04%) and number of targeted genes (17) compared to SASPL, SCoT and SAMPL analysis. The total coverage of the genome, revealed by combined data was higher (1.21%) than that of each technique separately. Meanwhile, the difference between the actual and the total genomic regions covered by the combined data was about 652kb. Our results suggested that the newly developed SASPL marker is the most adequately and each of the studied marker target different genomic areas, while some areas are shared. Two SCoT and one RAPD fragment were sequenced and showed a high similarity to genes of high physiological functions; such as cyclic plant-specific DNA-binding transcription factor, SANT domain and Copia-type retrotransposon.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"64-77"},"PeriodicalIF":0.0,"publicationDate":"2017-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47782394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing the role of sucrose in potato microtuber formation in vitro","authors":"M. S. Islam, M. Roni, A. Uddin, K. Shimasaki","doi":"10.21475/POJ.10.01.17.282","DOIUrl":"https://doi.org/10.21475/POJ.10.01.17.282","url":null,"abstract":"Sucrose is a necessary external carbon substrate for in vitro microtuber induction and development. In this study, we determined the correlation between sucrose and microtuber formation in potato by investigating the role and significance of sucrose in the medium and the origin of explants, as well as the potential function of sucrose in microtuber formation in vitro. Sucrose strongly influences microtuber induction, growth, and earliness without negative side effects. The results of this study show there was a significant correlation of R = 0.95 between tuberization and high sucrose content in the medium. High sucrose content in the medium is the carbon source that influences microtuber formation and development irrespective of the origin of explants. The present work could be considered efficient for large scale multiplication and propagation of this important vegetable crop in vitro. Hence, the results of this study should help rapid micropropagation of commercial potato cultivars by using high concentrations of sucrose in the microtuberization medium for a higher percentage of microtuber formation with earliness.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"15-19"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45538741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}