Plant Omics最新文献_第9页

In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis 桑树LRR受体样丝氨酸苏氨酸激酶亚家族的计算机分析

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE126

A. Zambounis, Fotis Psomopoulos, Ioannis Ganopoulos, E. Avramidou, F. Aravanopoulos, A. Tsaftaris, P. Madesis

{"title":"In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis","authors":"A. Zambounis, Fotis Psomopoulos, Ioannis Ganopoulos, E. Avramidou, F. Aravanopoulos, A. Tsaftaris, P. Madesis","doi":"10.21475/POJ.09.05.16.PNE126","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE126","url":null,"abstract":"Mulberries are important trees crops for orchards and agroforestry systems, which are plagued by many phytopathogenic fungal species. Leucine rich repeats (LRRs) receptor-like serine threonine kinases (LRR-RSTK) subfamily plays an important role in plant defense-related reactions against fungal attacks. In the present study, we mined this subfamily on Morus notabilis, a mulberry species whose relevant annotated genome assembly has recently become publicly available. Our aim was to decipher in silico the expansion and phylogeny of these genes, their homology relationships against their orthologous in woody angiosperm plant species, and the existence of positive selective pressures acting upon their LRRs. This subfamily was found to be quite abundant and diverged, comprising by 142 annotated gene members and containing a range of conserved functional domains in their C-termini, whilst their LRRs number ranged from one to 17 repeats. A phylogenetic investigation revealed 12 distinct clades based on their diverse structural profiles, mainly as a result of the fused functional domains at their C-termini. The interspecific expansion of these M. notabilis LRR-RSTKs has been investigated by a homology analysis across 12 other woody angiosperm species, showing that the highest proportion of homologous best BLAST hits observed primarily in Prunus persica, Malus domestica and Theobroma cacao. Using a series of maximum likelihood analyses, extensive episodes of positive selective pressures acting across the LRRs were observed. This overall evidence supports a potential crucial role of this diverged LRR-RSTK subfamily as a surveillance mechanism of M. notabilis against fungal attacks by providing rapidly evolving ligand-binding specificities.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"319-326"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68657778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers 部分埃及葡萄品种的遗传多样性、品种鉴定及SSR和SCoT标记的基因检测

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE125

S. Ibrahim, S. Adawy, M. Atia, A. Alsamman, Morad M. Mokhtar

{"title":"Genetic diversity, variety identification and gene detection in some Egyptian grape varieties by SSR and SCoT markers","authors":"S. Ibrahim, S. Adawy, M. Atia, A. Alsamman, Morad M. Mokhtar","doi":"10.21475/POJ.09.05.16.PNE125","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE125","url":null,"abstract":"Leaves of seven Egyptian grape varieties represent different range of agronomic and morphological traits were genotyped using Start-codon Targeted (SCoT) and Simple Sequence Repeats (SSR). The 24 SCoT primers generated 362 total fragments with 77.10% of polymorphism and 0.04 of average PIC. Dice coefficient that shows the genetic similarity and relationship was also used between the seven varieties. On one hand, SCoT analysis successfully characterized 73 unique positive and negative markers differentiating between the rootstock varieties especially those with green and red fruits. On the other hand, the seven SSR primers produced 73 fragments with 86.30% total polymorphism and 0.14 of average PIC. It also provided 19 unique positive and negative markers differentiating between the rootstock varieties. SO4 variety was identified by the highest number of positive unique markers (5). Both Scot and SSR analysis had covered 0.02% (0.10 Mbp) of V.vinifera genome. The SCoT covered 0.96 Mbp and SSR covered 846 bp. Together, the covered region encompassed about 22 genes of grape genome. Further, eight SCoT polymorphic bands were purified, cloned and sequenced. Of which, four SCoT sequences (SCoT3600, SCoT4450, SCoT6200 and SCoT12550) showed high similarity to some potential genes.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"311-318"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Comparative genomics and expression profile of lipid biosynthesis pathway genes in Camellia sinensis 油茶脂质生物合成途径基因的比较基因组学及表达谱分析

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE50

M. Gogoi, Afruza Zaman, S. Borchetia, T. Bandyopadhyay

{"title":"Comparative genomics and expression profile of lipid biosynthesis pathway genes in Camellia sinensis","authors":"M. Gogoi, Afruza Zaman, S. Borchetia, T. Bandyopadhyay","doi":"10.21475/POJ.09.05.16.PNE50","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE50","url":null,"abstract":"The lipid biosynthesis pathway is directly associated with lipid content in plants. The genes involved in lipid biosynthesis of tea plant (Camellia sinensis) were identified through in-silico mining of available transcriptomic data in public domain. Seventy five homologous genes in tea were identified through comparative genomics and 56 sequences of them showed more than 50% sequence similarity with the Arabidopsis reference sequences. The expression pattern of five key genes biotin carboxylase (BC), Acyl CoA: diacylglycerol acyltransferase (DGAT), phosphatidylinositol synthase (PIS), monogalactosyldiacylglycerol synthase (MGDGS) and glycerol 3 phosphate dehydrogenase (G3PDH) were analysed through qRT-PCR in roots, leaves, flowers, and seeds of three Tocklai released tea varieties (TV1, TV17 and TV20). Relative expression analysis showed that all the five genes were highly up-regulated in the seeds compared to the other parts of the studied tea varieties. Except biotin carboxylase, other four genes showed highest expression in TV1 seeds compared to TV17 and TV20. The overall increased expression of four key lipid biosynthesis genes give an indication that TV1 seeds may contain higher lipid content than the others in this study. DGAT, PIS and G3PDH also showed upregulation in the leaves and roots of some of the studied varieties which is an indication of possible involvement of lipid biosynthesis genes in various growth and developmental processes.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"295-303"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vascular expression of trehalose phosphate synthase1 (TPS1) induces flowering in Arabidopsis 海藻糖磷酸合酶1 (TPS1)的维管表达诱导拟南芥开花

Plant Omics Pub Date : 2016-09-20 DOI: 10.21475/POJ.09.05.16.PNE188

J. Ruiz-Salas, R. Ruiz-Medrano, M. Montes-Horcasitas, K. Agreda-Laguna, J. Hinojosa-Moya, B. Xoconostle‐Cazares

{"title":"Vascular expression of trehalose phosphate synthase1 (TPS1) induces flowering in Arabidopsis","authors":"J. Ruiz-Salas, R. Ruiz-Medrano, M. Montes-Horcasitas, K. Agreda-Laguna, J. Hinojosa-Moya, B. Xoconostle‐Cazares","doi":"10.21475/POJ.09.05.16.PNE188","DOIUrl":"https://doi.org/10.21475/POJ.09.05.16.PNE188","url":null,"abstract":"Trehalose and Trehalose-6-phosphate (T6P) have been described as signaling molecules involved in plant growth, development, carbon partitioning, flowering and response to biotic and abiotic stress. In order to investigate the role of T6P in vascular tissue signaling, Arabidopsis, plants expressing the TPS1 gene under the control of the actin depolymerizing factor 3 promoter (At5g59880) were generated by Agroinfiltration. Flower development and silique production occurred earlier in overexpressing lines compared to wild type (WT) control plants, while accumulation of TPS1 transcripts in overexpressing plants was considerably higher in silique, rosette and cauline leaves than in WT plants. Flowering Locus T (FT, AT1g65480) transcript was detected in rosette and cauline leaves in tps1 overexpressing lines, which could cause the observed early flowering. Trehalose levels were higher in rosette and cauline leaves in TPS1 lines, although no differences were observed in photosynthesis rate. These results suggest that the ectopic expression in the vasculature of the TPS1 gene induced trehalose accumulation in the vasculature, inducing flowering without compromising plant development.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"344-351"},"PeriodicalIF":0.0,"publicationDate":"2016-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Plant gene co-suppression; basis of the molecular machinery of interfering RNA 植物基因共抑制;干扰RNA分子机制的基础

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.P7802

Jorge Ricaño-Rodríguez, J. Adame-García, Carolina I. Patlas-Martínez, E. Hipólito-Romero, J. Ramos-Prado

{"title":"Plant gene co-suppression; basis of the molecular machinery of interfering RNA","authors":"Jorge Ricaño-Rodríguez, J. Adame-García, Carolina I. Patlas-Martínez, E. Hipólito-Romero, J. Ramos-Prado","doi":"10.21475/POJ.16.09.04.P7802","DOIUrl":"https://doi.org/10.21475/POJ.16.09.04.P7802","url":null,"abstract":"RNA interference (RNAi), also known as post-transcriptional gene silencing (PTGS) co-suppression, is considered one of the most significant discoveries in molecular biology during the last several years. First recognized in plants, the starting point for its historical overview begins in the late 1980s and early 1990s when researchers used genetic engineering to alter flower color. RNAi is considered a gene down-regulation mechanism demonstrated to exist in all eukaryotes, where small RNAs (of approximately 21-24 nucleotides in size) function to guide specific effector proteins (Argonaute protein family) to a target nucleotide sequence by complementary base pairing. Subsequently, the effector protein complex down-regulates the expression of a RNA or DNA target. Although the small RNAi-directed gene regulation system was independently discovered in plants, fungi, worms and mammalian cells, scientific attention has been focused mainly on the regulation of development, biotic and abiotic stress responses and genome stability through controlling plant gene expression. On the other hand, the small interfering (si) RNA-mediated RNA silencing also functions as a neutral antiviral defense mechanism. The purpose of this review is to provide an overview of the discovery and molecular characterization of RNAi in plants.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"261-269"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Gas exchange, photosystem II photochemistry, and the antioxidant system of longan plant (Dimocarpus longan Lour.) leaves in response to lead (Pb) stress 龙眼植物叶片在铅胁迫下的气体交换、光系统II光化学和抗氧化系统

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.PNE95

Yanna Wang, Yongyu Li, Cuilan Ma, D. Qiu

{"title":"Gas exchange, photosystem II photochemistry, and the antioxidant system of longan plant (Dimocarpus longan Lour.) leaves in response to lead (Pb) stress","authors":"Yanna Wang, Yongyu Li, Cuilan Ma, D. Qiu","doi":"10.21475/POJ.16.09.04.PNE95","DOIUrl":"https://doi.org/10.21475/POJ.16.09.04.PNE95","url":null,"abstract":"Longan is one of the most important subtropical fruit trees and a famous special product in south China. Increased fruit demand brings longan cultivation to Pb-affected regions. Seedlings of longan (cv. Wulongling) in pots with sands were irrigated daily for 30 d with a freshly prepared nutrient solution containing different concentrations of Pb(NO3)2 (0, 100 , 200 , 400 , 600 , 800 and 1000 mg L) to determine physiological and biochemical responses of longan seedlings to various levels of lead (Pb). The results indicated that Pb stress substantially inhibited the growth of longan plants and markedly declined in their dry biomass. However, when the plants were grown at 100 mg L Pb, the growth and dry biomass of the plants showed no significant difference from control. In addition, the chlorophyll a fluorescence and gas exchange parameters were correlated with the growth and yield response. Pb treatments increased the minimum fluorescence (Fo) and caused a decrease in maximum fluorescence (Fm), variable fluorescence (Fv), the maximum quantum efficiency of PSII photochemistry (Fv/Fm), trapped energy flux per cross section (CS) at t=0 (TRo/CSo), electron transport flux per CS at t=0 (ETo/CSo), dissipated energy flux per CS at t=0(DIo/CSo), and the amount of active PSII reaction centers (RCs) per CS at t = 0 (RC/CSo). Furthermore, Pb stress led to decreases in the protein contents, the activity of peroxidase (POD, EC 1.11.1.7) and the accumulation of proline and malondialdehyde (MDA), and enhanced superoxide dismutase activity (SOD, EC 1.15.1.1), whereas catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX,EC 1.11.1.11) were enhanced at low Pb levels and decreased under high Pb stress. Nonetheless, these changes were closely related to the severity of the Pb stress.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"240-247"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68658744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Establishing an association between molecular markers and sheath blight ('Rhizoctonia solani Kuhn') resistance in rice 建立分子标记与水稻纹枯病抗性之间的关系

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.P7887

Megha Joshi, P. Singh, Showkat A. Waza, Vineeta Singh, S. Goswami, Pallavi, Sujit Kumar, Anil Kumar Singh, N. Zaidi, U. Singh

{"title":"Establishing an association between molecular markers and sheath blight ('Rhizoctonia solani Kuhn') resistance in rice","authors":"Megha Joshi, P. Singh, Showkat A. Waza, Vineeta Singh, S. Goswami, Pallavi, Sujit Kumar, Anil Kumar Singh, N. Zaidi, U. Singh","doi":"10.21475/POJ.16.09.04.P7887","DOIUrl":"https://doi.org/10.21475/POJ.16.09.04.P7887","url":null,"abstract":"The use of multiple regression analysis to validate molecular markers associated with sheath blight resistance was carried out in 73 germplasm accessions of rice. Data on disease reaction was recorded as lesion size and used for calculation of AUDPC (Area Under Disease Progress Curve) and Percent Disease Index (PDI). A variation in AUDPC ranging from 217.78 to 793.33 with a mean value of 532.51 was recorded for the genotypes infected with sclerotia. PDI ranged from 28.89 to 84.44 with the mean values of 55.40 for infected lines. The AUDPC and PDI values for resistant check Tetep were found to be 432.70 and 45.22, respectively. Higher AUDPC and PDI values serve as an indication for susceptibility towards the disease reaction. The PIC value for the SSR loci varied from 0.225 to 0.743 with an average value of 0.47. Markers RM336, RM209, RM251 and RM224 were most informative on the basis of their high PIC values. UPGMA clustering based on molecular data and AUDPC were found to be in good agreement with each other. Twenty five germplasm accessions were grouped as common in cluster I of dendrogram generated by all alleles, cluster I of dendrogram constructed by Tetep specific alleles, and clusters I and II of the AUDPC dendrogram. Multiple regression analysis revealed that the two markers RM251175 and RM257150 may be considered as markers for association with low disease index (resistance to sheath blight) in rice. Amongst the germplasm accessions, IC383396 and IC426017 behaved as potentially resistant to sheath blight.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"281-288"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68659043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A computational study on genetic diversity of shatterproof1 (shp1) and shatterproof2 (shp2) genes in some members of Oleraceae and its molecular implications 部分甘蓝科植物shp1和shp2基因遗传多样性的计算研究及其分子意义

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.PNE103

Shachi Gahoi, Neetesh Pandey, B. Suresh, M. Grover, S. Marla, A. Rai

引用次数: 0

Drought sensitive maize inbred shows more oxidative damage and higher ROS scavenging enzymes, but not glyoxalases than a tolerant one at seedling stage 干旱敏感型玉米自交系在苗期表现出更大的氧化损伤和更高的活性氧清除酶，但在乙醛酶方面不高于耐旱型玉米自交系

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.PNE31

M. Rohman, S. Begum, M. Talukder, A. Akhi, M. Amiruzzaman, A. Ahsan, Z. Hossain

{"title":"Drought sensitive maize inbred shows more oxidative damage and higher ROS scavenging enzymes, but not glyoxalases than a tolerant one at seedling stage","authors":"M. Rohman, S. Begum, M. Talukder, A. Akhi, M. Amiruzzaman, A. Ahsan, Z. Hossain","doi":"10.21475/POJ.16.09.04.PNE31","DOIUrl":"https://doi.org/10.21475/POJ.16.09.04.PNE31","url":null,"abstract":"This study was undertaken to unveil the oxidative stress tolerance mechanism in maize seedlings under drought. The level of oxidative stress and involvement of antioxidant and glyoxalase systems were investigated in seedlings of two maize inbreds: P134, a relatively drought tolerant, and P142, a drought susceptible inbred subjected to water deficit for 7 days and then rewatered to reveal the mechanism of oxidative stress tolerance under drought. Water content, chlorophyll (Chl), reactive oxygen species (ROS), lipid peroxidation, methylglyoxal (MG), lipoxygenase (LOX) activity, enzymatic and non-enzymatic antioxidants and glyoxalases status were investigated in the uppermost fully expanded leaves. The superoxide (O2 ) generation rate, hydrogen peroxide (H2O2), lipid peroxidation and MG as well as LOX activity were higher in P142 throughout the drought period. Conversely, relative water content (RWC), Chl, carotenoid (Car) and proline contents were remarkably higher in P134. However, in rewatering, recovery of Chl and Car were higher in P142. The reduced glutathione (GSH), ascorbic acid (ASA) and their redox homeostasis indicated more oxidative damage in P142. The ROS scavenging enzymes like superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) activities were comparatively higher in P142 under drought, while catalase (CAT), monodehydroasacorbate reductase (MDHAR) and glutathione reductase (GR) activities were higher in P134. Though the activity of GST increased in both inbreds, activities of glyoxalase-I (Gly-I) and glyoxalse-II (Gly-II) increased only in P134. In rewatering, activities of most of the enzymes decreased in both inbreds. Taken together, the non-enzymatic antioxidant system was stronger in P134, but the higher SOD, POD, APX, GPX and DHAR activities in P142 suggesting that these enzymes might involve in cellular protection through reducing oxidative damage.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"220-232"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68659159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

GC-MS/Olfactometric characterisation and aroma extraction dilution analysis of aroma active compounds in Polygonum minus essential oil 气相色谱-质谱联用/嗅觉表征及蓼减精油香气活性物质提取稀释分析

Plant Omics Pub Date : 2016-08-20 DOI: 10.21475/POJ.16.09.04.P7901

N. A. Rusdi, H. Goh, S. Baharum

{"title":"GC-MS/Olfactometric characterisation and aroma extraction dilution analysis of aroma active compounds in Polygonum minus essential oil","authors":"N. A. Rusdi, H. Goh, S. Baharum","doi":"10.21475/POJ.16.09.04.P7901","DOIUrl":"https://doi.org/10.21475/POJ.16.09.04.P7901","url":null,"abstract":"Polygonum minus Huds. is an aromatic plant with potential anti-inflammatory, antiulcer and diuretic properties. Although the volatile compounds from the essential oil of P. minus have been extensively studied, knowledge of the aroma-active compounds is still incomplete. Hence, the aim of this study is to investigate and to characterise the aroma-active compounds that contribute to the potent odour of P. minus. Essential oil was extracted from the leaves of kesum (P. minus) using hydrodistillation and was analysed by gas chromatography-mass spectrometry (GC-MS) to identify the volatile constituents. The important aroma-active compounds were further analysed by GC-MS/Olfactometry (GC-MS/O) and aroma extraction dilution analysis (AEDA). The GC-MS analysis using Kovats retention indices detected 39 volatile compounds in the essential oil. Aldehydes represented the major class of compounds, encompassing 75.43% of the total peak area, followed by sesquiterpenes (13.19%), alcohols (8.07%) and organic acids (0.83%). The dominant components of the kesum essential oil were dodecanal (54.25%) and decanal (18.27%) in the aldehyde group. The sesquiterpenes that were detected include β-farnesene (1.74%), α-caryophyllene (1.72%), β-caryophyllene oxide (1.51%) and βselinene (1.36%). In this study, GC-MS/O and AEDA analysis identified dodecanal, decanal, farnesol, 1-nonanal and α-bergamotene as the main contributors to the characteristic fragrance of this plant. This is the first olfactometric study on the essential oil of P. minus, and it is important for our understanding of the biosynthesis of volatile compounds in this medicinal herb. Moreover, the characterisation of aromatic compounds that contributed to the potent odour of P. minus can be further applied in the flavour and fragrance industries.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"9 1","pages":"289-291"},"PeriodicalIF":0.0,"publicationDate":"2016-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68659102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14