蔗糖在马铃薯试管苗形成中的作用

Q3 Agricultural and Biological Sciences
M. S. Islam, M. Roni, A. Uddin, K. Shimasaki
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引用次数: 7

摘要

蔗糖是体外微块茎诱导和发育所必需的外部碳底物。本研究通过研究蔗糖在培养基中的作用和意义以及外植体的来源,以及蔗糖在离体微块茎形成中的潜在作用,确定了蔗糖与马铃薯微块茎形成的相关性。蔗糖对微块茎的诱导、生长和早期发育有强烈的影响,而且没有负面的副作用。本研究结果表明,培养基中高蔗糖含量与结核形成呈显著相关,R = 0.95。培养基中的高蔗糖含量是影响微块茎形成和发育的碳源,无论外植体的来源如何。本研究为这一重要蔬菜作物的大规模离体繁殖提供了有效的方法。因此,本研究的结果应该有助于商业马铃薯品种的快速微繁,在微块茎培养基中使用高浓度蔗糖,以提高微块茎形成的百分比,并提前形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tracing the role of sucrose in potato microtuber formation in vitro
Sucrose is a necessary external carbon substrate for in vitro microtuber induction and development. In this study, we determined the correlation between sucrose and microtuber formation in potato by investigating the role and significance of sucrose in the medium and the origin of explants, as well as the potential function of sucrose in microtuber formation in vitro. Sucrose strongly influences microtuber induction, growth, and earliness without negative side effects. The results of this study show there was a significant correlation of R = 0.95 between tuberization and high sucrose content in the medium. High sucrose content in the medium is the carbon source that influences microtuber formation and development irrespective of the origin of explants. The present work could be considered efficient for large scale multiplication and propagation of this important vegetable crop in vitro. Hence, the results of this study should help rapid micropropagation of commercial potato cultivars by using high concentrations of sucrose in the microtuberization medium for a higher percentage of microtuber formation with earliness.
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来源期刊
Plant Omics
Plant Omics 生物-植物科学
CiteScore
1.30
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.
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