Plant Omics最新文献_第8页

Germination and seedling growth of barley as affected by Artemisia annua water extract 黄花蒿水提取物对大麦发芽和幼苗生长的影响

Plant Omics Pub Date : 2017-01-20 DOI: 10.21475/POJ.10.01.17.241

M. Salman, Naser M. Salameh, Saeid M Abu-Romman

{"title":"Germination and seedling growth of barley as affected by Artemisia annua water extract","authors":"M. Salman, Naser M. Salameh, Saeid M Abu-Romman","doi":"10.21475/POJ.10.01.17.241","DOIUrl":"https://doi.org/10.21475/POJ.10.01.17.241","url":null,"abstract":"Laboratory and greenhouse pot experiments were conducted to assess the allelopathic effects of Artemisia annua water extract on germination and growth of barley. Lower concentrations of A. annua water extract (0.5 and 1.0 %) did not affect the germination of barley seeds. However, higher concentrations (1.5-3.5 %) resulted in significant reductions in the germination percentage. Seedling growth of barley was also affected by A. annua water extract. Both shoot and root lengths were negatively affected by A. annua water extract and the degree of inhibition was concentration dependent. When barley seedlings were subjected to 0.5% extract concentration, shoot length was inhibited by 6% while root length showed 18.5% inhibition over control seedlings. The lowest shoot and root lengths were recorded at 3.0 and 3.5 % water extract. At 0.5 % extract concentration, shoot and root fresh and dry weights were significantly unaffected compared with the control. Shoot fresh weight was significantly unaltered when seedlings were treated with 1.0 % extract. However, at the same concentration root fresh weight, shoot dry weight, and root dry weight were inhibited by 44.7, 33.3, and 40 %, respectively. The present results confirmed that root growth (length and weight) was more sensitive to A. annua water extract than shoot growth. The present results also indicated the presence of water soluble allelochemicals in A. annua that are able to inhibit growth of barley.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47877396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Begomovirus infection on Cucumber in Saudi Arabia 沙特阿拉伯黄瓜的begomvirus感染

Plant Omics Pub Date : 2017-01-20 DOI: 10.21475/POJ.10.01.17.281

S. S. Sohrab, M. Yasir, S. El-Kafrawy

{"title":"Begomovirus infection on Cucumber in Saudi Arabia","authors":"S. S. Sohrab, M. Yasir, S. El-Kafrawy","doi":"10.21475/POJ.10.01.17.281","DOIUrl":"https://doi.org/10.21475/POJ.10.01.17.281","url":null,"abstract":"Cucurbits are an important vegetable crops and among them Cucumber (Cucumis sativus) used mainly as vegetables and salad. The cucumber crop was found to exhibit yellow mosaic symptoms grown in the natural field in Saudi Arabia. We collected naturally infected samples and detected the begomovirus infection by polymerase chain reaction and full length as well as betasatellites viral genome was cloned and sequenced. The sequences of the full length viral genome had 2784 and betasatellites had 1377 nucleotides respectively. In a multiple sequences analysis, highest homology was observed with Tomato yellow leaf curl virus previously reported from Jizan and Al-Qasim, Saudi Arabia. The betasatellites sequences were also analyzed but, interestingly the highest homology was observed with Tomato yellow leaf curl betasatellites reported from Jeddah and Oman. In a phylogenetic tree analysis, the closest cluster was formed with begomovirus isolates identified earlier from Jizan and Al-Qasim. Based on the results obtained in this study, it is concluded that a variant of Tomato yellow leaf curl virus associated with yellow mosaic disease of cucumber in Saudi Arabia. These findings provide valuable information about the natural infection and disease spread caused by begomovirus in new geographic regions on new host.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"7-14"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44464060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L. 番茄卷叶苏丹病毒（TLCSDV）在一个新的寄主紫外苋上引起卷叶病。

Plant Omics Pub Date : 2017-01-20 DOI: 10.21475/POJ.10.01.17.292

S. S. Sohrab

{"title":"Tomato Leaf Curl Sudan Virus (TLCSDV) causing leaf curl disease on a new host Amaranthus cruentus L.","authors":"S. S. Sohrab","doi":"10.21475/POJ.10.01.17.292","DOIUrl":"https://doi.org/10.21475/POJ.10.01.17.292","url":null,"abstract":"Amaranthus leaf curl disease symptom was observed in the farmer’s field based at Jeddah, Saudi Arabia. This crop used as leafy vegetables in many countries. In this study, a field survey was conducted in April 2014 and naturally infected Amaranthus leaf samples were collected to identify the associated virus with leaf curl disease. The causative agent was transmitted through whiteflies (Bemisia tabaci) from naturally infected leaves to healthy Amaranthus seedlings. The begomovirus infection was identified by PCR by using specific primers. The full viral genome was amplified by rolling circle amplification. The presence of betasatellites was also confirmed by using betasatellites specific primers. The full viral genomes as well as betasatellites were amplified, cloned and sequenced. The full-length viral genome sequence analysis showed the highest (99.9%) homology with Tomato leaf curl Sudan Virus infecting tomato reported from the Arabian Peninsula. The betasatellites sequence analysis showed the highest identity (99.3%) with Tomato leaf curl betasatellites-Yemen. The phylogenetic analysis was performed by using both full as well as betasatellites genome and full genome formed the closest cluster with Tomato leaf curl Sudan virus while betasatellites genome formed closed cluster with tomato yellow leaf curl Yemen betasatellites. The recombination analysis was performed and results showed that the associated virus could be a variant of Tomato leaf curl Sudan virus, a virus that occurs in Sudan, Yemen and Arabian Peninsula. This is the first report that about the Tomato leaf curl Sudan virus causing leaf curl disease on a new host Amaranthus in Jeddah, Saudi Arabia.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"20-27"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46373350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Quantitative analysis of antimicrobial activity of Foeniculum vulgare: A review 普通小茴香抗菌活性的定量分析综述

Plant Omics Pub Date : 2017-01-20 DOI: 10.21475/POJ.10.01.17.322

K. Al-Hadid

引用次数: 11

Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status 不同钾营养状况对番茄初级代谢产物组织特异性反应的影响

Plant Omics Pub Date : 2017-01-20 DOI: 10.21475/POJ.10.01.17.277

J. Sung, Hejin Yun, Min-Ji Cho, J. Lim, Seul-Bi Lee, Deog-Bae Lee, T. Oh

{"title":"Tissue-specific response of primary metabolites in tomato plants affected by different K nutrition status","authors":"J. Sung, Hejin Yun, Min-Ji Cho, J. Lim, Seul-Bi Lee, Deog-Bae Lee, T. Oh","doi":"10.21475/POJ.10.01.17.277","DOIUrl":"https://doi.org/10.21475/POJ.10.01.17.277","url":null,"abstract":"As one of the most important mineral nutrient elements, potassium (K) plays crucial roles in many fundamental processes, including enzyme activation, membrane transport, anion neutralization and osmo-regulation, and determines the yield and quality of crop production. In order to better understand and elucidate plant tissue-specific primary metabolic changes under different K nutrition status. Four-week-old tomato plants were subjected to different K nutrition situations: low (0.25 mM); normal (2.5 mM); and high (10.0 mM); and the emerging leaves, fully expanded leaves, petioles, stem and roots were harvested at 15 and 30 days, time points which the external symptoms are observed, after K treatments. Primary metabolites, amino acids, organic acids and sugars, extracted from each tomato tissue were measured with HPLC system. Several interesting findings from this study could be summarized as follows: (1) metabolites showed K-dependent responses, which indicated that the rates of an increase and decrease in low K-affected were 50 % : 50 % ;whereas, 80 % : 20 % in high K; (2) the petioles revealed the most sensitive plant tissue in response to K nutrition status; and (3) metabolites such as glucose and fructose (soluble sugars), malate and citrate (organic acids), and glutamine, asparagine, glutamate and aspartate (amino acids) strongly fluctuated (up or down) by the K nutrition ratio. These findings may contribute to a better understanding and elucidating the tissue-specific biosynthetic patterns and primary metabolite accumulation under different K nutrition ratios, and provide a new strategy for comprehensive information involved in the spatio-temporal metabolic networks","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47357556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular cloning, characterization and expression pattern analysis of a jasmonic acid responsive sesquiterpene synthase gene from Persicaria minor 小桃属茉莉酸响应倍半萜合成酶基因的克隆、表征及表达模式分析

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE271

Chew Jin Kiat, M. Ashraf, M. Naeem-ul-Hassan, C. Zain, Z. Zainal, I. Ismail

{"title":"Molecular cloning, characterization and expression pattern analysis of a jasmonic acid responsive sesquiterpene synthase gene from Persicaria minor","authors":"Chew Jin Kiat, M. Ashraf, M. Naeem-ul-Hassan, C. Zain, Z. Zainal, I. Ismail","doi":"10.21475/POJ.09.05.16.PNE271","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE271","url":null,"abstract":"Terpenoids represent an important class of plant secondary metabolites, serving as component of plant defense against various biotic and abiotic stresses. A large number of structurally diverse terpenoid compounds have been identified from Persicaria minor (P. minor), an aromatic plant native to Malaysia. However, number of studies focusing on the P. minor terpenoid metabolism, especially at the genetic level is still very scarce. In the present study, a cDNA sequence (KT192706) from the leaves of P. minor, encoding a sesquiterpene synthase (PmSS) gene was successfully cloned. The complete sequence of PmSS comprised of 1724 bp with a 1680 bp open reading frame, corresponding to a deduced protein of 559 amino acids. Under the normal conditions, PmSS gene was found to be differentially expressed in the organs of P. minor. Significantly higher expression level of PmSS was recorded in leaves and stems which are, respectively, about 98 folds and 49 folds higher than that in the roots. In addition, the present study has also shown that the expression of PmSS gene was responsive towards the exogenous application of jasmonic acid in all organs of the P. minor.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"360-368"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Transcriptome expression profiling in response to drought stress in Ilex paraguariensis leaves 干旱胁迫对巴拉圭冬青叶片转录组表达的影响

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE185

R. Acevedo, O. Ruiz, P. Sansberro

引用次数: 5

Salinity and drought-induced methylglyoxal detoxification in Brassica spp. and purification of a high active glyoxalase I from tolerant genotype 盐和干旱诱导的芸苔属植物甲基乙二醛解毒及抗性基因型高活性乙二醛酶I的纯化

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE232

M. Hossain, M. Hasanuzzaman, M. E. Hoque, H. Huq, M. Rohman

{"title":"Salinity and drought-induced methylglyoxal detoxification in Brassica spp. and purification of a high active glyoxalase I from tolerant genotype","authors":"M. Hossain, M. Hasanuzzaman, M. E. Hoque, H. Huq, M. Rohman","doi":"10.21475/POJ.09.05.16.PNE232","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE232","url":null,"abstract":"This experiment was conducted to study the role of glyoxalase system in conferring salinity and drought stress in Brassica spp. Two Brassica genotypes viz. BARI Sharisha16 (tolerant) and Tori7 (susceptible) were exposed to salt (16 dS m) and drought for 2, 4 and 6 days. The comparative study of two Brassica genotypes under salinity and drought stresses revealed that BARI Sharisha16 is more tolerant than Tori7 in both stresses. Under drought stress and salinity stress, Gly I activity increased significantly in both genotypes. Notably, concomitant increased activities of Gly I and Gly II with increased methylglyoxal (MG) suggested their role in MG detoxification in Brassica Spp. At six-day of salt stress, it was remarkable that Gly I and Gly II activities were 49 and 36 % higher in BARI Sharisha16 than Tori7. In addition, Gly I and Gly II activities were 24 and 21 % higher in BARI Sharisha16 than Tori7 after sixth day of drought, and hence, using different column chromatography Gly I was purified from BARI Sharisha16 seedlings. In purification, the fraction eluted from affinity chromatography showed specific activity of 173.51 μM minmg protein. In SDSPAGE, the purified Gly I protein migrated as a single band on with an apparent molecular mass of 27 kDa. In final purification, the recovery of Gly I activity was 0.38% along with purification fold 112.7. In this study, role of glyoxalase system in detoxification of MG was observed and subsequently, Gly I was purified from tolerant genotypes.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"352-359"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Expression analysis of five critical transcription factors (TFs) OsbHLH148, OsbZIP72, OsMYB2, OsNAC6 and TRAB1 in response to drought stress in contrasting Iranian rice genotypes 5个关键转录因子OsbHLH148、OsbZIP72、OsMYB2、OsNAC6和TRAB1在干旱胁迫下的表达分析

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE158

A. Taghipour, A. Tarang, N. Zare, Moslem Pourebrahim, R. Seighalani, Mahdi Ghasemi Selakjani

{"title":"Expression analysis of five critical transcription factors (TFs) OsbHLH148, OsbZIP72, OsMYB2, OsNAC6 and TRAB1 in response to drought stress in contrasting Iranian rice genotypes","authors":"A. Taghipour, A. Tarang, N. Zare, Moslem Pourebrahim, R. Seighalani, Mahdi Ghasemi Selakjani","doi":"10.21475/POJ.09.05.16.PNE158","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE158","url":null,"abstract":"Drought stress causes great damage to the rice cultivation all over the world. Specific transcription factors (TFs) can regulate the expression of stress-related genes. In this research, we investigated the effect of drought stress on the expression of five specific transcription factors OsbHLH148, OsbZIP72, OsMYB2, OsNAC6 and TRAB1 at vegetative and reproductive stages in two Iranian rice cultivars with drought-sensitive and tolerant backgrounds; Hashemi and Neda. Using a real-time quantitative PCR (qPCR) approach, this study revealed that the expression of OsbZIP72, OsMYB2 and OsNAC6 were increased significantly in the Hashemi cultivar under drought stress at the vegetative stage. It seems that these three genes paly their roles in the drought sensitive cultivar Hashemi at the vegetative stage and do not play any role at the reproductive stage as the most sensitive stage to drought stress. The expression of TRAB1 was increased in Hashemi cultivar at the reproductive stage, while the expression of TRAB1 was decreased in Neda cultivar at the vegetative stage. This indicates that the expression of TRAB1 could respond sensitively to drought stress at the vegetative stage. Furthermore, there were statistically significant increases in expression of OsbHLH148, OsbZIP72, OsMYB2, OsNAC6 and TRAB1 in Neda (tolerant) cultivar at the reproductive stage. Therefore, our study suggests that these five genes might be involved in drought tolerance of this cultivar to drought stress at the reproductive stage. Thus, they could be used as viable candidate TFs to develop additional varieties of drought-tolerant transgenic rice.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"10 1","pages":"327-333"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68657867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Proteomics analysis of the photo-oxidation mutant 812HS rice (Oryza sativa L.) exposed to high light intensity 高光强下水稻光氧化突变体812HS的蛋白质组学分析

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE80

C. Lv, Jing Ma, Z. Yuan, Yuwen Wang, Guoxiang Chen, Zhiping Gao, C. Lv

{"title":"Proteomics analysis of the photo-oxidation mutant 812HS rice (Oryza sativa L.) exposed to high light intensity","authors":"C. Lv, Jing Ma, Z. Yuan, Yuwen Wang, Guoxiang Chen, Zhiping Gao, C. Lv","doi":"10.21475/POJ.09.05.16.PNE80","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE80","url":null,"abstract":"The 812HS rice line, a mutant of two-line sterile rice, exhibits leaf photo-oxidation during the tillering-jointing stage since its chloroplasts are susceptible to damage induced by strong sunlight. To determine the proteomic response to light intensity, the 812HS rice line was exposed to natural light and shade (about one fourth natural light), and two-dimensional electrophoresis in combination with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry was used to compare the proteomic expressions in the rice leaves. Although over 1000 reproducible proteins were detected, only 9.61 % of them displayed differential expression with 1.5-fold abundance. A search of the National Center for Biotechnology Information database revealed 53 proteins, 34 down-regulated and 19 up-regulated under highlight. Among these identified proteins, six ones were related to disease and defense, implying the defense and protection mechanism was built under strong light stress. The increase in S-adenosylmethionine synthetase involved in the biosynthesis of the phytohormone ethylene might contribute to the phenotypic modulation from green to etiolation in photo-oxidation-sensitive leaves. These results suggest the existence of a complex regulatory mechanism of the proteomes in photo-oxidation-sensitive 812HS rice under light stress.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"304-310"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1